FastQCFastQC Report
Sat 18 Jun 2016
SRR3548764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578538
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC76301.3188416318374938No Hit
AATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT67761.1712281647877927No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGT61491.0628515326564547No Hit
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT37990.6566552240302279TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27790.48034874113714227No Hit
AATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT13770.23801375190566568No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT12650.21865460868603273No Hit
AAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT12470.21554331781144884No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10180.1759607839070208No Hit
AACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG9400.1624785234504907No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT8350.14432932668208484No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7120.1230688390390951No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTAT6960.1203032471505761No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA6820.11788335424812199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG302.1636242E-645.0000041
AAATCCG302.1636242E-645.0000041
GTATTCG253.888394E-545.00000411
ACGTTCC253.888394E-545.00000443
TTGTCGT253.888394E-545.00000443
AGGGTAC1000.045.0000046
TAACGCG600.045.0000041
ATCGTTC253.888394E-545.00000435
AGTATAC253.888394E-545.00000426
ATATCGT253.888394E-545.00000433
CACTAGA253.888394E-545.00000426
TATTCGT253.888394E-545.00000412
TCGTACT253.888394E-545.00000442
CGGTCGT253.888394E-545.00000430
GCTACGA502.1827873E-1145.0000049
CAATCGT302.1636242E-645.00000415
CCACGAT253.888394E-545.00000445
TATCGTT253.888394E-545.00000434
CAATACG253.888394E-545.0000041
CGGAATA207.0303795E-445.031