FastQCFastQC Report
Sat 18 Jun 2016
SRR3548776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645989
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC123511.9119520611032077No Hit
AATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT118551.835170567919887No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGT115181.7830024969465423No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT58880.9114706287568364No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27140.42013099294260425No Hit
AATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTT26090.40387684620016745No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT22230.3441235067470189No Hit
AACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTG18720.2897882162080159No Hit
AAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT13610.2106847020614902No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTAT12860.19907459724546392No Hit
AATGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC9780.15139576680098268No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTT9070.14040486757514448No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG253.888901E-545.0000041
CTATACG253.888901E-545.0000041
TAGCGGA253.888901E-545.0000043
ATATCGC253.888901E-545.00000414
ACGTGAC253.888901E-545.00000435
TCGTCAG253.888901E-545.00000429
AGTCTCG253.888901E-545.00000430
CATACCG253.888901E-545.0000041
GGCGATA253.888901E-545.00000445
CGATCCG253.888901E-545.0000041
CGTCAGT253.888901E-545.00000430
TATCGCC253.888901E-545.00000415
TGAGACG253.888901E-545.0000041
TCGCGCG253.888901E-545.0000041
CGGGTAT406.8066583E-945.06
CCGGGTA207.0309895E-445.05
TCCGCAG207.0309895E-445.01
CGCGGTA207.0309895E-445.04
ACGCATT207.0309895E-445.023
ACGTTGC207.0309895E-445.019