##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548767_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629180
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16855430878286	31.0	31.0	34.0	28.0	34.0
2	31.362813503289996	31.0	31.0	34.0	28.0	34.0
3	31.327902984837408	31.0	31.0	34.0	28.0	34.0
4	35.34915604437522	37.0	35.0	37.0	33.0	37.0
5	35.021063924473125	37.0	35.0	37.0	32.0	37.0
6	35.08625512571919	36.0	35.0	37.0	32.0	37.0
7	35.048583871070285	37.0	35.0	37.0	32.0	37.0
8	35.419808321942845	37.0	35.0	37.0	33.0	37.0
9	35.82296640071204	39.0	37.0	39.0	32.0	39.0
10	35.56815219809911	37.0	35.0	39.0	30.0	39.0
11	36.099089290822974	38.0	35.0	39.0	31.0	39.0
12	35.84973457516132	37.0	35.0	39.0	31.0	39.0
13	35.602576369242506	37.0	35.0	39.0	30.0	39.0
14	36.573091960965066	38.0	35.0	40.0	31.0	41.0
15	36.69108681140532	38.0	35.0	40.0	31.0	41.0
16	36.705820274007436	38.0	35.0	40.0	31.0	41.0
17	36.68231348739629	38.0	35.0	40.0	31.0	41.0
18	36.48843574175911	38.0	35.0	40.0	31.0	41.0
19	36.55983661273403	38.0	35.0	40.0	31.0	41.0
20	36.47534727740869	38.0	35.0	40.0	31.0	41.0
21	36.5109253313837	38.0	35.0	40.0	31.0	41.0
22	36.43694968053657	38.0	35.0	40.0	31.0	41.0
23	36.43745351091897	38.0	35.0	40.0	31.0	41.0
24	36.05257001176134	38.0	34.0	40.0	30.0	41.0
25	36.177758352140884	38.0	35.0	40.0	30.0	41.0
26	36.03038399186242	38.0	35.0	40.0	30.0	41.0
27	35.95563908579421	38.0	34.0	40.0	30.0	41.0
28	35.70680727295846	38.0	34.0	40.0	29.0	41.0
29	35.24873327187768	38.0	34.0	40.0	27.0	41.0
30	35.30311198703074	38.0	34.0	40.0	27.0	41.0
31	35.19928160462825	37.0	34.0	40.0	27.0	41.0
32	35.256627674115514	38.0	34.0	40.0	27.0	41.0
33	35.1303696875298	37.0	34.0	40.0	27.0	41.0
34	35.164679424012206	37.0	34.0	40.0	27.0	41.0
35	35.045208048571155	37.0	34.0	40.0	27.0	41.0
36	34.84799898280301	37.0	34.0	40.0	26.0	41.0
37	34.76771671063924	37.0	33.0	40.0	25.0	41.0
38	34.705771003528405	37.0	33.0	40.0	25.0	41.0
39	34.55469341047077	37.0	33.0	40.0	25.0	41.0
40	34.37759941511173	37.0	33.0	40.0	24.0	41.0
41	34.41508789217712	37.0	33.0	40.0	24.0	41.0
42	34.41399122667599	37.0	33.0	40.0	24.0	41.0
43	34.23803680981595	37.0	33.0	40.0	24.0	41.0
44	34.265801519438	37.0	33.0	40.0	24.0	41.0
45	34.12927461139896	36.0	33.0	40.0	24.0	41.0
46	33.89957404876188	36.0	33.0	40.0	23.0	41.0
47	33.80604278584825	36.0	33.0	39.0	23.0	41.0
48	33.767510092501354	36.0	33.0	39.0	23.0	41.0
49	33.45498426523412	36.0	32.0	39.0	22.0	41.0
50	33.250112845290694	35.0	32.0	39.0	20.0	40.0
51	31.449384913697195	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	5.0
11	11.0
12	11.0
13	18.0
14	21.0
15	70.0
16	134.0
17	256.0
18	590.0
19	1088.0
20	1759.0
21	2810.0
22	3906.0
23	4915.0
24	5978.0
25	6899.0
26	8376.0
27	9986.0
28	11800.0
29	14938.0
30	18680.0
31	23620.0
32	30755.0
33	40696.0
34	63450.0
35	67970.0
36	62208.0
37	81214.0
38	98726.0
39	68279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.328808925903555	34.631266092374204	20.168473250897996	25.871451730824248
2	22.747862296957948	33.62201595727773	21.237165834896214	22.392955910868114
3	21.097778060332494	31.931879589306718	21.771671063924472	25.198671286436316
4	21.597158205918817	31.802822721637686	19.431164372675546	27.16885469976795
5	18.369465017959886	36.20363012174577	18.082901554404145	27.344003305890208
6	20.203121523252488	37.42410756858133	20.59124574843447	21.781525159731714
7	80.12142789026987	8.182872945738898	6.5752248958962465	5.120474268094981
8	82.09542579230109	6.32489907498649	5.141930767030103	6.437744365682317
9	74.52795702342732	12.116882291236212	8.587844496010682	4.767316189325789
10	39.017928096888014	34.87046632124352	12.188245017324135	13.923360564544327
11	32.42649162401857	26.726056136558697	23.379795924854573	17.467656314568167
12	31.680759083251214	23.515369210718713	28.74105979211037	16.062811913919706
13	25.741441240980322	32.44413363425411	22.49864903525223	19.315776089513335
14	17.35719507930958	35.86938554944531	28.765853968657616	18.007565402587495
15	15.890206300263834	29.13236275787533	34.23598970088051	20.741441240980325
16	17.631202517562542	27.81556947137544	32.48211958422073	22.071108426841285
17	18.011697765345367	25.242061095394003	26.813312565561525	29.9329285736991
18	23.73867573667313	26.29358848024413	25.706316157538385	24.26141962554436
19	25.167042817635654	29.149845831081727	25.552147239263807	20.130964112018816
20	24.758097841635145	29.173368511395786	23.744397469722493	22.324136177246576
21	23.781906608601673	29.347086684255697	24.58787628341651	22.28313042372612
22	19.637464636511016	29.973934327219553	24.210877650274963	26.17772338599447
23	20.96141008932261	28.065418481197753	22.526780889411615	28.446390540068023
24	17.558568295241425	29.933723258844847	27.512794430846498	24.99491401506723
25	19.421469213897456	27.42410756858133	25.4853936870212	27.669029530500016
26	20.867319368066372	32.11147843224514	21.903906672176486	25.117295527512002
27	18.143774436568233	29.32324612988334	26.28309863632029	26.249880797228137
28	21.450141453955943	27.64868559076894	27.704154613941956	23.197018341333163
29	20.055786897231318	29.58151880225055	27.73975650847134	22.62293779204679
30	22.853396484312917	26.897549191010523	27.859118217362283	22.38993610731428
31	26.566642296322197	31.117009440859533	21.247178867732604	21.06916939508567
32	27.392955910868118	28.536825709653833	22.91093168886487	21.15928669061318
33	24.67529164944849	26.03022982294415	27.255157506595886	22.039321021011478
34	22.726246860993673	26.979242823993133	26.14577704313551	24.14873327187768
35	23.23595791347468	26.496392129438316	27.325725547538067	22.941924409548935
36	24.69881432976255	26.97479258717696	29.22740710130646	19.09898598175403
37	23.65714104071967	29.935789440223783	25.332655202008965	21.074414317047584
38	25.669442766775802	27.12037890587749	25.520359833433993	21.689818493912714
39	25.48555262405035	25.45869226612416	26.495756381321723	22.559998728503768
40	26.135764010299116	24.438475476016404	25.579325471248293	23.846435042436188
41	20.930735242696844	22.470040370005403	29.509838202104326	27.089386185193426
42	26.89929749833116	24.34168282526463	24.52541403096093	24.233605645443276
43	22.311421214914652	26.33666041514352	26.59779395403541	24.754124415906418
44	21.826027527893448	24.249022537270733	25.999078165230937	27.925871769604882
45	23.072252773451158	27.097650910709177	25.384945484599005	24.44515083124066
46	20.844114561810613	27.04901617978957	29.508407768841984	22.598461489557835
47	20.988747258336247	25.181982898375665	27.14088178263772	26.688388060650368
48	22.410756858132807	22.168060014622206	31.762929527321276	23.65825359992371
49	24.655265583775705	20.976509107091772	29.25347277408691	25.114752535045614
50	20.674051940621126	24.52430147175689	28.144410184684826	26.65723640293716
51	21.66295813598652	23.684319272704155	29.650338535872088	25.002384055437236
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4103.0
1	3312.0
2	2521.0
3	1701.5
4	882.0
5	787.0
6	692.0
7	760.0
8	828.0
9	823.5
10	819.0
11	922.0
12	1025.0
13	1133.0
14	1241.0
15	1274.0
16	1307.0
17	1405.0
18	1503.0
19	1491.5
20	1480.0
21	1804.5
22	2129.0
23	2008.5
24	1888.0
25	2223.5
26	3427.5
27	4296.0
28	5424.0
29	6552.0
30	7260.5
31	7969.0
32	9115.0
33	10261.0
34	11955.0
35	13649.0
36	15124.0
37	16599.0
38	17380.0
39	18161.0
40	22178.5
41	26196.0
42	33698.5
43	41201.0
44	51530.0
45	61859.0
46	62913.5
47	63968.0
48	62277.0
49	60586.0
50	55869.5
51	51153.0
52	47759.5
53	44366.0
54	40052.5
55	35739.0
56	32161.5
57	28584.0
58	28285.5
59	27987.0
60	27152.0
61	26317.0
62	22667.0
63	19017.0
64	16182.0
65	13347.0
66	11384.0
67	9421.0
68	8022.5
69	6624.0
70	5496.5
71	4369.0
72	3521.5
73	2674.0
74	2253.5
75	1652.5
76	1472.0
77	1058.0
78	644.0
79	630.0
80	616.0
81	490.0
82	364.0
83	277.0
84	190.0
85	137.0
86	84.0
87	69.0
88	54.0
89	41.0
90	28.0
91	19.5
92	11.0
93	7.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.10617296694963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82172848271001	24.031267642213574
2	9.190577812512416	5.533862505794167
3	3.3893906130957996	3.0612474015125275
4	1.5535621529484607	1.8708724356629205
5	0.8490898702074309	1.2781423248474861
6	0.5016228590209709	0.9061166734716684
7	0.35459222584405586	0.7472790418797768
8	0.23734521162734912	0.5716444795304189
9	0.1800410226787258	0.4878311552920998
>10	2.450678355675773	18.313025882740156
>50	1.0857596405410979	23.644155936063434
>100	0.37659469701229975	14.941816275159663
>500	0.005304150662145068	1.1360181361507107
>1k	0.003712905463501547	3.476720109681371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	4924	0.7826059315299277	TruSeq Adapter, Index 23 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	4014	0.6379732350042913	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	3623	0.5758288566070123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3526	0.5604119647795544	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGT	3108	0.49397628659525095	No Hit
AAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1524	0.24222003242315393	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	1053	0.16736069169395085	No Hit
AATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	972	0.1544867923328777	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	876	0.1392288375345688	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	768	0.12206363838647127	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	742	0.11793127562859596	No Hit
AACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG	720	0.11443466098731683	TruSeq Adapter, Index 22 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	685	0.10887186496710004	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	651	0.10346800597603231	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.357481165962046E-4	0.0	0.0	1.0300708859150005	0.0
2	6.357481165962046E-4	0.0	0.0	1.2637083187641056	0.0
3	6.357481165962046E-4	0.0	0.0	1.6618455767824787	0.0
4	6.357481165962046E-4	0.0	0.0	3.776184875552306	0.0
5	6.357481165962046E-4	0.0	0.0	4.224705171810928	0.0
6	6.357481165962046E-4	0.0	0.0	5.510187863568454	0.0
7	6.357481165962046E-4	0.0	0.0	6.549953908261546	0.0
8	6.357481165962046E-4	0.0	0.0	7.115928669061318	0.0
9	6.357481165962046E-4	0.0	0.0	8.48231030865571	0.0
10	6.357481165962046E-4	0.0	0.0	9.787342254998569	0.0
11	6.357481165962046E-4	0.0	0.0	11.72526145141295	0.0
12	6.357481165962046E-4	0.0	0.0	12.535681363043961	0.0
13	6.357481165962046E-4	0.0	0.0	13.074636828888394	0.0
14	6.357481165962046E-4	0.0	0.0	13.54429575002384	0.0
15	6.357481165962046E-4	0.0	0.0	13.8192568104517	0.0
16	6.357481165962046E-4	0.0	0.0	14.335961092215264	0.0
17	6.357481165962046E-4	0.0	0.0	14.96153723894593	0.0
18	6.357481165962046E-4	0.0	0.0	15.856352713055088	0.0
19	7.946851457452558E-4	0.0	0.0	16.173273149178296	0.0
20	7.946851457452558E-4	0.0	0.0	16.541053434629198	0.0
21	7.946851457452558E-4	0.0	0.0	16.91296608283798	0.0
22	7.946851457452558E-4	0.0	0.0	17.29981881178677	0.0
23	7.946851457452558E-4	0.0	0.0	17.689532407260245	0.0
24	7.946851457452558E-4	0.0	0.0	17.98992339235195	0.0
25	7.946851457452558E-4	0.0	0.0	18.23325598397915	0.0
26	7.946851457452558E-4	0.0	0.0	18.45227121014654	0.0
27	7.946851457452558E-4	0.0	0.0	18.708318764105663	0.0
28	7.946851457452558E-4	0.0	0.0	18.948631552179027	0.0
29	7.946851457452558E-4	0.0	0.0	19.192917765981118	0.0
30	7.946851457452558E-4	0.0	0.0	19.532089386185195	0.0
31	7.946851457452558E-4	0.0	0.0	19.800216154359642	0.0
32	7.946851457452558E-4	0.0	0.0	20.039734257287265	0.0
33	9.536221748943068E-4	0.0	0.0	20.31644362503576	0.0
34	9.536221748943068E-4	0.0	0.0	20.623827839410026	0.0
35	9.536221748943068E-4	0.0	0.0	20.930894179725993	0.0
36	9.536221748943068E-4	0.0	0.0	21.173432086207445	0.0
37	9.536221748943068E-4	0.0	0.0	21.419784481388472	0.0
38	9.536221748943068E-4	0.0	0.0	21.678851838901426	0.0
39	9.536221748943068E-4	0.0	0.0	21.978924949934836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACG	45	3.8380676E-10	45.000004	1
ATTCACG	45	3.8380676E-10	45.000004	1
AGCCGAA	20	7.0308504E-4	45.0	42
CGTATCA	20	7.0308504E-4	45.0	29
CGAGTTA	20	7.0308504E-4	45.0	26
GTCGACT	20	7.0308504E-4	45.0	13
GATCGCT	20	7.0308504E-4	45.0	28
GGTCGAC	20	7.0308504E-4	45.0	12
CACGGAA	20	7.0308504E-4	45.0	4
TTAGCGA	25	3.8887854E-5	45.0	27
GGCACTA	25	3.8887854E-5	45.0	8
CAGCCGA	25	3.8887854E-5	45.0	15
TCCGACA	25	3.8887854E-5	45.0	18
CTATACG	40	6.8066583E-9	45.0	1
CGTTGCC	20	7.0308504E-4	45.0	19
TAGAACG	20	7.0308504E-4	45.0	1
ATTACGG	50	2.1827873E-11	45.0	2
CGCGCGA	25	3.8887854E-5	45.0	33
TACGGTA	20	7.0308504E-4	45.0	25
CGCGATC	25	3.8887854E-5	45.0	35
>>END_MODULE