FastQCFastQC Report
Sat 18 Jun 2016
SRR3548748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638129
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT44860.702992655090115No Hit
AATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT38980.6108482767590879No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC34510.5407997442523377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30620.47984028307755955No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGT29780.46667680045884136No Hit
AAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT13790.21610050632395644No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT9800.15357396388504518No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8950.14025377313991372No Hit
AATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC8880.13915681625502055No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7250.11361339164965077No Hit
AATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT6710.10515115282333197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTC302.1639771E-645.00000415
AACGGTA302.1639771E-645.0000044
CGTAGCA302.1639771E-645.00000432
CGAGGTT302.1639771E-645.00000435
CACTACG351.2107921E-745.0000041
CGCTAAG351.2107921E-745.0000041
GAACGTA302.1639771E-645.00000429
CTCGATG351.2107921E-745.0000041
CGGTATC351.2107921E-745.0000046
AATAGCG351.2107921E-745.0000041
CGCAAAT351.2107921E-745.0000041
GATTACG351.2107921E-745.0000041
GCGTAGC351.2107921E-745.0000041
TAACTGC351.2107921E-745.00000437
ACGGTAT351.2107921E-745.0000045
GTGTTAC253.8888484E-545.035
CGGAACG502.1827873E-1145.01
GTCGCAT207.030926E-445.030
CATATCG207.030926E-445.01
GTCGACT253.8888484E-545.024