FastQCFastQC Report
Sat 18 Jun 2016
SRR3548755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382240
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT29180.7633947258267058Illumina PCR Primer Index 8 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28390.7427270824612808No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC24780.6482838007534533No Hit
AATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT20220.5289870238593554RNA PCR Primer, Index 40 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGT19880.5200920887400585No Hit
AAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT9540.24958141481791546RNA PCR Primer, Index 40 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT6840.17894516534114693No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA6820.17842193386354124No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.16429468396818753No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.1444118878191712No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4800.12557555462536626No Hit
AATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT4730.12374424445374634No Hit
TTAGCGGGGCTTATGAAATTTCAACAAAGAATATGCCTAATTTCTACTCTT4180.10935537881958977No Hit
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC3890.10176852239430724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA207.027371E-445.00000431
CTTCGAA207.027371E-445.00000444
GATACGT207.027371E-445.0000049
GATACCC207.027371E-445.00000443
TCTTGCG406.7939254E-945.0000041
GTCGAGG207.027371E-445.00000434
ATCTACG207.027371E-445.0000041
TACCACG207.027371E-445.00000414
CGAAAGT207.027371E-445.00000443
TCTCTCG207.027371E-445.00000410
CCGGCTA207.027371E-445.00000419
ATAATCG207.027371E-445.00000442
TATGGAT207.027371E-445.00000425
TTAGAAT207.027371E-445.00000429
CGTTGGA207.027371E-445.00000438
TAGCGCG207.027371E-445.0000041
CTCCCGA207.027371E-445.00000425
ATAGCGC207.027371E-445.00000431
ACATTCC207.027371E-445.00000417
GCGCCTA207.027371E-445.00000435