##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406650
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09654248124923	31.0	31.0	34.0	28.0	34.0
2	31.31222181236936	31.0	31.0	34.0	28.0	34.0
3	31.303627197835976	31.0	31.0	34.0	28.0	34.0
4	35.35741792696422	37.0	35.0	37.0	33.0	37.0
5	34.986037132669374	35.0	35.0	37.0	32.0	37.0
6	35.040836099840156	36.0	35.0	37.0	32.0	37.0
7	34.949428255256365	36.0	35.0	37.0	32.0	37.0
8	35.32332964465757	37.0	35.0	37.0	33.0	37.0
9	35.727431452108696	39.0	35.0	39.0	32.0	39.0
10	35.64721013156277	38.0	35.0	39.0	30.0	39.0
11	36.070028279847534	38.0	35.0	39.0	31.0	39.0
12	35.75890569285627	37.0	35.0	39.0	31.0	39.0
13	35.32529693839911	37.0	35.0	39.0	30.0	39.0
14	36.29633837452354	38.0	35.0	40.0	31.0	41.0
15	36.44873478421247	38.0	35.0	40.0	31.0	41.0
16	36.533534980941845	38.0	35.0	40.0	31.0	41.0
17	36.52808311816058	38.0	35.0	40.0	31.0	41.0
18	36.40731833271855	38.0	35.0	40.0	31.0	41.0
19	36.37226361736137	38.0	35.0	40.0	31.0	41.0
20	36.3036567072421	38.0	35.0	40.0	31.0	41.0
21	36.21009959424567	38.0	34.0	40.0	30.0	41.0
22	36.20855526865855	38.0	34.0	40.0	30.0	41.0
23	36.12669617607279	38.0	34.0	40.0	30.0	41.0
24	35.81406369113488	38.0	34.0	40.0	30.0	41.0
25	35.88890446329768	38.0	34.0	40.0	30.0	41.0
26	35.69948358539284	38.0	34.0	40.0	30.0	41.0
27	35.57824910857002	37.0	34.0	40.0	29.0	41.0
28	35.469130702077955	37.0	34.0	40.0	29.0	41.0
29	35.04250829952047	37.0	34.0	40.0	27.0	41.0
30	35.12098856510513	37.0	34.0	40.0	27.0	41.0
31	34.94377228574942	37.0	34.0	40.0	27.0	41.0
32	34.78252305422353	37.0	34.0	40.0	25.0	41.0
33	34.696739210623385	37.0	33.0	40.0	25.0	41.0
34	34.598986843723104	37.0	33.0	40.0	25.0	41.0
35	34.358799950817655	37.0	33.0	40.0	24.0	41.0
36	34.1723988688061	36.0	33.0	40.0	23.0	41.0
37	33.96663223902619	36.0	33.0	40.0	23.0	41.0
38	33.97704414115333	36.0	33.0	40.0	23.0	41.0
39	33.79954998155662	36.0	33.0	40.0	23.0	41.0
40	33.57748186401082	36.0	32.0	40.0	21.0	41.0
41	33.67309479896717	36.0	33.0	40.0	22.0	41.0
42	33.573089880732816	36.0	33.0	40.0	22.0	41.0
43	33.388242960777085	35.0	32.0	39.0	21.0	41.0
44	33.36302963236198	35.0	32.0	39.0	21.0	41.0
45	33.03606787163408	35.0	32.0	39.0	20.0	41.0
46	32.83684003442764	35.0	31.0	39.0	20.0	40.0
47	32.714103037009714	35.0	31.0	39.0	19.0	40.0
48	32.61587114225993	35.0	31.0	39.0	18.0	40.0
49	32.48961514816181	35.0	31.0	39.0	18.0	40.0
50	32.35965572359523	35.0	31.0	39.0	18.0	40.0
51	30.447669986474857	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	10.0
12	9.0
13	11.0
14	25.0
15	50.0
16	87.0
17	194.0
18	426.0
19	832.0
20	1492.0
21	2270.0
22	3188.0
23	4137.0
24	4864.0
25	5712.0
26	6519.0
27	7761.0
28	8987.0
29	10856.0
30	13050.0
31	16422.0
32	20616.0
33	27911.0
34	43803.0
35	45871.0
36	38403.0
37	49158.0
38	57287.0
39	36679.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.504119021271364	34.52157875322759	19.630148776589206	25.34415344891184
2	21.552932497233492	33.25783843600148	19.93827615885897	25.25095290790606
3	19.786056805606787	33.04512480019673	20.560186892905445	26.608631501291036
4	20.501905815812123	32.874462068117545	19.05643673921062	27.56719537685971
5	17.06430591417681	36.98020410672569	18.84323127997049	27.11225869912701
6	20.397147424074756	37.14078445837944	20.837575310463542	21.62449280708226
7	76.76330997172015	9.932128365916636	7.463912455428501	5.84064920693471
8	79.06258453215295	7.932128365916635	6.069593016107218	6.935694085823189
9	70.72371818517152	13.250215172752982	10.323619820484446	5.702446821591049
10	42.5695315381778	29.20595106356818	12.019427025697775	16.205090372556253
11	35.02471412762818	26.259682773884172	22.18959793434157	16.52600516414607
12	32.95413746465019	23.940981187753597	27.068978236812985	16.035903110783227
13	23.381286118283537	35.78113857125292	22.635681790237307	18.20189352022624
14	16.42296815443256	38.28353621050043	28.435509652034916	16.85798598303209
15	14.220090987335546	28.147792942333705	38.08729865978114	19.544817410549612
16	15.240132792327554	25.85810893889094	35.8630271732448	23.0387310955367
17	15.645395303086193	25.758268781507436	27.4973564490348	31.098979466371574
18	21.05987950325833	26.295585884667403	27.2015246526497	25.443009959424568
19	22.819131931636544	30.44854297307267	26.473134144842003	20.259190950448787
20	25.848272470183204	27.10611090618468	24.346735521947622	22.698881101684496
21	20.121726300258207	30.962498463051762	25.249723349317595	23.666051887372433
22	18.90938153203	29.41743514078446	24.30099594245666	27.37218738472888
23	20.68535595721136	31.247510143858353	21.770564367392105	26.29656953153818
24	17.78949956965449	29.37022009098734	27.72384114103037	25.1164391983278
25	19.66310094676011	29.273084962498462	26.036394934218617	25.027419156522807
26	21.36161318086807	32.31845567441289	23.716709701217262	22.603221443501784
27	17.959424566580598	31.26472396409689	25.90975039960654	24.86610106971597
28	20.394196483462437	29.33898930284028	30.446083855895733	19.82073035780155
29	20.750522562400096	28.34648961022993	29.753842370588956	21.149145456781014
30	22.250829952047216	27.6414607156031	26.845198573712036	23.26251075863765
31	26.626828968400346	27.59916390015984	23.846305176441657	21.927701954998156
32	28.105987950325833	25.59940981187754	23.633099717201524	22.661502520595107
33	24.201401696790853	25.913684987089635	25.866469937292514	24.018443378827
34	21.67588835608017	24.65363334562892	29.254641583671464	24.415836714619452
35	22.992007869174966	24.11385712529202	27.6119513094799	25.282183696053117
36	25.987950325833026	22.944300995942456	31.964834624369853	19.102914053854665
37	21.157752366900283	27.7302348456904	27.653510389770076	23.458502397639247
38	22.88577400713144	27.09086437968769	26.929546292880858	23.093815320300013
39	22.75003073896471	24.356571990655354	27.903848518381903	24.989548751998033
40	24.307389647116683	23.498831919340958	24.56338374523546	27.630394688306897
41	18.74978482724702	21.71646378949957	29.02741915652281	30.506332226730603
42	26.024591171769334	22.484446083855897	25.499569654494035	25.99139308988073
43	20.94454690766015	23.41694331734907	28.920201647608508	26.71830812738227
44	22.321898438460593	24.43772285749416	27.022255010451246	26.218123693594
45	22.37476945776466	27.24775605557605	26.179761465633838	24.197713021025454
46	21.215295708840525	24.78076970367638	30.718554039099967	23.28538054838313
47	21.592770195499817	23.661625476453953	27.34464527234723	27.400959055699005
48	22.433050534857987	21.458256485921552	32.71732448051149	23.391368498708964
49	21.757776958072053	20.31353744005902	30.33173490716833	27.5969506947006
50	19.490716832657075	24.818640108201155	26.677978605680565	29.012664453461205
51	20.21148407721628	24.81027910979958	29.164883806713387	25.813353006270752
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4079.0
1	3209.5
2	2340.0
3	1660.5
4	981.0
5	893.0
6	805.0
7	837.0
8	869.0
9	905.0
10	941.0
11	1004.0
12	1067.0
13	1139.5
14	1212.0
15	1227.0
16	1242.0
17	1238.0
18	1234.0
19	1231.5
20	1229.0
21	1099.0
22	969.0
23	1406.5
24	1844.0
25	1825.0
26	1661.5
27	1517.0
28	2246.0
29	2975.0
30	3377.0
31	3779.0
32	4592.0
33	5405.0
34	6005.0
35	6605.0
36	7565.0
37	8525.0
38	9249.0
39	9973.0
40	13486.0
41	16999.0
42	21157.0
43	25315.0
44	33074.5
45	40834.0
46	41901.0
47	42968.0
48	44295.0
49	45622.0
50	41955.5
51	38289.0
52	33736.5
53	29184.0
54	25693.5
55	22203.0
56	20647.5
57	19092.0
58	17019.0
59	14946.0
60	14354.5
61	13763.0
62	12439.5
63	11116.0
64	9830.5
65	8545.0
66	6949.5
67	5354.0
68	4551.0
69	3748.0
70	3579.0
71	3410.0
72	2845.0
73	2280.0
74	1902.0
75	1306.5
76	1089.0
77	729.0
78	369.0
79	305.0
80	241.0
81	223.0
82	205.0
83	138.5
84	72.0
85	62.0
86	52.0
87	34.0
88	16.0
89	12.0
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	406650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.9707181202171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.04188958978854	26.720092975946745
2	8.607401183082992	5.67584396310105
3	3.0602090829587723	3.0269187318948525
4	1.4728382722024203	1.9424214203781436
5	0.8098754687458933	1.335108789624977
6	0.49566526078580425	0.9805463757211589
7	0.35289785715642724	0.814470704147324
8	0.2506437210059541	0.6611122779111721
9	0.19692470440620716	0.5843474027895718
>10	2.4458437944278453	19.783531469717104
>50	1.012947216774522	23.88395467819072
>100	0.24391291596853365	10.554181751869463
>500	0.0037295552900387407	0.8317428098525579
>1k	0.005221377406054237	3.2057266488551424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3518	0.8651174228451985	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	3353	0.8245419894257962	Illumina PCR Primer Index 5 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1523	0.37452354604696914	Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	1371	0.33714496495758023	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	1169	0.28747079798352393	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1052	0.2586991270134022	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGT	1049	0.25796139186032213	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	793	0.19500799213082504	No Hit
ACCCTTGGGAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTC	777	0.19107340464773148	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	653	0.1605803516537563	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	588	0.1445960900036887	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	571	0.14041559080290178	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	433	0.10647977376121971	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	414	0.1018074511250461	Illumina PCR Primer Index 5 (95% over 23bp)
GTTGGTTGATATTGTTCTTCCTATGGGATTGCAAACCCCTTCAGCTCCTTC	409	0.10057789253657937	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.918234353866962E-4	0.0	0.0	1.2499692610352884	0.0
2	4.918234353866962E-4	0.0	0.0	1.6212959547522439	0.0
3	4.918234353866962E-4	0.0	0.0	2.105496126890446	0.0
4	4.918234353866962E-4	0.0	0.0	4.651666051887372	0.0
5	4.918234353866962E-4	0.0	0.0	5.046354358785196	0.0
6	4.918234353866962E-4	0.0	0.0	6.880363949342186	0.0
7	4.918234353866962E-4	0.0	0.0	8.359031107832289	0.0
8	4.918234353866962E-4	0.0	0.0	9.380548383130456	0.0
9	4.918234353866962E-4	0.0	0.0	11.004303455059633	0.0
10	4.918234353866962E-4	0.0	0.0	13.168080659043403	0.0
11	4.918234353866962E-4	0.0	0.0	15.460961514816182	0.0
12	4.918234353866962E-4	0.0	0.0	16.56412148038854	0.0
13	4.918234353866962E-4	0.0	0.0	17.069224148530676	0.0
14	4.918234353866962E-4	0.0	0.0	17.491454567810155	0.0
15	4.918234353866962E-4	0.0	0.0	17.836468707733925	0.0
16	4.918234353866962E-4	0.0	0.0	18.579859830320913	0.0
17	4.918234353866962E-4	0.0	0.0	19.392843969015125	0.0
18	4.918234353866962E-4	0.0	0.0	20.831181605803515	0.0
19	4.918234353866962E-4	0.0	0.0	21.236444116562154	0.0
20	4.918234353866962E-4	0.0	0.0	21.80154924382147	0.0
21	4.918234353866962E-4	0.0	0.0	22.236075248985614	0.0
22	4.918234353866962E-4	0.0	0.0	22.689290544694455	0.0
23	4.918234353866962E-4	0.0	0.0	23.14299766383868	0.0
24	4.918234353866962E-4	0.0	0.0	23.437845813353007	0.0
25	4.918234353866962E-4	0.0	0.0	23.65178900774622	0.0
26	4.918234353866962E-4	0.0	0.0	23.86351899668019	0.0
27	4.918234353866962E-4	0.0	0.0	24.18664699372925	0.0
28	4.918234353866962E-4	0.0	0.0	24.437476945776467	0.0
29	4.918234353866962E-4	0.0	0.0	24.688552809541374	0.0
30	4.918234353866962E-4	0.0	0.0	25.154309602852575	0.0
31	4.918234353866962E-4	0.0	0.0	25.41915652280831	0.0
32	4.918234353866962E-4	0.0	0.0	25.6672814459609	0.0
33	4.918234353866962E-4	0.0	0.0	25.92302963236198	0.0
34	4.918234353866962E-4	0.0	0.0	26.169187261773022	0.0
35	4.918234353866962E-4	0.0	0.0	26.46551088159351	0.0
36	4.918234353866962E-4	0.0	0.0	26.692241485306774	0.0
37	4.918234353866962E-4	0.0	0.0	26.942579613918603	0.0
38	4.918234353866962E-4	0.0	0.0	27.182835362105003	0.0
39	4.918234353866962E-4	0.0	0.0	27.439321283659165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	35	1.2093369E-7	45.000004	1
TCCTATA	35	1.2093369E-7	45.000004	15
GACTTAT	35	1.2093369E-7	45.000004	9
AGACGGT	35	1.2093369E-7	45.000004	13
ATATCCG	35	1.2093369E-7	45.000004	1
ATAGTGC	35	1.2093369E-7	45.000004	35
GTAACGG	65	0.0	45.000004	2
TCACGGT	20	7.027904E-4	45.0	3
GAGGATA	20	7.027904E-4	45.0	5
CGGGTAT	40	6.7957444E-9	45.0	6
AACCGCG	20	7.027904E-4	45.0	1
ACTATAT	20	7.027904E-4	45.0	12
GTACCGG	55	1.8189894E-12	45.0	2
TGCCCGC	25	3.8863443E-5	45.0	42
CATATTC	25	3.8863443E-5	45.0	25
ATTAGTC	25	3.8863443E-5	45.0	33
ATTAGTA	25	3.8863443E-5	45.0	12
GTTCTAC	25	3.8863443E-5	45.0	13
GGTAATC	20	7.027904E-4	45.0	8
GTATTCG	25	3.8863443E-5	45.0	26
>>END_MODULE