Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548762_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 557216 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC | 8523 | 1.529568425888704 | No Hit |
AATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT | 7909 | 1.4193777637397347 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGT | 6948 | 1.246913225750876 | No Hit |
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT | 5068 | 0.9095216217768334 | TruSeq Adapter, Index 19 (95% over 23bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2866 | 0.5143427324412795 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT | 1616 | 0.29001320852236834 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT | 1432 | 0.2569919026015046 | No Hit |
AAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT | 1328 | 0.23832768621145123 | No Hit |
AATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC | 1229 | 0.22056078791707345 | No Hit |
AACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG | 1142 | 0.2049474530523172 | TruSeq Adapter, Index 13 (95% over 21bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 997 | 0.17892522827772353 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT | 874 | 0.1568512031241027 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTAT | 833 | 0.14949319473956238 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 704 | 0.12634238787113075 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTAACG | 40 | 6.8048394E-9 | 45.000004 | 1 |
AGCGTTG | 20 | 7.0301525E-4 | 45.000004 | 20 |
CTATGCA | 20 | 7.0301525E-4 | 45.000004 | 22 |
AAGTACG | 20 | 7.0301525E-4 | 45.000004 | 1 |
ATCTCGC | 20 | 7.0301525E-4 | 45.000004 | 16 |
TGCCCAT | 20 | 7.0301525E-4 | 45.000004 | 33 |
CTCAATG | 30 | 2.1634805E-6 | 45.000004 | 1 |
CTCAACT | 20 | 7.0301525E-4 | 45.000004 | 30 |
ACGTTAG | 20 | 7.0301525E-4 | 45.000004 | 1 |
TAGACGT | 20 | 7.0301525E-4 | 45.000004 | 28 |
ACCGCAT | 20 | 7.0301525E-4 | 45.000004 | 21 |
TAGGTCG | 30 | 2.1634805E-6 | 45.000004 | 33 |
CGGTTAT | 20 | 7.0301525E-4 | 45.000004 | 6 |
ATTACGG | 55 | 1.8189894E-12 | 45.000004 | 2 |
ATTACCG | 20 | 7.0301525E-4 | 45.000004 | 1 |
GATTTCG | 20 | 7.0301525E-4 | 45.000004 | 35 |
CACCTCG | 30 | 2.1634805E-6 | 45.000004 | 1 |
CCACTTT | 20 | 7.0301525E-4 | 45.000004 | 31 |
GTAGCAA | 20 | 7.0301525E-4 | 45.000004 | 17 |
TTAAGCG | 20 | 7.0301525E-4 | 45.000004 | 25 |