##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398161
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31643732058137	31.0	31.0	34.0	30.0	34.0
2	31.49433771765693	31.0	31.0	34.0	30.0	34.0
3	31.708645497675562	31.0	31.0	34.0	30.0	34.0
4	35.567230341495026	37.0	35.0	37.0	33.0	37.0
5	35.141927511735204	37.0	35.0	37.0	32.0	37.0
6	35.14093293918792	37.0	35.0	37.0	32.0	37.0
7	35.09651874492982	37.0	35.0	37.0	32.0	37.0
8	35.403369491235956	37.0	35.0	37.0	33.0	37.0
9	36.2175250715163	39.0	37.0	39.0	32.0	39.0
10	35.98264018826555	38.0	35.0	39.0	30.0	39.0
11	36.251370676686065	38.0	35.0	39.0	32.0	39.0
12	35.88450903026665	37.0	35.0	39.0	32.0	39.0
13	35.46886560964032	37.0	35.0	39.0	30.0	39.0
14	36.44770331599529	38.0	35.0	40.0	31.0	41.0
15	36.54925017769194	38.0	35.0	40.0	31.0	41.0
16	36.621788673426074	38.0	35.0	40.0	31.0	41.0
17	36.61352819588056	38.0	35.0	40.0	31.0	41.0
18	36.48842553640362	38.0	35.0	40.0	31.0	41.0
19	36.44409171164428	38.0	35.0	40.0	31.0	41.0
20	36.397854134382825	38.0	35.0	40.0	31.0	41.0
21	36.31984297809178	38.0	35.0	40.0	31.0	41.0
22	36.302000949364704	38.0	35.0	40.0	31.0	41.0
23	36.23023852160307	38.0	35.0	40.0	31.0	41.0
24	35.93110073563207	38.0	34.0	40.0	30.0	41.0
25	35.95912959832832	38.0	34.0	40.0	30.0	41.0
26	35.747501638784314	38.0	34.0	40.0	30.0	41.0
27	35.649938090370476	38.0	34.0	40.0	29.0	41.0
28	35.56998299682792	38.0	34.0	40.0	29.0	41.0
29	35.10424677454598	37.0	34.0	40.0	27.0	41.0
30	35.157913507350045	37.0	34.0	40.0	27.0	41.0
31	34.903614367052526	37.0	34.0	40.0	26.0	41.0
32	34.72303666104917	37.0	34.0	40.0	25.0	41.0
33	34.720954588721646	37.0	33.0	40.0	25.0	41.0
34	34.525603964225525	37.0	33.0	40.0	25.0	41.0
35	34.30371884740092	37.0	33.0	40.0	24.0	41.0
36	34.088913781108644	36.0	33.0	40.0	23.0	41.0
37	33.882919221119096	36.0	33.0	40.0	23.0	41.0
38	33.84077295365443	36.0	33.0	40.0	23.0	41.0
39	33.70986862098498	36.0	33.0	40.0	22.0	41.0
40	33.52617408535743	36.0	32.0	40.0	21.0	41.0
41	33.63687553527342	36.0	33.0	40.0	22.0	41.0
42	33.46793884885762	36.0	32.0	40.0	21.0	41.0
43	33.25147365010636	35.0	32.0	39.0	20.0	41.0
44	33.11889913879059	35.0	32.0	39.0	20.0	41.0
45	32.751633133330486	35.0	31.0	39.0	18.0	40.0
46	32.65368782979749	35.0	31.0	39.0	18.0	40.0
47	32.56544714324105	35.0	31.0	39.0	18.0	40.0
48	32.530506503650535	35.0	31.0	39.0	18.0	40.0
49	32.414709125203125	35.0	31.0	39.0	17.0	40.0
50	32.146624104319606	35.0	31.0	39.0	14.0	40.0
51	30.10596718412903	34.0	26.0	37.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	7.0
12	9.0
13	9.0
14	27.0
15	34.0
16	72.0
17	218.0
18	408.0
19	814.0
20	1422.0
21	2287.0
22	3174.0
23	4207.0
24	5069.0
25	5909.0
26	6675.0
27	7525.0
28	8817.0
29	10492.0
30	12552.0
31	15532.0
32	19464.0
33	25853.0
34	41946.0
35	44960.0
36	36566.0
37	47636.0
38	57152.0
39	39314.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.33775281858344	34.62493815315915	19.511956218715547	25.525352809541868
2	21.50386401480808	33.39302442981608	19.968806588289663	25.13430496708618
3	19.492868462757528	33.33450538852374	20.655714648094616	26.51691150062412
4	20.388737219366035	32.70008865760333	19.162097744379786	27.749076378650848
5	17.0169855912558	36.71002433688885	19.257536524169872	27.015453547685485
6	20.451274735596904	36.916975796223134	20.967899919881656	21.663849548298302
7	76.3404753353543	9.798548828237823	7.910870225863407	5.950105610544478
8	78.70484552731182	7.76494935465804	6.258523562076647	7.271681555953497
9	71.07727778461476	12.16668634045022	11.02569061259139	5.730345262343625
10	42.040782497532405	29.199745831460138	12.06798254977258	16.691489121234877
11	34.48529614904523	26.03745720952077	22.820416866543937	16.656829774890056
12	32.50016952941147	23.65525503502352	27.66443725025806	16.18013818530695
13	23.26043987231296	35.520053445716684	22.98668126712561	18.23282541484475
14	16.37603883856028	37.860312788043025	28.745155854038945	17.018492519357746
15	14.168891478572737	28.162728142635768	38.11699287474163	19.55138750404987
16	14.960279886779468	25.911377558324393	35.64814233438232	23.48020022051381
17	15.357355441642953	25.95909694821944	27.6887992545729	30.99474835556471
18	20.85613608565379	26.054786882693183	27.265352457925314	25.823724573727713
19	22.506975821338603	30.66221955440136	26.576937470018407	20.253867154241625
20	25.70894688329595	26.957687970444116	24.64028370433066	22.69308144192927
21	19.90325521585489	31.26197693897695	25.080055555416024	23.754712289752135
22	18.508342102817704	29.46094670246458	24.483060872360678	27.547650322357036
23	20.701424800520392	31.15724543589151	21.614623230301312	26.526706533286788
24	17.55043813934564	29.334364741900888	27.65012143328955	25.465075685463923
25	19.694545673734996	29.159058772707525	25.913135641110003	25.233259912447476
26	21.47874854644227	32.134990619372566	23.736629152528753	22.649631681656416
27	17.765175393873335	30.907346525651686	25.954576163913597	25.372901916561393
28	20.836797175012116	28.83933886041074	30.44898922797562	19.874874736601527
29	20.862917262112564	28.05046199904059	29.850236462134667	21.236384276712183
30	22.2917362574436	27.378623220255122	26.88987620585642	23.439764316444855
31	26.44332317831229	27.133998558372113	24.212567278060884	22.21011098525471
32	28.225767968233956	25.074027843008228	23.959152202249847	22.74105198650797
33	24.06966026305942	25.41584936746693	26.021132155082995	24.49335821439066
34	21.91199037575252	24.052330589887006	29.37580526470448	24.659873769655995
35	22.897772509110638	23.56885782384513	27.903285354416933	25.630084312627304
36	25.879732068183475	22.454735647137717	32.5707439955194	19.09478828915941
37	20.966895301147023	27.259324745517517	27.975617903310468	23.79816205002499
38	23.003508630930707	26.710802916408188	27.17995986548155	23.10572858717956
39	22.486381137278638	23.987030372135894	28.184076290746706	25.342512199838758
40	24.31880570924827	23.2092043168467	24.68046845371596	27.79152152018907
41	18.629649815024578	21.276820180781144	29.337378598104785	30.7561514060895
42	25.986221654054514	22.270388109332657	25.667255205808704	26.07613503080412
43	20.765469244853215	23.133857911749267	29.03423489492944	27.06643794846808
44	22.434140963077752	23.82101712623788	27.257064353364598	26.48777755731978
45	22.609446932271116	26.67965973563458	26.32728971446224	24.383603617632062
46	21.091719178925107	24.41750949992591	31.12936726600546	23.361404055143524
47	21.584735822945994	23.192879262408926	27.646102958351022	27.576281956294064
48	22.381649634193206	20.87924231655034	33.21344883100053	23.52565921825593
49	21.57971272927283	19.746534693252226	30.90257458666218	27.771177990812763
50	19.470515695911956	24.20000954387798	26.76254078124176	29.566933978968308
51	20.159432993186176	24.231152724651587	29.6425315387494	25.96688274341284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3758.0
1	2965.0
2	2172.0
3	1526.5
4	881.0
5	817.0
6	753.0
7	809.0
8	865.0
9	931.0
10	997.0
11	1026.5
12	1056.0
13	1115.0
14	1174.0
15	1198.5
16	1223.0
17	1203.5
18	1184.0
19	1214.0
20	1244.0
21	1113.5
22	983.0
23	1359.5
24	1736.0
25	1750.0
26	1568.5
27	1373.0
28	2098.0
29	2823.0
30	3233.0
31	3643.0
32	4394.0
33	5145.0
34	5698.5
35	6252.0
36	7131.0
37	8010.0
38	8849.0
39	9688.0
40	13104.0
41	16520.0
42	20582.5
43	24645.0
44	32460.0
45	40275.0
46	41295.0
47	42315.0
48	44005.5
49	45696.0
50	41789.0
51	37882.0
52	33475.5
53	29069.0
54	25338.5
55	21608.0
56	20053.5
57	18499.0
58	16483.5
59	14468.0
60	13872.0
61	13276.0
62	12064.5
63	10853.0
64	9727.5
65	8602.0
66	6923.5
67	5245.0
68	4391.5
69	3538.0
70	3413.0
71	3288.0
72	2726.5
73	2165.0
74	1830.0
75	1268.0
76	1041.0
77	692.5
78	344.0
79	284.0
80	224.0
81	223.5
82	223.0
83	163.5
84	104.0
85	72.5
86	41.0
87	26.0
88	11.0
89	9.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.86472110287851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36561035665086	26.74058091736814
2	8.350908345354622	5.489005474515607
3	2.993101594953098	2.9510234745214325
4	1.3981541317303847	1.8379978239262544
5	0.7734629852077485	1.270982264612625
6	0.49233401003357735	0.9708251957529187
7	0.3654921276644461	0.8408257788692782
8	0.24795987172016565	0.6519305623031022
9	0.19607337076735595	0.5799506981373402
>10	2.5420593156289297	20.384549068831216
>50	1.050544606180539	24.568518531066193
>100	0.2143812885524557	9.48445559589171
>500	0.004577536410372719	0.9693315709339189
>1k	0.005340459145434839	3.260023043270257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	3489	0.8762786912831744	Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3234	0.8122342469503543	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1629	0.4091309796790746	Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1382	0.34709577281551934	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	1178	0.29586021734926327	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1082	0.2717493677180839	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGT	1008	0.2531639211273831	No Hit
ACCCTTGGGAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTC	786	0.1974075813552809	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	760	0.19087755958016983	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	610	0.1532043570314521	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	602	0.15119511956218717	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	582	0.14617202588902478	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	526	0.1321073636041702	Illumina PCR Primer Index 5 (95% over 23bp)
GAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	471	0.11829385600297367	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.511546836581182E-4	0.0	0.0	1.3818530694869664	0.0
2	2.511546836581182E-4	0.0	0.0	1.8150948987972202	0.0
3	2.511546836581182E-4	0.0	0.0	2.3317200830819695	0.0
4	2.511546836581182E-4	0.0	0.0	5.16775877094944	0.0
5	2.511546836581182E-4	0.0	0.0	5.603260992412617	0.0
6	2.511546836581182E-4	0.0	0.0	7.62882351611534	0.0
7	2.511546836581182E-4	0.0	0.0	9.21310726063075	0.0
8	2.511546836581182E-4	0.0	0.0	10.286793533269206	0.0
9	2.511546836581182E-4	0.0	0.0	11.996654619613674	0.0
10	2.511546836581182E-4	0.0	0.0	14.389656445508225	0.0
11	2.511546836581182E-4	0.0	0.0	16.78743021039228	0.0
12	2.511546836581182E-4	0.0	0.0	17.973131471942253	0.0
13	2.511546836581182E-4	0.0	0.0	18.508593257501364	0.0
14	2.511546836581182E-4	0.0	0.0	18.966950555177426	0.0
15	2.511546836581182E-4	0.0	0.0	19.31228824520734	0.0
16	2.511546836581182E-4	0.0	0.0	20.07077538985486	0.0
17	2.511546836581182E-4	0.0	0.0	20.915659745680767	0.0
18	2.511546836581182E-4	0.0	0.0	22.412290505599493	0.0
19	2.511546836581182E-4	0.0	0.0	22.816649546289064	0.0
20	2.511546836581182E-4	0.0	0.0	23.386770678192992	0.0
21	2.511546836581182E-4	0.0	0.0	23.84336989308345	0.0
22	2.511546836581182E-4	0.0	0.0	24.30649912974902	0.0
23	2.511546836581182E-4	0.0	0.0	24.764856427425087	0.0
24	2.511546836581182E-4	0.0	0.0	25.057702788570452	0.0
25	2.511546836581182E-4	0.0	0.0	25.31011324564686	0.0
26	2.511546836581182E-4	0.0	0.0	25.538915162459407	0.0
27	2.511546836581182E-4	0.0	0.0	25.873955510459336	0.0
28	2.511546836581182E-4	0.0	0.0	26.150476817166926	0.0
29	2.511546836581182E-4	0.0	0.0	26.411928842855026	0.0
30	2.511546836581182E-4	0.0	0.0	26.90670356966152	0.0
31	2.511546836581182E-4	0.0	0.0	27.187243351307636	0.0
32	5.023093673162364E-4	0.0	0.0	27.433123786608935	0.0
33	5.023093673162364E-4	0.0	0.0	27.68051115001218	0.0
34	5.023093673162364E-4	0.0	0.0	27.933172761772248	0.0
35	5.023093673162364E-4	0.0	0.0	28.233553763427356	0.0
36	7.534640509743546E-4	0.0	0.0	28.47114609416794	0.0
37	0.0010046187346324728	0.0	0.0	28.725314634029953	0.0
38	0.0010046187346324728	0.0	0.0	28.956125788311763	0.0
39	0.0010046187346324728	0.0	0.0	29.21933589678547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGG	65	0.0	45.000004	2
CGGGTTG	20	7.027726E-4	45.0	6
CGGGTTA	20	7.027726E-4	45.0	6
CTATGTG	20	7.027726E-4	45.0	25
AACGTAG	20	7.027726E-4	45.0	1
CGGGTAT	40	6.7957444E-9	45.0	6
CCTTAGC	20	7.027726E-4	45.0	31
GGTACGT	20	7.027726E-4	45.0	36
TCGCAAG	20	7.027726E-4	45.0	1
ACACGGT	20	7.027726E-4	45.0	3
GTATTCG	25	3.8861966E-5	45.0	26
GTCGAGC	20	7.027726E-4	45.0	29
CTCAACC	25	3.8861966E-5	45.0	36
CTATCTC	20	7.027726E-4	45.0	7
CATGCGG	55	1.8189894E-12	45.0	2
CGTGCAC	25	3.8861966E-5	45.0	35
ACGCACC	20	7.027726E-4	45.0	21
GCAACTA	20	7.027726E-4	45.0	40
TAGGTTA	20	7.027726E-4	45.0	41
TTTCGAT	20	7.027726E-4	45.0	13
>>END_MODULE