##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548768_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614394
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.381167459317638	31.0	31.0	34.0	30.0	34.0
2	31.541715902173525	31.0	31.0	34.0	30.0	34.0
3	31.718125502527695	31.0	31.0	34.0	30.0	34.0
4	35.55907772536841	37.0	35.0	37.0	33.0	37.0
5	35.16509113044724	37.0	35.0	37.0	32.0	37.0
6	35.17322434789402	37.0	35.0	37.0	32.0	37.0
7	35.17596200483729	37.0	35.0	37.0	32.0	37.0
8	35.498193341731856	37.0	35.0	37.0	33.0	37.0
9	36.31741846437303	39.0	37.0	39.0	32.0	39.0
10	35.89195044222437	38.0	35.0	39.0	30.0	39.0
11	36.26685807478589	38.0	35.0	39.0	32.0	39.0
12	35.96088340706452	37.0	35.0	39.0	32.0	39.0
13	35.719565946282025	37.0	35.0	39.0	30.0	39.0
14	36.69730986956253	38.0	35.0	40.0	31.0	41.0
15	36.79233683922695	38.0	35.0	40.0	31.0	41.0
16	36.79388307828527	38.0	35.0	40.0	32.0	41.0
17	36.75554123249901	38.0	35.0	40.0	32.0	41.0
18	36.545252720566936	38.0	35.0	40.0	31.0	41.0
19	36.61448516749838	38.0	35.0	40.0	31.0	41.0
20	36.54577193136652	38.0	35.0	40.0	31.0	41.0
21	36.60716250484217	38.0	35.0	40.0	31.0	41.0
22	36.50838061569612	38.0	35.0	40.0	31.0	41.0
23	36.492535734398444	38.0	35.0	40.0	31.0	41.0
24	36.161459910090265	38.0	35.0	40.0	30.0	41.0
25	36.24958251545425	38.0	35.0	40.0	30.0	41.0
26	36.10135841170324	38.0	35.0	40.0	30.0	41.0
27	36.03858273355534	38.0	35.0	40.0	30.0	41.0
28	35.79342734466808	38.0	34.0	40.0	29.0	41.0
29	35.30558566652669	38.0	34.0	40.0	27.0	41.0
30	35.33146319788279	38.0	34.0	40.0	27.0	41.0
31	35.15023095928671	38.0	34.0	40.0	27.0	41.0
32	35.22632708001706	38.0	34.0	40.0	27.0	41.0
33	35.16260575461349	37.0	34.0	40.0	27.0	41.0
34	35.12255328014271	37.0	34.0	40.0	27.0	41.0
35	35.02623398014955	37.0	34.0	40.0	26.0	41.0
36	34.79351198091127	37.0	34.0	40.0	25.0	41.0
37	34.73645575965911	37.0	33.0	40.0	25.0	41.0
38	34.653329947883606	37.0	33.0	40.0	25.0	41.0
39	34.577578882606275	37.0	33.0	40.0	25.0	41.0
40	34.42032637037471	37.0	33.0	40.0	24.0	41.0
41	34.45201287772993	37.0	33.0	40.0	24.0	41.0
42	34.40432686517121	37.0	33.0	40.0	24.0	41.0
43	34.18632994462837	37.0	33.0	40.0	23.0	41.0
44	34.10917424323805	36.0	33.0	40.0	23.0	41.0
45	33.93760030208628	36.0	33.0	40.0	23.0	41.0
46	33.81708968512062	36.0	33.0	40.0	23.0	41.0
47	33.751011565868154	36.0	33.0	40.0	23.0	41.0
48	33.78555617405118	36.0	33.0	39.0	23.0	41.0
49	33.44990999261061	36.0	32.0	39.0	22.0	41.0
50	33.14310035579774	35.0	32.0	39.0	20.0	40.0
51	31.21304244507596	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	14.0
11	12.0
12	11.0
13	13.0
14	22.0
15	58.0
16	142.0
17	278.0
18	536.0
19	1068.0
20	1785.0
21	2803.0
22	3797.0
23	4929.0
24	6084.0
25	6993.0
26	7845.0
27	9657.0
28	11548.0
29	14268.0
30	17819.0
31	22697.0
32	28690.0
33	38086.0
34	60800.0
35	66088.0
36	59051.0
37	78472.0
38	98116.0
39	72703.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.256210184344248	34.739434304371464	20.07115954908414	25.933195962200152
2	22.67111983515464	33.81201639338926	21.10404724004466	22.41281653141144
3	20.689655172413794	31.98371077842557	22.08501385104672	25.241620198113914
4	21.653857296783496	31.7602385439962	19.512723106019916	27.07318105320039
5	18.238947645973106	36.17141443438575	18.198582668450538	27.391055251190604
6	20.290725495366164	37.26631444968538	20.760619407090562	21.682340647857888
7	79.72880594537057	8.222736550161622	6.851466648437323	5.196990856030495
8	81.71710661236926	6.26389580627415	5.254445844197697	6.764551737158891
9	74.86368681985826	11.076280041797283	9.105069385443217	4.954963752901233
10	38.7323118389828	34.803074248771956	12.186967971692432	14.277645940552805
11	31.961086859572198	26.634700208660895	23.714261532501947	17.689951399264967
12	31.31687483927252	23.349674638749725	29.21952362816043	16.113926893817325
13	25.900318036959995	32.1280806778712	22.572811583446452	19.398789701722347
14	17.29639286841994	35.60679303508824	29.170532264312477	17.92628183217935
15	15.81949042471118	29.163207974036204	34.138679739710994	20.878621861541617
16	17.601083343912862	27.80007617261887	32.4070547563941	22.191785727074155
17	17.93702412458455	25.33504558963792	26.834246428187775	29.893683857589753
18	23.579820115430813	26.237886437693074	25.70353877153748	24.478754675338628
19	24.928303336295603	29.32906245829224	25.67359056240784	20.069043643004324
20	24.41446368291357	29.284302906603905	23.912505655979714	22.38872775450281
21	23.64476215588043	29.231730778620886	24.63874972737364	22.484757338125046
22	19.468777364362282	30.01591812420043	24.286532746088017	26.228771765349272
23	20.966676106863023	27.8249787595582	22.49452305849341	28.71382207508537
24	17.452481632307606	30.083301594742135	27.25759040615631	25.206626366793945
25	19.459337168006197	27.21299361647412	25.2349469558622	28.09272225965748
26	20.921753793168556	32.04132852859891	21.81222472875712	25.22469294947542
27	17.93441993248632	29.06538800834644	26.335706403382847	26.664485655784397
28	21.620328323518784	27.44037213905084	27.433373372786846	23.505926164643533
29	20.197951151866718	29.616337399128245	27.483178546665492	22.70253290233954
30	22.864481098448227	26.89642151453302	27.80007617261887	22.43902121439988
31	26.609797621721565	31.048317529142537	21.09411875767016	21.247766091465735
32	27.357037991907475	28.42345465613271	22.757546460414652	21.461960891545164
33	24.62475219484565	25.88599498041973	27.551050303225615	21.938202521508998
34	22.829975553146678	26.72373102601913	26.198498032207347	24.24779538862684
35	23.497950826342706	26.23935129574833	27.30723281802882	22.955465059880144
36	24.597245415808096	26.776140391996016	29.606897202772164	19.019716989423724
37	23.856190001855488	29.55432507478914	25.3890825756762	21.200402347679177
38	25.9577730251272	26.70322301324557	25.649501785499208	21.689502176128023
39	25.554611535919946	25.096436488637586	26.71477911568147	22.634172859761
40	26.17880382946448	24.307366282873858	25.684007330800757	23.829822556860908
41	20.88122605363985	22.19927277935657	29.934862645143017	26.984638521860564
42	27.16302568058933	24.119864451801288	24.544998811837353	24.17211105577203
43	22.242892997001924	26.08098386377471	26.781674300204756	24.894448839018608
44	21.85047380019987	23.929921190636627	25.942961682568516	28.276643326594986
45	23.42275477950631	26.647395645139767	25.350019694202743	24.579829881151184
46	20.742227300396817	26.61109971777068	29.875617274908283	22.77105570692422
47	21.08793380143686	25.023519109887143	27.024189689352436	26.864357399323563
48	22.221082888179247	21.88416553547072	32.154122598853505	23.740628977496524
49	24.721595588498584	20.630247040172918	29.342246180789527	25.30591119053897
50	20.613157029528285	23.98949208488364	28.063587860558535	27.33376302502954
51	21.72856505760147	23.192446540819084	29.938443409278086	25.140544992301354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3996.0
1	3163.0
2	2330.0
3	1600.5
4	871.0
5	793.5
6	716.0
7	727.5
8	739.0
9	809.5
10	880.0
11	935.5
12	991.0
13	1099.5
14	1208.0
15	1228.0
16	1248.0
17	1340.5
18	1433.0
19	1453.0
20	1473.0
21	1698.0
22	1923.0
23	1864.0
24	1805.0
25	2133.5
26	3330.5
27	4199.0
28	5267.0
29	6335.0
30	6856.0
31	7377.0
32	8664.5
33	9952.0
34	11487.0
35	13022.0
36	14504.0
37	15986.0
38	16701.0
39	17416.0
40	21054.0
41	24692.0
42	32142.5
43	39593.0
44	49928.0
45	60263.0
46	61730.0
47	63197.0
48	61961.5
49	60726.0
50	55615.0
51	50504.0
52	47351.5
53	44199.0
54	39466.0
55	34733.0
56	31151.5
57	27570.0
58	27305.5
59	27041.0
60	26049.0
61	25057.0
62	22026.5
63	18996.0
64	16116.0
65	13236.0
66	11238.5
67	9241.0
68	7930.0
69	6619.0
70	5500.5
71	4382.0
72	3526.5
73	2671.0
74	2223.5
75	1634.5
76	1493.0
77	1086.0
78	679.0
79	638.0
80	597.0
81	473.0
82	349.0
83	279.5
84	210.0
85	153.0
86	96.0
87	77.0
88	58.0
89	43.0
90	28.0
91	19.5
92	11.0
93	10.5
94	10.0
95	5.0
96	0.0
97	2.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	614394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.15031106971161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.01148319191849	24.123711073853446
2	9.270589013037451	5.5902228508506
3	3.3153144684609615	2.998732875540408
4	1.3926035894386268	1.679497256734862
5	0.7732871756052112	1.1657424445357913
6	0.5029124136900924	0.9097779428145467
7	0.3206595347021344	0.6767589303116834
8	0.2537168306071369	0.6119713093141205
9	0.19941787524757737	0.5411259874417861
>10	2.498577860698602	18.797067602562223
>50	1.0866551134952638	23.59065141222682
>100	0.3661672334870063	14.515400678312709
>500	0.004846331031445671	1.0963749723804412
>1k	0.003230887354297114	2.8838899206861806
>5k	5.384812257161857E-4	0.8190747424343737
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	5045	0.8211343209731867	TruSeq Adapter, Index 23 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	4264	0.6940171941783285	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	3974	0.6468162123979075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3432	0.558599205070362	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGT	3215	0.5232798497381159	No Hit
AAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1688	0.2747422663632783	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	1190	0.1936867873058656	No Hit
AATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	994	0.1617854341025466	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	864	0.1406263733044268	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	780	0.12695436478871863	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	756	0.12304807664137345	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	740	0.12044388454314331	No Hit
AACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG	720	0.11718864442035568	TruSeq Adapter, Index 22 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	657	0.10693463803357454	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	655	0.10660911402129579	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1215929843064874	0.0
2	0.0	0.0	0.0	1.3592255132699864	0.0
3	0.0	0.0	0.0	1.7924979736130235	0.0
4	1.6276200613938286E-4	0.0	0.0	4.143106866277991	0.0
5	1.6276200613938286E-4	0.0	0.0	4.613651826026947	0.0
6	1.6276200613938286E-4	0.0	0.0	6.032610995550087	0.0
7	1.6276200613938286E-4	0.0	0.0	7.176665136703809	0.0
8	1.6276200613938286E-4	0.0	0.0	7.789789613830864	0.0
9	1.6276200613938286E-4	0.0	0.0	9.25725186118354	0.0
10	1.6276200613938286E-4	0.0	0.0	10.635032243153416	0.0
11	1.6276200613938286E-4	0.0	0.0	12.752565943026788	0.0
12	1.6276200613938286E-4	0.0	0.0	13.588837130570937	0.0
13	1.6276200613938286E-4	0.0	0.0	14.137833377279074	0.0
14	1.6276200613938286E-4	0.0	0.0	14.632792637948938	0.0
15	1.6276200613938286E-4	0.0	0.0	14.929833299153312	0.0
16	1.6276200613938286E-4	0.0	0.0	15.461251249198398	0.0
17	1.6276200613938286E-4	0.0	0.0	16.10399841144282	0.0
18	1.6276200613938286E-4	0.0	0.0	17.050296715137193	0.0
19	3.2552401227876573E-4	0.0	0.0	17.391608642011477	0.0
20	3.2552401227876573E-4	0.0	0.0	17.79167765310208	0.0
21	3.2552401227876573E-4	0.0	0.0	18.169773793363866	0.0
22	3.2552401227876573E-4	0.0	0.0	18.57814366676758	0.0
23	3.2552401227876573E-4	0.0	0.0	18.996767546558072	0.0
24	3.2552401227876573E-4	0.0	0.0	19.295110303811562	0.0
25	3.2552401227876573E-4	0.0	0.0	19.538439502989938	0.0
26	3.2552401227876573E-4	0.0	0.0	19.766794597603493	0.0
27	3.2552401227876573E-4	0.0	0.0	20.024935139340553	0.0
28	3.2552401227876573E-4	0.0	0.0	20.279657678948688	0.0
29	3.2552401227876573E-4	0.0	0.0	20.540890698802396	0.0
30	3.2552401227876573E-4	0.0	0.0	20.884644055768774	0.0
31	3.2552401227876573E-4	0.0	0.0	21.16736166043288	0.0
32	3.2552401227876573E-4	0.0	0.0	21.413457813715628	0.0
33	3.2552401227876573E-4	0.0	0.0	21.702034850600754	0.0
34	3.2552401227876573E-4	0.0	0.0	22.00672532609368	0.0
35	3.2552401227876573E-4	0.0	0.0	22.334853530470674	0.0
36	3.2552401227876573E-4	0.0	0.0	22.585507019925323	0.0
37	3.2552401227876573E-4	0.0	0.0	22.828510695091424	0.0
38	3.2552401227876573E-4	0.0	0.0	23.087465046859183	0.0
39	3.2552401227876573E-4	0.0	0.0	23.39606181049945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAATT	35	1.2107012E-7	45.000004	21
CTACGCG	35	1.2107012E-7	45.000004	1
CATACGA	135	0.0	45.000004	18
TACAACG	55	1.8189894E-12	45.000004	1
CCACACG	35	1.2107012E-7	45.000004	1
CTTCGTG	25	3.888678E-5	45.0	32
TACCCTA	20	7.03072E-4	45.0	17
CAAACGA	20	7.03072E-4	45.0	36
GGTAATC	45	3.8380676E-10	45.0	8
CGAGTTA	25	3.888678E-5	45.0	26
ACGTTAG	25	3.888678E-5	45.0	1
CGTGCGA	40	6.8066583E-9	45.0	44
CCGATAG	20	7.03072E-4	45.0	1
CAGCCGA	20	7.03072E-4	45.0	15
GCAACTA	25	3.888678E-5	45.0	9
TTTCGTG	50	2.1827873E-11	45.0	16
GACGTGA	20	7.03072E-4	45.0	9
GACCGTC	25	3.888678E-5	45.0	9
CGCATCG	40	6.8066583E-9	45.0	21
GAGTACA	45	3.8380676E-10	45.0	9
>>END_MODULE