FastQCFastQC Report
Sat 18 Jun 2016
SRR3548741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740737
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC112351.5167326594999304No Hit
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT109501.4782574652001992No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT105381.422637184317781No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT72050.9726799120335557TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43130.582257940402599No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT25160.3396617152916622No Hit
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT22000.2970014998575743No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT21540.29079146849691595No Hit
AACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG17750.23962621011236104No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTAT14060.18981095854534064No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT14030.18940595650008032No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA9950.13432567834467563No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9890.13351567425415498No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC8750.11812559653426251No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8350.11272556926412479No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8330.11245556790061789No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT8070.1089455501750284No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC7750.10462552835891821TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.0316595E-445.00000429
GTCATAG207.0316595E-445.0000041
TACCCGT207.0316595E-445.00000435
CGAACCG800.045.0000041
GCGACTA207.0316595E-445.00000440
TACTTCG207.0316595E-445.0000041
CGAAACG207.0316595E-445.0000041
TCCGATT207.0316595E-445.00000444
ACCATAC207.0316595E-445.00000413
TCCGAGT207.0316595E-445.00000416
TAGACAA207.0316595E-445.00000415
CCGGCAT207.0316595E-445.00000418
CGTTGTG207.0316595E-445.00000429
CGGCATG800.045.0000041
CCGTGCC207.0316595E-445.00000417
GTTAGTA207.0316595E-445.00000435
CCAGCGA207.0316595E-445.00000411
TCTAGTA406.8102963E-945.00000431
TAACGAA453.8380676E-1045.00000415
GCGTTCC207.0316595E-445.0000049
Produced by FastQC (version 0.11.5)