##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398317
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13770941235247	31.0	31.0	34.0	28.0	34.0
2	31.358950283317057	31.0	31.0	34.0	28.0	34.0
3	31.307822663858182	31.0	31.0	34.0	28.0	34.0
4	35.32634810967144	37.0	35.0	37.0	33.0	37.0
5	34.98241350482154	35.0	35.0	37.0	32.0	37.0
6	35.01906521690011	36.0	35.0	37.0	32.0	37.0
7	34.93563418081578	36.0	35.0	37.0	32.0	37.0
8	35.28494390146542	37.0	35.0	37.0	33.0	37.0
9	35.76852356289086	39.0	35.0	39.0	32.0	39.0
10	35.67104592573252	38.0	35.0	39.0	30.0	39.0
11	36.10003339049049	38.0	35.0	39.0	31.0	39.0
12	35.74323968095763	37.0	35.0	39.0	31.0	39.0
13	35.422987218722774	37.0	35.0	39.0	30.0	39.0
14	36.36468189909042	38.0	35.0	40.0	31.0	41.0
15	36.54150839657861	38.0	35.0	40.0	31.0	41.0
16	36.58047986904902	38.0	35.0	40.0	31.0	41.0
17	36.53648475962613	38.0	35.0	40.0	31.0	41.0
18	36.35804648056698	38.0	35.0	40.0	31.0	41.0
19	36.351898111303306	38.0	35.0	40.0	31.0	41.0
20	36.36165918100407	38.0	35.0	40.0	31.0	41.0
21	36.36870131076505	38.0	35.0	40.0	31.0	41.0
22	36.33914947140092	38.0	35.0	40.0	31.0	41.0
23	36.31429238521078	38.0	35.0	40.0	31.0	41.0
24	35.9286447728819	38.0	34.0	40.0	30.0	41.0
25	35.99617641225456	38.0	34.0	40.0	30.0	41.0
26	35.91610199916147	38.0	34.0	40.0	30.0	41.0
27	35.74861228619416	38.0	34.0	40.0	30.0	41.0
28	35.617402721952615	37.0	34.0	40.0	29.0	41.0
29	35.52590273576072	37.0	34.0	40.0	29.0	41.0
30	35.41450151512489	37.0	34.0	40.0	29.0	41.0
31	35.39006620355144	37.0	34.0	40.0	29.0	41.0
32	35.24307021794199	37.0	34.0	40.0	28.0	41.0
33	35.14262007396119	37.0	34.0	40.0	27.0	41.0
34	35.19868095009753	37.0	34.0	40.0	28.0	41.0
35	34.82448150593623	37.0	34.0	40.0	26.0	41.0
36	34.899627683478236	37.0	34.0	40.0	27.0	41.0
37	34.63477832982273	37.0	33.0	40.0	25.0	41.0
38	34.70471508873585	37.0	33.0	40.0	26.0	41.0
39	34.69714825126721	37.0	33.0	40.0	26.0	41.0
40	34.40564926930058	36.0	33.0	40.0	25.0	41.0
41	34.50565755415912	36.0	33.0	40.0	25.0	41.0
42	34.337801298965395	36.0	33.0	40.0	24.0	41.0
43	34.34913147066281	36.0	33.0	40.0	25.0	41.0
44	34.332996081010855	36.0	33.0	40.0	24.0	41.0
45	34.160643407135524	36.0	33.0	39.0	24.0	41.0
46	33.994258341973854	36.0	33.0	39.0	23.0	41.0
47	33.81957084432751	35.0	33.0	39.0	23.0	40.0
48	33.741776022615156	35.0	33.0	39.0	23.0	40.0
49	33.570387405006564	35.0	33.0	39.0	23.0	40.0
50	33.46483077548787	35.0	32.0	39.0	23.0	40.0
51	31.568391507266824	34.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	3.0
12	5.0
13	22.0
14	11.0
15	29.0
16	78.0
17	167.0
18	335.0
19	604.0
20	1096.0
21	1634.0
22	2202.0
23	2808.0
24	3396.0
25	3933.0
26	4616.0
27	5694.0
28	7283.0
29	9309.0
30	11782.0
31	15640.0
32	20253.0
33	27949.0
34	44405.0
35	46952.0
36	40735.0
37	51054.0
38	58269.0
39	38041.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.42853807394613	36.83849798025191	20.372969268195934	24.35999467760603
2	20.959687886783644	35.924652977402424	20.843699867191205	22.27195926862273
3	20.18392385963943	31.51334238809792	24.55983550789949	23.742898244363158
4	22.15572019271083	32.20073459079075	19.169154216365357	26.474391000133057
5	17.04848148585172	38.37596688065034	18.69867467368955	25.876876959808392
6	21.163294561869066	35.576688918625116	22.709550433448737	20.550466086057085
7	75.25438281569704	10.911911869189614	7.777222664360297	6.056482650753043
8	77.7054456626255	6.579432964196859	5.958821742481491	9.756299630696153
9	70.82198349555756	12.330129017842573	11.014342847530987	5.833544639068882
10	40.96787232279817	30.644436466432516	12.578172661473147	15.809518549296165
11	33.9382953778021	23.40221481885031	25.112661523359534	17.54682827998805
12	32.42944689782259	22.292294830499326	29.48957739689745	15.788680874780638
13	24.499079878589164	34.35881471290455	22.60937896198254	18.532726446523746
14	15.053336915070158	35.77753397419643	31.41517936718744	17.75394974354597
15	13.429504640776067	28.375640507435033	36.52668603147744	21.668168820311458
16	15.51553159920365	25.031068219533687	36.36902266285396	23.084377518408704
17	15.774370664571183	25.01851540356048	29.073074962906432	30.134038968961907
18	19.90098338760334	26.051863214474903	27.941564131081524	26.10558926684023
19	22.221245892090973	31.171654737307218	27.37869586284291	19.228403507758895
20	24.804113306738103	29.146634464509425	24.519164384146293	21.53008784460618
21	19.75436649703628	31.257264942244493	24.78603725173668	24.202331308982544
22	17.343723717541558	30.23847839785899	25.65243261020745	26.765365274392007
23	20.54042383327852	29.845324201578137	21.8170452177537	27.79720674738964
24	17.26464097691035	32.094788823976884	27.685235628908632	22.955334570204133
25	17.595282149644632	28.187097211517457	27.54087824521675	26.67674239362116
26	21.78139522038979	32.77590461868311	22.421839891342827	23.020860269584276
27	16.282006542527686	32.11060537210312	26.664691690286883	24.942696395082308
28	19.20580843900712	29.422545359600523	30.933904402774676	20.437741798617683
29	22.28275469035969	28.858421809764582	27.26597157540351	21.592851924472217
30	22.46100467717923	31.932606441603046	24.27237602211304	21.334012859104686
31	28.90737779206009	29.37660205313858	20.89290690580618	20.82311324899515
32	28.976920392551662	27.550920497995314	21.270997722919184	22.20116138653384
33	28.81674646073354	28.688205625167896	22.394223696201767	20.1008242178968
34	24.579668957137155	27.157515245394997	23.622391211020368	24.64042458644748
35	23.678376770260872	28.352794382363793	26.966461386282788	21.002367461092547
36	29.92114321005631	24.76469746458223	28.332207764167737	16.98195156119372
37	25.20178651676931	30.664520971989646	23.7916031703392	20.342089340901843
38	25.647913596457094	28.593557392729913	27.191658904842125	18.566870105970875
39	26.97072934371367	24.959015055847477	26.773148020295395	21.297107580143454
40	28.3191528355556	25.382296010464028	23.417529254337627	22.881021899642747
41	22.54159375572722	21.288069552642742	30.564851613162382	25.605485078467655
42	25.793777318065764	24.696661202007448	24.878425977299486	24.631135502627306
43	23.35426306183266	23.94650491944858	27.589583171192793	25.109648847525968
44	21.813781485600664	24.86662633028467	26.3508712909567	26.96872089315796
45	25.472425229151657	27.9935327892106	25.571341419020527	20.96270056261721
46	21.930020561512563	26.119648420730222	31.731761386031724	20.218569631725487
47	23.948513370004292	25.14680518280666	25.57912416492392	25.32555728226513
48	23.735366554779233	20.428703771116975	34.051772834199895	21.784156839903897
49	23.060778224379074	20.149529143872847	31.49752583997168	25.2921667917764
50	20.474898133898378	24.155132720923284	26.63255648139547	28.73741266378287
51	20.465106937439277	23.738128174293337	30.807874130403672	24.98889075786371
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3628.0
1	2864.0
2	2100.0
3	1421.5
4	743.0
5	693.5
6	644.0
7	665.5
8	687.0
9	764.5
10	842.0
11	918.5
12	995.0
13	974.5
14	954.0
15	961.0
16	968.0
17	995.5
18	1023.0
19	1002.5
20	982.0
21	976.5
22	971.0
23	1318.0
24	1665.0
25	1786.5
26	2357.5
27	2807.0
28	2806.0
29	2805.0
30	3320.5
31	3836.0
32	4731.5
33	5627.0
34	5988.5
35	6350.0
36	7205.0
37	8060.0
38	9202.0
39	10344.0
40	13086.0
41	15828.0
42	21206.0
43	26584.0
44	37360.0
45	48136.0
46	47613.5
47	47091.0
48	46166.0
49	45241.0
50	41157.0
51	37073.0
52	32102.5
53	27132.0
54	23464.5
55	19797.0
56	18090.0
57	16383.0
58	14694.5
59	13006.0
60	12788.0
61	12570.0
62	11372.5
63	10175.0
64	8441.0
65	6707.0
66	5779.0
67	4851.0
68	4177.5
69	3504.0
70	2999.0
71	2494.0
72	2049.5
73	1605.0
74	1204.5
75	669.5
76	535.0
77	470.5
78	406.0
79	340.0
80	274.0
81	188.0
82	102.0
83	71.0
84	40.0
85	29.5
86	19.0
87	14.5
88	10.0
89	8.0
90	6.0
91	3.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.33496653083268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2900026864658	24.84549580410039
2	9.459425547276467	5.928215658496234
3	3.271515089421951	3.0753844749645274
4	1.4996615333286563	1.8796737581772267
5	0.922059134231481	1.444634605529601
6	0.580413647053746	1.0912345322680599
7	0.37300642423964525	0.8181700673534403
8	0.29935403379161324	0.7504198903783965
9	0.2489257264434813	0.7020071376092728
>10	2.8398258188421717	22.01772619472706
>50	1.0181111102877776	22.47469351138143
>100	0.18569322137324246	7.970854084200571
>500	0.007203616346375784	1.534930524379582
>1k	0.004802410897583856	5.466559756434211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	4634	1.1633949843968496	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	4492	1.127744987032941	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGT	4384	1.1006309045308134	No Hit
AATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	3891	0.9768601390349897	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3122	0.783797829367062	No Hit
AAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	1273	0.31959469467785706	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	867	0.21766582897541406	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTT	820	0.20586618196059922	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	696	0.17473519834704518	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	687	0.1724756914718679	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	666	0.16720350876312084	No Hit
AACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	666	0.16720350876312084	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	585	0.14686794688652505	No Hit
ATACTGGGAACAATAACTCAAAAATAATAACTCACCAAACAAAAACTAAAC	568	0.14259998945563457	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	565	0.14184682049724215	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTAT	491	0.12326865285689538	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	470	0.11799647014814833	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	441	0.11071583688368812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.510563194641454E-4	0.0	0.0	1.4980530582425555	0.0
2	2.510563194641454E-4	0.0	0.0	1.8618336651461023	0.0
3	2.510563194641454E-4	0.0	0.0	2.443782213663991	0.0
4	2.510563194641454E-4	0.0	0.0	5.522485859252806	0.0
5	2.510563194641454E-4	0.0	0.0	5.9204101256034765	0.0
6	2.510563194641454E-4	0.0	0.0	7.81136632380742	0.0
7	2.510563194641454E-4	0.0	0.0	9.258706005518217	0.0
8	2.510563194641454E-4	0.0	0.0	10.291802760113177	0.0
9	2.510563194641454E-4	0.0	0.0	12.800859616837846	0.0
10	2.510563194641454E-4	0.0	0.0	14.811569679426185	0.0
11	2.510563194641454E-4	0.0	0.0	17.977640924188524	0.0
12	2.510563194641454E-4	0.0	0.0	19.059442604759525	0.0
13	2.510563194641454E-4	0.0	0.0	19.565572144799244	0.0
14	2.510563194641454E-4	0.0	0.0	20.139235834774816	0.0
15	2.510563194641454E-4	0.0	0.0	20.51079918758175	0.0
16	2.510563194641454E-4	0.0	0.0	21.187395968537622	0.0
17	2.510563194641454E-4	0.0	0.0	22.01563076644984	0.0
18	2.510563194641454E-4	0.0	0.0	23.409495452114772	0.0
19	2.510563194641454E-4	0.0	0.0	23.831019012495073	0.0
20	2.510563194641454E-4	0.0	0.0	24.336897496215325	0.0
21	2.510563194641454E-4	0.0	0.0	24.798339011390425	0.0
22	2.510563194641454E-4	0.0	0.0	25.260031582884988	0.0
23	2.510563194641454E-4	0.0	0.0	25.74708084264543	0.0
24	2.510563194641454E-4	0.0	0.0	26.056131171905793	0.0
25	2.510563194641454E-4	0.0	0.0	26.33932270026135	0.0
26	2.510563194641454E-4	0.0	0.0	26.589876907086566	0.0
27	2.510563194641454E-4	0.0	0.0	26.931313501557806	0.0
28	2.510563194641454E-4	0.0	0.0	27.197182143870336	0.0
29	2.510563194641454E-4	0.0	0.0	27.478365221670177	0.0
30	2.510563194641454E-4	0.0	0.0	27.983992649070967	0.0
31	2.510563194641454E-4	0.0	0.0	28.250363404022423	0.0
32	2.510563194641454E-4	0.0	0.0	28.528282749669234	0.0
33	2.510563194641454E-4	0.0	0.0	28.79465350462069	0.0
34	2.510563194641454E-4	0.0	0.0	29.041943979292874	0.0
35	2.510563194641454E-4	0.0	0.0	29.35300275910895	0.0
36	2.510563194641454E-4	0.0	0.0	29.595021051072386	0.0
37	2.510563194641454E-4	0.0	0.0	29.826997090257258	0.0
38	2.510563194641454E-4	0.0	0.0	30.090355169375147	0.0
39	2.510563194641454E-4	0.0	0.0	30.342164657797685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATG	35	1.209246E-7	45.000004	13
CTCACCG	55	1.8189894E-12	45.000004	1
CCTACCG	30	2.1619162E-6	45.000004	1
CGCGACA	30	2.1619162E-6	45.000004	22
TCTAGCG	30	2.1619162E-6	45.000004	1
CGTTCAC	60	0.0	45.000004	21
CGTTAGG	35	1.209246E-7	45.000004	2
TAGCACG	35	1.209246E-7	45.000004	1
CCCAATC	30	2.1619162E-6	45.000004	34
TGACCGG	35	1.209246E-7	45.000004	2
CGCTCAG	30	2.1619162E-6	45.000004	1
CTTATCT	30	2.1619162E-6	45.000004	19
TATCTAT	30	2.1619162E-6	45.000004	21
TACGATG	35	1.209246E-7	45.000004	1
CGAGGTG	35	1.209246E-7	45.000004	14
CGCTAAG	30	2.1619162E-6	45.000004	1
TGCGAAG	35	1.209246E-7	45.000004	1
GATATCG	35	1.209246E-7	45.000004	9
CAAGCGT	60	0.0	45.000004	17
CCGACGG	30	2.1619162E-6	45.000004	2
>>END_MODULE