##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740737
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146494639797933	31.0	31.0	34.0	28.0	34.0
2	31.35400418772115	31.0	31.0	34.0	28.0	34.0
3	31.326867700681888	31.0	31.0	34.0	28.0	34.0
4	35.3259064958278	37.0	35.0	37.0	33.0	37.0
5	34.98105940434999	35.0	35.0	37.0	32.0	37.0
6	35.042598115120484	36.0	35.0	37.0	32.0	37.0
7	34.940494399496714	36.0	35.0	37.0	32.0	37.0
8	35.31867045928582	37.0	35.0	37.0	33.0	37.0
9	35.8165516235857	39.0	37.0	39.0	32.0	39.0
10	35.66475145699486	37.0	35.0	39.0	30.0	39.0
11	36.084976179129704	37.0	35.0	39.0	31.0	39.0
12	35.717454373144584	37.0	35.0	39.0	31.0	39.0
13	35.43014997225736	37.0	35.0	39.0	30.0	39.0
14	36.38685390361221	38.0	35.0	40.0	31.0	41.0
15	36.56707306371897	38.0	35.0	40.0	31.0	41.0
16	36.589124075076576	38.0	35.0	40.0	31.0	41.0
17	36.56488335266093	38.0	35.0	40.0	31.0	41.0
18	36.379257415249945	38.0	35.0	40.0	31.0	41.0
19	36.37030282002924	38.0	35.0	40.0	31.0	41.0
20	36.35646795016315	38.0	35.0	40.0	31.0	41.0
21	36.417079206249994	38.0	35.0	40.0	31.0	41.0
22	36.38125677534671	38.0	35.0	40.0	31.0	41.0
23	36.31825060716557	38.0	35.0	40.0	31.0	41.0
24	35.98482592337091	38.0	34.0	40.0	30.0	41.0
25	36.090126455138595	38.0	34.0	40.0	30.0	41.0
26	35.989345746196015	38.0	34.0	40.0	30.0	41.0
27	35.93452736936322	38.0	34.0	40.0	30.0	41.0
28	35.87846428624464	38.0	34.0	40.0	30.0	41.0
29	35.63703581703088	38.0	34.0	40.0	29.0	41.0
30	35.72750112388068	38.0	34.0	40.0	29.0	41.0
31	35.61582180990014	38.0	34.0	40.0	29.0	41.0
32	35.60258229304058	38.0	34.0	40.0	29.0	41.0
33	35.54442804936165	38.0	34.0	40.0	29.0	41.0
34	35.5447088507797	38.0	34.0	40.0	29.0	41.0
35	35.44969402095481	38.0	34.0	40.0	29.0	41.0
36	35.37034197022695	38.0	34.0	40.0	28.0	41.0
37	35.213425277797654	37.0	34.0	40.0	27.0	41.0
38	35.178480351325774	37.0	34.0	40.0	27.0	41.0
39	35.01447207308397	37.0	34.0	40.0	27.0	41.0
40	34.766919972945864	37.0	33.0	40.0	26.0	41.0
41	34.843634110352255	37.0	34.0	40.0	26.0	41.0
42	34.79780137889696	37.0	33.0	40.0	26.0	41.0
43	34.757951877656986	37.0	33.0	40.0	26.0	41.0
44	34.67574861253049	37.0	33.0	40.0	26.0	41.0
45	34.36528214467483	36.0	33.0	40.0	24.0	41.0
46	34.25159807057025	36.0	33.0	40.0	24.0	41.0
47	34.081926513728895	36.0	33.0	39.0	24.0	41.0
48	34.00092880469047	36.0	33.0	39.0	23.0	41.0
49	33.88570032278663	36.0	33.0	39.0	24.0	40.0
50	33.70674746907472	35.0	33.0	39.0	23.0	40.0
51	31.779578986873883	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	11.0
11	11.0
12	8.0
13	17.0
14	20.0
15	56.0
16	110.0
17	287.0
18	591.0
19	1064.0
20	1865.0
21	2690.0
22	3726.0
23	4829.0
24	5713.0
25	6685.0
26	8269.0
27	10010.0
28	12757.0
29	16279.0
30	21035.0
31	27684.0
32	36268.0
33	50141.0
34	79630.0
35	84498.0
36	75299.0
37	96565.0
38	117020.0
39	77588.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.604983955168976	37.62509500672979	18.385337845956123	25.384583192145122
2	20.15371177624447	37.61672496446107	19.895185470686627	22.33437778860783
3	20.321247622300493	30.562264339434915	24.64855947522535	24.467928563039244
4	23.84476541606535	30.63651471439931	18.735259613061046	26.783460256474296
5	16.956760631641192	37.84973614116751	18.405047945492125	26.788455281699175
6	20.85234030431854	34.04798194230881	23.673854552965494	21.425823200407162
7	75.78060769206884	11.048995797428777	7.542893091610113	5.627503418892266
8	77.94048359944217	5.761964097918694	5.856734576509611	10.440817726129517
9	72.06322891930604	11.272016923685465	10.51938812291002	6.145366034098472
10	41.907451632630746	29.17243232078322	13.493318141256614	15.426797905329423
11	34.132492369086464	23.981386105999835	23.65144443979442	18.23467708511928
12	31.786315520893382	21.812195151585513	30.384333440883875	16.017155886637227
13	24.856055523080393	33.74504041245408	22.542278838508135	18.85662522595739
14	16.119351402724583	34.6514349897467	31.38954851722001	17.839665090308706
15	13.94138540399629	28.249432659634934	35.06858709636484	22.74059484000394
16	15.246976997233835	24.56121403413087	36.63419000265951	23.55761896597578
17	16.010405852549557	24.798410231971673	28.864765767067123	30.326418148411648
18	20.40467804362412	26.130191957469385	27.345063092568616	26.120066906337875
19	22.901110650608786	29.7069000198451	27.46710370887373	19.924885620672384
20	24.608734274108084	29.65478975668827	24.838640435134195	20.897835534069447
21	20.80306505547853	29.18201736918771	25.631904441117427	24.383013134216327
22	18.618214021980812	30.123242122372716	25.704399807219026	25.554144048427446
23	21.380597972019757	28.78619537028662	22.31223767680027	27.52096898089335
24	16.985515776854673	31.74865033068417	27.402168380950325	23.863665511510835
25	18.77697482372286	25.49393374436541	28.926056076583183	26.803035355328543
26	21.825560219079108	31.26278287705353	22.78717007520888	24.124486828658483
27	16.74345955447075	29.503318991760906	27.858605685958715	25.894615767809626
28	19.46520829930191	26.643599550177726	32.55446940007047	21.33672275044989
29	21.46888841788651	28.47677380770773	27.318062956217933	22.736274818187834
30	21.127876695777314	26.1582720992741	25.97345616595364	26.740395038994947
31	26.663579651077242	27.65232464423945	24.590374181389617	21.09372152329369
32	25.683474701547244	24.12151681365991	22.25985741227993	27.935151072512916
33	23.954250968967393	24.305117740844594	26.518453918192286	25.22217737199573
34	20.390907974085266	23.706119715904567	28.349198163450723	27.55377414655944
35	20.280477416410953	22.402283131529817	30.5970945153273	26.72014493673193
36	25.709529833125654	21.921950705851064	31.457993862869003	20.91052559815427
37	19.3608527723065	25.935655975062673	26.55841411999131	28.145077132639518
38	22.044801326246695	25.306417797409875	27.753305154191025	24.895475722152398
39	19.625319107861493	23.53142883371561	29.20847750281139	27.634774555611507
40	21.74563981548107	23.8466554256099	26.817345427594407	27.590359331314623
41	18.212266971948207	19.777869878242885	35.03902194706083	26.970841202748076
42	23.913345762396098	22.5684689707683	27.248402604433153	26.269782662402445
43	22.118781699847585	22.254187383646286	28.122937020831955	27.504093895674174
44	20.81494511547283	22.783120054756274	26.829765490315726	29.57216933945516
45	25.00266626346463	27.08397177405746	25.262407575158253	22.650954387319654
46	19.589003924469818	24.268127554044145	32.53475930053447	23.608109220951565
47	22.034001271706423	24.9897061980163	26.071061658861378	26.905230871415903
48	21.50641860741397	20.36863286159595	34.90402126530739	23.220927265682693
49	21.795050065002826	19.975510876329924	31.594614552803492	26.63482450586375
50	20.176256890097292	23.089841603700098	27.107866894727817	29.62603461147479
51	19.03104610678284	23.254542435439298	31.794820563843846	25.919590893934014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5026.0
1	3996.5
2	2967.0
3	2075.5
4	1184.0
5	1115.0
6	1046.0
7	1120.0
8	1194.0
9	1301.0
10	1408.0
11	1396.5
12	1385.0
13	1530.5
14	1676.0
15	1718.0
16	1760.0
17	1842.0
18	1924.0
19	1920.5
20	1917.0
21	2063.5
22	2210.0
23	2205.0
24	2200.0
25	2653.5
26	3800.5
27	4494.0
28	5801.0
29	7108.0
30	6967.0
31	6826.0
32	7874.0
33	8922.0
34	10271.5
35	11621.0
36	14117.5
37	16614.0
38	17879.5
39	19145.0
40	23563.5
41	27982.0
42	37115.0
43	46248.0
44	69065.5
45	91883.0
46	88450.0
47	85017.0
48	81709.5
49	78402.0
50	72268.0
51	66134.0
52	58454.5
53	50775.0
54	47256.0
55	43737.0
56	36726.0
57	29715.0
58	28070.0
59	26425.0
60	25257.0
61	24089.0
62	22615.5
63	21142.0
64	18087.5
65	15033.0
66	11822.0
67	8611.0
68	7873.5
69	7136.0
70	6040.5
71	4945.0
72	4251.5
73	3558.0
74	2934.5
75	1949.5
76	1588.0
77	1269.5
78	951.0
79	806.5
80	662.0
81	495.0
82	328.0
83	270.0
84	212.0
85	129.5
86	47.0
87	39.0
88	31.0
89	25.0
90	19.0
91	18.5
92	18.0
93	10.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	740737.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.294612753363705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42501939139362	19.584358829269767
2	9.935508804388705	5.026296954292958
3	3.795384920452894	2.880083754384362
4	1.9558443907578615	1.9788930588023466
5	1.1127701754478652	1.4073545335723168
6	0.7130315345069683	1.0821513927774271
7	0.5215841197509861	0.9235287829183693
8	0.3632330988226189	0.7350272459137946
9	0.24539527505782124	0.5586460608683483
>10	2.237697152200769	13.151394392560109
>50	0.9616303126618708	17.557195310270277
>100	0.7205515798951573	26.3383055015743
>500	0.006443084171938814	1.2126721866207926
>1k	0.003758465766964308	2.141359879768175
>5k	5.369236809949011E-4	0.9785309782285598
>10k+	0.0016107710429847035	4.4442011381780935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	11235	1.5167326594999304	No Hit
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	10950	1.4782574652001992	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT	10538	1.422637184317781	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	7205	0.9726799120335557	TruSeq Adapter, Index 16 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4313	0.582257940402599	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	2516	0.3396617152916622	No Hit
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2200	0.2970014998575743	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2154	0.29079146849691595	No Hit
AACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1775	0.23962621011236104	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTAT	1406	0.18981095854534064	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	1403	0.18940595650008032	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	995	0.13432567834467563	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	989	0.13351567425415498	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	875	0.11812559653426251	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	835	0.11272556926412479	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	833	0.11245556790061789	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	807	0.1089455501750284	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	775	0.10462552835891821	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.050020452603286E-4	0.0	0.0	1.26144637030417	0.0
2	4.050020452603286E-4	0.0	0.0	1.5537228463003738	0.0
3	4.050020452603286E-4	0.0	0.0	2.168110948960292	0.0
4	4.050020452603286E-4	0.0	0.0	5.4634775905618325	0.0
5	4.050020452603286E-4	0.0	0.0	5.860109593553448	0.0
6	4.050020452603286E-4	0.0	0.0	7.62200349111763	0.0
7	4.050020452603286E-4	0.0	0.0	9.081360860872348	0.0
8	4.050020452603286E-4	0.0	0.0	9.971825357718057	0.0
9	4.050020452603286E-4	0.0	0.0	12.567888467836763	0.0
10	5.400027270137714E-4	0.0	0.0	14.293737183372777	0.0
11	5.400027270137714E-4	0.0	0.0	17.56642371043974	0.0
12	5.400027270137714E-4	0.0	0.0	18.5570587131465	0.0
13	5.400027270137714E-4	0.0	0.0	19.039551149733306	0.0
14	5.400027270137714E-4	0.0	0.0	19.63179914058566	0.0
15	5.400027270137714E-4	0.0	0.0	19.965385825198418	0.0
16	5.400027270137714E-4	0.0	0.0	20.56465385150195	0.0
17	5.400027270137714E-4	0.0	0.0	21.22588719073031	0.0
18	5.400027270137714E-4	0.0	0.0	22.39661310289617	0.0
19	5.400027270137714E-4	0.0	0.0	22.750179888408436	0.0
20	5.400027270137714E-4	0.0	0.0	23.164496980709753	0.0
21	5.400027270137714E-4	0.0	0.0	23.541013882120104	0.0
22	5.400027270137714E-4	0.0	0.0	23.932785860568597	0.0
23	5.400027270137714E-4	0.0	0.0	24.32077781992799	0.0
24	5.400027270137714E-4	0.0	0.0	24.56931407503608	0.0
25	5.400027270137714E-4	0.0	0.0	24.787205175386134	0.0
26	5.400027270137714E-4	0.0	0.0	24.993351216423644	0.0
27	5.400027270137714E-4	0.0	0.0	25.27671764742412	0.0
28	5.400027270137714E-4	0.0	0.0	25.516748859581742	0.0
29	5.400027270137714E-4	0.0	0.0	25.751650045832733	0.0
30	5.400027270137714E-4	0.0	0.0	26.187837248578106	0.0
31	5.400027270137714E-4	0.0	0.0	26.42557344914592	0.0
32	5.400027270137714E-4	0.0	0.0	26.66438965516776	0.0
33	5.400027270137714E-4	0.0	0.0	26.903880864598367	0.0
34	5.400027270137714E-4	0.0	0.0	27.134597029715	0.0
35	5.400027270137714E-4	0.0	0.0	27.416208451852683	0.0
36	5.400027270137714E-4	0.0	0.0	27.63922957810937	0.0
37	6.750034087672143E-4	0.0	0.0	27.86198070300255	0.0
38	6.750034087672143E-4	0.0	0.0	28.0874318415308	0.0
39	6.750034087672143E-4	0.0	0.0	28.319093011419707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.0316595E-4	45.000004	29
GTCATAG	20	7.0316595E-4	45.000004	1
TACCCGT	20	7.0316595E-4	45.000004	35
CGAACCG	80	0.0	45.000004	1
GCGACTA	20	7.0316595E-4	45.000004	40
TACTTCG	20	7.0316595E-4	45.000004	1
CGAAACG	20	7.0316595E-4	45.000004	1
TCCGATT	20	7.0316595E-4	45.000004	44
ACCATAC	20	7.0316595E-4	45.000004	13
TCCGAGT	20	7.0316595E-4	45.000004	16
TAGACAA	20	7.0316595E-4	45.000004	15
CCGGCAT	20	7.0316595E-4	45.000004	18
CGTTGTG	20	7.0316595E-4	45.000004	29
CGGCATG	80	0.0	45.000004	1
CCGTGCC	20	7.0316595E-4	45.000004	17
GTTAGTA	20	7.0316595E-4	45.000004	35
CCAGCGA	20	7.0316595E-4	45.000004	11
TCTAGTA	40	6.8102963E-9	45.000004	31
TAACGAA	45	3.8380676E-10	45.000004	15
GCGTTCC	20	7.0316595E-4	45.000004	9
>>END_MODULE