##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645989
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.416883259622068	31.0	31.0	34.0	30.0	34.0
2	31.593511654223214	31.0	31.0	34.0	30.0	34.0
3	31.7344691627876	31.0	31.0	34.0	30.0	34.0
4	35.52282159603337	37.0	35.0	37.0	33.0	37.0
5	35.13569426104779	37.0	35.0	37.0	32.0	37.0
6	35.13042482147529	37.0	35.0	37.0	32.0	37.0
7	35.13165704059976	37.0	35.0	37.0	32.0	37.0
8	35.45818891652954	37.0	35.0	37.0	33.0	37.0
9	36.3593652523495	39.0	37.0	39.0	32.0	39.0
10	35.94046493051739	38.0	35.0	39.0	30.0	39.0
11	36.26593951290192	38.0	35.0	39.0	32.0	39.0
12	36.08125370555845	37.0	35.0	39.0	32.0	39.0
13	36.06258775304224	37.0	35.0	39.0	32.0	39.0
14	37.0620846485002	39.0	36.0	40.0	32.0	41.0
15	37.08932969446848	39.0	35.0	40.0	32.0	41.0
16	37.008802007464524	38.0	35.0	40.0	32.0	41.0
17	36.96294673748314	38.0	35.0	40.0	32.0	41.0
18	36.72347671554779	38.0	35.0	40.0	31.0	41.0
19	36.780666543857556	39.0	35.0	40.0	31.0	41.0
20	36.854523838641214	39.0	35.0	40.0	31.0	41.0
21	37.00030031471124	39.0	35.0	40.0	32.0	41.0
22	36.968711541527796	39.0	35.0	40.0	32.0	41.0
23	36.95770980620413	39.0	35.0	40.0	32.0	41.0
24	36.58301611946953	38.0	35.0	40.0	31.0	41.0
25	36.62625679384634	38.0	35.0	40.0	31.0	41.0
26	36.570212495878415	38.0	35.0	40.0	31.0	41.0
27	36.48737672003703	38.0	35.0	40.0	31.0	41.0
28	36.395412305782294	38.0	35.0	40.0	30.0	41.0
29	36.199001840588615	38.0	35.0	40.0	30.0	41.0
30	36.11483322471435	38.0	35.0	40.0	30.0	41.0
31	36.03305938646014	38.0	35.0	40.0	30.0	41.0
32	35.76823908766249	38.0	34.0	40.0	29.0	41.0
33	35.89963760992834	38.0	35.0	40.0	30.0	41.0
34	35.89821343707091	38.0	35.0	40.0	30.0	41.0
35	35.73507134022406	38.0	34.0	40.0	29.0	41.0
36	35.80255700948468	38.0	35.0	40.0	30.0	41.0
37	35.49452544857575	38.0	34.0	40.0	27.0	41.0
38	35.612727151700724	38.0	34.0	40.0	29.0	41.0
39	35.41546682683451	38.0	34.0	40.0	27.0	41.0
40	35.41030110419837	38.0	34.0	40.0	28.0	41.0
41	35.56663193955315	38.0	34.0	40.0	29.0	41.0
42	35.556846943214204	38.0	34.0	40.0	29.0	41.0
43	35.54021662907573	38.0	34.0	40.0	29.0	41.0
44	35.3812758421583	38.0	34.0	40.0	28.0	41.0
45	35.06731848375127	38.0	34.0	40.0	27.0	41.0
46	34.99614854122903	37.0	34.0	40.0	26.0	41.0
47	34.90226149361676	37.0	34.0	40.0	26.0	41.0
48	34.90565319223702	37.0	34.0	40.0	26.0	41.0
49	34.778575176976695	37.0	34.0	40.0	26.0	41.0
50	34.49646975412894	37.0	33.0	40.0	24.0	41.0
51	32.478413719119054	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	9.0
11	12.0
12	11.0
13	11.0
14	23.0
15	40.0
16	87.0
17	181.0
18	383.0
19	659.0
20	1109.0
21	1765.0
22	2436.0
23	3248.0
24	3934.0
25	4692.0
26	5667.0
27	7379.0
28	9501.0
29	12317.0
30	16438.0
31	21550.0
32	28475.0
33	38229.0
34	58169.0
35	66262.0
36	67112.0
37	89393.0
38	116767.0
39	90122.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.81425225506936	38.585486749774375	18.83700806050877	23.763252934647493
2	20.91583602816766	37.12354235134035	20.42449639235343	21.53612522813856
3	19.555131743729383	30.17094718331117	26.96949948064131	23.304421592318135
4	24.180287899639158	30.406864513172827	18.263778485392166	27.149069101795853
5	16.351981225686504	38.3478666045397	18.869516353993642	26.430635815780146
6	20.720476664463327	34.143151044367634	24.64097685873908	20.495395432429962
7	76.98877225463592	10.3557490917028	7.572729566602527	5.082749087058758
8	79.12270951982154	4.636766260725802	4.98924904294036	11.251275176512292
9	74.11813513852404	10.276026371966086	9.901406989902304	5.704431499607578
10	34.351823328261005	37.94197734017143	13.529022940019104	14.177176391548462
11	25.641303489687907	23.481514391111922	32.63956506999345	18.23761704920672
12	26.26515312180238	22.705030581016086	35.11143378602422	15.918382511157311
13	23.10070295314626	30.94216774589041	26.605096990815635	19.352032310147695
14	15.921014134915609	31.939243547490747	34.40863544116076	17.731106876432882
15	14.570371941317886	30.906099020261955	31.999461291136537	22.52406774728362
16	17.23744521965544	26.929870322869277	34.993010716900756	20.83967374057453
17	16.04562925994096	28.07555546611475	29.911190438227276	25.967624835717018
18	18.987320217526925	27.789946887640504	28.99554017173667	24.227192723095904
19	19.349400686389398	31.221274665667682	31.396045443498267	18.033279204444657
20	22.838933789894256	31.822678095137842	26.53621036890721	18.802177746060693
21	19.236705268975168	28.65358388455531	28.692593836737156	23.41711700973236
22	18.400777722221275	30.549746203108725	28.34676751461712	22.70270856005288
23	20.87760008297355	27.89691465334549	24.33787572234202	26.887609541338943
24	16.445016865612264	34.223957373887174	28.094750839410576	21.236274921089986
25	17.66980552300426	28.752347176190305	27.847533007527993	25.73031429327744
26	22.101924336172907	32.42501033299329	23.93523728732223	21.537828043511578
27	16.615762807106623	30.725445789324585	29.2689194398047	23.38987196376409
28	21.31088919470765	30.94866940458739	27.173992126800922	20.566449273904045
29	21.341539871421958	30.174817218249846	28.8721634578917	19.611479452436498
30	22.736764867513223	31.477625780005543	24.54190396430899	21.243705388172245
31	28.194288138033308	29.56582852029988	21.6999051067433	20.539978234923506
32	22.829955308836528	27.689016376439845	26.97476272815791	22.506265586565714
33	28.03995114467893	28.991979739593088	23.77006419613956	19.198004919588413
34	21.554546594446656	26.194099280328302	25.894403774677276	26.356950350547763
35	21.58287525019776	29.783788888046082	29.21102371712212	19.42231214463404
36	28.54243648111655	24.677355187162632	27.896605050550395	18.883603281170423
37	21.08766557944485	30.144785747125724	26.83327425079994	21.93427442262949
38	27.939330236273374	26.419954519349403	27.15990519962414	18.480810044753085
39	21.00097679681852	24.76094794183802	31.296353343477985	22.941721917865472
40	25.451516976295263	26.222892340272047	25.95462151832307	22.37096916510962
41	18.984533792371078	23.377023447767687	32.522999617640544	25.115443142220688
42	24.38880538213499	25.09005571302298	27.69768525470248	22.82345365013955
43	22.87980135884667	24.82271369945928	29.149257959500858	23.148226982193197
44	21.506093757014437	24.890516711584873	27.312848980400595	26.290540551000095
45	26.1891456356068	28.017659743432166	25.324270227511615	20.468924393449424
46	19.933466359334293	24.550727643969168	34.37721075745872	21.13859523923782
47	22.697290511138736	27.161917617792252	25.45275538747564	24.688036483593372
48	21.13085516936047	21.915543453526297	34.42117435436207	22.53242702275116
49	22.58598830630243	21.946813335830797	31.21043856783939	24.256759790027385
50	20.52620091054182	23.97471164369672	26.789620256691677	28.70946718906978
51	19.010076022966334	23.114944681720587	32.78213715713426	25.092842138178824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3226.0
1	2598.5
2	1971.0
3	1397.0
4	823.0
5	783.0
6	743.0
7	782.5
8	822.0
9	923.0
10	1024.0
11	1121.5
12	1219.0
13	1266.5
14	1314.0
15	1510.0
16	1706.0
17	1636.5
18	1567.0
19	1531.5
20	1496.0
21	1783.5
22	2071.0
23	2432.5
24	2794.0
25	3731.0
26	5397.0
27	6126.0
28	7431.0
29	8736.0
30	9800.5
31	10865.0
32	11716.0
33	12567.0
34	14802.0
35	17037.0
36	19839.5
37	22642.0
38	23881.0
39	25120.0
40	28971.5
41	32823.0
42	37212.5
43	41602.0
44	63063.0
45	84524.0
46	83624.5
47	82725.0
48	76158.0
49	69591.0
50	61856.5
51	54122.0
52	47529.0
53	40936.0
54	35215.0
55	29494.0
56	25129.0
57	20764.0
58	19231.0
59	17698.0
60	16179.0
61	14660.0
62	13066.0
63	11472.0
64	9010.0
65	6548.0
66	5275.5
67	4003.0
68	3055.5
69	2108.0
70	1825.0
71	1542.0
72	1323.5
73	1105.0
74	947.0
75	581.0
76	373.0
77	349.0
78	325.0
79	201.0
80	77.0
81	71.0
82	65.0
83	52.0
84	39.0
85	34.5
86	30.0
87	22.5
88	15.0
89	10.5
90	6.0
91	7.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	645989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.188799143079763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6175895378883	21.87946641453543
2	9.778124123395726	5.5126715382101
3	3.374446117500576	2.8536475147610725
4	1.6329405159863406	1.8412252887094391
5	0.9385698192709254	1.3228578058592395
6	0.6091869613638623	1.0303349336681402
7	0.4465802467350139	0.8811992613536216
8	0.3463951825060873	0.7811571379035641
9	0.2876319452727924	0.7297199219185251
>10	3.683451566015176	24.75090370535496
>50	1.0597841536674	20.975185017758065
>100	0.21709716666974055	8.591330715433317
>500	0.002734221242691946	0.5760541659233915
>1k	0.0032810654912303356	1.8598055958966333
>5k	5.468442485383893E-4	0.9076282924690741
>10k+	0.0016405327456151678	5.506812690245448
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	12351	1.9119520611032077	No Hit
AATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	11855	1.835170567919887	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGT	11518	1.7830024969465423	No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT	5888	0.9114706287568364	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2714	0.42013099294260425	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTT	2609	0.40387684620016745	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	2223	0.3441235067470189	No Hit
AACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTG	1872	0.2897882162080159	No Hit
AAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	1361	0.2106847020614902	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTAT	1286	0.19907459724546392	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	978	0.15139576680098268	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTT	907	0.14040486757514448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5480139754701705E-4	0.0	0.0	1.2060576882888099	0.0
2	1.5480139754701705E-4	0.0	0.0	1.507301207915305	0.0
3	1.5480139754701705E-4	0.0	0.0	2.205455510852352	0.0
4	1.5480139754701705E-4	0.0	0.0	5.950565721707336	0.0
5	1.5480139754701705E-4	0.0	0.0	6.388034471175206	0.0
6	1.5480139754701705E-4	0.0	0.0	8.136361455071217	0.0
7	1.5480139754701705E-4	0.0	0.0	9.521988764514566	0.0
8	1.5480139754701705E-4	0.0	0.0	10.26859590488383	0.0
9	1.5480139754701705E-4	0.0	0.0	13.270814208910679	0.0
10	1.5480139754701705E-4	0.0	0.0	14.806908476769728	0.0
11	1.5480139754701705E-4	0.0	0.0	18.482822462921195	0.0
12	1.5480139754701705E-4	0.0	0.0	19.3675124499024	0.0
13	1.5480139754701705E-4	0.0	0.0	19.82402177126855	0.0
14	1.5480139754701705E-4	0.0	0.0	20.392142900266105	0.0
15	1.5480139754701705E-4	0.0	0.0	20.733015577664634	0.0
16	1.5480139754701705E-4	0.0	0.0	21.262281555877887	0.0
17	1.5480139754701705E-4	0.0	0.0	22.01987959547299	0.0
18	1.5480139754701705E-4	0.0	0.0	23.052714519906687	0.0
19	1.5480139754701705E-4	0.0	0.0	23.476870349185514	0.0
20	1.5480139754701705E-4	0.0	0.0	23.876722359049456	0.0
21	1.5480139754701705E-4	0.0	0.0	24.305057826062054	0.0
22	1.5480139754701705E-4	0.0	0.0	24.8059951485242	0.0
23	1.5480139754701705E-4	0.0	0.0	25.27210215653827	0.0
24	1.5480139754701705E-4	0.0	0.0	25.5948630704238	0.0
25	1.5480139754701705E-4	0.0	0.0	25.87876883352503	0.0
26	1.5480139754701705E-4	0.0	0.0	26.156637342121925	0.0
27	1.5480139754701705E-4	0.0	0.0	26.47041977494973	0.0
28	1.5480139754701705E-4	0.0	0.0	26.790703866474505	0.0
29	1.5480139754701705E-4	0.0	0.0	27.090399372125532	0.0
30	1.5480139754701705E-4	0.0	0.0	27.56223403184884	0.0
31	1.5480139754701705E-4	0.0	0.0	27.86223914029496	0.0
32	1.5480139754701705E-4	0.0	0.0	28.19320452825048	0.0
33	1.5480139754701705E-4	0.0	0.0	28.472001845232658	0.0
34	1.5480139754701705E-4	0.0	0.0	28.757610423706904	0.0
35	1.5480139754701705E-4	0.0	0.0	29.08594418790413	0.0
36	1.5480139754701705E-4	0.0	0.0	29.383782076784588	0.0
37	1.5480139754701705E-4	0.0	0.0	29.694158878866357	0.0
38	1.5480139754701705E-4	0.0	0.0	29.973575401438723	0.0
39	1.5480139754701705E-4	0.0	0.0	30.26135119947863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	25	3.888901E-5	45.000004	1
CTATACG	25	3.888901E-5	45.000004	1
TAGCGGA	25	3.888901E-5	45.000004	3
ATATCGC	25	3.888901E-5	45.000004	14
ACGTGAC	25	3.888901E-5	45.000004	35
TCGTCAG	25	3.888901E-5	45.000004	29
AGTCTCG	25	3.888901E-5	45.000004	30
CATACCG	25	3.888901E-5	45.000004	1
GGCGATA	25	3.888901E-5	45.000004	45
CGATCCG	25	3.888901E-5	45.000004	1
CGTCAGT	25	3.888901E-5	45.000004	30
TATCGCC	25	3.888901E-5	45.000004	15
TGAGACG	25	3.888901E-5	45.000004	1
TCGCGCG	25	3.888901E-5	45.000004	1
CGGGTAT	40	6.8066583E-9	45.0	6
CCGGGTA	20	7.0309895E-4	45.0	5
TCCGCAG	20	7.0309895E-4	45.0	1
CGCGGTA	20	7.0309895E-4	45.0	4
ACGCATT	20	7.0309895E-4	45.0	23
ACGTTGC	20	7.0309895E-4	45.0	19
>>END_MODULE