FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR3548742_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 729963 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 51 |
| %GC | 48 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC | 12135 | 1.6624130264136676 | No Hit |
| AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT | 12098 | 1.6573442763537332 | No Hit |
| AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT | 11195 | 1.5336393762423575 | No Hit |
| CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT | 7753 | 1.0621086274235818 | TruSeq Adapter, Index 16 (95% over 23bp) |
| AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3985 | 0.5459180807794368 | No Hit |
| AATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT | 2637 | 0.36125118670398365 | No Hit |
| AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT | 2261 | 0.3097417266354596 | No Hit |
| AATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT | 2227 | 0.30508395631011437 | No Hit |
| AACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG | 1867 | 0.2557663881593999 | No Hit |
| AAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT | 1636 | 0.22412094859602474 | No Hit |
| AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTAT | 1374 | 0.18822871844189362 | No Hit |
| ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 977 | 0.1338424002312446 | No Hit |
| CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 967 | 0.13247246778261362 | No Hit |
| AATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC | 894 | 0.12247196090760765 | No Hit |
| AAAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT | 883 | 0.1209650352141136 | No Hit |
| TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 846 | 0.11589628515417906 | No Hit |
| CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC | 816 | 0.11178648780828618 | TruSeq Adapter, Index 19 (95% over 21bp) |
| GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 762 | 0.104388852585679 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TCCGAGT | 35 | 1.2111195E-7 | 45.000004 | 16 |
| ATTGGCC | 35 | 1.2111195E-7 | 45.000004 | 41 |
| CGGTCTA | 35 | 1.2111195E-7 | 45.000004 | 31 |
| GTTATGG | 35 | 1.2111195E-7 | 45.000004 | 2 |
| CTTGTAC | 25 | 3.8894017E-5 | 45.0 | 19 |
| TTGATCG | 20 | 7.031594E-4 | 45.0 | 21 |
| GTCATGC | 20 | 7.031594E-4 | 45.0 | 20 |
| TTAGGTA | 20 | 7.031594E-4 | 45.0 | 43 |
| GTGCAAA | 20 | 7.031594E-4 | 45.0 | 40 |
| TCGTTCA | 20 | 7.031594E-4 | 45.0 | 16 |
| ATCTCGC | 25 | 3.8894017E-5 | 45.0 | 10 |
| ATCTCGA | 20 | 7.031594E-4 | 45.0 | 27 |
| CGAACCG | 45 | 3.8380676E-10 | 45.0 | 1 |
| ATTAGCG | 25 | 3.8894017E-5 | 45.0 | 1 |
| CTCGTAG | 25 | 3.8894017E-5 | 45.0 | 1 |
| GTTTCGG | 20 | 7.031594E-4 | 45.0 | 28 |
| TTAGCTA | 25 | 3.8894017E-5 | 45.0 | 22 |
| TCACATC | 20 | 7.031594E-4 | 45.0 | 16 |
| CCGATAG | 25 | 3.8894017E-5 | 45.0 | 1 |
| TTTCGGC | 20 | 7.031594E-4 | 45.0 | 15 |