##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644428
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17457031662187	31.0	31.0	34.0	28.0	34.0
2	31.384013419652778	31.0	31.0	34.0	30.0	34.0
3	31.304763293959915	31.0	31.0	34.0	28.0	34.0
4	35.29614169465014	37.0	35.0	37.0	33.0	37.0
5	34.9410872898136	35.0	35.0	37.0	32.0	37.0
6	35.006633789965676	36.0	35.0	37.0	32.0	37.0
7	34.859891252397475	36.0	35.0	37.0	32.0	37.0
8	35.26766683011911	37.0	35.0	37.0	33.0	37.0
9	35.76070872153289	39.0	35.0	39.0	32.0	39.0
10	35.658546804297764	37.0	35.0	39.0	30.0	39.0
11	36.082407964892894	37.0	35.0	39.0	31.0	39.0
12	35.78406586926701	37.0	35.0	39.0	31.0	39.0
13	35.650126934273494	37.0	35.0	39.0	30.0	39.0
14	36.6143649872445	38.0	35.0	40.0	31.0	41.0
15	36.78085061480879	38.0	35.0	40.0	31.0	41.0
16	36.717127437044944	38.0	35.0	40.0	31.0	41.0
17	36.68410900829883	38.0	35.0	40.0	31.0	41.0
18	36.451308757533816	38.0	35.0	40.0	31.0	41.0
19	36.47578627868435	38.0	35.0	40.0	30.0	41.0
20	36.52671516445592	38.0	35.0	40.0	31.0	41.0
21	36.63434394532826	38.0	35.0	40.0	31.0	41.0
22	36.6024427864711	38.0	35.0	40.0	31.0	41.0
23	36.561262080480674	38.0	35.0	40.0	31.0	41.0
24	36.177282799630056	38.0	35.0	40.0	30.0	41.0
25	36.24831323282042	38.0	35.0	40.0	30.0	41.0
26	36.15532379102088	38.0	35.0	40.0	30.0	41.0
27	36.12447938326702	38.0	35.0	40.0	30.0	41.0
28	36.03396190109679	38.0	35.0	40.0	30.0	41.0
29	35.828267859248825	38.0	34.0	40.0	29.0	41.0
30	35.86965960510717	38.0	35.0	40.0	30.0	41.0
31	35.706916831670874	38.0	34.0	40.0	29.0	41.0
32	35.65322735821535	38.0	34.0	40.0	29.0	41.0
33	35.460124327310425	38.0	34.0	40.0	28.0	41.0
34	35.58514682788457	38.0	34.0	40.0	29.0	41.0
35	35.45428348861316	38.0	34.0	40.0	28.0	41.0
36	35.35560062567114	38.0	34.0	40.0	27.0	41.0
37	35.22104564047496	38.0	34.0	40.0	27.0	41.0
38	34.94766676804856	37.0	34.0	40.0	26.0	41.0
39	34.850818089840914	37.0	33.0	40.0	26.0	41.0
40	34.47297448279715	37.0	33.0	40.0	24.0	41.0
41	34.80081871054641	37.0	33.0	40.0	26.0	41.0
42	34.781804949505606	37.0	33.0	40.0	26.0	41.0
43	34.808808742016176	37.0	33.0	40.0	26.0	41.0
44	34.77159744765901	37.0	33.0	40.0	26.0	41.0
45	34.38078264755721	37.0	33.0	40.0	24.0	41.0
46	34.26994792280907	36.0	33.0	40.0	24.0	41.0
47	34.0808903399604	36.0	33.0	40.0	24.0	41.0
48	34.06516942156455	36.0	33.0	39.0	24.0	41.0
49	33.97288448050054	36.0	33.0	39.0	24.0	41.0
50	33.791574853979036	36.0	33.0	39.0	23.0	40.0
51	31.80418293432315	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	7.0
11	9.0
12	9.0
13	13.0
14	26.0
15	52.0
16	125.0
17	246.0
18	454.0
19	962.0
20	1472.0
21	2172.0
22	2992.0
23	3861.0
24	4889.0
25	5850.0
26	7014.0
27	8758.0
28	11435.0
29	14482.0
30	18986.0
31	24209.0
32	32084.0
33	42350.0
34	64391.0
35	70270.0
36	68072.0
37	85213.0
38	102630.0
39	71383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.615112937364607	38.4371877075484	19.25537065428566	23.69232870080133
2	21.810039290657762	38.07717852110709	18.272638681124967	21.84014350711018
3	19.7753666817705	28.562228829287367	28.127734983582343	23.534669505359794
4	26.15559845320191	29.123656948487653	17.831472251360896	26.889272346949543
5	16.39096997647526	38.42958406524856	18.69906335540976	26.48038260286642
6	21.518307708541528	33.86615727435803	24.698802659102338	19.9167323579981
7	73.13229716896224	12.996486806904727	7.816078755113061	6.055137269019968
8	75.30957686506483	5.675886212268865	6.131639221138746	12.882897701527558
9	70.98263886733662	11.03691956277505	10.893846946439323	7.086594623449012
10	40.191146256835516	28.99082597280069	13.802162537940625	17.01586523242317
11	31.660790654658083	23.923075968145394	24.95158497147858	19.464548405717938
12	29.873779537822685	22.502901798183817	31.43857808785466	16.18474057613884
13	23.93114513956563	32.26271980733301	22.92405047577076	20.882084577330595
14	16.40493585008721	31.71913697108133	33.79850037552682	18.077426803304636
15	15.225285058998056	28.806631617496446	30.22696096383149	25.74112235967401
16	16.51092131316454	25.152228022370227	35.007634677574536	23.3292159868907
17	16.004425630171255	26.80982204373491	28.19663329340128	28.989119032692557
18	20.795806513683452	26.590557828027332	26.925118089220206	25.688517569069003
19	21.048744002433164	29.79060500164487	29.07927650567635	20.08137449024561
20	24.054200003724233	30.973980025697205	24.04209624659388	20.92972372398468
21	19.55874046441185	28.573091175429994	26.038905820355417	25.829262539802738
22	19.647035820914052	29.75863866871086	25.55537624063511	25.038949269739984
23	21.607534123284523	27.838641399815028	21.76333120224447	28.790493274655976
24	17.796402390957564	33.33669548809176	26.379052430993067	22.487849689957606
25	19.283612754256488	25.25154090138852	28.365465187732376	27.099381156622616
26	23.108244831075	31.258728671007468	21.162022755063408	24.471003742854126
27	18.02683930555469	27.43270000682776	28.67333511268908	25.867125574928462
28	19.78607385153966	25.505254272005562	31.203485882053545	23.505185994401238
29	18.986294822695477	28.42753573711881	27.126381845605717	25.459787594580003
30	21.60132706834588	26.724475038328567	25.19614293606113	26.478054957264423
31	25.8390386513311	27.518512541354507	22.84413464343573	23.798314163878665
32	25.755553762406354	23.944490307683715	22.77678809735145	27.52316783255849
33	23.892816575319507	27.186900631257487	25.082709007057417	23.83757378636558
34	18.294984078904083	24.36020781219935	24.59995530920444	32.74485279969213
35	20.202877590669555	23.62544768383745	31.46278560211536	24.70888912337763
36	22.648457236495002	23.297249653956687	29.201245135220695	24.853047974327623
37	22.605783733791828	26.23458322729614	23.191884896373217	27.967748142538813
38	22.419727262005996	28.01600799468676	28.009180234254256	21.55508450905299
39	23.618619923404943	24.16251311240356	26.1757713817525	26.043095582439
40	21.55663627278765	28.695835686841665	25.60829138398704	24.139236656383645
41	18.885895709062922	20.950827710776068	31.41157739887156	28.751699181289453
42	24.28634385842949	21.896938059798767	26.837133085464938	26.979584996306805
43	22.495453332257444	22.59507656402267	28.189029651101443	26.720440452618444
44	21.49456572340122	21.79033189122757	26.888186112335283	29.82691627303593
45	25.921282129268125	27.652274575282267	24.80354671119194	21.622896584257667
46	19.54136071058365	23.837884139112514	34.326720750805364	22.294034399498468
47	21.89088618123359	26.948239368866655	25.140589794360267	26.020284655539488
48	20.890464101497763	20.75515030383534	34.13476757682783	24.219618017839075
49	23.25799003146977	20.890308925124295	30.14145878205168	25.710242261354253
50	21.150229350679982	22.8877392043797	26.456795794099573	29.505235650840746
51	18.8644813695246	22.965948096606603	32.120578249238086	26.048992284630714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3222.0
1	2571.5
2	1921.0
3	1327.5
4	734.0
5	687.0
6	640.0
7	682.0
8	724.0
9	808.0
10	892.0
11	917.5
12	943.0
13	967.0
14	991.0
15	1068.5
16	1146.0
17	1193.0
18	1240.0
19	1312.5
20	1385.0
21	1483.0
22	1581.0
23	1940.5
24	2300.0
25	2290.5
26	3142.0
27	4003.0
28	4733.5
29	5464.0
30	6671.5
31	7879.0
32	8334.0
33	8789.0
34	10894.5
35	13000.0
36	13933.0
37	14866.0
38	17302.5
39	19739.0
40	22031.5
41	24324.0
42	31737.0
43	39150.0
44	62024.5
45	84899.0
46	81580.0
47	78261.0
48	70999.0
49	63737.0
50	57195.0
51	50653.0
52	47208.5
53	43764.0
54	38483.5
55	33203.0
56	30492.5
57	27782.0
58	24906.5
59	22031.0
60	22004.5
61	21978.0
62	19590.5
63	17203.0
64	15014.0
65	12825.0
66	11299.5
67	9774.0
68	8677.0
69	7580.0
70	6262.0
71	4944.0
72	3963.5
73	2983.0
74	2673.5
75	1913.0
76	1462.0
77	1155.0
78	848.0
79	640.5
80	433.0
81	352.0
82	271.0
83	192.0
84	113.0
85	84.0
86	55.0
87	38.0
88	21.0
89	16.5
90	12.0
91	10.0
92	8.0
93	8.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.040541963783564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41062368684068	20.418551365236162
2	9.433959093298805	4.9133081530733005
3	3.308246960119892	2.5844563137468435
4	1.5842653899777224	1.6502051747793924
5	0.9132659655176256	1.1890970349578522
6	0.5962026985139551	0.9315264833744188
7	0.3899701259463873	0.7108523400530202
8	0.28184494337036986	0.5871516060093055
9	0.2592427988224658	0.6075740683390609
>10	3.18082787396803	20.4538527107965
>50	1.3077265832887228	24.34682817152357
>100	0.32243671027008836	11.522612984011465
>500	0.005393922662510772	0.8889599701606278
>1k	0.003595948441673848	1.9659811699637337
>5k	5.993247402789747E-4	0.9215780589533895
>10k+	0.001797974220836924	6.307464395021379
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	14950	2.319886783317919	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGT	12943	2.0084478017714935	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	12522	1.943118548542273	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	5905	0.9163164853172115	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	3010	0.46708088413290544	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2712	0.42083832484001316	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT	2338	0.36280236116369863	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	1849	0.2869211145387848	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTAT	1525	0.23664396953577438	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	1163	0.18047012234105286	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	906	0.1405897943602699	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	748	0.11607192735262899	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	702	0.10893381417318926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.103527469321631E-4	0.0	0.0	1.234738403669611	0.0
2	3.103527469321631E-4	0.0	0.0	1.4662615528810046	0.0
3	3.103527469321631E-4	0.0	0.0	2.1606758241417197	0.0
4	3.103527469321631E-4	0.0	0.0	5.760457335807879	0.0
5	3.103527469321631E-4	0.0	0.0	6.091603716784498	0.0
6	3.103527469321631E-4	0.0	0.0	7.653298739347142	0.0
7	3.103527469321631E-4	0.0	0.0	8.848467167782902	0.0
8	3.103527469321631E-4	0.0	0.0	9.458310315504603	0.0
9	3.103527469321631E-4	0.0	0.0	12.661461016591458	0.0
10	3.103527469321631E-4	0.0	0.0	13.954390560310848	0.0
11	3.103527469321631E-4	0.0	0.0	18.048098468719548	0.0
12	3.103527469321631E-4	0.0	0.0	18.783634478948773	0.0
13	3.103527469321631E-4	0.0	0.0	19.2181283246538	0.0
14	3.103527469321631E-4	0.0	0.0	19.807643367451444	0.0
15	3.103527469321631E-4	0.0	0.0	20.131186106128226	0.0
16	3.103527469321631E-4	0.0	0.0	20.63473343802566	0.0
17	3.103527469321631E-4	0.0	0.0	21.189333796793434	0.0
18	3.103527469321631E-4	0.0	0.0	22.040321028881426	0.0
19	3.103527469321631E-4	0.0	0.0	22.35858777086036	0.0
20	3.103527469321631E-4	0.0	0.0	22.694544619414426	0.0
21	3.103527469321631E-4	0.0	0.0	23.043381106966176	0.0
22	3.103527469321631E-4	0.0	0.0	23.376699957171322	0.0
23	3.103527469321631E-4	0.0	0.0	23.76215806886107	0.0
24	3.103527469321631E-4	0.0	0.0	24.01478520486385	0.0
25	3.103527469321631E-4	0.0	0.0	24.22427330904306	0.0
26	3.103527469321631E-4	0.0	0.0	24.4162264830206	0.0
27	3.103527469321631E-4	0.0	0.0	24.65178421794211	0.0
28	3.103527469321631E-4	0.0	0.0	24.868720788047696	0.0
29	3.103527469321631E-4	0.0	0.0	25.097295586163234	0.0
30	3.103527469321631E-4	0.0	0.0	25.446442426461918	0.0
31	3.103527469321631E-4	0.0	0.0	25.683241572371156	0.0
32	3.103527469321631E-4	0.0	0.0	25.906385197415382	0.0
33	3.103527469321631E-4	0.0	0.0	26.157770922430434	0.0
34	3.103527469321631E-4	0.0	0.0	26.382311134835856	0.0
35	3.103527469321631E-4	0.0	0.0	26.648283438956717	0.0
36	3.103527469321631E-4	0.0	0.0	26.861340599725647	0.0
37	3.103527469321631E-4	0.0	0.0	27.064311296219284	0.0
38	3.103527469321631E-4	0.0	0.0	27.28140304269833	0.0
39	3.103527469321631E-4	0.0	0.0	27.5015983166467	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	25	3.888891E-5	45.000004	25
CGAACCG	25	3.888891E-5	45.000004	1
GGTCGAT	25	3.888891E-5	45.000004	8
ACGTTAC	25	3.888891E-5	45.000004	43
CCAATCG	25	3.888891E-5	45.000004	1
GTTAGTA	25	3.888891E-5	45.000004	15
CGATTTA	25	3.888891E-5	45.000004	22
CTAAGTC	25	3.888891E-5	45.000004	19
TAAGTCG	25	3.888891E-5	45.000004	20
TAAGTAC	25	3.888891E-5	45.000004	28
TCGTGTG	25	3.888891E-5	45.000004	41
ACGATAT	25	3.888891E-5	45.000004	30
TACGATA	25	3.888891E-5	45.000004	29
CGTAGCG	50	2.1827873E-11	45.000004	1
CTACGAT	25	3.888891E-5	45.000004	28
CGCAGGT	25	3.888891E-5	45.000004	39
CGTACGA	25	3.888891E-5	45.000004	23
TACTATC	25	3.888891E-5	45.000004	13
CGTCGGA	25	3.888891E-5	45.000004	23
TAAGGGT	50	2.1827873E-11	45.000004	4
>>END_MODULE