SRR3548773_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461288
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	3774	0.8181439794661902	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	3687	0.7992837446454275	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3567	0.773269627651272	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGT	3353	0.7268777856783615	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	3156	0.6841712769462895	RNA PCR Primer, Index 13 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1340	0.29049097310140304	Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	1034	0.22415497476630653	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.19315481868160456	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	780	0.16909176046201072	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	704	0.1526161530323789	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.15239936872409426	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	658	0.14264407485128597	No Hit
AACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	605	0.13115450651220062	RNA PCR Primer, Index 13 (95% over 23bp)
AATGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	569	0.12335027141395397	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	535	0.11597960493227658	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGC	20	7.028889E-4	45.000004	21
TAATACG	20	7.028889E-4	45.000004	1
GTCGAGT	20	7.028889E-4	45.000004	19
GGGTCTA	20	7.028889E-4	45.000004	7
TGGACGT	35	1.2098099E-7	45.000004	31
GGCGTAC	20	7.028889E-4	45.000004	43
GACCGAC	40	6.7993824E-9	45.000004	43
GCACGAC	20	7.028889E-4	45.000004	40
CGTCTCA	20	7.028889E-4	45.000004	28
GTCTAGC	20	7.028889E-4	45.000004	9
AGGCGTA	20	7.028889E-4	45.000004	23
CATTCTC	20	7.028889E-4	45.000004	28
GAATAAG	20	7.028889E-4	45.000004	17
CGACAGA	20	7.028889E-4	45.000004	37
TAACGAA	20	7.028889E-4	45.000004	18
TCGATGG	40	6.7993824E-9	45.000004	2
GCGTTCC	20	7.028889E-4	45.000004	37
TAGCCGT	35	1.2098099E-7	45.000004	44
AGAACCG	75	0.0	45.000004	1
CCCACTA	20	7.028889E-4	45.000004	26