FastQCFastQC Report
Sat 18 Jun 2016
SRR3548743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417907
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC98662.360812333844611No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGT82701.9789091831436183No Hit
AATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT80191.9188479733529231TruSeq Adapter, Index 8 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT38010.909532503643155RNA PCR Primer, Index 46 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24210.5793154936385367No Hit
AATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT17840.4268892361219123No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT15220.36419586175871665RNA PCR Primer, Index 46 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG11380.2723093894096055RNA PCR Primer, Index 46 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTAT10090.24144127760482595No Hit
AAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT8500.20339453514777212RNA PCR Primer, Index 40 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5890.14094044847298562No Hit
AATGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC5740.13735113314684846No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.13567611932798446No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT5580.13352253013230217No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCGCATATCGTATGCC5290.126583187168437No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4510.1079187474725238No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.10026154144343119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.0281274E-445.0000041
TTTAGCG207.0281274E-445.00000413
CTTCGAT207.0281274E-445.00000418
TTCTACG207.0281274E-445.00000422
ACACGAA207.0281274E-445.00000430
CCCTCTA207.0281274E-445.00000432
CGTATCG351.209446E-745.00000426
CGAGTGT207.0281274E-445.00000420
GTACAAG406.7975634E-945.0000041
CGAAACG302.1621727E-645.0000041
CCTAGCG207.0281274E-445.0000041
CTATATG302.1621727E-645.0000041
TAGCGTG207.0281274E-445.00000415
ACATTTC302.1621727E-645.00000413
CGACCGT207.0281274E-445.00000422
ATTGTGC207.0281274E-445.00000436
CGTCTCA207.0281274E-445.00000423
AAGCGTA207.0281274E-445.00000436
CACGACG351.209446E-745.0000041
CACGAAA207.0281274E-445.00000431
Produced by FastQC (version 0.11.5)