##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729963
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34305300405637	31.0	31.0	34.0	30.0	34.0
2	31.508272337091057	31.0	31.0	34.0	30.0	34.0
3	31.69840252177165	31.0	31.0	34.0	30.0	34.0
4	35.514558957097826	37.0	35.0	37.0	33.0	37.0
5	35.10106128666796	37.0	35.0	37.0	32.0	37.0
6	35.11305367532327	37.0	35.0	37.0	32.0	37.0
7	35.06966928460758	37.0	35.0	37.0	32.0	37.0
8	35.39512687629373	37.0	35.0	37.0	33.0	37.0
9	36.26591073794151	39.0	37.0	39.0	32.0	39.0
10	35.96932036281291	38.0	35.0	39.0	30.0	39.0
11	36.25100587290041	38.0	35.0	39.0	32.0	39.0
12	35.81807023095691	37.0	35.0	39.0	31.0	39.0
13	35.548903163585	37.0	35.0	39.0	30.0	39.0
14	36.51027106853361	38.0	35.0	40.0	31.0	41.0
15	36.666358431865724	38.0	35.0	40.0	31.0	41.0
16	36.67637126813277	38.0	35.0	40.0	31.0	41.0
17	36.62881680304344	38.0	35.0	40.0	31.0	41.0
18	36.42124189856198	38.0	35.0	40.0	31.0	41.0
19	36.43260822808827	38.0	35.0	40.0	31.0	41.0
20	36.43266165545377	38.0	35.0	40.0	31.0	41.0
21	36.51756869868747	38.0	35.0	40.0	31.0	41.0
22	36.44907481612082	38.0	35.0	40.0	31.0	41.0
23	36.405297254792366	38.0	35.0	40.0	31.0	41.0
24	36.096204876137556	38.0	34.0	40.0	30.0	41.0
25	36.155555829542045	38.0	35.0	40.0	30.0	41.0
26	36.049117558013215	38.0	35.0	40.0	30.0	41.0
27	36.026639706396075	38.0	34.0	40.0	30.0	41.0
28	35.98543350827371	38.0	35.0	40.0	30.0	41.0
29	35.74884480446269	38.0	34.0	40.0	29.0	41.0
30	35.79953778479183	38.0	34.0	40.0	30.0	41.0
31	35.630362086845494	38.0	34.0	40.0	29.0	41.0
32	35.63559933859661	38.0	34.0	40.0	29.0	41.0
33	35.635495223730516	38.0	34.0	40.0	29.0	41.0
34	35.55678301502953	38.0	34.0	40.0	29.0	41.0
35	35.49972532854405	38.0	34.0	40.0	29.0	41.0
36	35.38174263627061	38.0	34.0	40.0	28.0	41.0
37	35.220996132680696	38.0	34.0	40.0	27.0	41.0
38	35.16450285836405	37.0	34.0	40.0	27.0	41.0
39	35.04715581474677	37.0	34.0	40.0	27.0	41.0
40	34.83380664499433	37.0	34.0	40.0	26.0	41.0
41	34.91504363919815	37.0	34.0	40.0	26.0	41.0
42	34.798066477342005	37.0	34.0	40.0	26.0	41.0
43	34.72391614369496	37.0	33.0	40.0	26.0	41.0
44	34.54575505881805	37.0	33.0	40.0	25.0	41.0
45	34.175297926059265	36.0	33.0	40.0	24.0	41.0
46	34.17878988387083	36.0	33.0	40.0	24.0	41.0
47	34.031402961519966	36.0	33.0	39.0	23.0	41.0
48	34.012166370076294	36.0	33.0	39.0	23.0	41.0
49	33.87682937354359	36.0	33.0	39.0	23.0	41.0
50	33.55200606058115	35.0	33.0	39.0	23.0	40.0
51	31.489470014233596	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	6.0
11	9.0
12	10.0
13	9.0
14	26.0
15	64.0
16	114.0
17	275.0
18	547.0
19	1017.0
20	1735.0
21	2674.0
22	3694.0
23	4744.0
24	5799.0
25	6724.0
26	7990.0
27	9890.0
28	12173.0
29	15653.0
30	20285.0
31	26437.0
32	34909.0
33	47693.0
34	76739.0
35	83491.0
36	72352.0
37	94338.0
38	117844.0
39	82712.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.347231298024695	38.12522004539956	18.252157986089706	25.275390670486036
2	19.919091789583856	38.15864639714616	19.769221179703628	22.15304063356636
3	19.954984019737985	30.461132961533664	25.19593458846544	24.387948430262902
4	23.92847308699208	30.465516745369285	18.77465022199755	26.831359945641083
5	16.77523381322067	37.934662441795	18.609710355182386	26.68039338980195
6	20.99311882931053	33.782945162974016	23.99751768240308	21.22641832531238
7	74.94873027810999	11.15398999675326	8.087259217247999	5.810020507888756
8	77.01732827554272	5.682890776655803	6.196478451647549	11.103302496153916
9	71.92652230318522	10.301207047480489	11.36509658708729	6.407174062246991
10	41.327026164339834	28.924205747414593	13.802754386181219	15.946013702064352
11	33.639650228847216	23.705996057334414	24.314109071281695	18.34024464253668
12	31.213225875832062	21.6565771141825	31.07554766474465	16.054649345240787
13	24.79125654313986	33.57649634296533	22.601693510493	19.030553603401817
14	15.869571471430744	34.279134695868144	32.16519193438572	17.686101898315393
15	13.693433776780466	28.400617565547844	34.776968147700636	23.128980509971054
16	14.965553048579174	24.562614817463352	36.54664688484211	23.925185249115366
17	15.634628056490534	25.095792526470518	29.067089701806804	30.20248971523214
18	20.03046729765755	26.10200790998996	27.215215017747475	26.652309774605015
19	22.44730212353229	30.00247957773202	27.885111985128013	19.665106313607676
20	24.2372558609135	29.817264710677115	24.980444214295794	20.96503521411359
21	20.47596932995234	29.177369263921598	25.564857396881756	24.781804009244304
22	18.169825045926984	30.38030694706444	25.99104338165085	25.458824625357725
23	21.622739782701313	28.37253395035091	22.121943166982437	27.88278309996534
24	16.598923507081867	32.11244953511343	27.105346435367274	24.18328052243744
25	18.65491812598721	25.291144893645296	28.887765544281013	27.16617143608649
26	22.044131003900198	31.071985840378215	22.645805335338913	24.238077820382678
27	16.568511006722257	29.016539194452324	27.871960633621157	26.54298916520426
28	19.618665603599087	26.124885781882096	32.7424814682388	21.513967146280017
29	21.682057857727035	28.203484286189852	27.148636300744013	22.965821555339105
30	21.070930992392764	25.722125641984594	25.68267158746402	27.52427177815862
31	26.476821427935388	27.362893735709893	24.940716173285495	21.219568663069225
32	25.406219219330296	23.698598422111804	22.08714688278721	28.808035475770687
33	23.754765652505675	23.887375113533153	26.761767377250628	25.596091856710544
34	20.21486020524328	23.18322983493684	28.768855407739846	27.83305455208004
35	20.276507165431674	21.827681677016507	30.884168101670905	27.011643055880914
36	25.41909658434743	21.458621875355327	32.032719466603105	21.089562073694147
37	19.21754938263994	25.535814828970782	26.547509942284748	28.69912584610453
38	21.990429651913864	24.77468036051142	28.25088394891248	24.984006038662233
39	19.535784690456914	22.932121217102786	29.584924167389303	27.947169925050996
40	21.48259569320637	23.56283811645248	26.985066366377474	27.969499823963677
41	18.0669431190348	19.279333336073197	35.73989366584334	26.913829879048663
42	24.1273872785333	22.143725092915666	27.578110123389816	26.150777505161223
43	22.052487591836847	21.88453387363469	28.437879728150605	27.625098806377856
44	20.852700753325852	22.266060060578415	26.823003357704433	30.0582358283913
45	25.390054016436448	26.463122103449077	25.403479354433035	22.74334452568144
46	19.322897187939663	23.7001053478053	33.1901753924514	23.78682207180364
47	21.99632036144298	24.54138086450957	26.070773450161173	27.391525323886277
48	21.39683792192207	19.752781990320056	35.60413336018401	23.246246727573862
49	21.801926946982245	19.425642121586982	31.83133939665435	26.94109153477642
50	20.067181487280862	22.434013778780567	27.16822633475943	30.330578399179135
51	18.812734344069494	22.5912820238834	32.382874200473175	26.213109431573926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4681.0
1	3714.0
2	2747.0
3	1943.0
4	1139.0
5	1066.5
6	994.0
7	1050.0
8	1106.0
9	1218.0
10	1330.0
11	1419.5
12	1509.0
13	1607.5
14	1706.0
15	1761.0
16	1816.0
17	1866.0
18	1916.0
19	1892.0
20	1868.0
21	2058.5
22	2249.0
23	2121.5
24	1994.0
25	2568.5
26	3692.5
27	4242.0
28	5507.5
29	6773.0
30	6584.0
31	6395.0
32	7524.0
33	8653.0
34	9815.0
35	10977.0
36	13281.5
37	15586.0
38	16922.0
39	18258.0
40	22545.5
41	26833.0
42	36050.0
43	45267.0
44	68935.5
45	92604.0
46	89253.0
47	85902.0
48	82077.5
49	78253.0
50	72137.5
51	66022.0
52	58276.0
53	50530.0
54	46733.0
55	42936.0
56	35802.0
57	28668.0
58	27297.5
59	25927.0
60	24530.0
61	23133.0
62	21910.5
63	20688.0
64	17721.0
65	14754.0
66	11474.0
67	8194.0
68	7508.0
69	6822.0
70	5855.0
71	4888.0
72	4200.0
73	3512.0
74	2863.5
75	1892.0
76	1569.0
77	1221.0
78	873.0
79	774.0
80	675.0
81	477.5
82	280.0
83	249.0
84	218.0
85	136.5
86	55.0
87	42.0
88	29.0
89	22.5
90	16.0
91	15.0
92	14.0
93	9.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	729963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.35728096405524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62423475325076	19.683395302579605
2	9.826163004169148	4.983295521906443
3	3.8112138624285046	2.8992606217110533
4	1.89803913093467	1.9251644609552663
5	1.088545946289648	1.3801282701174997
6	0.6898368675941451	1.0495432362569104
7	0.4692805045958614	0.8329774324193611
8	0.33743069270764525	0.6845059904706837
9	0.25934746397898295	0.5918711860287241
>10	2.321877620725538	13.699393143904
>50	0.9661391416291671	17.75332702998325
>100	0.6954907883379591	25.175109086072307
>500	0.006469681751981014	1.2530956072449269
>1k	0.0037739810219889255	2.185603259112442
>5k	5.391401459984179E-4	1.0599225663288148
>10k+	0.0016174204379952534	4.8434072849087135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	12135	1.6624130264136676	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	12098	1.6573442763537332	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT	11195	1.5336393762423575	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	7753	1.0621086274235818	TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3985	0.5459180807794368	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	2637	0.36125118670398365	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2261	0.3097417266354596	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2227	0.30508395631011437	No Hit
AACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1867	0.2557663881593999	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	1636	0.22412094859602474	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTAT	1374	0.18822871844189362	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	977	0.1338424002312446	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	967	0.13247246778261362	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	894	0.12247196090760765	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	883	0.1209650352141136	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	846	0.11589628515417906	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	816	0.11178648780828618	TruSeq Adapter, Index 19 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.104388852585679	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4245927533313332	0.0
2	0.0	0.0	0.0	1.7528285680233107	0.0
3	0.0	0.0	0.0	2.4431375288884505	0.0
4	0.0	0.0	0.0	6.1490787889249185	0.0
5	0.0	0.0	0.0	6.591840956322444	0.0
6	0.0	0.0	0.0	8.533309222522238	0.0
7	0.0	0.0	0.0	10.130513464381071	0.0
8	0.0	0.0	0.0	11.087685266239522	0.0
9	0.0	0.0	0.0	13.912485975316557	0.0
10	1.369932448630958E-4	0.0	0.0	15.775183125720071	0.0
11	1.369932448630958E-4	0.0	0.0	19.305362052597186	0.0
12	1.369932448630958E-4	0.0	0.0	20.351990443351237	0.0
13	1.369932448630958E-4	0.0	0.0	20.8662630845673	0.0
14	1.369932448630958E-4	0.0	0.0	21.473280152555677	0.0
15	1.369932448630958E-4	0.0	0.0	21.81850312961068	0.0
16	1.369932448630958E-4	0.0	0.0	22.451685907367906	0.0
17	1.369932448630958E-4	0.0	0.0	23.141035915519005	0.0
18	1.369932448630958E-4	0.0	0.0	24.37616701120468	0.0
19	1.369932448630958E-4	0.0	0.0	24.739884076316198	0.0
20	1.369932448630958E-4	0.0	0.0	25.18333120993804	0.0
21	1.369932448630958E-4	0.0	0.0	25.56746026853416	0.0
22	1.369932448630958E-4	0.0	0.0	25.978850982858035	0.0
23	1.369932448630958E-4	0.0	0.0	26.402708082464454	0.0
24	1.369932448630958E-4	0.0	0.0	26.67217379511016	0.0
25	1.369932448630958E-4	0.0	0.0	26.8958837639716	0.0
26	1.369932448630958E-4	0.0	0.0	27.12247059097516	0.0
27	1.369932448630958E-4	0.0	0.0	27.419334952593488	0.0
28	2.739864897261916E-4	0.0	0.0	27.655648299982328	0.0
29	2.739864897261916E-4	0.0	0.0	27.89689340418624	0.0
30	2.739864897261916E-4	0.0	0.0	28.345409287868016	0.0
31	2.739864897261916E-4	0.0	0.0	28.583914527174667	0.0
32	2.739864897261916E-4	0.0	0.0	28.827762503030975	0.0
33	2.739864897261916E-4	0.0	0.0	29.06503480313386	0.0
34	2.739864897261916E-4	0.0	0.0	29.299978218074067	0.0
35	2.739864897261916E-4	0.0	0.0	29.59355474181568	0.0
36	2.739864897261916E-4	0.0	0.0	29.816579744452802	0.0
37	2.739864897261916E-4	0.0	0.0	30.04056369980396	0.0
38	2.739864897261916E-4	0.0	0.0	30.26742451329725	0.0
39	2.739864897261916E-4	0.0	0.0	30.51935509060048	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAGT	35	1.2111195E-7	45.000004	16
ATTGGCC	35	1.2111195E-7	45.000004	41
CGGTCTA	35	1.2111195E-7	45.000004	31
GTTATGG	35	1.2111195E-7	45.000004	2
CTTGTAC	25	3.8894017E-5	45.0	19
TTGATCG	20	7.031594E-4	45.0	21
GTCATGC	20	7.031594E-4	45.0	20
TTAGGTA	20	7.031594E-4	45.0	43
GTGCAAA	20	7.031594E-4	45.0	40
TCGTTCA	20	7.031594E-4	45.0	16
ATCTCGC	25	3.8894017E-5	45.0	10
ATCTCGA	20	7.031594E-4	45.0	27
CGAACCG	45	3.8380676E-10	45.0	1
ATTAGCG	25	3.8894017E-5	45.0	1
CTCGTAG	25	3.8894017E-5	45.0	1
GTTTCGG	20	7.031594E-4	45.0	28
TTAGCTA	25	3.8894017E-5	45.0	22
TCACATC	20	7.031594E-4	45.0	16
CCGATAG	25	3.8894017E-5	45.0	1
TTTCGGC	20	7.031594E-4	45.0	15
>>END_MODULE