##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417907
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03849421043438	31.0	31.0	34.0	28.0	34.0
2	31.252417403872155	31.0	31.0	34.0	28.0	34.0
3	31.192025977071452	31.0	31.0	34.0	28.0	34.0
4	35.181794035515075	37.0	35.0	37.0	32.0	37.0
5	34.78906072403669	35.0	35.0	37.0	32.0	37.0
6	34.86259861643858	35.0	35.0	37.0	32.0	37.0
7	34.74206462203313	35.0	35.0	37.0	32.0	37.0
8	35.157771944475684	37.0	35.0	37.0	32.0	37.0
9	35.67833273910224	39.0	35.0	39.0	32.0	39.0
10	35.5666858894443	37.0	35.0	39.0	30.0	39.0
11	35.9507545937254	37.0	35.0	39.0	31.0	39.0
12	35.44091867329334	37.0	35.0	39.0	30.0	39.0
13	35.23928529553226	37.0	34.0	39.0	30.0	39.0
14	36.104757757108636	38.0	34.0	40.0	30.0	41.0
15	36.294802432120065	38.0	35.0	40.0	31.0	41.0
16	36.24662185605889	38.0	35.0	40.0	31.0	41.0
17	36.23017800611141	38.0	35.0	40.0	30.0	41.0
18	35.97323327917455	38.0	34.0	40.0	30.0	41.0
19	35.988207902715196	38.0	34.0	40.0	30.0	41.0
20	36.06261201654914	38.0	34.0	40.0	30.0	41.0
21	36.15780065899829	38.0	34.0	40.0	30.0	41.0
22	36.14373772155049	38.0	34.0	40.0	30.0	41.0
23	36.09282687296456	38.0	34.0	40.0	30.0	41.0
24	35.701869076134166	37.0	34.0	40.0	29.0	41.0
25	35.82259450069034	37.0	34.0	40.0	30.0	41.0
26	35.72429511829187	37.0	34.0	40.0	30.0	41.0
27	35.64882138849074	37.0	34.0	40.0	29.0	41.0
28	35.521699804023385	37.0	34.0	40.0	29.0	41.0
29	35.25847377526579	37.0	34.0	40.0	28.0	41.0
30	35.366325522185555	37.0	34.0	40.0	29.0	41.0
31	35.12749008750751	37.0	34.0	40.0	27.0	41.0
32	34.853610970861936	37.0	34.0	40.0	26.0	41.0
33	34.83727958612801	37.0	33.0	40.0	26.0	41.0
34	34.76246150459313	37.0	33.0	40.0	26.0	41.0
35	34.75741732012146	37.0	33.0	40.0	26.0	41.0
36	34.573675482822736	37.0	33.0	40.0	25.0	41.0
37	34.27009837116871	36.0	33.0	40.0	24.0	41.0
38	34.05282275721632	36.0	33.0	40.0	23.0	41.0
39	33.67513346270821	36.0	32.0	39.0	23.0	41.0
40	33.59019829770738	35.0	32.0	39.0	22.0	41.0
41	33.73904959715917	36.0	33.0	39.0	23.0	41.0
42	33.86128253415234	36.0	33.0	39.0	23.0	41.0
43	33.823434400476664	36.0	33.0	39.0	23.0	41.0
44	33.78595476984114	35.0	33.0	39.0	23.0	41.0
45	33.41555896407574	35.0	32.0	39.0	23.0	40.0
46	33.07524401362026	35.0	31.0	39.0	22.0	40.0
47	33.03660623057283	35.0	31.0	39.0	21.0	40.0
48	32.99029449135813	35.0	31.0	39.0	21.0	40.0
49	32.91354535817778	35.0	31.0	39.0	20.0	40.0
50	32.77122661261956	35.0	31.0	39.0	20.0	40.0
51	30.734632346431145	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	7.0
11	12.0
12	9.0
13	13.0
14	27.0
15	55.0
16	107.0
17	168.0
18	347.0
19	631.0
20	1062.0
21	1655.0
22	2414.0
23	3241.0
24	4042.0
25	5130.0
26	6181.0
27	7580.0
28	9588.0
29	11850.0
30	14848.0
31	18352.0
32	23603.0
33	31130.0
34	47412.0
35	47748.0
36	41040.0
37	49425.0
38	55060.0
39	35161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.069809790216485	39.35827827722436	19.20283699483378	23.36907493772538
2	20.461729523554283	39.798567623897185	19.11669342700649	20.623009425542048
3	19.77999889927663	28.656854276190636	28.84278081008454	22.72036601444819
4	26.232630704917604	29.211044562546213	17.92815147867827	26.628173253857916
5	16.054050303057856	40.97035943403676	19.00111747350487	23.974472789400515
6	21.383704986994715	32.644344315840605	26.200326866982365	19.771623830182314
7	71.4620717049487	13.677205694089833	8.60646028901167	6.2542623119497875
8	74.14544384276884	5.303093750523442	6.330595084552304	14.220867322155408
9	70.3524946937955	10.90338281005104	11.37573670697069	7.36838578918276
10	43.66186735326281	25.997410907211417	14.24862469401087	16.09209704551491
11	36.272902822876866	22.884517368696862	22.38440609992175	18.458173708504525
12	33.1858523547105	21.50693814652542	30.297410667923725	15.009798830840355
13	26.254405884562832	32.216497928965055	20.798885876522768	20.730210309949342
14	16.2069551359513	33.05496198915069	33.745306970211075	16.992775904686926
15	14.795157774337353	27.440554956006956	33.522769419990574	24.24151784966512
16	15.940627938751922	24.549481104647686	37.525573871698725	21.984317084901665
17	17.099737501405816	24.834712029231383	28.43431672597013	29.63123374339267
18	20.310021129102886	26.45588611820333	27.419019064050136	25.815073688643647
19	22.59832929335953	29.29120593816328	29.43717142809285	18.673293340384344
20	26.312313505157846	31.03322030978184	22.48203547679268	20.17243070826763
21	20.246609891674463	28.849480865359993	25.150332490243045	25.753576752722495
22	18.738140303943222	31.689586439088124	24.692575142316354	24.879698114652303
23	23.705992003005452	28.664032906842912	19.44547471088065	28.18450037927099
24	18.20189659421833	33.670649211427424	25.705719215040666	22.42173497931358
25	18.257889913306073	24.063009234111895	28.139514293850066	29.53958655873197
26	22.154689919048977	30.530237588745823	21.959431165307112	25.355641326898088
27	17.10069465215945	28.075624481044827	27.636052997437226	27.187627869358494
28	21.32364377720402	25.204411507823508	26.2718738858167	27.20007082915577
29	18.493348998700668	30.341918177967823	26.41233575891287	24.752397064418638
30	23.524372647502915	26.068957926045748	21.568195794758164	28.838473631693173
31	29.86621425341045	27.887305070266834	17.71590329905936	24.530577377263363
32	24.36953676296401	28.315390744830786	19.455046218417017	27.860026273788186
33	27.338618400744664	27.80690440696136	19.926921539959846	24.92755565233413
34	18.251429145719023	26.546576152110397	25.132864488989178	30.0691302131814
35	19.199008391819234	29.171083518581888	22.621061623758397	29.008846465840488
36	24.938323598312543	25.502563967581303	26.313031368223072	23.24608106588308
37	19.927400115336667	31.54433881222377	21.022380577496907	27.50588049494265
38	26.130694149655305	31.868334342329753	21.555752834960888	20.445218673054054
39	20.41817916426382	29.501300528586505	27.646103080350414	22.434417226799262
40	21.9120522030021	32.05904662999184	21.985992098720526	24.042909068285528
41	17.419904428497247	23.46407215002381	32.9269430758518	26.189080345627136
42	24.00079443512552	27.054823202291416	20.03914746582374	28.905234896759325
43	22.123821807244195	23.823721545702753	26.117294039104394	27.935162607948662
44	20.62803446699864	23.080493985503953	25.40278100151469	30.888690545982715
45	26.519297355631753	28.01580255894254	22.367895249421522	23.097004836004185
46	18.205725197232876	25.74615883438181	33.169341504210266	22.878774464175045
47	22.57535767527225	27.493198247456966	22.968507347328472	26.962936729942307
48	22.023560265800764	19.827377861581645	33.912329776720654	24.236732095896933
49	22.987650362401205	20.56031605117885	29.317049008511464	27.134984577908483
50	20.130794650484436	23.992897941408017	25.119225090749858	30.757082317357693
51	18.478991737396118	23.091501219170773	30.944923152758868	27.48458389067424
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2892.0
1	2223.0
2	1554.0
3	1057.0
4	560.0
5	537.0
6	514.0
7	539.0
8	564.0
9	581.0
10	598.0
11	626.0
12	654.0
13	746.5
14	839.0
15	857.0
16	875.0
17	965.5
18	1056.0
19	1039.0
20	1022.0
21	984.5
22	947.0
23	966.0
24	985.0
25	1267.5
26	2192.0
27	2834.0
28	2821.0
29	2808.0
30	3296.5
31	3785.0
32	4199.5
33	4614.0
34	5686.0
35	6758.0
36	7137.5
37	7517.0
38	8529.5
39	9542.0
40	12302.5
41	15063.0
42	19827.5
43	24592.0
44	39178.0
45	53764.0
46	52838.0
47	51912.0
48	46154.0
49	40396.0
50	37012.5
51	33629.0
52	31347.5
53	29066.0
54	26163.0
55	23260.0
56	21414.5
57	19569.0
58	18024.0
59	16479.0
60	16700.0
61	16921.0
62	15233.0
63	13545.0
64	11211.0
65	8877.0
66	7712.5
67	6548.0
68	5324.0
69	4100.0
70	3326.0
71	2552.0
72	2207.5
73	1863.0
74	1573.0
75	1179.0
76	1075.0
77	723.0
78	371.0
79	336.5
80	302.0
81	221.5
82	141.0
83	89.0
84	37.0
85	36.5
86	36.0
87	30.0
88	24.0
89	21.0
90	18.0
91	12.5
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.323420442935113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59379194088542	24.438794381139044
2	8.377914009501346	5.080940178897312
3	2.847993010494388	2.590826684272855
4	1.3515295202007516	1.6393199152834301
5	0.7938094574535562	1.2035508964971202
6	0.4907018138487708	0.8927854448068295
7	0.37090188673509344	0.7872909698182295
8	0.2874137630610991	0.6972294702710273
9	0.24173919184228382	0.659732323659203
>10	3.5158320789348494	25.465356356057306
>50	0.9874250887028764	20.648114584437394
>100	0.1291369334507749	5.987704905799719
>500	0.004724521955516155	0.8755797698914372
>1k	0.004724521955516155	2.7876721607533486
>5k	0.0023622609777580773	6.245101958415746
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC	9866	2.360812333844611	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGT	8270	1.9789091831436183	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT	8019	1.9188479733529231	TruSeq Adapter, Index 8 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT	3801	0.909532503643155	RNA PCR Primer, Index 46 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2421	0.5793154936385367	No Hit
AATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT	1784	0.4268892361219123	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT	1522	0.36419586175871665	RNA PCR Primer, Index 46 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTG	1138	0.2723093894096055	RNA PCR Primer, Index 46 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTAT	1009	0.24144127760482595	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT	850	0.20339453514777212	RNA PCR Primer, Index 40 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	589	0.14094044847298562	No Hit
AATGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC	574	0.13735113314684846	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.13567611932798446	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTT	558	0.13352253013230217	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCGCATATCGTATGCC	529	0.126583187168437	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	451	0.1079187474725238	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	419	0.10026154144343119	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.785753768182873E-4	0.0	0.0	1.331157410620066	0.0
2	4.785753768182873E-4	0.0	0.0	1.5737951266669379	0.0
3	4.785753768182873E-4	0.0	0.0	2.25217572330686	0.0
4	4.785753768182873E-4	0.0	0.0	6.23129069386251	0.0
5	4.785753768182873E-4	0.0	0.0	6.658658505361241	0.0
6	4.785753768182873E-4	0.0	0.0	8.356643942312525	0.0
7	4.785753768182873E-4	0.0	0.0	9.798830840354434	0.0
8	7.17863065227431E-4	0.0	0.0	10.596137418133699	0.0
9	7.17863065227431E-4	0.0	0.0	14.269203435214054	0.0
10	7.17863065227431E-4	0.0	0.0	15.817873354598033	0.0
11	7.17863065227431E-4	0.0	0.0	20.301646060008565	0.0
12	7.17863065227431E-4	0.0	0.0	21.13628151717966	0.0
13	7.17863065227431E-4	0.0	0.0	21.60289250957749	0.0
14	7.17863065227431E-4	0.0	0.0	22.25088356978945	0.0
15	7.17863065227431E-4	0.0	0.0	22.581339867482477	0.0
16	7.17863065227431E-4	0.0	0.0	23.124283632482825	0.0
17	7.17863065227431E-4	0.0	0.0	23.679909644968856	0.0
18	7.17863065227431E-4	0.0	0.0	24.691378703874307	0.0
19	7.17863065227431E-4	0.0	0.0	25.01680996011074	0.0
20	7.17863065227431E-4	0.0	0.0	25.377177218854914	0.0
21	7.17863065227431E-4	0.0	0.0	25.74639812207022	0.0
22	7.17863065227431E-4	0.0	0.0	26.091450968756206	0.0
23	7.17863065227431E-4	0.0	0.0	26.464021899609243	0.0
24	7.17863065227431E-4	0.0	0.0	26.692541642039977	0.0
25	7.17863065227431E-4	0.0	0.0	26.90359338321684	0.0
26	7.17863065227431E-4	0.0	0.0	27.126370221125754	0.0
27	7.17863065227431E-4	0.0	0.0	27.36182930652035	0.0
28	7.17863065227431E-4	0.0	0.0	27.568334581617442	0.0
29	7.17863065227431E-4	0.0	0.0	27.779864898171123	0.0
30	7.17863065227431E-4	0.0	0.0	28.15243582902416	0.0
31	7.17863065227431E-4	0.0	0.0	28.391723517433306	0.0
32	7.17863065227431E-4	0.0	0.0	28.62790046589313	0.0
33	7.17863065227431E-4	0.0	0.0	28.862880975910908	0.0
34	7.17863065227431E-4	0.0	0.0	29.07752203241391	0.0
35	7.17863065227431E-4	0.0	0.0	29.333320571323284	0.0
36	7.17863065227431E-4	0.0	0.0	29.571411821290383	0.0
37	7.17863065227431E-4	0.0	0.0	29.787010028547023	0.0
38	7.17863065227431E-4	0.0	0.0	30.02773344308662	0.0
39	7.17863065227431E-4	0.0	0.0	30.281617680488722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0281274E-4	45.000004	1
TTTAGCG	20	7.0281274E-4	45.000004	13
CTTCGAT	20	7.0281274E-4	45.000004	18
TTCTACG	20	7.0281274E-4	45.000004	22
ACACGAA	20	7.0281274E-4	45.000004	30
CCCTCTA	20	7.0281274E-4	45.000004	32
CGTATCG	35	1.209446E-7	45.000004	26
CGAGTGT	20	7.0281274E-4	45.000004	20
GTACAAG	40	6.7975634E-9	45.000004	1
CGAAACG	30	2.1621727E-6	45.000004	1
CCTAGCG	20	7.0281274E-4	45.000004	1
CTATATG	30	2.1621727E-6	45.000004	1
TAGCGTG	20	7.0281274E-4	45.000004	15
ACATTTC	30	2.1621727E-6	45.000004	13
CGACCGT	20	7.0281274E-4	45.000004	22
ATTGTGC	20	7.0281274E-4	45.000004	36
CGTCTCA	20	7.0281274E-4	45.000004	23
AAGCGTA	20	7.0281274E-4	45.000004	36
CACGACG	35	1.209446E-7	45.000004	1
CACGAAA	20	7.0281274E-4	45.000004	31
>>END_MODULE