SRR3548759_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406650
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3518	0.8651174228451985	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	3353	0.8245419894257962	Illumina PCR Primer Index 5 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1523	0.37452354604696914	Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1371	0.33714496495758023	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	1169	0.28747079798352393	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1052	0.2586991270134022	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGT	1049	0.25796139186032213	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	793	0.19500799213082504	No Hit
ACCCTTGGGAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTC	777	0.19107340464773148	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	653	0.1605803516537563	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	588	0.1445960900036887	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	571	0.14041559080290178	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	433	0.10647977376121971	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	414	0.1018074511250461	Illumina PCR Primer Index 5 (95% over 23bp)
GTTGGTTGATATTGTTCTTCCTATGGGATTGCAAACCCCTTCAGCTCCTTC	409	0.10057789253657937	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	35	1.2093369E-7	45.000004	1
TCCTATA	35	1.2093369E-7	45.000004	15
GACTTAT	35	1.2093369E-7	45.000004	9
AGACGGT	35	1.2093369E-7	45.000004	13
ATATCCG	35	1.2093369E-7	45.000004	1
ATAGTGC	35	1.2093369E-7	45.000004	35
GTAACGG	65	0.0	45.000004	2
TCACGGT	20	7.027904E-4	45.0	3
GAGGATA	20	7.027904E-4	45.0	5
CGGGTAT	40	6.7957444E-9	45.0	6
AACCGCG	20	7.027904E-4	45.0	1
ACTATAT	20	7.027904E-4	45.0	12
GTACCGG	55	1.8189894E-12	45.0	2
TGCCCGC	25	3.8863443E-5	45.0	42
CATATTC	25	3.8863443E-5	45.0	25
ATTAGTC	25	3.8863443E-5	45.0	33
ATTAGTA	25	3.8863443E-5	45.0	12
GTTCTAC	25	3.8863443E-5	45.0	13
GGTAATC	20	7.027904E-4	45.0	8
GTATTCG	25	3.8863443E-5	45.0	26