SRR3548768_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614394
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	5045	0.8211343209731867	TruSeq Adapter, Index 23 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	4264	0.6940171941783285	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	3974	0.6468162123979075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3432	0.558599205070362	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGT	3215	0.5232798497381159	No Hit
AAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1688	0.2747422663632783	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	1190	0.1936867873058656	No Hit
AATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	994	0.1617854341025466	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	864	0.1406263733044268	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	780	0.12695436478871863	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	756	0.12304807664137345	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	740	0.12044388454314331	No Hit
AACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG	720	0.11718864442035568	TruSeq Adapter, Index 22 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	657	0.10693463803357454	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	655	0.10660911402129579	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAATT	35	1.2107012E-7	45.000004	21
CTACGCG	35	1.2107012E-7	45.000004	1
CATACGA	135	0.0	45.000004	18
TACAACG	55	1.8189894E-12	45.000004	1
CCACACG	35	1.2107012E-7	45.000004	1
CTTCGTG	25	3.888678E-5	45.0	32
TACCCTA	20	7.03072E-4	45.0	17
CAAACGA	20	7.03072E-4	45.0	36
GGTAATC	45	3.8380676E-10	45.0	8
CGAGTTA	25	3.888678E-5	45.0	26
ACGTTAG	25	3.888678E-5	45.0	1
CGTGCGA	40	6.8066583E-9	45.0	44
CCGATAG	20	7.03072E-4	45.0	1
CAGCCGA	20	7.03072E-4	45.0	15
GCAACTA	25	3.888678E-5	45.0	9
TTTCGTG	50	2.1827873E-11	45.0	16
GACGTGA	20	7.03072E-4	45.0	9
GACCGTC	25	3.888678E-5	45.0	9
CGCATCG	40	6.8066583E-9	45.0	21
GAGTACA	45	3.8380676E-10	45.0	9