##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557216
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392879960374433	31.0	31.0	34.0	30.0	34.0
2	31.567625480962498	31.0	31.0	34.0	30.0	34.0
3	31.734499727215297	31.0	31.0	34.0	30.0	34.0
4	35.54616522138632	37.0	35.0	37.0	33.0	37.0
5	35.14706325733647	37.0	35.0	37.0	32.0	37.0
6	35.1551301470166	37.0	35.0	37.0	32.0	37.0
7	35.11404912996037	37.0	35.0	37.0	32.0	37.0
8	35.44296825647505	37.0	35.0	37.0	33.0	37.0
9	36.293982943777635	39.0	37.0	39.0	32.0	39.0
10	36.00601562051341	38.0	35.0	39.0	31.0	39.0
11	36.26518082754264	38.0	35.0	39.0	32.0	39.0
12	35.83388667949233	37.0	35.0	39.0	31.0	39.0
13	35.60585123183828	37.0	35.0	39.0	30.0	39.0
14	36.61517975076092	38.0	35.0	40.0	31.0	41.0
15	36.70326408430483	38.0	35.0	40.0	31.0	41.0
16	36.71997035261012	38.0	35.0	40.0	31.0	41.0
17	36.661102696261416	38.0	35.0	40.0	31.0	41.0
18	36.44403965428128	38.0	35.0	40.0	31.0	41.0
19	36.48786646471027	38.0	35.0	40.0	31.0	41.0
20	36.49454968988687	38.0	35.0	40.0	31.0	41.0
21	36.575198845690004	38.0	35.0	40.0	31.0	41.0
22	36.53124461609143	38.0	35.0	40.0	31.0	41.0
23	36.508307370929764	38.0	35.0	40.0	31.0	41.0
24	36.10572381266869	38.0	34.0	40.0	30.0	41.0
25	36.1610434732671	38.0	35.0	40.0	30.0	41.0
26	36.10356307069431	38.0	35.0	40.0	30.0	41.0
27	36.02946792626199	38.0	34.0	40.0	30.0	41.0
28	35.84304650261299	38.0	34.0	40.0	30.0	41.0
29	35.55230287716074	38.0	34.0	40.0	29.0	41.0
30	35.58139572445874	38.0	34.0	40.0	29.0	41.0
31	35.432311706770804	38.0	34.0	40.0	28.0	41.0
32	35.351011098030206	37.0	34.0	40.0	28.0	41.0
33	35.37974142881755	37.0	34.0	40.0	28.0	41.0
34	35.2856701889393	37.0	34.0	40.0	28.0	41.0
35	35.2119232039281	37.0	34.0	40.0	27.0	41.0
36	35.08282784413944	37.0	34.0	40.0	27.0	41.0
37	34.97799058174927	37.0	34.0	40.0	27.0	41.0
38	34.92011356457819	37.0	34.0	40.0	27.0	41.0
39	34.74197618158847	37.0	33.0	40.0	26.0	41.0
40	34.672563242979386	37.0	33.0	40.0	26.0	41.0
41	34.71532045023833	37.0	33.0	40.0	26.0	41.0
42	34.61654367426635	37.0	33.0	40.0	25.0	41.0
43	34.48873506862689	37.0	33.0	40.0	25.0	41.0
44	34.366753646700744	36.0	33.0	40.0	24.0	41.0
45	34.07579466490553	36.0	33.0	40.0	24.0	41.0
46	33.92038993855166	36.0	33.0	39.0	23.0	41.0
47	33.85550127778097	35.0	33.0	39.0	23.0	41.0
48	33.848116349853555	35.0	33.0	39.0	23.0	41.0
49	33.665652099006486	35.0	33.0	39.0	23.0	40.0
50	33.371886306208005	35.0	32.0	39.0	22.0	40.0
51	31.2901101188767	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	8.0
10	5.0
11	8.0
12	9.0
13	10.0
14	23.0
15	56.0
16	102.0
17	200.0
18	441.0
19	861.0
20	1411.0
21	2133.0
22	3050.0
23	3855.0
24	4595.0
25	5511.0
26	6486.0
27	7985.0
28	9549.0
29	12386.0
30	16021.0
31	20595.0
32	26296.0
33	36679.0
34	58215.0
35	62611.0
36	56661.0
37	72982.0
38	87844.0
39	60622.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.13530839028312	36.62206397519095	20.02993453167174	24.21269310285419
2	22.133068684316314	36.52551254809625	18.818555102509617	22.522863665077818
3	19.843471831390342	29.405113995290876	25.383693217710906	25.36772095560788
4	23.389852409119623	30.733683167748232	18.682701142824325	27.193763280307813
5	16.718471831390342	36.93307442715213	18.921028829035777	27.427424912421756
6	20.522023775340262	36.61865416642738	22.541348417848734	20.31797364038362
7	76.68624016539367	10.349666915522885	7.346343249296503	5.6177496697869405
8	78.11979555504507	6.123837075748004	5.7003029345891	10.056064434617815
9	73.06771521277207	10.236245908229485	10.295648366163212	6.400390512835238
10	43.57466404410498	27.084828863492795	13.272052489519325	16.068454602882902
11	34.75833429047264	24.837226497444437	21.79998420720152	18.60445500488141
12	31.945421524148625	22.53560558203641	28.575094756790904	16.94387813702406
13	26.261988169758226	32.385286854648825	21.809495779015677	19.54322919657727
14	17.689728938149656	32.66166082811692	31.141245046804112	18.507365186929306
15	15.995951300752312	27.76697007982542	33.10834577614426	23.12873284327801
16	17.65437460518004	24.810127490955033	34.1481579854132	23.387339918451733
17	16.77590018951358	25.136751277780967	28.142228507436972	29.94512002526848
18	22.346809854706255	25.231149141446046	27.323694940561648	25.098346063286055
19	23.308016998794002	30.202650318727386	25.881525297191754	20.607807385286854
20	24.860197840693736	29.87243725951875	23.46917532877735	21.798189571010166
21	21.88199908114627	28.49343881008442	24.904525354620112	24.7200367541492
22	20.265749727215297	28.593938436800094	24.48296531327169	26.657346522712917
23	23.061792913340607	26.95597398495377	21.636672313788548	28.345560787917073
24	18.586329179348763	32.0157712628496	25.735621374834892	23.662278182966748
25	19.739741859530234	26.910749152931718	25.791434560385916	27.55807442715213
26	22.205213059208635	30.355014931373113	22.568626887957276	24.871145121460977
27	19.597606673175214	28.93743180382473	25.4326867857348	26.03227473726526
28	22.130556193648424	29.637698845690004	26.44432320679952	21.787421753862056
29	20.071211164072817	29.049596565784185	29.23211106644461	21.647081203698388
30	26.485061448343195	26.83268247860794	23.359164130247517	23.323091942801355
31	29.060364382932292	29.101461551714237	20.19539998851433	21.642774076839146
32	26.61624935393097	28.34735542410842	21.357426922414287	23.678968299546316
33	27.034579050134955	26.603686900591512	22.65243639809338	23.709297651180155
34	21.31830385344283	27.556279790960776	26.828195888129557	24.297220467466836
35	26.648911732613566	26.68139464767702	24.786797220467466	21.882896399241947
36	27.104390397978523	24.35787917073451	28.557327858496524	19.980402572790442
37	26.333235226554873	28.84895623959111	22.683483604203754	22.134324929650262
38	27.187123126399815	26.932643714466202	22.54188680870614	23.33834635042784
39	23.04007781542526	25.87344943433067	27.425450812611267	23.661021937632803
40	25.689499224717167	24.59871934761385	22.168064090047665	27.543717337621317
41	21.570450238327687	20.744379199448687	31.602825475219664	26.08234508700396
42	28.686182737035548	23.67286653649572	22.9743941308218	24.66655659564693
43	23.556574111296158	23.10881238155401	26.9985068626888	26.336106644461033
44	22.85415350600126	23.399005053695515	24.995692873140758	28.751148567162467
45	26.968356974674094	26.616608281169242	23.234795842186873	23.180238901969794
46	20.819933383104576	24.750366105783037	32.16634123930397	22.26335927180842
47	23.551549129960375	25.141237868259346	24.648251306495148	26.658961695285132
48	22.185830988342044	21.031879917303165	32.369852983403206	24.41243611095159
49	24.127447883765	20.608884167001666	29.36509360822374	25.89857434100959
50	22.064872509044967	23.000775282834667	26.046272899557803	28.88807930856257
51	20.98378366737495	23.230309251708494	30.00218945615345	25.78371762476311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3295.0
1	2670.0
2	2045.0
3	1408.5
4	772.0
5	700.5
6	629.0
7	654.0
8	679.0
9	729.5
10	780.0
11	848.5
12	917.0
13	1008.0
14	1099.0
15	1152.5
16	1206.0
17	1172.5
18	1139.0
19	1137.5
20	1136.0
21	1039.0
22	942.0
23	1512.5
24	2083.0
25	1956.0
26	2150.0
27	2471.0
28	3427.0
29	4383.0
30	4785.5
31	5188.0
32	6046.5
33	6905.0
34	7116.5
35	7328.0
36	9072.0
37	10816.0
38	11276.0
39	11736.0
40	15751.0
41	19766.0
42	26639.0
43	33512.0
44	47903.5
45	62295.0
46	61993.5
47	61692.0
48	59438.0
49	57184.0
50	52075.5
51	46967.0
52	42815.5
53	38664.0
54	34326.0
55	29988.0
56	28528.0
57	27068.0
58	25086.0
59	23104.0
60	22179.5
61	21255.0
62	20448.0
63	19641.0
64	17778.0
65	15915.0
66	13225.0
67	10535.0
68	8784.0
69	7033.0
70	6452.5
71	5872.0
72	4634.5
73	3397.0
74	2895.5
75	2012.5
76	1631.0
77	1165.0
78	699.0
79	585.5
80	472.0
81	451.5
82	431.0
83	279.5
84	128.0
85	125.0
86	122.0
87	77.5
88	33.0
89	22.5
90	12.0
91	15.5
92	19.0
93	11.0
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.4107832302306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38457207311576	21.75993296920794
2	9.24665382283291	5.06916047085312
3	3.126423808820919	2.5709317592826637
4	1.462965872978505	1.604041616697555
5	0.8060275349603577	1.1046923019197743
6	0.5149461219300855	0.8469045914084081
7	0.3396842517436961	0.6517707973888675
8	0.29539719233559064	0.6477654724743688
9	0.2304411890604712	0.5684916132587847
>10	2.946975990838268	20.240312901161456
>50	1.3304729158777315	26.20695357054051
>100	0.305622652097836	11.191753321964597
>500	0.003272191135951135	0.7096535629949501
>1k	0.0039266293631413625	1.7244438071462223
>5k	0.0026177529087609078	5.1031912437007945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	8523	1.529568425888704	No Hit
AATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	7909	1.4193777637397347	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGT	6948	1.246913225750876	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	5068	0.9095216217768334	TruSeq Adapter, Index 19 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2866	0.5143427324412795	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	1616	0.29001320852236834	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	1432	0.2569919026015046	No Hit
AAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	1328	0.23832768621145123	No Hit
AATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	1229	0.22056078791707345	No Hit
AACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	1142	0.2049474530523172	TruSeq Adapter, Index 13 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	997	0.17892522827772353	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	874	0.1568512031241027	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTAT	833	0.14949319473956238	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	704	0.12634238787113075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7946361913512892E-4	0.0	0.0	1.2079696203985528	0.0
2	1.7946361913512892E-4	0.0	0.0	1.4705248951932464	0.0
3	1.7946361913512892E-4	0.0	0.0	2.0345790501349565	0.0
4	1.7946361913512892E-4	0.0	0.0	5.187395911100902	0.0
5	1.7946361913512892E-4	0.0	0.0	5.727043013840234	0.0
6	1.7946361913512892E-4	0.0	0.0	7.246202549819101	0.0
7	1.7946361913512892E-4	0.0	0.0	8.464760523746627	0.0
8	1.7946361913512892E-4	0.0	0.0	9.130750014357089	0.0
9	1.7946361913512892E-4	0.0	0.0	11.513667949233332	0.0
10	1.7946361913512892E-4	0.0	0.0	13.007882042152415	0.0
11	1.7946361913512892E-4	0.0	0.0	16.190669327513927	0.0
12	1.7946361913512892E-4	0.0	0.0	17.001306495147304	0.0
13	1.7946361913512892E-4	0.0	0.0	17.49554930224545	0.0
14	1.7946361913512892E-4	0.0	0.0	18.02604376040889	0.0
15	1.7946361913512892E-4	0.0	0.0	18.342079193705853	0.0
16	1.7946361913512892E-4	0.0	0.0	18.89069947740194	0.0
17	1.7946361913512892E-4	0.0	0.0	19.480058002641705	1.7946361913512892E-4
18	1.7946361913512892E-4	0.0	0.0	20.392630505943835	1.7946361913512892E-4
19	3.5892723827025784E-4	0.0	0.0	20.72033107448458	1.7946361913512892E-4
20	3.5892723827025784E-4	0.0	0.0	21.091461838856027	1.7946361913512892E-4
21	3.5892723827025784E-4	0.0	0.0	21.4387239418825	1.7946361913512892E-4
22	3.5892723827025784E-4	0.0	0.0	21.797292252914488	1.7946361913512892E-4
23	3.5892723827025784E-4	0.0	0.0	22.206469304542583	1.7946361913512892E-4
24	3.5892723827025784E-4	0.0	0.0	22.47081921552863	1.7946361913512892E-4
25	3.5892723827025784E-4	0.0	0.0	22.708249583644402	1.7946361913512892E-4
26	3.5892723827025784E-4	0.0	0.0	22.907633664503532	1.7946361913512892E-4
27	3.5892723827025784E-4	0.0	0.0	23.146320277953254	1.7946361913512892E-4
28	3.5892723827025784E-4	0.0	0.0	23.36275340263022	1.7946361913512892E-4
29	3.5892723827025784E-4	0.0	0.0	23.578827600068912	1.7946361913512892E-4
30	3.5892723827025784E-4	0.0	0.0	23.92860219376328	1.7946361913512892E-4
31	3.5892723827025784E-4	0.0	0.0	24.159930798828462	1.7946361913512892E-4
32	3.5892723827025784E-4	0.0	0.0	24.396822776086832	1.7946361913512892E-4
33	3.5892723827025784E-4	0.0	0.0	24.65848073278585	1.7946361913512892E-4
34	3.5892723827025784E-4	0.0	0.0	24.884784356515247	1.7946361913512892E-4
35	3.5892723827025784E-4	0.0	0.0	25.15954315741113	1.7946361913512892E-4
36	3.5892723827025784E-4	0.0	0.0	25.386205708378796	1.7946361913512892E-4
37	3.5892723827025784E-4	0.0	0.0	25.624174467351978	1.7946361913512892E-4
38	3.5892723827025784E-4	0.0	0.0	25.85550307241716	1.7946361913512892E-4
39	3.5892723827025784E-4	0.0	0.0	26.094369149486017	1.7946361913512892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	40	6.8048394E-9	45.000004	1
AGCGTTG	20	7.0301525E-4	45.000004	20
CTATGCA	20	7.0301525E-4	45.000004	22
AAGTACG	20	7.0301525E-4	45.000004	1
ATCTCGC	20	7.0301525E-4	45.000004	16
TGCCCAT	20	7.0301525E-4	45.000004	33
CTCAATG	30	2.1634805E-6	45.000004	1
CTCAACT	20	7.0301525E-4	45.000004	30
ACGTTAG	20	7.0301525E-4	45.000004	1
TAGACGT	20	7.0301525E-4	45.000004	28
ACCGCAT	20	7.0301525E-4	45.000004	21
TAGGTCG	30	2.1634805E-6	45.000004	33
CGGTTAT	20	7.0301525E-4	45.000004	6
ATTACGG	55	1.8189894E-12	45.000004	2
ATTACCG	20	7.0301525E-4	45.000004	1
GATTTCG	20	7.0301525E-4	45.000004	35
CACCTCG	30	2.1634805E-6	45.000004	1
CCACTTT	20	7.0301525E-4	45.000004	31
GTAGCAA	20	7.0301525E-4	45.000004	17
TTAAGCG	20	7.0301525E-4	45.000004	25
>>END_MODULE