##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435099
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373041537673036	31.0	31.0	34.0	30.0	34.0
2	31.556820401793615	31.0	31.0	34.0	30.0	34.0
3	31.721674837220956	31.0	31.0	34.0	30.0	34.0
4	35.50338658558167	37.0	35.0	37.0	33.0	37.0
5	35.08285010997497	37.0	35.0	37.0	32.0	37.0
6	35.08860742038019	37.0	35.0	37.0	32.0	37.0
7	35.0177752649397	37.0	35.0	37.0	32.0	37.0
8	35.38051340039853	37.0	35.0	37.0	33.0	37.0
9	36.30886533869303	39.0	37.0	39.0	32.0	39.0
10	35.99516202059761	38.0	35.0	39.0	31.0	39.0
11	36.27449844747977	38.0	35.0	39.0	32.0	39.0
12	35.75871238499744	37.0	35.0	39.0	31.0	39.0
13	35.529456514494406	37.0	35.0	39.0	30.0	39.0
14	36.485487210956585	38.0	35.0	40.0	31.0	41.0
15	36.60049781773803	38.0	35.0	40.0	31.0	41.0
16	36.58579771500279	38.0	35.0	40.0	31.0	41.0
17	36.54041264172062	38.0	35.0	40.0	31.0	41.0
18	36.2759394988267	38.0	35.0	40.0	30.0	41.0
19	36.32263691711542	38.0	35.0	40.0	30.0	41.0
20	36.35907230308504	38.0	35.0	40.0	31.0	41.0
21	36.472782056497486	38.0	35.0	40.0	31.0	41.0
22	36.433515131039144	38.0	35.0	40.0	31.0	41.0
23	36.39146952762475	38.0	35.0	40.0	31.0	41.0
24	36.064566914656204	38.0	34.0	40.0	30.0	41.0
25	36.1354335450093	38.0	35.0	40.0	30.0	41.0
26	36.05604241793247	38.0	35.0	40.0	30.0	41.0
27	36.00860493818648	38.0	34.0	40.0	30.0	41.0
28	35.93455052758108	38.0	34.0	40.0	30.0	41.0
29	35.73862500258562	38.0	34.0	40.0	30.0	41.0
30	35.83897227987194	38.0	34.0	40.0	30.0	41.0
31	35.48759018062556	37.0	34.0	40.0	29.0	41.0
32	35.60545301184328	38.0	34.0	40.0	30.0	41.0
33	35.549491035373556	38.0	34.0	40.0	29.0	41.0
34	35.26565218490504	37.0	34.0	40.0	28.0	41.0
35	35.358116198842104	37.0	34.0	40.0	29.0	41.0
36	35.12486353680427	37.0	34.0	40.0	27.0	41.0
37	35.11518757799949	37.0	34.0	40.0	27.0	41.0
38	35.03430713469808	37.0	34.0	40.0	27.0	41.0
39	34.901073089113055	37.0	34.0	40.0	27.0	41.0
40	34.6976757013921	37.0	33.0	40.0	26.0	41.0
41	34.68800893589735	37.0	33.0	40.0	26.0	41.0
42	34.80077637503189	37.0	34.0	40.0	26.0	41.0
43	34.81750819928338	37.0	34.0	40.0	27.0	41.0
44	34.64638162808924	37.0	33.0	40.0	26.0	41.0
45	34.36145337038237	36.0	33.0	40.0	25.0	41.0
46	34.27013852019885	36.0	33.0	39.0	24.0	41.0
47	34.12545190864608	36.0	33.0	39.0	24.0	41.0
48	34.14207111484972	36.0	33.0	39.0	24.0	41.0
49	33.99622154957837	36.0	33.0	39.0	24.0	41.0
50	33.6857198017003	35.0	33.0	39.0	23.0	40.0
51	31.639417695742807	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	9.0
11	5.0
12	9.0
13	12.0
14	22.0
15	51.0
16	68.0
17	173.0
18	290.0
19	566.0
20	936.0
21	1432.0
22	2081.0
23	2616.0
24	3301.0
25	3956.0
26	4632.0
27	5697.0
28	7325.0
29	9462.0
30	12106.0
31	16082.0
32	21262.0
33	29288.0
34	47775.0
35	51818.0
36	43653.0
37	55518.0
38	67766.0
39	47183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.09703308902112	38.738080298966445	18.27170368123117	23.893182930781272
2	20.12576448118704	38.2085456413368	18.77411807427735	22.89157180319881
3	19.279290460332017	29.704044366914196	27.168989126612566	23.847676046141224
4	25.721042797156507	30.732316093578703	17.498546307851775	26.048094801413015
5	16.801693407707212	38.98308201122043	18.475335498357843	25.739889082714505
6	19.989243827266897	34.91481249095034	24.421338591906668	20.674605089876096
7	73.79010294208904	12.558291331398141	8.050351759024958	5.601253967487859
8	76.1339373338022	5.709735025821709	5.721226663357075	12.435100977019022
9	72.20816411897063	9.959572419150584	10.753414740093634	7.078848721785157
10	41.90379660720893	28.538562488077424	13.325243220508437	16.232397684205203
11	34.967903856363726	22.88651548268325	24.073371807335803	18.072208853617223
12	32.04443126736673	22.290329327348488	30.805632741054335	14.85960666423044
13	24.745402770403977	34.8915993831289	20.795497116748145	19.567500729718983
14	15.831799199722363	34.45284866202864	32.09016798475749	17.625184153491507
15	13.774106582639813	28.701284075578204	33.86010999795449	23.664499343827497
16	16.39098228219325	24.301365896037453	37.43929542471943	21.86835639704987
17	16.370527167380295	25.69162420506597	28.612798466555887	29.32505016099784
18	19.391219009926477	27.385721410529555	27.37537893674773	25.847680642796238
19	21.838707972208624	29.855274316879605	29.217258600916114	19.088759109995657
20	25.581304484726463	30.70243783598675	24.142551465298702	19.573706213988082
21	20.054286495717065	29.91227283905502	25.316766988662348	24.716673676565563
22	18.989471361690097	31.568677473402605	24.459490828524082	24.982360336383213
23	21.568884322878244	28.585448369221716	21.17587031916874	28.6697969887313
24	17.50750978512936	33.042365070937876	26.959151825216786	22.49097331871597
25	18.410982328159797	25.503391182236683	28.766786409529786	27.31884008007373
26	22.797110542658107	31.812759854653766	21.939374717018424	23.450754885669696
27	16.54818788367705	29.168534057766166	28.05131705657793	26.23196100197886
28	19.91569734704056	27.967198269819054	30.610045070202414	21.507059312937976
29	21.194027106474618	29.240012043236135	27.109002778677954	22.45695807161129
30	24.4820144380934	25.294703044594446	24.114282036961704	26.109000480350446
31	26.926285741865648	32.74680015352828	19.307559888669015	21.019354215937064
32	25.006492775207484	23.81205196978159	22.105084130278396	29.076371124732532
33	28.680828960765254	25.859861778583728	24.318373519589795	21.140935741061227
34	21.24872730114296	29.19542448959892	23.410074488794503	26.145773720463616
35	22.024642667530838	23.007637342306005	30.888832196810377	24.078887793352777
36	27.189214408674804	28.268509005996336	24.92559164695851	19.616684938370348
37	19.823074748505512	29.533968131390786	21.54590104780751	29.09705607229619
38	24.547286939294274	30.186922976150253	24.060271340545487	21.205518744009986
39	21.60243990448151	28.162785940670975	24.68036010195381	25.55441405289371
40	26.301370492692467	26.41214987853339	23.73252983803686	23.55394979073728
41	18.828128770693567	23.90766239407583	31.56890730615331	25.69530152907729
42	25.510975663010026	23.683345629385496	24.933635793233265	25.872042914371214
43	23.273783667625068	23.389619373981553	26.487075355263972	26.8495216031294
44	20.860309952447604	23.995458504846024	25.427086709001863	29.717144833704513
45	26.453519773660705	28.323209200664678	23.2464335702909	21.976837455383716
46	19.42247626402267	24.667719300664906	32.99524935704288	22.914555078269544
47	22.498557799489312	26.88629484324257	24.891576399853825	25.72357095741429
48	21.186902291202692	20.30434452848662	34.540644772798835	23.968108407511853
49	23.268957179860216	20.04210535992958	30.460653782242662	26.228283677967546
50	20.413515085072593	24.69874672201039	24.760801564701367	30.126936628215645
51	19.239989059961065	22.827448465751473	31.38274277808039	26.549819696207074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2971.0
1	2331.0
2	1691.0
3	1178.0
4	665.0
5	602.5
6	540.0
7	584.0
8	628.0
9	690.0
10	752.0
11	807.5
12	863.0
13	947.5
14	1032.0
15	1070.5
16	1109.0
17	1050.0
18	991.0
19	1002.5
20	1014.0
21	943.5
22	873.0
23	1005.5
24	1138.0
25	1508.5
26	2257.5
27	2636.0
28	3065.0
29	3494.0
30	4097.0
31	4700.0
32	4808.0
33	4916.0
34	5991.5
35	7067.0
36	7443.0
37	7819.0
38	8847.5
39	9876.0
40	12389.0
41	14902.0
42	20962.0
43	27022.0
44	43742.5
45	60463.0
46	56950.0
47	53437.0
48	49184.0
49	44931.0
50	41824.5
51	38718.0
52	34451.5
53	30185.0
54	27225.5
55	24266.0
56	20825.5
57	17385.0
58	16667.0
59	15949.0
60	14896.5
61	13844.0
62	12735.5
63	11627.0
64	10529.5
65	9432.0
66	7708.5
67	5985.0
68	4780.5
69	3576.0
70	2896.0
71	2216.0
72	1957.5
73	1699.0
74	1433.0
75	941.0
76	715.0
77	536.0
78	357.0
79	300.0
80	243.0
81	181.0
82	119.0
83	90.5
84	62.0
85	48.0
86	34.0
87	63.5
88	93.0
89	53.5
90	14.0
91	8.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	435099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.330888942552267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13052894479677	21.627076985556585
2	8.982440510979002	4.909961680772991
3	3.1502901866171724	2.583006936817386
4	1.4901558505017647	1.6290913622863303
5	0.880405472384803	1.2031132095082162
6	0.5741879824796703	0.9415840788780012
7	0.3866972121856212	0.7398144992447848
8	0.31355813433257124	0.6855858037181912
9	0.24126279570732284	0.5934534007901865
>10	3.38356213208139	22.834735117214038
>50	1.2063134477565554	23.297228732347058
>100	0.24966905504090375	9.185772348871982
>500	0.003362546195837088	0.6754751183232942
>1k	0.005043819293755632	2.954859012502002
>5k	0.002521909646877816	6.1392417131689605
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCC	9631	2.2135192220621054	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGT	8641	1.9859847988618684	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	8449	1.9418569107260646	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	4475	1.0285015594152136	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2585	0.5941176605783971	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	1915	0.4401297176044992	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCT	1572	0.36129708411189176	No Hit
AACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTG	1176	0.2702833148317969	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCT	1138	0.2615496703049191	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCACGATTCGTAT	893	0.20524064638162812	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTT	733	0.16846740626845844	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC	667	0.15329844472177595	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	647	0.14870178970762976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.596655014146206E-4	0.0	0.0	1.4017499465638854	0.0
2	4.596655014146206E-4	0.0	0.0	1.7037501809932911	0.0
3	4.596655014146206E-4	0.0	0.0	2.393708098616637	0.0
4	4.596655014146206E-4	0.0	0.0	6.249152491731767	0.0
5	4.596655014146206E-4	0.0	0.0	6.662851443004925	0.0
6	4.596655014146206E-4	0.0	0.0	8.459224222533262	0.0
7	4.596655014146206E-4	0.0	0.0	9.868788482621197	0.0
8	4.596655014146206E-4	0.0	0.0	10.696875883419635	0.0
9	4.596655014146206E-4	0.0	0.0	14.003249835095001	0.0
10	4.596655014146206E-4	0.0	0.0	15.665859953711683	0.0
11	4.596655014146206E-4	0.0	0.0	19.751137097534123	0.0
12	4.596655014146206E-4	0.0	0.0	20.63760201701222	0.0
13	4.596655014146206E-4	0.0	0.0	21.12277895375535	0.0
14	4.596655014146206E-4	0.0	0.0	21.71942477459153	0.0
15	4.596655014146206E-4	0.0	0.0	22.053141928618544	0.0
16	4.596655014146206E-4	0.0	0.0	22.607728356075285	0.0
17	4.596655014146206E-4	0.0	0.0	23.23540159825695	0.0
18	4.596655014146206E-4	0.0	0.0	24.254250182142457	0.0
19	4.596655014146206E-4	0.0	0.0	24.600378304707665	0.0
20	4.596655014146206E-4	0.0	0.0	24.96650187658441	0.0
21	4.596655014146206E-4	0.0	0.0	25.312859831900326	0.0
22	4.596655014146206E-4	0.0	0.0	25.68863637930678	0.0
23	6.894982521219308E-4	0.0	0.0	26.085557539778303	0.0
24	6.894982521219308E-4	0.0	0.0	26.348256373836758	0.0
25	6.894982521219308E-4	0.0	0.0	26.585213939815997	0.0
26	6.894982521219308E-4	0.0	0.0	26.80010756172733	0.0
27	6.894982521219308E-4	0.0	0.0	27.05154459100113	0.0
28	6.894982521219308E-4	0.0	0.0	27.27287352993227	0.0
29	6.894982521219308E-4	0.0	0.0	27.49673062912119	0.0
30	6.894982521219308E-4	0.0	0.0	27.904913594377373	0.0
31	6.894982521219308E-4	0.0	0.0	28.156120790900463	0.0
32	6.894982521219308E-4	0.0	0.0	28.387102705361308	0.0
33	6.894982521219308E-4	0.0	0.0	28.61854428532357	0.0
34	6.894982521219308E-4	0.0	0.0	28.86032833906766	0.0
35	6.894982521219308E-4	0.0	0.0	29.12095867836975	0.0
36	6.894982521219308E-4	0.0	0.0	29.34205778455018	0.0
37	6.894982521219308E-4	0.0	0.0	29.5783258522773	0.0
38	6.894982521219308E-4	0.0	0.0	29.860790302896582	0.0
39	6.894982521219308E-4	0.0	0.0	30.107400844405525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGC	30	2.16238E-6	45.000004	43
CAAACGT	30	2.16238E-6	45.000004	41
GTACACT	35	1.2095916E-7	45.000004	18
CAAAACG	30	2.16238E-6	45.000004	1
TAAACCG	30	2.16238E-6	45.000004	1
CATTCTC	35	1.2095916E-7	45.000004	26
ACGTGCA	30	2.16238E-6	45.000004	44
ACCACGC	30	2.16238E-6	45.000004	25
CCGGTGT	30	2.16238E-6	45.000004	37
ATTATCG	30	2.16238E-6	45.000004	21
TGGGACG	60	0.0	45.000004	6
GCTAACG	35	1.2095916E-7	45.000004	1
ATCGCGG	30	2.16238E-6	45.000004	2
CCTTGCG	30	2.16238E-6	45.000004	1
ATCGCAA	30	2.16238E-6	45.000004	24
GCGTAAG	30	2.16238E-6	45.000004	1
TACAATG	30	2.16238E-6	45.000004	1
CACCACG	30	2.16238E-6	45.000004	24
TCTGCGT	25	3.8867947E-5	45.0	23
GTTGAAA	20	7.028447E-4	45.0	23
>>END_MODULE