FastQCFastQC Report
Sat 18 Jun 2016
SRR3548763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590053
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCC70301.1914183980083144No Hit
AATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT64411.0915968565535639No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGT59541.009061897829517No Hit
CTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTGCT36620.6206222152925245TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30060.5094457616519195No Hit
AATGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT13510.22896248303118533No Hit
AATGATCTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCT11620.19693146208899875No Hit
AAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCT11460.19421984126849623No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10160.17218792210191286No Hit
AACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTTCTG8960.15185076594814365No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7610.12897146527515324No Hit
AAAAACTGTCTCTTATACACATCTGACGCCATCATCCTCGTATGCCGTCTT7280.12337874733286672No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA7220.12236188952517825No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCATCATCCTCGTAT6680.11321016925598208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG351.210592E-745.0000041
GACACGA1200.045.00000425
TCACACG551.8189894E-1245.0000041
GTTTAGC351.210592E-745.00000441
ACCGAGT351.210592E-745.00000431
ATAGACG351.210592E-745.0000041
CACGAAA302.1636988E-645.00000442
TATGCGG700.045.0000042
CCGTACC302.1636988E-645.00000430
TCCGTAC302.1636988E-645.00000429
TTACGAG302.1636988E-645.0000041
AACCGTA207.0304924E-445.021
ATGGGCG406.8048394E-945.05
GGTACCC207.0304924E-445.035
GATCGCT207.0304924E-445.023
TAGGTTA207.0304924E-445.019
GACGTCG207.0304924E-445.043
CGGTTAG207.0304924E-445.01
CCAATGC207.0304924E-445.026
ATAGCCG207.0304924E-445.01