##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563999
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189062391954597	31.0	31.0	34.0	28.0	34.0
2	31.403232984455645	31.0	31.0	34.0	30.0	34.0
3	31.33859989113456	31.0	31.0	34.0	28.0	34.0
4	35.31675233466726	37.0	35.0	37.0	33.0	37.0
5	34.967561999223406	35.0	35.0	37.0	32.0	37.0
6	35.0364716958718	36.0	35.0	37.0	32.0	37.0
7	34.88172674065025	36.0	35.0	37.0	32.0	37.0
8	35.24486213628038	37.0	35.0	37.0	33.0	37.0
9	35.77374427968844	39.0	35.0	39.0	32.0	39.0
10	35.70686827458914	37.0	35.0	39.0	30.0	39.0
11	36.10925551286439	37.0	35.0	39.0	32.0	39.0
12	35.65614832650412	37.0	35.0	39.0	31.0	39.0
13	35.49903279970354	37.0	35.0	39.0	30.0	39.0
14	36.419738332869386	38.0	35.0	40.0	31.0	41.0
15	36.590242181280466	38.0	35.0	40.0	31.0	41.0
16	36.53711088140227	38.0	35.0	40.0	31.0	41.0
17	36.5141356633611	38.0	35.0	40.0	31.0	41.0
18	36.29790301046633	38.0	35.0	40.0	30.0	41.0
19	36.351647786609554	38.0	35.0	40.0	30.0	41.0
20	36.34488536327192	38.0	35.0	40.0	31.0	41.0
21	36.43185714868289	38.0	35.0	40.0	31.0	41.0
22	36.39898297692017	38.0	35.0	40.0	31.0	41.0
23	36.33997755315169	38.0	35.0	40.0	31.0	41.0
24	35.98615068466434	38.0	34.0	40.0	30.0	41.0
25	36.06240081985961	38.0	34.0	40.0	30.0	41.0
26	35.99837942975076	38.0	34.0	40.0	30.0	41.0
27	35.95398396096447	38.0	34.0	40.0	30.0	41.0
28	35.79471240197234	38.0	34.0	40.0	30.0	41.0
29	35.5310222181245	37.0	34.0	40.0	29.0	41.0
30	35.64146744941037	38.0	34.0	40.0	29.0	41.0
31	35.445803981921955	37.0	34.0	40.0	29.0	41.0
32	35.33856797618436	37.0	34.0	40.0	29.0	41.0
33	35.32634632330908	37.0	34.0	40.0	29.0	41.0
34	34.96607972709172	37.0	34.0	40.0	26.0	41.0
35	34.98953189633315	37.0	34.0	40.0	26.0	41.0
36	34.886434195805315	37.0	33.0	40.0	26.0	41.0
37	34.395424459972446	37.0	33.0	40.0	25.0	41.0
38	34.423108906221465	37.0	33.0	40.0	25.0	41.0
39	33.79362020145426	36.0	32.0	40.0	23.0	41.0
40	33.6415117757301	36.0	32.0	39.0	22.0	41.0
41	33.628976292511155	36.0	32.0	40.0	22.0	41.0
42	33.57843010359947	36.0	32.0	40.0	22.0	41.0
43	33.59104714724671	35.0	33.0	39.0	22.0	41.0
44	33.40912483887383	35.0	32.0	39.0	21.0	41.0
45	33.00905852669951	35.0	32.0	39.0	20.0	41.0
46	32.85025859974929	35.0	31.0	39.0	19.0	40.0
47	32.56190347855227	35.0	31.0	39.0	18.0	40.0
48	32.537458399748935	35.0	31.0	39.0	18.0	40.0
49	32.50075266090897	35.0	31.0	39.0	18.0	40.0
50	32.22220429468847	35.0	31.0	39.0	15.0	40.0
51	30.35271161828301	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	3.0
11	6.0
12	9.0
13	15.0
14	13.0
15	46.0
16	91.0
17	204.0
18	412.0
19	816.0
20	1440.0
21	2262.0
22	3229.0
23	4280.0
24	5748.0
25	7130.0
26	8710.0
27	10635.0
28	13002.0
29	15740.0
30	19049.0
31	23281.0
32	29243.0
33	38884.0
34	61117.0
35	63257.0
36	54367.0
37	68208.0
38	80388.0
39	52406.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.005351073317506	38.61531669382393	19.326807317034252	24.052524915824318
2	21.9778758472976	38.37418151450623	19.24808377319818	20.399858864997988
3	19.808191149275086	28.366539656985207	27.308913668286642	24.516355525453058
4	26.311571474417505	29.062817487265047	17.894535273998713	26.731075764318728
5	16.571837893329597	39.101487768595334	18.314748785015574	26.01192555305949
6	21.31156260915356	32.36229142250252	25.460506135649176	20.86563983269474
7	72.92690235266375	13.254810735480028	7.796822334791374	6.021464577064853
8	75.68665901889898	5.746996005312067	5.481215392225873	13.08512958356309
9	71.85952457362514	10.56101163299935	10.426436926306607	7.153026867068914
10	42.302557274037724	27.11866510401614	14.103925716180349	16.474851905765792
11	35.501304080326385	23.323445608946113	21.94755664460398	19.22769366612352
12	31.209629804308165	22.33195448928101	30.515302332096333	15.943113374314494
13	25.479655105771464	34.21318122904473	20.396667369977607	19.910496295206197
14	15.96332617611024	34.08428029127711	32.55342651316758	17.39896701944507
15	14.453217115633185	28.44969583279403	32.12807114906232	24.969015902510467
16	16.16226269904734	24.95997333328605	36.245454335911944	22.632309631754666
17	16.11155338927906	25.014937969748175	28.539412303922525	30.33409633705024
18	19.78585068413242	26.46848664625292	26.448096539178263	27.2975661304364
19	22.021138335351658	28.73834882685962	29.102356564461996	20.138156273326725
20	25.657314995239354	30.27203948943172	23.022558550635726	21.048086964693198
21	21.228583738623648	28.059269608634057	24.829831258566063	25.88231539417623
22	19.2202468444093	31.164062347628274	23.64738235351481	25.968308454447612
23	22.633196158149214	27.898276415383716	19.632127007317386	29.836400419149676
24	17.335669034874176	33.51867645155399	25.67203133338889	23.473623180182944
25	19.312445589442532	24.631249346186785	28.682852274560773	27.37345278980991
26	23.43302027131254	30.022925572563068	22.35730914416515	24.18674501195924
27	17.3941797769145	27.908028205723767	27.715651978106344	26.982140039255388
28	21.925038874182402	26.973452080588796	29.11334949175442	21.988159553474386
29	21.32947044232348	28.73196583681886	28.04473057576343	21.89383314509423
30	24.61954719777872	24.524334263004015	24.503412240092622	26.352706299124645
31	30.27806786891466	28.26760331135339	20.800746100613654	20.653582719118297
32	25.051817467761467	22.9269910053032	25.959443190502114	26.061748336433222
33	30.037109994875877	22.97504073588783	24.9291222147557	22.058727054480592
34	23.21138867267495	23.46369408456398	28.48054695132438	24.844370291436686
35	22.15837262122805	22.04968448525618	31.87257424215291	23.919368651362856
36	30.79012551440694	23.433729492428178	26.87433842967807	18.901806563486815
37	23.167771574063075	25.704123588871614	25.793840060000107	25.334264777065208
38	29.260690178528687	24.84685256534143	25.298271805446465	20.594185450683423
39	21.795960631135873	24.29436931625765	31.747219409963495	22.162450642642984
40	26.825934088535618	23.552524029297924	27.118487798737235	22.503054083429223
41	20.487447672779563	20.038865317137088	33.995273041264255	25.47841396881909
42	25.270434876657582	21.983726921501635	27.508559412339384	25.2372787895014
43	23.97521981421953	21.730889593775878	28.68852604348589	25.605364548518704
44	21.464577064852953	22.286564337880034	27.231431261402943	29.01742733586407
45	27.306431394381903	25.709265441960007	24.523270431330552	22.461032732327542
46	20.653760024397204	23.231956085028518	34.88250865693024	21.231775233644033
47	23.66812707114729	25.225222030535516	25.476463610751082	25.630187287566113
48	21.564222631600412	19.21740995994674	34.85378520174681	24.36458220670604
49	23.636212120943476	19.033721690995904	31.553602045393696	25.77646414266692
50	21.0516330702714	22.883196601412415	26.535330736402013	29.52983959191417
51	20.04773058108259	21.901279966808453	32.44899370388954	25.601995748219412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3214.0
1	2567.0
2	1920.0
3	1318.5
4	717.0
5	711.5
6	706.0
7	728.5
8	751.0
9	841.0
10	931.0
11	975.0
12	1019.0
13	1073.5
14	1128.0
15	1226.0
16	1324.0
17	1333.5
18	1343.0
19	1571.5
20	1800.0
21	1604.5
22	1409.0
23	1453.0
24	1497.0
25	1627.0
26	2130.0
27	2503.0
28	3232.0
29	3961.0
30	4599.5
31	5238.0
32	5358.5
33	5479.0
34	7392.0
35	9305.0
36	9708.0
37	10111.0
38	11150.0
39	12189.0
40	16223.5
41	20258.0
42	26476.0
43	32694.0
44	50152.5
45	67611.0
46	68868.0
47	70125.0
48	64675.0
49	59225.0
50	53513.5
51	47802.0
52	44642.0
53	41482.0
54	37738.0
55	33994.0
56	30795.5
57	27597.0
58	25009.5
59	22422.0
60	20897.0
61	19372.0
62	17069.5
63	14767.0
64	13154.5
65	11542.0
66	9735.0
67	7928.0
68	6794.5
69	5661.0
70	5122.0
71	4583.0
72	3698.5
73	2814.0
74	2724.5
75	2119.5
76	1604.0
77	1208.0
78	812.0
79	605.0
80	398.0
81	284.5
82	171.0
83	140.5
84	110.0
85	77.5
86	45.0
87	31.5
88	18.0
89	13.5
90	9.0
91	10.0
92	11.0
93	7.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.049321042677022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.24304139669714	22.507628295767805
2	8.843827725709213	4.961267262490917
3	3.122826430793528	2.627794833536546
4	1.4502124719547493	1.6270990082381205
5	0.853276506777519	1.1966913338388305
6	0.547184031782692	0.920888434613947
7	0.3963199110888162	0.7781553095214794
8	0.259138654211954	0.5814930645246693
9	0.2208797550961928	0.5575974446268889
>10	2.5686820241467965	18.01737633030478
>50	1.054982277404929	21.192202246831805
>100	0.42694111406705776	16.195805638279197
>500	0.005709465121253373	0.9498473982853318
>1k	0.005075080107780776	2.744735128990492
>5k	6.34385013472597E-4	1.4133828933271622
>10k+	0.001268770026945194	3.728035376822029
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	10797	1.914365096392015	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGT	10154	1.8003578020528406	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	7943	1.408335830382678	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	4813	0.8533703073941621	TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2761	0.48953987507070046	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	1700	0.30141897414711727	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	1589	0.2817380881880996	No Hit
TAGGGGGGCTGGAAAGATGGCTCAGAGGTTAAGAACACTGACTACTTTTCC	1197	0.21223441885535257	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTAT	1156	0.20496490242003976	No Hit
AACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTG	1137	0.20159610212074844	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1072	0.19007125899159397	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	812	0.14397188647497602	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.12074489493775699	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	592	0.10496472511476085	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGG	590	0.1046101145569407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.77305278910069E-4	0.0	0.0	1.3726974693217542	0.0
2	1.77305278910069E-4	0.0	0.0	1.6466341252378107	0.0
3	1.77305278910069E-4	0.0	0.0	2.279791276225667	0.0
4	1.77305278910069E-4	0.0	0.0	5.879443048657888	0.0
5	1.77305278910069E-4	0.0	0.0	6.241323122913339	0.0
6	1.77305278910069E-4	0.0	0.0	7.83653871726723	0.0
7	1.77305278910069E-4	0.0	0.0	9.133526832494384	0.0
8	1.77305278910069E-4	0.0	0.0	9.874840203617381	0.0
9	1.77305278910069E-4	0.0	0.0	13.301087413275555	0.0
10	1.77305278910069E-4	0.0	0.0	14.75091267892319	0.0
11	1.77305278910069E-4	0.0	0.0	18.73726726465827	0.0
12	1.77305278910069E-4	0.0	0.0	19.561027590474453	0.0
13	1.77305278910069E-4	0.0	0.0	20.002340429681613	0.0
14	1.77305278910069E-4	0.0	0.0	20.626986927281784	0.0
15	1.77305278910069E-4	0.0	0.0	20.933193143959475	0.0
16	1.77305278910069E-4	0.0	0.0	21.480889150512677	0.0
17	1.77305278910069E-4	0.0	0.0	22.071847645119938	0.0
18	1.77305278910069E-4	0.0	0.0	23.15075026728771	0.0
19	1.77305278910069E-4	0.0	0.0	23.491531013352862	0.0
20	1.77305278910069E-4	0.0	0.0	23.855184140397412	0.0
21	1.77305278910069E-4	0.0	0.0	24.18709962251706	0.0
22	1.77305278910069E-4	0.0	0.0	24.525220789398563	0.0
23	1.77305278910069E-4	0.0	0.0	24.881427094728892	0.0
24	1.77305278910069E-4	0.0	0.0	25.125576463788057	0.0
25	1.77305278910069E-4	0.0	0.0	25.326463344793165	0.0
26	1.77305278910069E-4	0.0	0.0	25.532669384165576	0.0
27	1.77305278910069E-4	0.0	0.0	25.772563426530898	0.0
28	1.77305278910069E-4	0.0	0.0	25.97930138174004	0.0
29	1.77305278910069E-4	0.0	0.0	26.200932980377626	0.0
30	1.77305278910069E-4	0.0	0.0	26.57841591917716	0.0
31	1.77305278910069E-4	0.0	0.0	26.800579433651478	0.0
32	1.77305278910069E-4	0.0	0.0	27.021324505894516	0.0
33	1.77305278910069E-4	0.0	0.0	27.255899389892534	0.0
34	1.77305278910069E-4	0.0	0.0	27.48231823106069	0.0
35	1.77305278910069E-4	0.0	0.0	27.733737116555172	0.0
36	1.77305278910069E-4	0.0	0.0	27.947744588199626	0.0
37	1.77305278910069E-4	0.0	0.0	28.170617323789582	0.0
38	1.77305278910069E-4	0.0	0.0	28.380724079298012	0.0
39	3.54610557820138E-4	0.0	0.0	28.608029446860723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCATA	20	7.030225E-4	45.000004	15
CTTAACG	20	7.030225E-4	45.000004	1
AGCGTCG	40	6.8048394E-9	45.000004	1
CCTTACT	20	7.030225E-4	45.000004	17
AGTCCCG	20	7.030225E-4	45.000004	40
CATATGG	35	1.2104647E-7	45.000004	2
ACGCCCG	20	7.030225E-4	45.000004	23
TACTTTA	20	7.030225E-4	45.000004	28
CTTCCGT	20	7.030225E-4	45.000004	42
ACGTTTA	20	7.030225E-4	45.000004	16
GAATGCG	20	7.030225E-4	45.000004	1
AACCCAT	20	7.030225E-4	45.000004	18
TAGCGCG	20	7.030225E-4	45.000004	1
GCACGTC	20	7.030225E-4	45.000004	37
TAAACCG	20	7.030225E-4	45.000004	1
ATTCGTT	20	7.030225E-4	45.000004	17
ATTAACG	70	0.0	45.000004	1
GATCACG	20	7.030225E-4	45.000004	1
ATAGACG	35	1.2104647E-7	45.000004	1
CTAAGCG	40	6.8048394E-9	45.000004	1
>>END_MODULE