FastQCFastQC Report
Sat 18 Jun 2016
SRR3548753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563999
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC107971.914365096392015No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGT101541.8003578020528406No Hit
AATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT79431.408335830382678No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT48130.8533703073941621TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27610.48953987507070046No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT17000.30141897414711727No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT15890.2817380881880996No Hit
TAGGGGGGCTGGAAAGATGGCTCAGAGGTTAAGAACACTGACTACTTTTCC11970.21223441885535257No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTAT11560.20496490242003976No Hit
AACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG11370.20159610212074844No Hit
AAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT10720.19007125899159397No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8120.14397188647497602No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.12074489493775699No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT5920.10496472511476085No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGG5900.1046101145569407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATA207.030225E-445.00000415
CTTAACG207.030225E-445.0000041
AGCGTCG406.8048394E-945.0000041
CCTTACT207.030225E-445.00000417
AGTCCCG207.030225E-445.00000440
CATATGG351.2104647E-745.0000042
ACGCCCG207.030225E-445.00000423
TACTTTA207.030225E-445.00000428
CTTCCGT207.030225E-445.00000442
ACGTTTA207.030225E-445.00000416
GAATGCG207.030225E-445.0000041
AACCCAT207.030225E-445.00000418
TAGCGCG207.030225E-445.0000041
GCACGTC207.030225E-445.00000437
TAAACCG207.030225E-445.0000041
ATTCGTT207.030225E-445.00000417
ATTAACG700.045.0000041
GATCACG207.030225E-445.0000041
ATAGACG351.2104647E-745.0000041
CTAAGCG406.8048394E-945.0000041