Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548755_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 382240 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT | 2918 | 0.7633947258267058 | Illumina PCR Primer Index 8 (95% over 24bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2839 | 0.7427270824612808 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC | 2478 | 0.6482838007534533 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT | 2022 | 0.5289870238593554 | RNA PCR Primer, Index 40 (95% over 22bp) |
AATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGT | 1988 | 0.5200920887400585 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT | 954 | 0.24958141481791546 | RNA PCR Primer, Index 40 (95% over 22bp) |
AAAAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT | 684 | 0.17894516534114693 | No Hit |
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 682 | 0.17842193386354124 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 628 | 0.16429468396818753 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 552 | 0.1444118878191712 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 480 | 0.12557555462536626 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT | 473 | 0.12374424445374634 | No Hit |
TTAGCGGGGCTTATGAAATTTCAACAAAGAATATGCCTAATTTCTACTCTT | 418 | 0.10935537881958977 | No Hit |
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC | 389 | 0.10176852239430724 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTGACA | 20 | 7.027371E-4 | 45.000004 | 31 |
CTTCGAA | 20 | 7.027371E-4 | 45.000004 | 44 |
GATACGT | 20 | 7.027371E-4 | 45.000004 | 9 |
GATACCC | 20 | 7.027371E-4 | 45.000004 | 43 |
TCTTGCG | 40 | 6.7939254E-9 | 45.000004 | 1 |
GTCGAGG | 20 | 7.027371E-4 | 45.000004 | 34 |
ATCTACG | 20 | 7.027371E-4 | 45.000004 | 1 |
TACCACG | 20 | 7.027371E-4 | 45.000004 | 14 |
CGAAAGT | 20 | 7.027371E-4 | 45.000004 | 43 |
TCTCTCG | 20 | 7.027371E-4 | 45.000004 | 10 |
CCGGCTA | 20 | 7.027371E-4 | 45.000004 | 19 |
ATAATCG | 20 | 7.027371E-4 | 45.000004 | 42 |
TATGGAT | 20 | 7.027371E-4 | 45.000004 | 25 |
TTAGAAT | 20 | 7.027371E-4 | 45.000004 | 29 |
CGTTGGA | 20 | 7.027371E-4 | 45.000004 | 38 |
TAGCGCG | 20 | 7.027371E-4 | 45.000004 | 1 |
CTCCCGA | 20 | 7.027371E-4 | 45.000004 | 25 |
ATAGCGC | 20 | 7.027371E-4 | 45.000004 | 31 |
ACATTCC | 20 | 7.027371E-4 | 45.000004 | 17 |
GCGCCTA | 20 | 7.027371E-4 | 45.000004 | 35 |