##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461288
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23180529300567	31.0	31.0	34.0	28.0	34.0
2	31.429406357850194	31.0	31.0	34.0	30.0	34.0
3	31.4040664400548	31.0	31.0	34.0	28.0	34.0
4	35.41269445552453	37.0	35.0	37.0	33.0	37.0
5	35.058681344409564	37.0	35.0	37.0	32.0	37.0
6	35.11756213038275	37.0	35.0	37.0	32.0	37.0
7	35.020581502228545	37.0	35.0	37.0	32.0	37.0
8	35.377096304261116	37.0	35.0	37.0	33.0	37.0
9	35.86496288652642	39.0	37.0	39.0	32.0	39.0
10	35.75505974575536	38.0	35.0	39.0	30.0	39.0
11	36.15806394269957	38.0	35.0	39.0	32.0	39.0
12	35.74741159535908	37.0	35.0	39.0	31.0	39.0
13	35.4258662700959	37.0	35.0	39.0	30.0	39.0
14	36.402767902048176	38.0	35.0	40.0	31.0	41.0
15	36.55098550146546	38.0	35.0	40.0	31.0	41.0
16	36.60144421706179	38.0	35.0	40.0	31.0	41.0
17	36.557764346785525	38.0	35.0	40.0	31.0	41.0
18	36.40708624546921	38.0	35.0	40.0	31.0	41.0
19	36.40242755068417	38.0	35.0	40.0	31.0	41.0
20	36.33987227068556	38.0	35.0	40.0	31.0	41.0
21	36.36192140268119	38.0	35.0	40.0	31.0	41.0
22	36.31754782261841	38.0	35.0	40.0	31.0	41.0
23	36.28098281334004	38.0	35.0	40.0	31.0	41.0
24	35.91878826243041	38.0	34.0	40.0	30.0	41.0
25	36.04319210558263	38.0	34.0	40.0	30.0	41.0
26	35.93988571131267	38.0	34.0	40.0	30.0	41.0
27	35.83026655798547	38.0	34.0	40.0	30.0	41.0
28	35.67201618078077	38.0	34.0	40.0	29.0	41.0
29	35.386649988727214	37.0	34.0	40.0	28.0	41.0
30	35.42746180260488	37.0	34.0	40.0	29.0	41.0
31	35.19352551984877	37.0	34.0	40.0	27.0	41.0
32	35.14773633825289	37.0	34.0	40.0	27.0	41.0
33	35.007112693154816	37.0	34.0	40.0	27.0	41.0
34	34.98468418861969	37.0	34.0	40.0	27.0	41.0
35	34.912330257886616	37.0	34.0	40.0	27.0	41.0
36	34.666798182480356	37.0	33.0	40.0	25.0	41.0
37	34.51872799639271	36.0	33.0	40.0	25.0	41.0
38	34.34253654983438	36.0	33.0	40.0	25.0	41.0
39	34.172558141551484	36.0	33.0	40.0	24.0	41.0
40	34.052945231612355	36.0	33.0	40.0	24.0	41.0
41	34.15823303446003	36.0	33.0	40.0	24.0	41.0
42	34.176128579108926	36.0	33.0	40.0	24.0	41.0
43	34.10911621373199	36.0	33.0	40.0	24.0	41.0
44	34.074281143233726	36.0	33.0	39.0	24.0	41.0
45	33.773510258233465	35.0	33.0	39.0	23.0	41.0
46	33.55258753750368	35.0	33.0	39.0	23.0	40.0
47	33.45660845285375	35.0	32.0	39.0	23.0	40.0
48	33.39573108340126	35.0	32.0	39.0	23.0	40.0
49	33.247901527895806	35.0	32.0	39.0	22.0	40.0
50	33.07247532994572	35.0	32.0	39.0	21.0	40.0
51	31.2360477619188	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	8.0
11	9.0
12	12.0
13	17.0
14	21.0
15	53.0
16	89.0
17	193.0
18	434.0
19	754.0
20	1283.0
21	1993.0
22	2786.0
23	3556.0
24	4291.0
25	4940.0
26	5968.0
27	7152.0
28	8858.0
29	11063.0
30	13929.0
31	18034.0
32	23634.0
33	31797.0
34	51673.0
35	54864.0
36	45612.0
37	57581.0
38	66421.0
39	44252.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.653231820467905	35.16805119578225	19.27689426128579	25.901822722464058
2	21.264589583947554	35.50385008931514	20.07292624130695	23.15863408543036
3	21.005532335547425	32.08797974367423	22.014663290612372	24.89182463016597
4	21.353687934652537	33.44895163108513	17.516605678014603	27.680754756247723
5	17.40777995525572	37.312698357642084	18.7097865108132	26.569735176289
6	20.39506772341791	37.41805553146841	21.22816982015574	20.958706924957944
7	77.30181578536619	9.845476145054716	7.166672447581554	5.686035621997537
8	78.96801997884185	7.103804998179012	5.547727233311944	8.380447789667194
9	72.99127659943463	11.67578605990184	9.659908777163073	5.673028563500459
10	44.380950729262416	27.366417509235013	12.309229808709526	15.94340195279305
11	37.51213992126394	24.630599538682993	20.96607759143962	16.891182948613448
12	33.59528103917726	23.027262794609875	27.02346473352873	16.353991432684136
13	24.429423700594857	34.96860963216039	20.552669915540832	20.049296751703924
14	17.07111392448969	36.032587017221346	28.286666897903263	18.609632160385704
15	15.669820155737845	27.704601030159033	35.04448414006001	21.581094674043115
16	15.675239763444962	26.622413763202164	35.00307833717764	22.69926813617523
17	16.893784360312864	25.67289849291549	26.2636357329911	31.16968141378054
18	21.71680165102929	26.534399334038604	27.078744732141306	24.670054282790794
19	23.71121728724788	29.376441615650094	24.854754513449297	22.05758658365273
20	26.279244203187595	29.153153778116923	22.762135585577774	21.805466433117704
21	20.80392292884272	30.21496332009504	24.307590919338896	24.67352283172335
22	20.11411525988103	28.906670019597303	24.3667730355006	26.61244168502107
23	22.299517871698374	30.0857598723574	20.322011411525988	27.292710844418238
24	18.291176011515585	31.152989022042625	27.01305908673107	23.542775879710724
25	19.208173635559564	26.411482631241217	27.753594283831358	26.62674944936786
26	22.21128665822653	31.35438164443905	21.312715700386743	25.12161599694768
27	17.561263245521236	30.283683945821267	25.105790742442903	27.049262066214595
28	18.9580045437991	29.067740760652782	29.815213055618184	22.159041639929935
29	18.75661192140268	28.458360070064685	28.183911135776345	24.601116872756283
30	21.643094986212517	28.594067047050864	24.394738211269313	25.3680997554673
31	26.164998872721597	31.579403756438495	19.201670106311024	23.053927264528884
32	25.365064775151314	28.890411196475956	19.873267893376806	25.871256134995924
33	23.891365047432405	30.303411317875167	22.647890255111776	23.157333379580653
34	20.523187249614125	28.670375123567055	23.453243960389173	27.353193666429647
35	19.875869305076222	27.67360087407433	25.994389622101593	26.456140198747853
36	25.640380846672794	26.95517767641907	24.634284871923832	22.770156604984304
37	21.589549262066214	31.813964378002463	21.100267078267805	25.496219281663517
38	22.58220460970153	31.519571287351937	22.78966719272992	23.10855691021661
39	22.31902845944399	29.34999393003937	23.91109241948631	24.419885191030332
40	24.652711538128024	26.979457518946948	21.624668320008325	26.743162622916707
41	21.255267858691315	22.564211512113907	27.70893671632473	28.471583912870052
42	24.5345640901129	24.393871074036177	22.343525086280156	28.728039749570765
43	22.10853089609962	24.760886907962053	25.138308388685594	27.992273807252737
44	21.858145020030868	24.63710306793153	25.202476543937845	28.302275368099757
45	23.442838313591512	28.679913633131584	24.044414769081353	23.832833284195555
46	20.0510310261702	27.160472416364613	29.487001612875254	23.301494944589933
47	22.23079724597215	26.653413919286866	24.18424064792494	26.931548186816045
48	23.164920830370615	22.3118745772706	30.16228473318187	24.360919859176914
49	22.020733251244344	22.130209326928078	28.974957076706957	26.874100345120617
50	19.954345224675258	26.242390870779207	25.526352300515082	28.276911604030452
51	20.96282582681535	24.611522519553944	29.00834186018279	25.417309793447913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4133.0
1	3379.0
2	2625.0
3	1783.0
4	941.0
5	873.5
6	806.0
7	814.5
8	823.0
9	908.5
10	994.0
11	1053.5
12	1113.0
13	1193.0
14	1273.0
15	1284.0
16	1295.0
17	1293.5
18	1292.0
19	1257.5
20	1223.0
21	1196.5
22	1170.0
23	1422.5
24	1675.0
25	1562.0
26	1879.5
27	2310.0
28	3093.5
29	3877.0
30	4198.5
31	4520.0
32	5483.0
33	6446.0
34	7317.5
35	8189.0
36	8891.5
37	9594.0
38	10347.0
39	11100.0
40	13561.0
41	16022.0
42	21647.5
43	27273.0
44	36845.0
45	46417.0
46	45745.0
47	45073.0
48	45218.5
49	45364.0
50	42490.0
51	39616.0
52	35731.0
53	31846.0
54	29172.0
55	26498.0
56	23619.0
57	20740.0
58	20366.5
59	19993.0
60	20012.0
61	20031.0
62	17721.0
63	15411.0
64	13905.5
65	12400.0
66	10693.5
67	8987.0
68	7635.0
69	6283.0
70	5334.5
71	4386.0
72	3596.5
73	2807.0
74	2449.5
75	1569.0
76	1046.0
77	1051.0
78	1056.0
79	736.5
80	417.0
81	311.5
82	206.0
83	203.5
84	201.0
85	140.5
86	80.0
87	71.5
88	63.0
89	79.0
90	95.0
91	58.0
92	21.0
93	14.5
94	8.0
95	7.0
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.478142510517717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64663015375747	23.77312856665478
2	8.577554410465384	5.057007426068369
3	3.03389958355599	2.683011728599915
4	1.479802200865856	1.7448728065800592
5	0.7840627565148734	1.1556356836867396
6	0.4999729171371105	0.8842963741660208
7	0.33456774688837954	0.6903705005538932
8	0.27113482770793784	0.6394040872591409
9	0.21586218713329974	0.5726894683452709
>10	2.622685491316242	19.283662582526528
>50	1.175787247143761	25.045124885153385
>100	0.3469898454916738	12.964282297738597
>500	0.005893670411748175	1.1824801432431558
>1k	0.005156961610279653	4.324033449424146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	3774	0.8181439794661902	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	3687	0.7992837446454275	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3567	0.773269627651272	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGT	3353	0.7268777856783615	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	3156	0.6841712769462895	RNA PCR Primer, Index 13 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1340	0.29049097310140304	Illumina Single End Adapter 1 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	1034	0.22415497476630653	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	891	0.19315481868160456	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTT	780	0.16909176046201072	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	704	0.1526161530323789	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.15239936872409426	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	658	0.14264407485128597	No Hit
AACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	605	0.13115450651220062	RNA PCR Primer, Index 13 (95% over 23bp)
AATGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	569	0.12335027141395397	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	535	0.11597960493227658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.335686165692583E-4	0.0	0.0	1.0370961308336657	0.0
2	4.335686165692583E-4	0.0	0.0	1.2998387124746362	0.0
3	4.335686165692583E-4	0.0	0.0	1.770477445760566	0.0
4	4.335686165692583E-4	0.0	0.0	4.341539342016268	0.0
5	4.335686165692583E-4	0.0	0.0	4.791366781706873	0.0
6	4.335686165692583E-4	0.0	0.0	6.384297878982328	0.0
7	4.335686165692583E-4	0.0	0.0	7.616283102963875	0.0
8	4.335686165692583E-4	0.0	0.0	8.316279634414942	0.0
9	4.335686165692583E-4	0.0	0.0	10.142253863096373	0.0
10	4.335686165692583E-4	0.0	0.0	11.577799552557188	0.0
11	4.335686165692583E-4	0.0	0.0	14.091847175734031	0.0
12	4.335686165692583E-4	0.0	0.0	14.887662371446906	0.0
13	4.335686165692583E-4	0.0	0.0	15.368056398605644	0.0
14	4.335686165692583E-4	0.0	0.0	15.820485249995665	0.0
15	4.335686165692583E-4	0.0	0.0	16.09189920396802	0.0
16	4.335686165692583E-4	0.0	0.0	16.64166420977784	0.0
17	4.335686165692583E-4	0.0	0.0	17.204219489776452	0.0
18	4.335686165692583E-4	0.0	0.0	18.10842683963164	0.0
19	4.335686165692583E-4	0.0	0.0	18.41279200846326	0.0
20	4.335686165692583E-4	0.0	0.0	18.78370995993826	0.0
21	4.335686165692583E-4	0.0	0.0	19.107585716515494	0.0
22	4.335686165692583E-4	0.0	0.0	19.440566414040685	0.0
23	4.335686165692583E-4	0.0	0.0	19.796309463935764	0.0
24	4.335686165692583E-4	0.0	0.0	20.0425764381471	0.0
25	4.335686165692583E-4	0.0	0.0	20.260444667973154	0.0
26	4.335686165692583E-4	0.0	0.0	20.480047172265483	0.0
27	4.335686165692583E-4	0.0	0.0	20.70745391165606	0.0
28	4.335686165692583E-4	0.0	0.0	20.920336102391566	0.0
29	4.335686165692583E-4	0.0	0.0	21.133001508818786	0.0
30	4.335686165692583E-4	0.0	0.0	21.471185029742806	0.0
31	4.335686165692583E-4	0.0	0.0	21.70032604359966	0.0
32	4.335686165692583E-4	0.0	0.0	21.906487920778343	0.0
33	4.335686165692583E-4	0.0	0.0	22.15275489498968	0.0
34	4.335686165692583E-4	0.0	0.0	22.378644144222264	0.0
35	4.335686165692583E-4	0.0	0.0	22.64442170617922	0.0
36	4.335686165692583E-4	0.0	0.0	22.837142956244254	0.0
37	4.335686165692583E-4	0.0	0.0	23.03918593156553	0.0
38	6.503529248538874E-4	0.0	0.0	23.259655573091	0.0
39	6.503529248538874E-4	0.0	0.0	23.497034390662666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGC	20	7.028889E-4	45.000004	21
TAATACG	20	7.028889E-4	45.000004	1
GTCGAGT	20	7.028889E-4	45.000004	19
GGGTCTA	20	7.028889E-4	45.000004	7
TGGACGT	35	1.2098099E-7	45.000004	31
GGCGTAC	20	7.028889E-4	45.000004	43
GACCGAC	40	6.7993824E-9	45.000004	43
GCACGAC	20	7.028889E-4	45.000004	40
CGTCTCA	20	7.028889E-4	45.000004	28
GTCTAGC	20	7.028889E-4	45.000004	9
AGGCGTA	20	7.028889E-4	45.000004	23
CATTCTC	20	7.028889E-4	45.000004	28
GAATAAG	20	7.028889E-4	45.000004	17
CGACAGA	20	7.028889E-4	45.000004	37
TAACGAA	20	7.028889E-4	45.000004	18
TCGATGG	40	6.7993824E-9	45.000004	2
GCGTTCC	20	7.028889E-4	45.000004	37
TAGCCGT	35	1.2098099E-7	45.000004	44
AGAACCG	75	0.0	45.000004	1
CCCACTA	20	7.028889E-4	45.000004	26
>>END_MODULE