##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568201
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177929993083435	31.0	31.0	34.0	28.0	34.0
2	31.39192292868193	31.0	31.0	34.0	30.0	34.0
3	31.345708648876013	31.0	31.0	34.0	28.0	34.0
4	35.3382728999069	37.0	35.0	37.0	33.0	37.0
5	35.00138331329934	37.0	35.0	37.0	32.0	37.0
6	35.06450710224023	36.0	35.0	37.0	32.0	37.0
7	34.98043298058258	37.0	35.0	37.0	32.0	37.0
8	35.35483394080616	37.0	35.0	37.0	33.0	37.0
9	35.79074482445473	39.0	37.0	39.0	32.0	39.0
10	35.673872450066085	38.0	35.0	39.0	30.0	39.0
11	36.08200443153039	38.0	35.0	39.0	31.0	39.0
12	35.71808215754636	37.0	35.0	39.0	31.0	39.0
13	35.48508890339862	37.0	35.0	39.0	30.0	39.0
14	36.45909282102636	38.0	35.0	40.0	31.0	41.0
15	36.600701160328825	38.0	35.0	40.0	31.0	41.0
16	36.61275323345084	38.0	35.0	40.0	31.0	41.0
17	36.56586665634168	38.0	35.0	40.0	31.0	41.0
18	36.381094014266075	38.0	35.0	40.0	31.0	41.0
19	36.414927112060695	38.0	35.0	40.0	31.0	41.0
20	36.3970725148319	38.0	35.0	40.0	31.0	41.0
21	36.455263190314696	38.0	35.0	40.0	31.0	41.0
22	36.43799641324109	38.0	35.0	40.0	31.0	41.0
23	36.41074901311332	38.0	35.0	40.0	31.0	41.0
24	35.994202755714966	38.0	34.0	40.0	30.0	41.0
25	36.07472179739212	38.0	34.0	40.0	30.0	41.0
26	36.021453675723905	38.0	35.0	40.0	30.0	41.0
27	35.93406206606465	38.0	34.0	40.0	30.0	41.0
28	35.73938975820176	38.0	34.0	40.0	29.0	41.0
29	35.455602858847485	37.0	34.0	40.0	28.0	41.0
30	35.5253933027221	38.0	34.0	40.0	29.0	41.0
31	35.418402994714896	37.0	34.0	40.0	29.0	41.0
32	35.32625074577482	37.0	34.0	40.0	28.0	41.0
33	35.29458413483961	37.0	34.0	40.0	28.0	41.0
34	35.284568312973754	37.0	34.0	40.0	28.0	41.0
35	35.16969171120783	37.0	34.0	40.0	27.0	41.0
36	35.08668939336608	37.0	34.0	40.0	27.0	41.0
37	34.96311868511319	37.0	34.0	40.0	27.0	41.0
38	34.91286358172548	37.0	34.0	40.0	27.0	41.0
39	34.68957288001957	37.0	33.0	40.0	26.0	41.0
40	34.59725695660514	37.0	33.0	40.0	25.0	41.0
41	34.64112699555263	37.0	33.0	40.0	26.0	41.0
42	34.60163040895739	37.0	33.0	40.0	26.0	41.0
43	34.50621171029266	36.0	33.0	40.0	26.0	41.0
44	34.4788499140269	36.0	33.0	40.0	25.0	41.0
45	34.25102384543498	36.0	33.0	40.0	24.0	41.0
46	33.99429427262535	36.0	33.0	39.0	23.0	41.0
47	33.89463939697396	35.0	33.0	39.0	23.0	41.0
48	33.84242723965639	35.0	33.0	39.0	23.0	40.0
49	33.67447434974595	35.0	33.0	39.0	23.0	40.0
50	33.50748590727577	35.0	33.0	39.0	23.0	40.0
51	31.54649675026971	34.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	11.0
11	7.0
12	8.0
13	19.0
14	32.0
15	41.0
16	91.0
17	242.0
18	460.0
19	847.0
20	1417.0
21	2237.0
22	3117.0
23	3840.0
24	4709.0
25	5551.0
26	6802.0
27	8200.0
28	10187.0
29	13018.0
30	16429.0
31	21495.0
32	27918.0
33	38454.0
34	60838.0
35	64553.0
36	59360.0
37	75263.0
38	86844.0
39	56206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.325731563302423	36.1731147956445	20.18599052096001	24.315163120093068
2	22.288415543091265	36.05942263389188	18.83682006895447	22.81534175406238
3	20.37377618131612	29.347713221201655	24.969157041258285	25.30935355622394
4	23.430089000195352	30.834159038790848	18.66311393327361	27.072638027740183
5	16.84263139276418	36.98849526839974	18.654842212526905	27.514031126309174
6	20.402639206900375	36.80827735255658	22.314814651857354	20.47426878868569
7	77.2691353939891	10.359186273871394	6.926422163987745	5.445256168151763
8	78.74220566313682	6.1729915998035905	5.489782664937231	9.595020072122365
9	72.81349381644876	11.306386296398633	9.670873511310258	6.209246375842352
10	44.07753594238659	27.19794579734988	13.08903011434334	15.635488145920192
11	35.181388276331795	25.103088519731575	21.304960744525264	18.41056245941137
12	32.38906654511344	22.70076962201756	27.880450755982476	17.029713076886523
13	26.185627973199626	32.62173069037189	21.877469416632493	19.315171919795986
14	17.928514733342602	32.9010332611171	30.724338746323927	18.446113259216368
15	16.22700417633901	27.62349943065922	33.316203244978446	22.833293148023323
16	17.939250370907477	24.75057242067508	34.14953511169463	23.160642096722814
17	17.012465659159346	24.995028167849053	28.058556743124353	29.933949429867248
18	22.629140040232244	25.205517061743993	27.336805109459505	24.828537788564258
19	23.568244336071213	29.97460405736702	25.7345552014164	20.722596405145364
20	25.091824899991376	29.859679937205318	23.19584090841093	21.852654254392373
21	22.205522341565747	28.338915278220206	25.054690153660413	24.400872226553634
22	20.53322679826329	28.409840883771764	24.329770626943635	26.727161691021312
23	23.002423438184728	27.084429629655705	21.810943662541955	28.102203269617615
24	18.71362422804606	31.702161735019825	26.00136219401233	23.582851842921784
25	20.05029910190232	26.977777230240708	25.942228190376294	27.02969547748068
26	22.103093799553328	30.416173149994457	22.555750517862517	24.9249825325897
27	19.687927335573153	29.29315506308507	25.386438953820917	25.632478647520863
28	21.97532211312546	29.629831696881915	26.648844335015248	21.746001854977376
29	20.14850378651217	29.20445405763101	29.001884896365898	21.64515725949092
30	26.130013850732396	26.987104915338055	23.65342546035646	23.229455773573086
31	29.009804628995727	29.230853166397104	20.221365326706568	21.537976877900604
32	26.700762582255223	28.233142849097415	21.538152871959042	23.52794169668832
33	26.97196942631217	26.976721265890063	22.64638745795942	23.40492184983835
34	21.36673465903791	27.829588473093146	26.662395877515177	24.141280990353767
35	26.34067873868578	26.710266261411014	25.014739502394402	21.934315497508805
36	27.185802207317483	24.718013519863568	28.127546414033063	19.968637858785886
37	26.18668393755027	28.988333353865976	22.999431539191235	21.825551169392522
38	26.84965355569596	27.152011348096888	22.594469210719446	23.403865885487708
39	23.09922017032705	26.08865524699886	27.265527515791067	23.54659706688302
40	25.72909938560474	24.599745512591493	22.28700759062374	27.384147511180025
41	21.554168331277136	21.107495410954925	31.100262055153017	26.238074202614918
42	28.101675287442298	23.972150700192362	23.157650197729325	24.76852381463602
43	23.526181756103913	23.39594615285788	27.00241639842239	26.07545569261582
44	22.789646621530057	23.677360652304376	25.122799854276916	28.410192871888647
45	26.58988632543765	27.0835496593635	23.184929276787617	23.14163473841123
46	21.06613680722139	25.079857304017416	31.779423126675244	22.07458276208595
47	23.56683638360369	25.449268832684208	24.71572559710384	26.268169186608258
48	22.215554002896862	21.534105008614908	31.917930450667985	24.33241053782024
49	24.144976865581018	21.130550632610642	29.077738335553793	25.646734166254546
50	22.141988486468698	23.574932110291957	25.986754687161763	28.296324716077585
51	20.9668761582609	23.737198632174177	29.70586113012825	25.59006407943668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3613.0
1	2921.0
2	2229.0
3	1514.0
4	799.0
5	693.0
6	587.0
7	688.5
8	790.0
9	791.5
10	793.0
11	838.5
12	884.0
13	992.0
14	1100.0
15	1111.0
16	1122.0
17	1149.5
18	1177.0
19	1173.5
20	1170.0
21	1088.0
22	1006.0
23	1583.0
24	2160.0
25	2037.5
26	2260.0
27	2605.0
28	3609.5
29	4614.0
30	4996.5
31	5379.0
32	6250.5
33	7122.0
34	7482.0
35	7842.0
36	9787.5
37	11733.0
38	11970.0
39	12207.0
40	16435.0
41	20663.0
42	27575.0
43	34487.0
44	48247.5
45	62008.0
46	62084.0
47	62160.0
48	59697.0
49	57234.0
50	52205.5
51	47177.0
52	43099.5
53	39022.0
54	34815.0
55	30608.0
56	29112.5
57	27617.0
58	25809.5
59	24002.0
60	23022.0
61	22042.0
62	21230.5
63	20419.0
64	18419.0
65	16419.0
66	13655.5
67	10892.0
68	9122.0
69	7352.0
70	6677.5
71	6003.0
72	4631.0
73	3259.0
74	2865.0
75	2102.5
76	1734.0
77	1220.0
78	706.0
79	569.5
80	433.0
81	408.0
82	383.0
83	233.5
84	84.0
85	96.5
86	109.0
87	69.0
88	29.0
89	26.0
90	23.0
91	17.0
92	11.0
93	7.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.471663160627564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1224165989377	21.736243772608635
2	9.263413071776185	5.089627272511792
3	3.303471179309878	2.722555424965262
4	1.565638564696472	1.7204278112251954
5	0.8383943989974728	1.151604426250768
6	0.5469414560746124	0.9015234869918957
7	0.33431195557939547	0.6428873803973485
8	0.2647473895446849	0.5818440886581627
9	0.21812473232278806	0.5393024256036317
>10	2.9144188817154264	20.090370066191447
>50	1.2726958218196032	25.161183736133825
>100	0.3451050505187469	12.363955506164599
>500	0.0032252808459695974	0.6207584259662982
>1k	0.005160449353551355	2.7530981746538417
>5k	0.0019351685075817582	3.924618001677307
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	8130	1.4308316951219726	No Hit
AATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	7226	1.271733066291682	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGT	6791	1.1951756508700266	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	4834	0.8507552785017978	TruSeq Adapter, Index 19 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3091	0.5439976346398545	No Hit
AATGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	1596	0.2808865172711769	No Hit
AATGCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTC	1342	0.2361840264272678	No Hit
AATGATCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCT	1313	0.2310801987324908	No Hit
AAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCT	1230	0.21647269188192209	No Hit
AACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTG	1110	0.19535340486905162	TruSeq Adapter, Index 13 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1020	0.1795139396093988	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATGGACTGTCGTAT	797	0.1402672645771479	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTT	796	0.1400912705187073	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	760	0.13375548441484617	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	609	0.10718038159031752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.279821753217611E-4	0.0	0.0	1.0929231029160456	0.0
2	5.279821753217611E-4	0.0	0.0	1.3252352600576205	0.0
3	5.279821753217611E-4	0.0	0.0	1.8331541127171547	0.0
4	5.279821753217611E-4	0.0	0.0	4.622307950883578	0.0
5	5.279821753217611E-4	0.0	0.0	5.14166641734175	0.0
6	5.279821753217611E-4	0.0	0.0	6.549442890808006	0.0
7	5.279821753217611E-4	0.0	0.0	7.652749643172046	0.0
8	5.279821753217611E-4	0.0	0.0	8.275240627876403	0.0
9	5.279821753217611E-4	0.0	0.0	10.494877692929087	0.0
10	5.279821753217611E-4	0.0	0.0	11.872383188343562	0.0
11	5.279821753217611E-4	0.0	0.0	14.87888968868411	0.0
12	5.279821753217611E-4	0.0	0.0	15.669630993257668	0.0
13	5.279821753217611E-4	0.0	0.0	16.112080056177305	0.0
14	5.279821753217611E-4	0.0	0.0	16.630910540460153	0.0
15	5.279821753217611E-4	0.0	0.0	16.907221212211876	0.0
16	5.279821753217611E-4	0.0	0.0	17.435203387533637	0.0
17	5.279821753217611E-4	0.0	0.0	17.98729674886176	0.0
18	5.279821753217611E-4	0.0	0.0	18.87518677369452	0.0
19	5.279821753217611E-4	0.0	0.0	19.19602394223171	0.0
20	5.279821753217611E-4	0.0	0.0	19.533580546320756	0.0
21	5.279821753217611E-4	0.0	0.0	19.889792520604505	0.0
22	5.279821753217611E-4	0.0	0.0	20.244596542420727	0.0
23	5.279821753217611E-4	0.0	0.0	20.63442338186663	0.0
24	5.279821753217611E-4	0.0	0.0	20.879407111215926	0.0
25	5.279821753217611E-4	0.0	0.0	21.09675977339005	0.0
26	5.279821753217611E-4	0.0	0.0	21.298624958421403	0.0
27	5.279821753217611E-4	0.0	0.0	21.524953317576	0.0
28	5.279821753217611E-4	0.0	0.0	21.733858264944974	0.0
29	5.279821753217611E-4	0.0	0.0	21.93924333114514	0.0
30	5.279821753217611E-4	0.0	0.0	22.289119519325027	0.0
31	5.279821753217611E-4	0.0	0.0	22.513335949778334	0.0
32	5.279821753217611E-4	0.0	0.0	22.75444780984194	0.0
33	5.279821753217611E-4	0.0	0.0	22.998903557015915	0.0
34	5.279821753217611E-4	0.0	0.0	23.235087583443182	0.0
35	5.279821753217611E-4	0.0	0.0	23.511574249253346	0.0
36	5.279821753217611E-4	0.0	0.0	23.747758275680614	0.0
37	5.279821753217611E-4	0.0	0.0	23.986230224867608	0.0
38	5.279821753217611E-4	0.0	0.0	24.218542382009183	0.0
39	5.279821753217611E-4	0.0	0.0	24.456662343079298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	25	3.8883063E-5	45.000004	25
CACGGTA	25	3.8883063E-5	45.000004	3
TACCAAT	25	3.8883063E-5	45.000004	38
ACTTGCA	25	3.8883063E-5	45.000004	45
TAGGTCG	25	3.8883063E-5	45.000004	33
CTATAAT	25	3.8883063E-5	45.000004	31
ACTTACG	25	3.8883063E-5	45.000004	1
TCGATAG	25	3.8883063E-5	45.000004	1
CGAATGC	25	3.8883063E-5	45.000004	44
CGGCTAG	25	3.8883063E-5	45.000004	1
CGGTCTA	25	3.8883063E-5	45.000004	31
TAGGCAG	50	2.1827873E-11	45.000004	1
CACGTGA	50	2.1827873E-11	45.000004	43
GTTGGTA	25	3.8883063E-5	45.000004	33
TTATTAG	25	3.8883063E-5	45.000004	17
TATCCTC	25	3.8883063E-5	45.000004	45
GCATAAC	20	7.030272E-4	45.0	30
TCACGGA	20	7.030272E-4	45.0	3
GTCATAG	20	7.030272E-4	45.0	13
TACCCTT	20	7.030272E-4	45.0	43
>>END_MODULE