FastQCFastQC Report
Sat 18 Jun 2016
SRR3548745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398317
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC46341.1633949843968496No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT44921.127744987032941Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGT43841.1006309045308134No Hit
AATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT38910.9768601390349897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31220.783797829367062No Hit
AAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT12730.31959469467785706No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT8670.21766582897541406No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT8200.20586618196059922No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.17473519834704518No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT6870.1724756914718679No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6660.16720350876312084No Hit
AACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG6660.16720350876312084No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5850.14686794688652505No Hit
ATACTGGGAACAATAACTCAAAAATAATAACTCACCAAACAAAAACTAAAC5680.14259998945563457No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5650.14184682049724215No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTAT4910.12326865285689538No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC4700.11799647014814833No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT4410.11071583688368812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATG351.209246E-745.00000413
CTCACCG551.8189894E-1245.0000041
CCTACCG302.1619162E-645.0000041
CGCGACA302.1619162E-645.00000422
TCTAGCG302.1619162E-645.0000041
CGTTCAC600.045.00000421
CGTTAGG351.209246E-745.0000042
TAGCACG351.209246E-745.0000041
CCCAATC302.1619162E-645.00000434
TGACCGG351.209246E-745.0000042
CGCTCAG302.1619162E-645.0000041
CTTATCT302.1619162E-645.00000419
TATCTAT302.1619162E-645.00000421
TACGATG351.209246E-745.0000041
CGAGGTG351.209246E-745.00000414
CGCTAAG302.1619162E-645.0000041
TGCGAAG351.209246E-745.0000041
GATATCG351.209246E-745.0000049
CAAGCGT600.045.00000417
CCGACGG302.1619162E-645.0000042