FastQCFastQC Report
Sat 18 Jun 2016
SRR3548752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435099
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC96312.2135192220621054No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT86411.9859847988618684No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT84491.9418569107260646No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT44751.0285015594152136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25850.5941176605783971No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT19150.4401297176044992No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT15720.36129708411189176No Hit
AACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG11760.2702833148317969No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT11380.2615496703049191No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTAT8930.20524064638162812No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT7330.16846740626845844No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC6670.15329844472177595No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6470.14870178970762976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC302.16238E-645.00000443
CAAACGT302.16238E-645.00000441
GTACACT351.2095916E-745.00000418
CAAAACG302.16238E-645.0000041
TAAACCG302.16238E-645.0000041
CATTCTC351.2095916E-745.00000426
ACGTGCA302.16238E-645.00000444
ACCACGC302.16238E-645.00000425
CCGGTGT302.16238E-645.00000437
ATTATCG302.16238E-645.00000421
TGGGACG600.045.0000046
GCTAACG351.2095916E-745.0000041
ATCGCGG302.16238E-645.0000042
CCTTGCG302.16238E-645.0000041
ATCGCAA302.16238E-645.00000424
GCGTAAG302.16238E-645.0000041
TACAATG302.16238E-645.0000041
CACCACG302.16238E-645.00000424
TCTGCGT253.8867947E-545.023
GTTGAAA207.028447E-445.023
Produced by FastQC (version 0.11.5)