##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656115
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198416436143056	31.0	31.0	34.0	28.0	34.0
2	31.39498563514018	31.0	31.0	34.0	28.0	34.0
3	31.34588905908263	31.0	31.0	34.0	28.0	34.0
4	35.37416306592594	37.0	35.0	37.0	33.0	37.0
5	35.0567903492528	37.0	35.0	37.0	32.0	37.0
6	35.117445874579914	37.0	35.0	37.0	32.0	37.0
7	35.07868132873048	37.0	35.0	37.0	32.0	37.0
8	35.43892305464743	37.0	35.0	37.0	33.0	37.0
9	35.89052528901183	39.0	37.0	39.0	32.0	39.0
10	35.65499188404472	38.0	35.0	39.0	30.0	39.0
11	36.0745265692752	38.0	35.0	39.0	31.0	39.0
12	35.796840492901396	37.0	35.0	39.0	31.0	39.0
13	35.53399632686343	37.0	35.0	39.0	30.0	39.0
14	36.55101925729483	38.0	35.0	40.0	31.0	41.0
15	36.679169048108946	38.0	35.0	40.0	31.0	41.0
16	36.70787895414676	38.0	35.0	40.0	31.0	41.0
17	36.65120748649246	38.0	35.0	40.0	31.0	41.0
18	36.50227627778667	38.0	35.0	40.0	31.0	41.0
19	36.56762762625454	38.0	35.0	40.0	31.0	41.0
20	36.54112465040427	38.0	35.0	40.0	31.0	41.0
21	36.519529350799786	38.0	35.0	40.0	31.0	41.0
22	36.485564268459036	38.0	35.0	40.0	31.0	41.0
23	36.44358839532704	38.0	35.0	40.0	31.0	41.0
24	36.12068006370834	38.0	34.0	40.0	30.0	41.0
25	36.186695929829376	38.0	35.0	40.0	30.0	41.0
26	36.112798823377	38.0	35.0	40.0	30.0	41.0
27	36.000365789533845	38.0	34.0	40.0	30.0	41.0
28	35.76699206693949	38.0	34.0	40.0	29.0	41.0
29	35.33645930972467	38.0	34.0	40.0	27.0	41.0
30	35.38511389009549	38.0	34.0	40.0	27.0	41.0
31	35.24057825228809	37.0	34.0	40.0	27.0	41.0
32	35.23386906258811	37.0	34.0	40.0	27.0	41.0
33	35.150568116869756	37.0	34.0	40.0	27.0	41.0
34	35.14027723798419	37.0	34.0	40.0	27.0	41.0
35	34.94981062771008	37.0	34.0	40.0	26.0	41.0
36	34.74896016704389	37.0	33.0	40.0	25.0	41.0
37	34.64623274883214	37.0	33.0	40.0	25.0	41.0
38	34.589120809614165	37.0	33.0	40.0	25.0	41.0
39	34.45125321018419	37.0	33.0	40.0	24.0	41.0
40	34.32964038316454	37.0	33.0	40.0	24.0	41.0
41	34.34473834617407	37.0	33.0	40.0	24.0	41.0
42	34.317006927139296	36.0	33.0	40.0	24.0	41.0
43	34.153927284088915	36.0	33.0	40.0	23.0	41.0
44	34.170834381167936	36.0	33.0	40.0	24.0	41.0
45	33.97477576339514	36.0	33.0	40.0	23.0	41.0
46	33.71166487582207	35.0	32.0	39.0	23.0	41.0
47	33.64347561022077	35.0	32.0	39.0	23.0	41.0
48	33.60051972596268	35.0	32.0	39.0	23.0	41.0
49	33.31495698162669	35.0	32.0	39.0	22.0	40.0
50	33.17192565327725	35.0	32.0	39.0	21.0	40.0
51	31.418358062229945	34.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	14.0
11	17.0
12	15.0
13	29.0
14	42.0
15	81.0
16	120.0
17	262.0
18	549.0
19	987.0
20	1720.0
21	2736.0
22	3912.0
23	5025.0
24	6063.0
25	7146.0
26	8519.0
27	10511.0
28	12737.0
29	15552.0
30	19536.0
31	24997.0
32	32223.0
33	43696.0
34	68867.0
35	71901.0
36	65775.0
37	83678.0
38	100755.0
39	68643.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.63024774620303	33.349946274662216	19.71026420673205	27.309541772402703
2	24.670522698002635	32.175761871013464	20.07071930987708	23.08299612110682
3	20.890087865694277	31.208858203211324	21.433437735762787	26.467616195331612
4	21.610236010455484	32.83037272429376	19.22406895132713	26.335322313923626
5	18.093322054822707	35.46832491255344	17.945329705920457	28.493023326703398
6	20.823788512684512	36.9214238357605	20.958825815596352	21.295961835958636
7	81.3682052688934	7.803205230790335	5.952462601830471	4.876126898485784
8	82.78061010645999	5.991022915190173	5.091485486538183	6.136881491811649
9	75.67575806070582	11.267232116321072	8.194295207395045	4.862714615578062
10	40.7878192085229	31.76882101460872	12.610441767068274	14.83291800980011
11	34.484351066505106	28.268824824916365	21.307849995808663	15.938974112769866
12	32.41626848951784	24.495248546367634	27.408152534235615	15.680330429878907
13	26.790425458951557	33.19037059052148	21.750607744069256	18.26859620645771
14	17.56003139693499	36.172469765208845	28.404624189357047	17.86287464849912
15	16.831043338439144	27.178924426358186	34.678219519444	21.31181271575867
16	18.304413098313557	26.910526355897975	32.464583190446795	22.32047735534167
17	18.47983966225433	25.824283852678263	26.886597623892154	28.80927886117525
18	25.192077608346096	24.66930339955648	26.52461839768943	23.61400059440799
19	25.649466937960568	28.407672435472442	23.970340565297242	21.972520061269744
20	27.21596061666019	27.562546199980186	23.708343811679356	21.51314937168027
21	23.842923877673883	30.09502907264733	24.22380222979203	21.838244819886757
22	22.449570578328494	29.357048688111075	23.619335025109926	24.5740457084505
23	22.49422738391898	30.14181965051858	21.677297424994094	25.686655540568342
24	19.743490089389816	28.809583685786787	27.241413471723707	24.205512753099686
25	20.41959107778362	27.64301989742652	25.4479778697332	26.489411155056658
26	22.60609801635384	31.1513987639362	22.1766001386952	24.06590308101476
27	19.750653467760987	29.801482971735137	24.36280225265388	26.085061307849994
28	22.900406178794878	29.396523475305397	27.4127249034087	20.290345442491027
29	19.80475983630918	31.284759531484575	26.551290551199102	22.359190081007142
30	24.69094594697576	28.48204964068799	24.608033652637115	22.21897075969914
31	26.377997759539106	31.920471258849442	19.543372731914374	22.15815824969708
32	24.44053252859636	30.236162867789947	21.922833649588867	23.400470954024826
33	24.011187063243487	28.678813927436504	22.543761383294086	24.766237626025926
34	22.0644246816488	28.34045860862806	23.949764904018352	25.64535180570479
35	21.16351554224488	31.28445470687303	24.716856038956585	22.835173711925503
36	23.101438010104935	31.518864833146626	24.547373554940826	20.83232360180761
37	20.529937587160788	34.43786531324539	20.113851992409867	24.918345107183953
38	20.752611965890125	32.032341891284304	22.19046965852023	25.024576484305346
39	22.80057611851581	26.276338751590806	23.086958841056827	27.83612628883656
40	25.57524214505079	26.116763067450066	21.15315150545255	27.154843282046592
41	19.475854080458458	24.511861487696518	26.011598576469062	30.000685855375963
42	26.240064622817645	26.770916683813052	21.677297424994094	25.31172126837521
43	21.343057238441432	28.09690374400829	24.209475473049693	26.35056354450058
44	22.710957682723304	26.420825617460352	23.341639803997776	27.526576895818565
45	23.32959923184198	28.088825891802504	22.978898516266202	25.602676360089315
46	19.76665675986679	28.049960753831265	27.451894865991477	24.731487620310464
47	20.376305982945063	27.943424552098335	24.049290139685876	27.630979325270722
48	21.465596732280165	24.505460170854196	29.27169779688012	24.75724529998552
49	24.4303209041098	23.747818598873675	27.346120725787397	24.47573977122913
50	20.763890476517073	27.11658779329843	24.640192649154493	27.479329081030002
51	20.777455171730566	25.289011834815543	27.810216196855738	26.123316796598157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3976.0
1	3115.0
2	2254.0
3	1555.5
4	857.0
5	746.5
6	636.0
7	736.0
8	836.0
9	932.5
10	1029.0
11	1068.0
12	1107.0
13	1217.5
14	1328.0
15	1392.5
16	1457.0
17	1462.0
18	1467.0
19	1537.0
20	1607.0
21	1469.5
22	1332.0
23	1724.5
24	2117.0
25	2785.0
26	3439.0
27	3425.0
28	4126.5
29	4828.0
30	5924.0
31	7020.0
32	8055.5
33	9091.0
34	11046.0
35	13001.0
36	14699.0
37	16397.0
38	18444.0
39	20491.0
40	23155.5
41	25820.0
42	32019.0
43	38218.0
44	48668.0
45	59118.0
46	61552.0
47	63986.0
48	64346.5
49	64707.0
50	60996.5
51	57286.0
52	51451.5
53	45617.0
54	43390.5
55	41164.0
56	37146.5
57	33129.0
58	32217.0
59	31305.0
60	29387.5
61	27470.0
62	25519.5
63	23569.0
64	19084.5
65	14600.0
66	12381.0
67	10162.0
68	8707.5
69	7253.0
70	5911.0
71	4569.0
72	4047.5
73	3526.0
74	3208.5
75	2355.0
76	1819.0
77	1394.0
78	969.0
79	803.5
80	638.0
81	497.5
82	357.0
83	244.5
84	132.0
85	83.5
86	35.0
87	25.5
88	16.0
89	18.5
90	21.0
91	14.5
92	8.0
93	7.5
94	7.0
95	9.5
96	12.0
97	6.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.41963493046138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57106771529486	23.409517632109967
2	9.309979764617191	5.477924117700412
3	3.2303537546484136	2.8510748447400465
4	1.5312408146082401	1.801941830255869
5	0.8517425415589421	1.2528977313703704
6	0.5342484543013342	0.9430436692625119
7	0.3307652942836892	0.6811695943844932
8	0.25193959741014055	0.5929576784266999
9	0.21155772576398474	0.5601555952825586
>10	2.664381961361584	19.42834684156487
>50	1.0964103133778678	23.33972215537396
>100	0.40795448058509287	15.693561622562994
>500	0.005223488867926925	1.0855470295798693
>1k	0.003134093320756155	2.8821396573853972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	4411	0.6722906807495637	No Hit
AATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	3547	0.5406064485646571	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3458	0.5270417533511655	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC	3187	0.4857380184876127	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGT	2825	0.4305647637990291	No Hit
AAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	1327	0.20225112975621654	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT	943	0.14372480434070245	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	935	0.14250550589454594	No Hit
AATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	933	0.1422006812830068	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	792	0.12071054616949771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9210275637654984	0.0
2	0.0	0.0	0.0	1.1702216836987418	0.0
3	0.0	0.0	0.0	1.5244278823072175	0.0
4	0.0	0.0	0.0	3.456863507159568	0.0
5	0.0	0.0	0.0	3.870967741935484	0.0
6	0.0	0.0	0.0	5.108555664784375	0.0
7	0.0	0.0	0.0	6.077135867949978	0.0
8	0.0	0.0	0.0	6.692119521730184	0.0
9	0.0	0.0	0.0	8.010028729719638	0.0
10	0.0	0.0	0.0	9.3529335558553	0.0
11	0.0	0.0	0.0	11.278053390030712	0.0
12	0.0	0.0	0.0	12.079437293767098	0.0
13	0.0	0.0	0.0	12.61150865320866	0.0
14	0.0	0.0	0.0	13.06691662284813	0.0
15	0.0	0.0	0.0	13.352842108471839	0.0
16	0.0	0.0	0.0	13.903507769217287	0.0
17	0.0	0.0	0.0	14.490142734124353	0.0
18	0.0	0.0	0.0	15.378401652149394	0.0
19	0.0	0.0	0.0	15.68368350060584	0.0
20	0.0	0.0	0.0	16.064866677335527	0.0
21	0.0	0.0	0.0	16.438429238776738	0.0
22	0.0	0.0	0.0	16.80863872949102	0.0
23	0.0	0.0	0.0	17.20597761063228	0.0
24	0.0	0.0	0.0	17.474070856480953	0.0
25	1.5241230576956783E-4	0.0	0.0	17.71503471190264	0.0
26	1.5241230576956783E-4	0.0	0.0	17.93740426602044	0.0
27	1.5241230576956783E-4	0.0	0.0	18.185074262895988	0.0
28	1.5241230576956783E-4	0.0	0.0	18.40973000160033	0.0
29	1.5241230576956783E-4	0.0	0.0	18.643835303262385	0.0
30	1.5241230576956783E-4	0.0	0.0	19.03081014761132	0.0
31	1.5241230576956783E-4	0.0	0.0	19.26613474771953	0.0
32	1.5241230576956783E-4	0.0	0.0	19.50694619083545	0.0
33	1.5241230576956783E-4	0.0	0.0	19.79149996570723	0.0
34	1.5241230576956783E-4	0.0	0.0	20.0876370758175	0.0
35	1.5241230576956783E-4	0.0	0.0	20.404959496429743	0.0
36	1.5241230576956783E-4	0.0	0.0	20.63479725353025	0.0
37	1.5241230576956783E-4	0.0	0.0	20.88536308421542	0.0
38	1.5241230576956783E-4	0.0	0.0	21.144616416329455	0.0
39	1.5241230576956783E-4	0.0	0.0	21.462091249247464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGG	75	0.0	45.000004	2
TACGAGA	35	1.2108649E-7	45.000004	19
ATCCCAT	35	1.2108649E-7	45.000004	17
CGTACAG	70	0.0	45.000004	1
ACGTCCT	35	1.2108649E-7	45.000004	10
CACGTGC	35	1.2108649E-7	45.000004	17
CAATACG	35	1.2108649E-7	45.000004	1
TCAACGG	75	0.0	45.000004	2
TGATCGG	25	3.888969E-5	45.0	2
TCTGCGC	25	3.888969E-5	45.0	12
CTTCGTT	20	7.031071E-4	45.0	21
AACCGTA	20	7.031071E-4	45.0	32
AACGTAG	20	7.031071E-4	45.0	30
AGACTCG	25	3.888969E-5	45.0	14
TCGCAAT	20	7.031071E-4	45.0	11
CGAGTTT	20	7.031071E-4	45.0	7
TACCAAC	20	7.031071E-4	45.0	17
CCGATAG	20	7.031071E-4	45.0	1
ACGCAAC	25	3.888969E-5	45.0	45
ACCGCAA	25	3.888969E-5	45.0	31
>>END_MODULE