##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382240
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189747279196318	31.0	31.0	34.0	28.0	34.0
2	31.401915027208037	31.0	31.0	34.0	28.0	34.0
3	31.381030242779406	31.0	31.0	34.0	28.0	34.0
4	35.37137662201758	37.0	35.0	37.0	33.0	37.0
5	35.03121337379657	37.0	35.0	37.0	32.0	37.0
6	35.10318647969862	37.0	35.0	37.0	32.0	37.0
7	35.00248534951863	37.0	35.0	37.0	32.0	37.0
8	35.368598262871494	37.0	35.0	37.0	33.0	37.0
9	35.77249372122227	39.0	37.0	39.0	32.0	39.0
10	35.65819118878192	38.0	35.0	39.0	30.0	39.0
11	36.08619715362076	38.0	35.0	39.0	31.0	39.0
12	35.70543637505232	37.0	35.0	39.0	31.0	39.0
13	35.37201496442026	37.0	35.0	39.0	30.0	39.0
14	36.360414922561745	38.0	35.0	40.0	31.0	41.0
15	36.58480797404772	38.0	35.0	40.0	31.0	41.0
16	36.61828170782754	38.0	35.0	40.0	31.0	41.0
17	36.587667434072834	38.0	35.0	40.0	31.0	41.0
18	36.44781812473838	38.0	35.0	40.0	31.0	41.0
19	36.472310590205105	38.0	35.0	40.0	31.0	41.0
20	36.416081519464214	38.0	35.0	40.0	31.0	41.0
21	36.39968344495605	38.0	35.0	40.0	31.0	41.0
22	36.376054311427374	38.0	35.0	40.0	31.0	41.0
23	36.34940613227292	38.0	35.0	40.0	31.0	41.0
24	35.94294683968187	38.0	34.0	40.0	30.0	41.0
25	36.0956284010046	38.0	35.0	40.0	30.0	41.0
26	35.92116994558393	38.0	34.0	40.0	30.0	41.0
27	35.757989744663035	38.0	34.0	40.0	30.0	41.0
28	35.632246755964836	38.0	34.0	40.0	29.0	41.0
29	35.220463583089156	37.0	34.0	40.0	27.0	41.0
30	35.27295939723734	37.0	34.0	40.0	27.0	41.0
31	35.16083612390121	37.0	34.0	40.0	27.0	41.0
32	35.01321682712432	37.0	34.0	40.0	27.0	41.0
33	34.971805671829216	37.0	34.0	40.0	27.0	41.0
34	34.974107890330686	37.0	34.0	40.0	27.0	41.0
35	34.82699089577229	37.0	33.0	40.0	26.0	41.0
36	34.57235768103809	37.0	33.0	40.0	25.0	41.0
37	34.46207356634575	37.0	33.0	40.0	24.0	41.0
38	34.40773336123901	36.0	33.0	40.0	25.0	41.0
39	34.229586123901214	36.0	33.0	40.0	24.0	41.0
40	34.080933444956045	36.0	33.0	40.0	23.0	41.0
41	34.12218501465048	36.0	33.0	40.0	23.0	41.0
42	34.1111893051486	36.0	33.0	40.0	23.0	41.0
43	33.94361134365843	36.0	33.0	40.0	23.0	41.0
44	33.889506592716614	36.0	33.0	40.0	23.0	41.0
45	33.63338216827124	35.0	32.0	39.0	23.0	41.0
46	33.347791963164504	35.0	32.0	39.0	22.0	41.0
47	33.25464367936375	35.0	32.0	39.0	22.0	40.0
48	33.19668271243198	35.0	32.0	39.0	22.0	40.0
49	33.041463478442864	35.0	32.0	39.0	20.0	40.0
50	32.88131802009209	35.0	32.0	39.0	20.0	40.0
51	31.10691188781917	34.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	3.0
12	7.0
13	12.0
14	22.0
15	33.0
16	91.0
17	171.0
18	394.0
19	731.0
20	1111.0
21	1834.0
22	2458.0
23	3229.0
24	3770.0
25	4402.0
26	5269.0
27	6180.0
28	7778.0
29	9675.0
30	11813.0
31	14842.0
32	18911.0
33	25915.0
34	42170.0
35	44095.0
36	36620.0
37	46861.0
38	55611.0
39	38221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.116419003767266	35.06095646714106	20.015173712850565	24.807450816241104
2	22.85553578903307	33.39289451653411	20.122959397237338	23.62861029719548
3	20.44500837170364	33.32906027626622	21.063206362494768	25.162724989535366
4	21.641900376726664	33.56556090414399	19.058706571787358	25.733832147341985
5	17.555724152365006	37.22530347425701	17.847164085391377	27.371808287986603
6	20.168742151527834	38.01695269987442	20.03950397655923	21.77480117203851
7	78.67988698200084	9.46394935119297	6.234826287149435	5.62133737965676
8	80.85182084554206	6.894359564671411	5.145981582251989	7.107838007534534
9	74.06053788195898	12.051067392214316	8.934962327333611	4.953432398493093
10	44.26276684805358	28.976559229803268	11.693961908748431	15.066712013394726
11	35.560904143993305	26.599257011301802	21.53385307660109	16.30598576810381
12	33.51062159899539	25.333821682712433	26.073932607785682	15.081624110506489
13	24.19291544579322	37.16408539137714	20.547300125575553	18.095699037254082
14	15.8546986186689	38.35234407701967	28.66889912097112	17.12405818334031
15	14.935119296776895	28.31100879028882	36.5254813729594	20.228390539974885
16	16.055357890330683	25.705577647551276	36.10558811218083	22.13347634993721
17	15.87510464629552	25.996755964838847	27.51098786102972	30.61715152783591
18	21.915812055253244	26.407492674759315	27.27658015906237	24.40011511092507
19	24.698880284637927	29.8895981582252	25.078223105902055	20.33329845123483
20	27.028830054416076	29.08199037254081	22.08926329007953	21.799916282963583
21	20.689357471745502	31.190351611552952	25.003401004604438	23.11688991209711
22	19.591356215989954	29.91628296358309	24.13405190456258	26.358308915864377
23	21.506121808287986	30.881121808287986	20.687002930096273	26.92575345332775
24	17.912306404353288	30.16638760987861	28.458298451234825	23.46300753453328
25	19.489326077856845	28.186479698618673	26.338687735454165	25.98550648807032
26	20.695374633737966	33.991209711176225	22.520667643365424	22.792748011720384
27	18.613698200083718	32.13321473419841	24.36688991209711	24.886197153620763
28	22.36134365843449	28.953275429049814	29.706728756802008	18.97865215571369
29	19.718763080786943	31.974675596483888	27.582932189200505	20.72362913352867
30	23.40806822938468	31.963949351192966	23.650062787777312	20.97791963164504
31	29.6784742570113	31.24005860192549	18.268365424863962	20.81310171619925
32	26.49984303055672	31.164190037672668	20.774120971117622	21.56184596065299
33	26.36197153620762	30.801852239430723	21.77558601925492	21.060590205106738
34	23.00465676015069	30.023022185014653	21.85223943072415	25.120081624110508
35	22.838792381749688	30.097844286312263	23.99905818334031	23.06430514859774
36	27.807136877354544	29.653882377563832	24.07806613645877	18.460914608622854
37	22.416021347844286	34.591356215989954	20.689619087484303	22.303003348681457
38	23.329845123482627	31.81352030138133	22.654614901632485	22.20201967350356
39	24.05975303474257	29.332095018836334	23.590937630807872	23.017214315613227
40	25.415184177480114	28.69401423189619	21.177532440351612	24.71326915027208
41	20.948618668899122	23.636720385098368	26.722739640016748	28.69192130598577
42	26.307293846797826	26.02501046462955	21.215466722478023	26.452228966094598
43	22.63499372122227	27.15911469233989	24.706467141063207	25.499424445374636
44	22.224257011301802	27.760830891586437	23.723053578903308	26.291858518208457
45	24.14974884889075	30.402365006278774	22.021504813729596	23.426381331100878
46	21.667015487651735	28.49727919631645	27.920416492256173	21.91528882377564
47	22.114116785265804	28.130232314776055	24.44040393470071	25.315246965257433
48	23.863279614901632	22.15519045625785	30.890016743407283	23.091513185433236
49	22.87018627040603	23.260516952699874	28.116366680619503	25.75293009627459
50	20.314985349518626	26.885203013813314	25.34559439095856	27.4542172457095
51	21.458769359564673	26.50821473419841	26.943281707827545	25.089734198409374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3293.0
1	2591.0
2	1889.0
3	1304.0
4	719.0
5	657.0
6	595.0
7	616.0
8	637.0
9	694.5
10	752.0
11	805.0
12	858.0
13	909.0
14	960.0
15	1016.5
16	1073.0
17	1067.0
18	1061.0
19	1080.5
20	1100.0
21	1032.0
22	964.0
23	1111.0
24	1258.0
25	1135.5
26	1358.5
27	1704.0
28	2764.5
29	3825.0
30	4334.5
31	4844.0
32	5841.0
33	6838.0
34	6855.5
35	6873.0
36	7651.5
37	8430.0
38	9392.5
39	10355.0
40	14036.5
41	17718.0
42	21665.5
43	25613.0
44	31756.5
45	37900.0
46	39217.5
47	40535.0
48	39705.5
49	38876.0
50	35611.5
51	32347.0
52	28879.0
53	25411.0
54	23092.0
55	20773.0
56	19118.0
57	17463.0
58	16016.5
59	14570.0
60	14287.5
61	14005.0
62	12679.5
63	11354.0
64	9326.0
65	7298.0
66	6670.5
67	6043.0
68	5463.5
69	4884.0
70	3978.0
71	3072.0
72	2568.0
73	2064.0
74	1714.5
75	1115.5
76	866.0
77	640.0
78	414.0
79	337.5
80	261.0
81	212.5
82	164.0
83	144.0
84	124.0
85	80.0
86	36.0
87	28.5
88	21.0
89	16.5
90	12.0
91	9.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.482592894418538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19149355408646	21.489049851535096
2	9.824459466693204	5.400032398616911
3	3.3725978457187265	2.7806320077144218
4	1.5653950684261335	1.7208446153794348
5	0.8692984168574244	1.1945287247127567
6	0.5282830924201521	0.8711153497192515
7	0.3737758468981798	0.7190630603848431
8	0.26812068800659655	0.589492137204536
9	0.2342316447427403	0.5793563641909322
>10	3.063948751638532	21.312914606533354
>50	1.3645236930902556	26.981566404833206
>100	0.3339046290615308	12.048418935727103
>500	0.004983651180022848	0.9587455955584954
>1k	0.004983651180022848	3.35423994788965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	2918	0.7633947258267058	Illumina PCR Primer Index 8 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2839	0.7427270824612808	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	2478	0.6482838007534533	No Hit
AATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	2022	0.5289870238593554	RNA PCR Primer, Index 40 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGT	1988	0.5200920887400585	No Hit
AAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	954	0.24958141481791546	RNA PCR Primer, Index 40 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	684	0.17894516534114693	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	682	0.17842193386354124	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	628	0.16429468396818753	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	552	0.1444118878191712	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	480	0.12557555462536626	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	473	0.12374424445374634	No Hit
TTAGCGGGGCTTATGAAATTTCAACAAAGAATATGCCTAATTTCTACTCTT	418	0.10935537881958977	No Hit
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	389	0.10176852239430724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1246860611134366	0.0
2	0.0	0.0	0.0	1.370604855588112	0.0
3	0.0	0.0	0.0	1.752302218501465	0.0
4	0.0	0.0	0.0	3.901998744244454	0.0
5	0.0	0.0	0.0	4.215676015069066	0.0
6	0.0	0.0	0.0	5.679677689409795	0.0
7	0.0	0.0	0.0	6.785265801590624	0.0
8	0.0	0.0	0.0	7.581100879028883	0.0
9	0.0	0.0	0.0	9.201025533696107	0.0
10	0.0	0.0	0.0	10.717088740058601	0.0
11	0.0	0.0	0.0	12.92093972373378	0.0
12	0.0	0.0	0.0	13.75104646295521	0.0
13	0.0	0.0	0.0	14.21410632063625	0.0
14	0.0	0.0	0.0	14.670625784847216	0.0
15	0.0	0.0	0.0	14.961804102134785	0.0
16	0.0	0.0	0.0	15.507796149016325	0.0
17	0.0	0.0	0.0	16.142999162829636	0.0
18	0.0	0.0	0.0	17.160946002511512	0.0
19	0.0	0.0	0.0	17.49241314357472	0.0
20	0.0	0.0	0.0	17.833821682712433	0.0
21	0.0	0.0	0.0	18.19380493930515	0.0
22	0.0	0.0	0.0	18.57994976977815	0.0
23	0.0	0.0	0.0	18.95667643365425	0.0
24	0.0	0.0	0.0	19.20678107994977	0.0
25	0.0	0.0	0.0	19.410579740477186	0.0
26	0.0	0.0	0.0	19.62196525742989	0.0
27	0.0	0.0	0.0	19.871023440770198	0.0
28	0.0	0.0	0.0	20.08110087902888	0.0
29	0.0	0.0	0.0	20.31341565508581	0.0
30	0.0	0.0	0.0	20.697205943909587	0.0
31	0.0	0.0	0.0	20.942601506906655	0.0
32	0.0	0.0	0.0	21.17857890330682	0.0
33	0.0	0.0	0.0	21.419788614483046	0.0
34	0.0	0.0	0.0	21.662044788614484	0.0
35	0.0	0.0	0.0	21.929416073670993	0.0
36	0.0	0.0	0.0	22.149958141481793	0.0
37	0.0	0.0	0.0	22.361866889912097	0.0
38	0.0	0.0	0.0	22.582932189200502	0.0
39	0.0	0.0	0.0	22.795625784847218	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACA	20	7.027371E-4	45.000004	31
CTTCGAA	20	7.027371E-4	45.000004	44
GATACGT	20	7.027371E-4	45.000004	9
GATACCC	20	7.027371E-4	45.000004	43
TCTTGCG	40	6.7939254E-9	45.000004	1
GTCGAGG	20	7.027371E-4	45.000004	34
ATCTACG	20	7.027371E-4	45.000004	1
TACCACG	20	7.027371E-4	45.000004	14
CGAAAGT	20	7.027371E-4	45.000004	43
TCTCTCG	20	7.027371E-4	45.000004	10
CCGGCTA	20	7.027371E-4	45.000004	19
ATAATCG	20	7.027371E-4	45.000004	42
TATGGAT	20	7.027371E-4	45.000004	25
TTAGAAT	20	7.027371E-4	45.000004	29
CGTTGGA	20	7.027371E-4	45.000004	38
TAGCGCG	20	7.027371E-4	45.000004	1
CTCCCGA	20	7.027371E-4	45.000004	25
ATAGCGC	20	7.027371E-4	45.000004	31
ACATTCC	20	7.027371E-4	45.000004	17
GCGCCTA	20	7.027371E-4	45.000004	35
>>END_MODULE