FastQCFastQC Report
Sat 18 Jun 2016
SRR3548762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557216
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC85231.529568425888704No Hit
AATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT79091.4193777637397347No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGT69481.246913225750876No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT50680.9095216217768334TruSeq Adapter, Index 19 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28660.5143427324412795No Hit
AATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT16160.29001320852236834No Hit
AATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT14320.2569919026015046No Hit
AAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT13280.23832768621145123No Hit
AATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC12290.22056078791707345No Hit
AACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG11420.2049474530523172TruSeq Adapter, Index 13 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9970.17892522827772353No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT8740.1568512031241027No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTAT8330.14949319473956238No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7040.12634238787113075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG406.8048394E-945.0000041
AGCGTTG207.0301525E-445.00000420
CTATGCA207.0301525E-445.00000422
AAGTACG207.0301525E-445.0000041
ATCTCGC207.0301525E-445.00000416
TGCCCAT207.0301525E-445.00000433
CTCAATG302.1634805E-645.0000041
CTCAACT207.0301525E-445.00000430
ACGTTAG207.0301525E-445.0000041
TAGACGT207.0301525E-445.00000428
ACCGCAT207.0301525E-445.00000421
TAGGTCG302.1634805E-645.00000433
CGGTTAT207.0301525E-445.0000046
ATTACGG551.8189894E-1245.0000042
ATTACCG207.0301525E-445.0000041
GATTTCG207.0301525E-445.00000435
CACCTCG302.1634805E-645.0000041
CCACTTT207.0301525E-445.00000431
GTAGCAA207.0301525E-445.00000417
TTAAGCG207.0301525E-445.00000425