SRR3548774_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453418
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	4042	0.8914511554459681	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	3958	0.8729252036751959	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGT	3429	0.756255816928309	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3413	0.7527270642100667	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	3277	0.7227326661050069	RNA PCR Primer, Index 13 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1540	0.3396424491308241	Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	1059	0.23355932053866407	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	854	0.18834717633618428	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	853	0.18812662929129412	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	695	0.15328019619865113	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	683	0.15063363165996937	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	657	0.1448994084928256	No Hit
AACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	605	0.13343096215853803	RNA PCR Primer, Index 13 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	558	0.12306525104870121	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	489	0.10784750495128116	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTC	30	2.1625838E-6	45.000004	34
TGAACCG	30	2.1625838E-6	45.000004	14
TTCGTAG	35	1.2097553E-7	45.000004	32
ATCAAAT	30	2.1625838E-6	45.000004	42
TGACCGG	30	2.1625838E-6	45.000004	2
TCCAGCG	35	1.2097553E-7	45.000004	1
GTTCGTA	35	1.2097553E-7	45.000004	31
CGTAGGC	35	1.2097553E-7	45.000004	34
CCTTTCG	30	2.1625838E-6	45.000004	16
TCTAAGC	30	2.1625838E-6	45.000004	15
AGTTCGT	35	1.2097553E-7	45.000004	30
GGTTGCG	35	1.2097553E-7	45.000004	17
AATAGCG	30	2.1625838E-6	45.000004	1
CAATCGC	30	2.1625838E-6	45.000004	15
ATGCACG	35	1.2097553E-7	45.000004	1
AGTACGG	30	2.1625838E-6	45.000004	2
ATCGAGT	30	2.1625838E-6	45.000004	32
AATCGCT	30	2.1625838E-6	45.000004	16
TGCAACG	75	0.0	45.000004	1
TGATCGC	20	7.0287636E-4	45.0	18