##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548764_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578538
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417384856310218	31.0	31.0	34.0	30.0	34.0
2	31.5866459938673	31.0	31.0	34.0	30.0	34.0
3	31.78343514168473	31.0	31.0	34.0	30.0	34.0
4	35.58617411475132	37.0	35.0	37.0	33.0	37.0
5	35.17951975496856	37.0	35.0	37.0	32.0	37.0
6	35.17650353131514	37.0	35.0	37.0	32.0	37.0
7	35.13084533773063	37.0	35.0	37.0	32.0	37.0
8	35.4630067515012	37.0	35.0	37.0	33.0	37.0
9	36.32092792521839	39.0	37.0	39.0	32.0	39.0
10	36.05362655521331	38.0	35.0	39.0	31.0	39.0
11	36.30445709702733	38.0	35.0	39.0	32.0	39.0
12	35.94225789835758	37.0	35.0	39.0	31.0	39.0
13	35.77158285194749	37.0	35.0	39.0	31.0	39.0
14	36.80693575875742	38.0	35.0	40.0	31.0	41.0
15	36.85566030234833	38.0	35.0	40.0	32.0	41.0
16	36.85328189332421	38.0	35.0	40.0	32.0	41.0
17	36.81348156905856	38.0	35.0	40.0	32.0	41.0
18	36.60316003443162	38.0	35.0	40.0	31.0	41.0
19	36.631173751767385	38.0	35.0	40.0	31.0	41.0
20	36.62118305107011	38.0	35.0	40.0	31.0	41.0
21	36.70720678676249	38.0	35.0	40.0	31.0	41.0
22	36.659073388437754	38.0	35.0	40.0	31.0	41.0
23	36.57822995205155	38.0	35.0	40.0	31.0	41.0
24	36.23094593613557	38.0	35.0	40.0	30.0	41.0
25	36.31744155094393	38.0	35.0	40.0	30.0	41.0
26	36.16193923303223	38.0	35.0	40.0	30.0	41.0
27	36.072980512948156	38.0	35.0	40.0	30.0	41.0
28	36.057157870355965	38.0	35.0	40.0	30.0	41.0
29	35.717446390729734	38.0	34.0	40.0	29.0	41.0
30	35.76125682323374	38.0	34.0	40.0	29.0	41.0
31	35.59831679163685	38.0	34.0	40.0	29.0	41.0
32	35.44888494792045	38.0	34.0	40.0	28.0	41.0
33	35.5453436075072	38.0	34.0	40.0	29.0	41.0
34	35.36460353511783	38.0	34.0	40.0	27.0	41.0
35	35.39062429779894	38.0	34.0	40.0	28.0	41.0
36	35.153329599784286	38.0	34.0	40.0	27.0	41.0
37	35.047073830932455	37.0	34.0	40.0	26.0	41.0
38	34.98132534077278	37.0	34.0	40.0	26.0	41.0
39	34.86093739737061	37.0	33.0	40.0	26.0	41.0
40	34.74780740418088	37.0	33.0	40.0	25.0	41.0
41	34.845448354299975	37.0	34.0	40.0	26.0	41.0
42	34.797793749070934	37.0	34.0	40.0	26.0	41.0
43	34.63170785670086	37.0	33.0	40.0	25.0	41.0
44	34.50367132323201	37.0	33.0	40.0	25.0	41.0
45	34.11421030252118	36.0	33.0	40.0	23.0	41.0
46	34.05880339752964	36.0	33.0	40.0	23.0	41.0
47	33.98367782237295	36.0	33.0	40.0	23.0	41.0
48	33.9993673708555	36.0	33.0	40.0	23.0	41.0
49	33.881853914522466	36.0	33.0	39.0	23.0	41.0
50	33.59858125136119	35.0	33.0	39.0	23.0	40.0
51	31.625680594878816	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	7.0
11	8.0
12	12.0
13	13.0
14	31.0
15	49.0
16	113.0
17	220.0
18	443.0
19	836.0
20	1453.0
21	2011.0
22	2815.0
23	3784.0
24	4524.0
25	5576.0
26	6594.0
27	8138.0
28	10268.0
29	13074.0
30	16446.0
31	20844.0
32	26982.0
33	36088.0
34	56238.0
35	62400.0
36	57942.0
37	76500.0
38	95071.0
39	70054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.338574129962076	35.64899799148889	19.87769169873025	25.134736179818784
2	21.32409625642568	36.47141587933723	19.47426098199254	22.73022688224455
3	19.96861053206531	31.611925232223292	23.89523246528318	24.524231770428216
4	23.327940429150722	31.37667707220615	18.221275007000404	27.074107491642728
5	17.58121333430129	37.73010588759943	17.94713571105096	26.741545067048317
6	20.905281934808084	35.49014930739208	21.87514044021309	21.729428317586745
7	77.2855024216214	10.433195399437894	7.013195330298096	5.268106848642613
8	79.3802654276815	6.007902678821443	5.2077823755742925	9.404049517922763
9	73.6864302777	10.442702121554676	10.058630548036602	5.812237052708724
10	42.328939499220446	28.30773432341523	12.914795570904591	16.448530606459734
11	32.58403078103772	24.32459060597575	23.371498501394896	19.719880111591635
12	30.15117416660617	23.4930116949967	29.830192658044936	16.5256214803522
13	24.173001600586304	32.215342812399534	22.559970131607603	21.05168545540656
14	18.483660537423642	32.603735623243416	30.442598411858857	18.47000542747408
15	15.994973536742615	28.51394376860293	32.16054952310825	23.330533171546207
16	17.651908776951558	25.930880944726187	32.62689745530976	23.790312823012492
17	17.71880153075511	26.04928284745341	27.743380728664324	28.48853489312716
18	21.352097874296934	27.41565808987482	26.398784522365	24.833459513463247
19	22.44416097127587	30.507071272759955	27.11991260729632	19.92885514866785
20	25.688891654480777	29.070000587688277	24.426571806864892	20.814535950966057
21	21.777999025128857	28.895595449218547	25.26955878438408	24.056846741268508
22	20.691812810913028	29.725964413746375	24.13428331414704	25.44793946119356
23	21.096972022581056	28.84408630029488	22.54337658027649	27.51556509684757
24	18.744836121395654	31.81536908552247	27.449536590509183	21.99025820257269
25	19.222937819123377	26.842834869965326	26.231120514123536	27.703106796787765
26	22.923472615454816	32.38559956303648	21.259104847045485	23.431822974463216
27	19.575205085923482	28.31551255060169	27.768616754647056	24.34066560882777
28	19.59110723928247	27.23278332624651	27.537689831955724	25.638419602515306
29	19.310399662597792	31.47520128323464	26.550200678261408	22.664198375906164
30	22.0851525742475	25.97184627457488	27.9340337194791	24.008967431698522
31	25.66209998306075	28.666397021457534	19.740622050755523	25.930880944726187
32	23.39189474157272	28.150786983741778	23.079210008677045	25.378108266008454
33	23.480393682005328	25.932955138642576	25.055052563530833	25.53159861582126
34	17.90254054184859	28.867766680840322	23.126916468753997	30.10277630855709
35	19.575032236430452	24.81168047734116	27.338048667503255	28.27523861872513
36	23.4994071262389	26.72097597737746	26.569386971988013	23.21022992439563
37	19.849171532379895	29.884813097843182	23.59602999284403	26.669985376932893
38	21.17613709038991	27.16468062599172	25.677310738447606	25.98187154517076
39	20.506518154382253	26.568522724522847	26.94239617795201	25.982562943142888
40	21.376815351800573	25.714646228942613	25.201110385143238	27.707428034113573
41	19.81754007515496	21.38511212746613	28.60503545143102	30.19231234594789
42	22.780007536237896	23.971286242217452	23.096494957980287	30.152211263564364
43	21.346220991533833	24.276192747926668	27.73352830756147	26.644057952978024
44	21.1915206952698	24.030573618327576	25.900459433952484	28.877446252450138
45	24.226757792919393	27.136679008120467	24.924896895277406	23.711666303682733
46	19.69844677445561	25.203357428552664	31.735512619741485	23.36268317725024
47	21.77471488476124	26.501802820212326	25.181059843951477	26.54242245107495
48	21.44128821270167	21.591148723160796	31.426803425185557	25.540759638951975
49	22.239334322032434	22.553574700365402	28.616443517971163	26.590647459631
50	20.786707182587833	24.775554933297382	25.733486823683144	28.70425106043164
51	19.484459102081452	23.680726244429927	29.824661474267895	27.010153179220726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3243.0
1	2604.5
2	1966.0
3	1339.5
4	713.0
5	673.0
6	633.0
7	672.5
8	712.0
9	761.5
10	811.0
11	879.0
12	947.0
13	1097.0
14	1247.0
15	1262.0
16	1277.0
17	1334.0
18	1391.0
19	1367.0
20	1343.0
21	1282.5
22	1222.0
23	1543.5
24	1865.0
25	2323.5
26	3183.5
27	3585.0
28	4233.5
29	4882.0
30	5787.5
31	6693.0
32	8213.5
33	9734.0
34	10836.5
35	11939.0
36	12359.5
37	12780.0
38	14158.5
39	15537.0
40	18750.0
41	21963.0
42	28644.0
43	35325.0
44	50018.0
45	64711.0
46	62987.5
47	61264.0
48	58002.0
49	54740.0
50	51310.0
51	47880.0
52	43637.5
53	39395.0
54	35884.5
55	32374.0
56	30444.5
57	28515.0
58	27226.0
59	25937.0
60	23292.5
61	20648.0
62	18537.0
63	16426.0
64	14331.5
65	12237.0
66	11443.5
67	10650.0
68	8567.0
69	6484.0
70	5654.5
71	4825.0
72	4236.0
73	3647.0
74	3111.5
75	2093.5
76	1611.0
77	1302.0
78	993.0
79	769.5
80	546.0
81	411.5
82	277.0
83	198.0
84	119.0
85	77.5
86	36.0
87	29.5
88	23.0
89	21.5
90	20.0
91	12.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	578538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.04714429651938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26030542616947	21.43764917847804
2	9.204749748794072	4.979243893379676
3	3.075695846893915	2.495663681494353
4	1.4648210446340055	1.584769046511768
5	0.7977438618503779	1.07883466715649
6	0.5418362358745303	0.8793073714068799
7	0.36842599162512285	0.6975409670651057
8	0.28801621662491284	0.6232012936633282
9	0.24378535427128212	0.5934327888918118
>10	3.0655888144562127	20.56088064486121
>50	1.3114232172603548	25.534614177781133
>100	0.3674026133958971	13.150734312979093
>500	0.0044649623155751375	0.856565204769192
>1k	0.0038271105562072607	1.9814000758860366
>5k	0.0019135552781036303	3.5461626956758794
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC	7630	1.3188416318374938	No Hit
AATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	6776	1.1712281647877927	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGT	6149	1.0628515326564547	No Hit
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT	3799	0.6566552240302279	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2779	0.48034874113714227	No Hit
AATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT	1377	0.23801375190566568	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT	1265	0.21865460868603273	No Hit
AAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT	1247	0.21554331781144884	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1018	0.1759607839070208	No Hit
AACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG	940	0.1624785234504907	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT	835	0.14432932668208484	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.1230688390390951	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTAT	696	0.1203032471505761	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	682	0.11788335424812199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.456989860648739E-4	0.0	0.0	0.971587000335328	0.0
2	3.456989860648739E-4	0.0	0.0	1.1980198362078205	0.0
3	3.456989860648739E-4	0.0	0.0	1.6934064832387847	0.0
4	3.456989860648739E-4	0.0	0.0	4.534879299199016	0.0
5	3.456989860648739E-4	0.0	0.0	4.83874870795004	0.0
6	3.456989860648739E-4	0.0	0.0	6.276165092007785	0.0
7	3.456989860648739E-4	0.0	0.0	7.435639491269372	0.0
8	3.456989860648739E-4	0.0	0.0	8.053749278353367	0.0
9	3.456989860648739E-4	0.0	0.0	10.287310427318516	0.0
10	3.456989860648739E-4	0.0	0.0	11.621189965049833	0.0
11	3.456989860648739E-4	0.0	0.0	14.524715749008708	0.0
12	3.456989860648739E-4	0.0	0.0	15.220434958464267	0.0
13	3.456989860648739E-4	0.0	0.0	15.5675167404734	0.0
14	3.456989860648739E-4	0.0	0.0	16.025913595995423	0.0
15	3.456989860648739E-4	0.0	0.0	16.29521310613996	0.0
16	3.456989860648739E-4	0.0	0.0	16.7760803957562	0.0
17	3.456989860648739E-4	0.0	0.0	17.33300146230671	0.0
18	3.456989860648739E-4	0.0	0.0	18.15853064102963	0.0
19	3.456989860648739E-4	0.0	0.0	18.47709225668841	0.0
20	3.456989860648739E-4	0.0	0.0	18.79893801271481	0.0
21	3.456989860648739E-4	0.0	0.0	19.151032430021882	0.0
22	3.456989860648739E-4	0.0	0.0	19.493965824198238	0.0
23	3.456989860648739E-4	0.0	0.0	19.884778527944576	0.0
24	3.456989860648739E-4	0.0	0.0	20.15165814518666	0.0
25	3.456989860648739E-4	0.0	0.0	20.37152270032392	0.0
26	3.456989860648739E-4	0.0	0.0	20.59052300799602	0.0
27	3.456989860648739E-4	0.0	0.0	20.815400198431217	0.0
28	3.456989860648739E-4	0.0	0.0	21.029215021312343	0.0
29	3.456989860648739E-4	0.0	0.0	21.245622586588954	0.0
30	3.456989860648739E-4	0.0	0.0	21.586136087862855	0.0
31	3.456989860648739E-4	0.0	0.0	21.823804140782457	0.0
32	3.456989860648739E-4	0.0	0.0	22.05559531093895	0.0
33	3.456989860648739E-4	0.0	0.0	22.278571156950797	0.0
34	3.456989860648739E-4	0.0	0.0	22.503966895865094	0.0
35	3.456989860648739E-4	0.0	0.0	22.778624740293637	0.0
36	3.456989860648739E-4	0.0	0.0	23.021305428511177	0.0
37	3.456989860648739E-4	0.0	0.0	23.241169983648437	0.0
38	3.456989860648739E-4	0.0	0.0	23.494913039420055	0.0
39	3.456989860648739E-4	0.0	0.0	23.72272867123681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.1636242E-6	45.000004	1
AAATCCG	30	2.1636242E-6	45.000004	1
GTATTCG	25	3.888394E-5	45.000004	11
ACGTTCC	25	3.888394E-5	45.000004	43
TTGTCGT	25	3.888394E-5	45.000004	43
AGGGTAC	100	0.0	45.000004	6
TAACGCG	60	0.0	45.000004	1
ATCGTTC	25	3.888394E-5	45.000004	35
AGTATAC	25	3.888394E-5	45.000004	26
ATATCGT	25	3.888394E-5	45.000004	33
CACTAGA	25	3.888394E-5	45.000004	26
TATTCGT	25	3.888394E-5	45.000004	12
TCGTACT	25	3.888394E-5	45.000004	42
CGGTCGT	25	3.888394E-5	45.000004	30
GCTACGA	50	2.1827873E-11	45.000004	9
CAATCGT	30	2.1636242E-6	45.000004	15
CCACGAT	25	3.888394E-5	45.000004	45
TATCGTT	25	3.888394E-5	45.000004	34
CAATACG	25	3.888394E-5	45.000004	1
CGGAATA	20	7.0303795E-4	45.0	31
>>END_MODULE