Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548764_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 578538 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC | 7630 | 1.3188416318374938 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT | 6776 | 1.1712281647877927 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGT | 6149 | 1.0628515326564547 | No Hit |
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT | 3799 | 0.6566552240302279 | TruSeq Adapter, Index 20 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2779 | 0.48034874113714227 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT | 1377 | 0.23801375190566568 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT | 1265 | 0.21865460868603273 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT | 1247 | 0.21554331781144884 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1018 | 0.1759607839070208 | No Hit |
AACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG | 940 | 0.1624785234504907 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT | 835 | 0.14432932668208484 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 712 | 0.1230688390390951 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTAT | 696 | 0.1203032471505761 | No Hit |
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 682 | 0.11788335424812199 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AACGTTG | 30 | 2.1636242E-6 | 45.000004 | 1 |
AAATCCG | 30 | 2.1636242E-6 | 45.000004 | 1 |
GTATTCG | 25 | 3.888394E-5 | 45.000004 | 11 |
ACGTTCC | 25 | 3.888394E-5 | 45.000004 | 43 |
TTGTCGT | 25 | 3.888394E-5 | 45.000004 | 43 |
AGGGTAC | 100 | 0.0 | 45.000004 | 6 |
TAACGCG | 60 | 0.0 | 45.000004 | 1 |
ATCGTTC | 25 | 3.888394E-5 | 45.000004 | 35 |
AGTATAC | 25 | 3.888394E-5 | 45.000004 | 26 |
ATATCGT | 25 | 3.888394E-5 | 45.000004 | 33 |
CACTAGA | 25 | 3.888394E-5 | 45.000004 | 26 |
TATTCGT | 25 | 3.888394E-5 | 45.000004 | 12 |
TCGTACT | 25 | 3.888394E-5 | 45.000004 | 42 |
CGGTCGT | 25 | 3.888394E-5 | 45.000004 | 30 |
GCTACGA | 50 | 2.1827873E-11 | 45.000004 | 9 |
CAATCGT | 30 | 2.1636242E-6 | 45.000004 | 15 |
CCACGAT | 25 | 3.888394E-5 | 45.000004 | 45 |
TATCGTT | 25 | 3.888394E-5 | 45.000004 | 34 |
CAATACG | 25 | 3.888394E-5 | 45.000004 | 1 |
CGGAATA | 20 | 7.0303795E-4 | 45.0 | 31 |