FastQCFastQC Report
Sat 18 Jun 2016
SRR3548760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398161
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT34890.8762786912831744Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32340.8122342469503543No Hit
AAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT16290.4091309796790746Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT13820.34709577281551934Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC11780.29586021734926327No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT10820.2717493677180839No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGT10080.2531639211273831No Hit
ACCCTTGGGAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTC7860.1974075813552809No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.19087755958016983No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT6100.1532043570314521No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6020.15119511956218717No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5820.14617202588902478No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC5260.1321073636041702Illumina PCR Primer Index 5 (95% over 23bp)
GAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT4710.11829385600297367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGG650.045.0000042
CGGGTTG207.027726E-445.06
CGGGTTA207.027726E-445.06
CTATGTG207.027726E-445.025
AACGTAG207.027726E-445.01
CGGGTAT406.7957444E-945.06
CCTTAGC207.027726E-445.031
GGTACGT207.027726E-445.036
TCGCAAG207.027726E-445.01
ACACGGT207.027726E-445.03
GTATTCG253.8861966E-545.026
GTCGAGC207.027726E-445.029
CTCAACC253.8861966E-545.036
CTATCTC207.027726E-445.07
CATGCGG551.8189894E-1245.02
CGTGCAC253.8861966E-545.035
ACGCACC207.027726E-445.021
GCAACTA207.027726E-445.040
TAGGTTA207.027726E-445.041
TTTCGAT207.027726E-445.013