FastQCFastQC Report
Sat 18 Jun 2016
SRR3548738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965004
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC71220.7380280288993621No Hit
AATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT64260.6659039755275626No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT59130.6127435741199No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT47530.4925368185002342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34080.353159157889501No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG17280.17906661526791598No Hit
ATGATCATGCACATCAATATTTGGAAAATAATCAATTGACACAAATTTTAT16300.16891121694832353No Hit
AAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT15120.1566832883594265No Hit
AATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT13410.1389631545568723No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12310.12756423807569708No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA11920.12352280405055316No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT11590.1201031291062006No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT10230.10600992327492943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG351.2116652E-745.0000041
TACGGGT700.045.0000044
TAAGGTT351.2116652E-745.00000421
GAACCGT351.2116652E-745.00000410
TCATACG351.2116652E-745.0000041
CACGTCA351.2116652E-745.00000431
TCGCCGA207.0327247E-445.042
AACGTCG207.0327247E-445.01
AAACTCG453.8380676E-1045.01
ATCTCCG253.890339E-545.018
GTATTCG207.0327247E-445.022
CGTATAT207.0327247E-445.030
CGTTGTA207.0327247E-445.015
CCAATCG406.8139343E-945.01
CGCGCGA207.0327247E-445.014
ATTGTCG207.0327247E-445.018
ATTCGTT207.0327247E-445.027
ATTAACG406.8139343E-945.01
TAACGTT207.0327247E-445.010
CGATTGT207.0327247E-445.016