##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449318
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37389999955488	31.0	31.0	34.0	30.0	34.0
2	31.530065566035635	31.0	31.0	34.0	30.0	34.0
3	31.734622249720687	31.0	31.0	34.0	30.0	34.0
4	35.54939040946501	37.0	35.0	37.0	33.0	37.0
5	35.14968463315514	37.0	35.0	37.0	32.0	37.0
6	35.169948677773874	37.0	35.0	37.0	32.0	37.0
7	35.125975367111934	37.0	35.0	37.0	32.0	37.0
8	35.46146381849826	37.0	35.0	37.0	33.0	37.0
9	36.34813650910936	39.0	37.0	39.0	32.0	39.0
10	36.05836400945433	38.0	35.0	39.0	31.0	39.0
11	36.29084746215376	38.0	35.0	39.0	32.0	39.0
12	35.825718088302715	37.0	35.0	39.0	31.0	39.0
13	35.5415474118553	37.0	35.0	39.0	30.0	39.0
14	36.521730711878895	38.0	35.0	40.0	31.0	41.0
15	36.61056534570171	38.0	35.0	40.0	31.0	41.0
16	36.62053156116603	38.0	35.0	40.0	31.0	41.0
17	36.59961541714331	38.0	35.0	40.0	31.0	41.0
18	36.4209802411655	38.0	35.0	40.0	31.0	41.0
19	36.428972353655986	38.0	35.0	40.0	31.0	41.0
20	36.43548889650537	38.0	35.0	40.0	31.0	41.0
21	36.489989272630964	38.0	35.0	40.0	31.0	41.0
22	36.45900453576309	38.0	35.0	40.0	31.0	41.0
23	36.39784517869304	38.0	35.0	40.0	31.0	41.0
24	36.07253659991365	38.0	34.0	40.0	30.0	41.0
25	36.1535438152934	38.0	35.0	40.0	30.0	41.0
26	36.044912511851294	38.0	35.0	40.0	30.0	41.0
27	35.909286963798465	38.0	34.0	40.0	30.0	41.0
28	35.86396494242385	38.0	34.0	40.0	30.0	41.0
29	35.65451640041129	38.0	34.0	40.0	29.0	41.0
30	35.514862524982306	37.0	34.0	40.0	29.0	41.0
31	35.36084243230852	37.0	34.0	40.0	28.0	41.0
32	35.28907588834634	37.0	34.0	40.0	28.0	41.0
33	35.251901771128686	37.0	34.0	40.0	28.0	41.0
34	35.214068877721346	37.0	34.0	40.0	27.0	41.0
35	34.98124936014137	37.0	34.0	40.0	27.0	41.0
36	34.849115771012954	37.0	33.0	40.0	26.0	41.0
37	34.5435437707815	37.0	33.0	40.0	25.0	41.0
38	34.57925344633422	37.0	33.0	40.0	25.0	41.0
39	34.6521750742236	37.0	33.0	40.0	25.0	41.0
40	34.49968173988133	37.0	33.0	40.0	24.0	41.0
41	34.60468977428013	37.0	33.0	40.0	25.0	41.0
42	34.51352271665057	36.0	33.0	40.0	25.0	41.0
43	34.41911741795343	36.0	33.0	40.0	25.0	41.0
44	34.193735839650316	36.0	33.0	40.0	24.0	41.0
45	33.92046612866611	36.0	33.0	39.0	23.0	41.0
46	33.842289870425844	35.0	33.0	39.0	23.0	41.0
47	33.71558895926716	35.0	33.0	39.0	23.0	41.0
48	33.68154848014101	35.0	33.0	39.0	23.0	41.0
49	33.52966718448849	35.0	32.0	39.0	23.0	40.0
50	33.242725641972946	35.0	32.0	39.0	22.0	40.0
51	31.163683627186092	34.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	7.0
13	6.0
14	19.0
15	54.0
16	79.0
17	194.0
18	394.0
19	719.0
20	1204.0
21	1804.0
22	2444.0
23	3256.0
24	3955.0
25	4637.0
26	5429.0
27	6770.0
28	8295.0
29	10385.0
30	13168.0
31	16844.0
32	21714.0
33	29649.0
34	48892.0
35	50982.0
36	43469.0
37	55712.0
38	69038.0
39	50190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.252471523508962	35.832750969246725	19.278105929430826	24.636671577813487
2	21.08328622490085	36.50933192082223	20.304773011541936	22.102608842734988
3	20.324580809137405	31.229329784250798	23.935831638171628	24.51025776844017
4	22.797439675241144	31.149208355774753	18.740402120547138	27.31294984843696
5	17.089900693940592	37.18658055096836	18.220725633070565	27.502793122020485
6	20.576740749313405	35.93980210007167	22.598916580239383	20.88454057037555
7	76.38354127811483	10.446721475658665	7.489573086321936	5.6801641599045665
8	78.6353985373388	6.253477492555384	5.7333558860317195	9.377768084074086
9	73.14285205578231	10.72447576104229	10.255987963980967	5.8766842191944235
10	42.78528792525561	27.23416377710219	13.463515817305339	16.517032480336866
11	35.20023680333305	24.997218005955695	22.628294437347268	17.17425075336399
12	33.29713031750342	23.068739734441976	28.178483835501805	15.4556461125528
13	24.849883601369186	34.466012935159505	21.6737366408646	19.010366822606706
14	17.37922807454854	35.216038529504715	30.195985916433347	17.208747479513395
15	16.242839147329953	28.778504310977972	34.29286162584183	20.685794915850245
16	17.13552539626723	25.8845628263279	35.06848156539466	21.911430212010202
17	16.93633462269484	26.823318896638902	26.88941907513164	29.35092740553461
18	20.823114141877245	27.111756039152674	27.600051633809464	24.46507818516062
19	23.007980984514308	30.74704329672971	26.615225742124732	19.62974997663125
20	25.353090684103464	29.276369965147182	24.470864732772778	20.899674617976576
21	20.86673580849198	29.59974895285744	25.133201874841426	24.40031336380915
22	20.42517771377955	29.618889071882275	25.479504493476778	24.476428720861396
23	21.889174259655743	29.40634472689721	21.642355748044814	27.06212526540223
24	18.419916406642955	31.416502343551784	27.12622240818307	23.03735884162219
25	20.32880944008475	27.2047859199943	26.39823020666877	26.06817443325217
26	23.13550759150535	31.87341704538879	22.128648306989703	22.862427056116157
27	18.655829501600202	28.9699055012263	28.07121014515332	24.30305485202017
28	23.138178305787882	28.68992562060723	27.900729550118182	20.271166523486706
29	23.03646860352801	29.222733119972936	27.08860984870404	20.65218842779501
30	23.99124895953423	26.643268242091345	26.313657587721835	23.051825210652588
31	29.48913686965579	30.48019442799977	19.72367009556706	20.306998606777384
32	29.80850978594225	26.95440645600666	21.706230331302105	21.530853426748983
33	26.639707289714632	27.6496824075599	21.97129872384369	23.73931157888177
34	24.15304973315113	26.762115027664144	25.954891635767986	23.129943603416734
35	25.478169136335516	27.168286158132993	25.175043065267804	22.17850164026369
36	26.973101455984406	25.82602967163568	28.62716383496766	18.573705037412257
37	22.80567437761229	29.771342345510305	25.948437409585196	21.474545867292207
38	24.560778780284785	30.14680026173	23.56638282908764	21.726038128897574
39	24.490672530368247	25.53981812435736	27.34700145553928	22.62250788973511
40	23.05627640112348	28.51566151367183	23.079422591572115	25.348639493632575
41	19.69451479798272	22.843732056138414	31.723189366996202	25.73856377888266
42	27.045210741612845	26.497714313693198	22.908941996536974	23.548132948156987
43	23.457106103027257	23.911350090581728	27.227264431872307	25.404279374518712
44	22.9140608655785	25.099150267739105	25.209984910464307	26.776803956218092
45	25.192402708104282	28.26528204968419	23.58685830525374	22.955456936957788
46	21.489902474416784	25.721426695569733	31.019011034501176	21.76965979551231
47	22.6209499730703	27.011826813081157	24.466413542301886	25.900809671546654
48	22.89848169893038	22.324055568661837	32.49903186607258	22.278430866335203
49	23.04114235352245	21.923670985805156	29.380082703118948	25.65510395755345
50	19.9700434881309	24.703216875353313	26.39355645667434	28.93318317984145
51	20.203953547376248	24.91932217271509	30.068681868965857	24.808042410942807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3279.0
1	2588.0
2	1897.0
3	1286.5
4	676.0
5	663.0
6	650.0
7	683.5
8	717.0
9	713.5
10	710.0
11	776.0
12	842.0
13	900.5
14	959.0
15	1030.5
16	1102.0
17	1148.0
18	1194.0
19	1190.0
20	1186.0
21	1347.0
22	1508.0
23	1741.0
24	1974.0
25	1973.5
26	2377.5
27	2782.0
28	3099.0
29	3416.0
30	3832.0
31	4248.0
32	4964.5
33	5681.0
34	6846.5
35	8012.0
36	8998.5
37	9985.0
38	10986.0
39	11987.0
40	14654.5
41	17322.0
42	22572.5
43	27823.0
44	39962.5
45	52102.0
46	51483.0
47	50864.0
48	47767.0
49	44670.0
50	42951.5
51	41233.0
52	36706.5
53	32180.0
54	27756.0
55	23332.0
56	21775.5
57	20219.0
58	18835.0
59	17451.0
60	16426.0
61	15401.0
62	14118.0
63	12835.0
64	11102.5
65	9370.0
66	7744.0
67	6118.0
68	5286.5
69	4455.0
70	3984.5
71	3514.0
72	2785.0
73	2056.0
74	1748.5
75	1117.0
76	793.0
77	599.5
78	406.0
79	429.0
80	452.0
81	340.0
82	228.0
83	169.5
84	111.0
85	100.5
86	90.0
87	67.0
88	44.0
89	31.0
90	18.0
91	12.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.458931436577224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52558913295773	22.632132885882548
2	8.956485079027141	5.0978398955352064
3	3.1062272898387304	2.651997284038365
4	1.432885847444971	1.6311360035551312
5	0.852153248118431	1.2125685430829505
6	0.5303100041121672	0.9055233628295491
7	0.3794733986503467	0.7559585203936607
8	0.29512513056824485	0.6719156684842065
9	0.24671371340263415	0.6319087788770036
>10	3.171343001374555	22.31618241338915
>50	1.2265334977816353	24.8980127480415
>100	0.26623037730333393	10.1024723235106
>500	0.005465139710039113	1.1171643978756773
>1k	0.003903671221456509	3.015766183843789
>5k	0.0015614684885826037	2.359420990660664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	5321	1.1842392247806677	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	5298	1.1791203557391425	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGT	4493	0.9999599392857619	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	3862	0.8595248799291371	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2836	0.6311788087724062	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	1269	0.2824280353780619	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	1113	0.24770874970510862	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	988	0.21988880926203713	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	875	0.1947395831015005	No Hit
AACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	804	0.17893785692983588	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669	0.14889232125131865	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	604	0.1344259522209215	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTAT	573	0.12752660699103974	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	515	0.11461815462545458	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	477	0.10616089273076085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.22559523544572E-4	0.0	0.0	1.2084982128470259	0.0
2	2.22559523544572E-4	0.0	0.0	1.5102889267734656	0.0
3	2.22559523544572E-4	0.0	0.0	2.0566725570753897	0.0
4	2.22559523544572E-4	0.0	0.0	5.149804815297851	0.0
5	2.22559523544572E-4	0.0	0.0	5.5446254100659225	0.0
6	2.22559523544572E-4	0.0	0.0	7.212486479508945	0.0
7	2.22559523544572E-4	0.0	0.0	8.602815823091886	0.0
8	2.22559523544572E-4	0.0	0.0	9.423170226877179	0.0
9	2.22559523544572E-4	0.0	0.0	11.67903355752496	0.0
10	2.22559523544572E-4	0.0	0.0	13.420561829261237	0.0
11	2.22559523544572E-4	0.0	0.0	16.375262063838974	0.0
12	2.22559523544572E-4	0.0	0.0	17.262161765164095	0.0
13	2.22559523544572E-4	0.0	0.0	17.688363252751948	0.0
14	2.22559523544572E-4	0.0	0.0	18.188677061680146	0.0
15	2.22559523544572E-4	0.0	0.0	18.489800097035953	0.0
16	2.22559523544572E-4	0.0	0.0	19.072906048722732	0.0
17	2.22559523544572E-4	0.0	0.0	19.693402000364998	0.0
18	2.22559523544572E-4	0.0	0.0	20.700038725357096	0.0
19	2.22559523544572E-4	0.0	0.0	21.04033223685675	0.0
20	2.22559523544572E-4	0.0	0.0	21.43515283162482	0.0
21	2.22559523544572E-4	0.0	0.0	21.830195985916433	0.0
22	2.22559523544572E-4	0.0	0.0	22.201870390235868	0.0
23	2.22559523544572E-4	0.0	0.0	22.592017235009504	0.0
24	2.22559523544572E-4	0.0	0.0	22.851076520415386	0.0
25	4.45119047089144E-4	0.0	0.0	23.083651222519464	0.0
26	4.45119047089144E-4	0.0	0.0	23.30109187702251	0.0
27	4.45119047089144E-4	0.0	0.0	23.559483483857758	0.0
28	4.45119047089144E-4	0.0	0.0	23.785603959779042	0.0
29	4.45119047089144E-4	0.0	0.0	24.02240729283047	0.0
30	4.45119047089144E-4	0.0	0.0	24.40409687570941	0.0
31	6.67678570633716E-4	0.0	0.0	24.664268958733015	0.0
32	6.67678570633716E-4	0.0	0.0	24.896621101313546	0.0
33	6.67678570633716E-4	0.0	0.0	25.14366217244802	0.0
34	6.67678570633716E-4	0.0	0.0	25.383803898352614	0.0
35	6.67678570633716E-4	0.0	0.0	25.670905683725113	0.0
36	6.67678570633716E-4	0.0	0.0	25.897916397740577	0.0
37	6.67678570633716E-4	0.0	0.0	26.127597826038574	0.0
38	6.67678570633716E-4	0.0	0.0	26.362620682901643	0.0
39	6.67678570633716E-4	0.0	0.0	26.600314254047245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.1625383E-6	45.000004	35
CGGGTAT	35	1.2097189E-7	45.000004	6
CAACGAA	35	1.2097189E-7	45.000004	5
TAACGTT	30	2.1625383E-6	45.000004	34
CGATTGG	45	3.8380676E-10	45.000004	2
ACGGGTA	75	0.0	45.000004	5
CGTTACT	30	2.1625383E-6	45.000004	27
CCATAGG	45	3.8380676E-10	45.000004	2
AGCGCGT	30	2.1625383E-6	45.000004	22
CGAATAT	45	3.8380676E-10	45.000004	13
GACGGGT	30	2.1625383E-6	45.000004	4
TTAACCG	30	2.1625383E-6	45.000004	1
TCGTAAG	30	2.1625383E-6	45.000004	1
TCCAACG	30	2.1625383E-6	45.000004	1
CGAGCGT	30	2.1625383E-6	45.000004	40
ACCACTA	30	2.1625383E-6	45.000004	10
ACGTAGG	35	1.2097189E-7	45.000004	2
CGCACGG	35	1.2097189E-7	45.000004	2
CGCGTTA	30	2.1625383E-6	45.000004	25
ACTTTCG	30	2.1625383E-6	45.000004	28
>>END_MODULE