##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	965004
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623749746115042	31.0	31.0	34.0	30.0	34.0
2	31.80285470319294	31.0	31.0	34.0	30.0	34.0
3	31.97591512574041	33.0	31.0	34.0	30.0	34.0
4	35.77321130275108	37.0	35.0	37.0	35.0	37.0
5	35.39892788009169	37.0	35.0	37.0	33.0	37.0
6	35.41610293843342	37.0	35.0	37.0	33.0	37.0
7	35.39810508557478	37.0	35.0	37.0	33.0	37.0
8	35.696618874118656	37.0	35.0	37.0	35.0	37.0
9	36.54427339161288	39.0	37.0	39.0	33.0	39.0
10	36.27859262759532	38.0	35.0	39.0	32.0	39.0
11	36.53926304968684	39.0	35.0	39.0	32.0	39.0
12	36.18257333648358	37.0	35.0	39.0	32.0	39.0
13	35.97613585021409	38.0	35.0	39.0	31.0	39.0
14	37.03941019933596	39.0	35.0	40.0	32.0	41.0
15	37.057165566153095	39.0	35.0	40.0	32.0	41.0
16	37.09702343202723	39.0	35.0	40.0	32.0	41.0
17	37.022388508234165	38.0	35.0	40.0	32.0	41.0
18	36.89287194664478	38.0	35.0	40.0	32.0	41.0
19	36.92560652598331	39.0	35.0	40.0	32.0	41.0
20	36.93433602347762	39.0	35.0	40.0	32.0	41.0
21	36.95779188480048	39.0	35.0	40.0	32.0	41.0
22	36.94973181458315	39.0	35.0	40.0	32.0	41.0
23	36.87950930773344	39.0	35.0	40.0	32.0	41.0
24	36.55454485162756	38.0	35.0	40.0	31.0	41.0
25	36.64549576996572	38.0	35.0	40.0	31.0	41.0
26	36.562502331596555	38.0	35.0	40.0	31.0	41.0
27	36.48641145528931	38.0	35.0	40.0	31.0	41.0
28	36.40243149251195	38.0	35.0	40.0	31.0	41.0
29	36.22448507985459	38.0	35.0	40.0	30.0	41.0
30	36.19834736436326	38.0	35.0	40.0	30.0	41.0
31	36.01622273068298	38.0	35.0	40.0	30.0	41.0
32	35.98729228065376	38.0	35.0	40.0	30.0	41.0
33	35.97315037036116	38.0	35.0	40.0	30.0	41.0
34	35.741205217802204	38.0	34.0	40.0	29.0	41.0
35	35.78983092298063	38.0	34.0	40.0	30.0	41.0
36	35.586797567678474	38.0	34.0	40.0	29.0	41.0
37	35.555575935436536	38.0	34.0	40.0	29.0	41.0
38	35.44901782790538	38.0	34.0	40.0	29.0	41.0
39	35.3499083941621	38.0	34.0	40.0	28.0	41.0
40	35.195720432246915	37.0	34.0	40.0	27.0	41.0
41	35.15076932323596	37.0	34.0	40.0	27.0	41.0
42	35.13904191070711	37.0	34.0	40.0	27.0	41.0
43	35.044794632975616	37.0	34.0	40.0	27.0	41.0
44	34.898174515338795	37.0	34.0	40.0	27.0	41.0
45	34.64369577742683	36.0	33.0	40.0	26.0	41.0
46	34.53711487206271	36.0	33.0	40.0	26.0	41.0
47	34.41760034155299	36.0	33.0	40.0	25.0	41.0
48	34.371807785252706	36.0	33.0	40.0	25.0	41.0
49	34.228059158304006	36.0	33.0	39.0	24.0	41.0
50	33.97842703242681	35.0	33.0	39.0	24.0	41.0
51	32.08146598356069	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	12.0
11	13.0
12	12.0
13	20.0
14	31.0
15	91.0
16	174.0
17	307.0
18	611.0
19	1123.0
20	1935.0
21	2930.0
22	4017.0
23	5334.0
24	6308.0
25	7535.0
26	9012.0
27	10907.0
28	13935.0
29	17810.0
30	23050.0
31	30090.0
32	39888.0
33	57056.0
34	97236.0
35	104165.0
36	95587.0
37	131543.0
38	172900.0
39	131359.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.14758488047718	34.116542522103536	19.362924920518466	26.37294767690082
2	23.225810462961814	33.16887805646401	19.400748597933273	24.2045628826409
3	21.291517962619842	30.0509635193222	21.645298879590136	27.012219638467823
4	21.203124546634005	31.619765306672303	18.871113487612487	28.305996659081206
5	17.825418340234858	36.640262631035725	17.19941057239141	28.334908456338002
6	20.677530870338362	36.71829339567505	20.624474095444164	21.97970163854243
7	81.72163016940863	7.94742819718882	5.845260745033181	4.485680888369375
8	83.77892734123382	5.058735507832092	4.595006860075192	6.567330290858899
9	77.59957471678874	9.715711022959491	8.027220612557047	4.657493647694724
10	43.107075203833354	29.632312404922672	11.836634874052336	15.42397751719164
11	34.834052501336785	25.266838272172965	21.281466190813717	18.617643035676537
12	32.868879299982176	23.036277569833906	27.116260657986913	16.978582472197008
13	25.419065620453384	31.14940456205363	22.69317018375053	20.73835963374245
14	19.082304322054625	32.50059067112675	29.504333660793115	18.912771346025508
15	17.849666944385724	27.13346265922214	32.920070797634	22.09679959875814
16	20.21939805430858	24.513991651848073	33.05302361440989	22.213586679433455
17	19.031630956970126	24.58621933173334	28.03086826583102	28.35128144546551
18	24.080625572536487	25.134507214477868	27.236881919660437	23.547985293325212
19	23.58549809119962	29.921119497950265	24.94549245391729	21.54788995693282
20	29.109620271004054	26.6844489763773	22.865811955183606	21.340118797435036
21	22.649336168554743	29.64609473121355	23.686844821368616	24.017724278863092
22	22.81969815669158	26.080513655901942	24.826529216459207	26.27325897094727
23	23.900729945160847	28.830035937674868	21.293486866375684	25.9757472507886
24	20.88043158370328	28.869621265818584	27.419160956845772	22.830786193632356
25	20.486443579508478	25.726318232877794	26.367559098200626	27.4196790894131
26	23.142183866595374	29.189205433345357	24.272749128501022	23.395861571558253
27	20.926338129168375	26.71512242436301	26.39439836518812	25.964141081280488
28	20.134838819320954	28.61957048882699	29.288894139298904	21.95669655255315
29	22.72892133089604	27.27460197056178	28.175738131655415	21.820738566886767
30	23.224877824340627	25.173159904000396	27.974702695532866	23.627259576126107
31	28.811175912224197	26.51719578364442	21.18105209926591	23.49057620486547
32	28.59335298092029	24.76321341673195	24.910155812825646	21.733277789522116
33	25.71740635271978	24.388603570555148	24.971813588337458	24.922176488387613
34	21.08219240542008	27.538020567790394	26.39211858189189	24.987668444897636
35	23.595031730438425	25.232848775756374	29.2000862172592	21.972033276546004
36	25.489013517042412	26.35450215750401	26.987556528263095	21.168927797190477
37	24.656478107862764	27.381130026404037	23.707051991494335	24.255339874238864
38	24.599379898943425	28.108173644876082	22.34156542356301	24.95088103261748
39	24.62290311750003	25.60103377809833	24.13596213072692	25.640100973674723
40	26.049011195808514	24.4689141184907	22.48954408479136	26.992530600909426
41	20.765820659810736	24.585079440085224	27.569730280910754	27.07936961919329
42	26.41211849899068	24.293785310734464	23.160940265532577	26.13315592474228
43	23.47710475811499	24.18591011021716	25.470567997645606	26.866417134022242
44	21.918561995597948	24.829534385349696	26.20248206225052	27.04942155680184
45	26.21895867789149	26.012741916095685	24.05948576378958	23.708813642223244
46	22.115555997695346	25.25678650036684	29.316458791880656	23.31119871005716
47	23.282805045367688	26.066109570530276	25.424765078693973	25.226320305408063
48	24.558343799611194	22.423430369200542	29.573659798301357	23.44456603288691
49	23.903631487537876	22.50384454364956	28.71988095386133	24.872643014951233
50	21.16934230324434	25.211087207928674	26.108803694077952	27.510766794749035
51	21.76685277988485	24.72083017272467	28.21584159236646	25.29647545502402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4008.0
1	3249.0
2	2490.0
3	1788.5
4	1087.0
5	1005.0
6	923.0
7	1014.0
8	1105.0
9	1215.5
10	1326.0
11	1401.0
12	1476.0
13	1651.5
14	1827.0
15	2055.0
16	2283.0
17	2163.0
18	2043.0
19	1852.0
20	1661.0
21	1724.0
22	1787.0
23	3123.5
24	4460.0
25	4005.5
26	3992.5
27	4434.0
28	6364.0
29	8294.0
30	8282.0
31	8270.0
32	11159.5
33	14049.0
34	14456.5
35	14864.0
36	16987.5
37	19111.0
38	22801.5
39	26492.0
40	30777.0
41	35062.0
42	45933.0
43	56804.0
44	71291.5
45	85779.0
46	86968.0
47	88157.0
48	87620.5
49	87084.0
50	85343.5
51	83603.0
52	76883.5
53	70164.0
54	64803.0
55	59442.0
56	57277.0
57	55112.0
58	51546.5
59	47981.0
60	46163.5
61	44346.0
62	40864.5
63	37383.0
64	31913.0
65	26443.0
66	22918.0
67	19393.0
68	16563.0
69	13733.0
70	12060.5
71	10388.0
72	8865.5
73	7343.0
74	5782.5
75	3428.5
76	2635.0
77	2223.5
78	1812.0
79	1492.5
80	1173.0
81	968.0
82	763.0
83	508.0
84	253.0
85	205.0
86	157.0
87	124.0
88	91.0
89	77.5
90	64.0
91	55.5
92	47.0
93	33.0
94	19.0
95	12.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	965004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.750068627495917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94715740878732	18.51250337776926
2	9.595811153626693	4.558023468702495
3	3.5641215980902237	2.5394439765414973
4	1.7355554040630445	1.6487823981327487
5	0.9951844884969792	1.1817849949411339
6	0.6234142219271731	0.8883678332476398
7	0.4144869808050145	0.6890865967525873
8	0.3097509525037828	0.5885285103517657
9	0.2194536023628351	0.469083430500168
>10	2.6042319773927693	15.245698516418974
>50	1.1138820592043621	19.177508417405914
>100	0.8644346239851284	29.398215036850463
>500	0.00690511931291166	1.1631491307634452
>1k	0.004315699570569787	1.9451076008545911
>5k	0.001294709871170936	1.9947167107673076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	7122	0.7380280288993621	No Hit
AATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	6426	0.6659039755275626	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGT	5913	0.6127435741199	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	4753	0.4925368185002342	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3408	0.353159157889501	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1728	0.17906661526791598	No Hit
ATGATCATGCACATCAATATTTGGAAAATAATCAATTGACACAAATTTTAT	1630	0.16891121694832353	No Hit
AAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	1512	0.1566832883594265	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	1341	0.1389631545568723	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1231	0.12756423807569708	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	1192	0.12352280405055316	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	1159	0.1201031291062006	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	1023	0.10600992327492943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0725302693045831E-4	0.0	0.0	0.6700490360661717	0.0
2	2.0725302693045831E-4	0.0	0.0	0.8544006035208144	0.0
3	2.0725302693045831E-4	0.0	0.0	1.2031038213313106	0.0
4	3.1087954039568747E-4	0.0	0.0	3.0511790624702075	0.0
5	3.1087954039568747E-4	0.0	0.0	3.28983092298063	0.0
6	3.1087954039568747E-4	0.0	0.0	4.394593183033439	0.0
7	3.1087954039568747E-4	0.0	0.0	5.320703333872191	0.0
8	3.1087954039568747E-4	0.0	0.0	5.920597220322403	0.0
9	3.1087954039568747E-4	0.0	0.0	7.379140397345504	0.0
10	3.1087954039568747E-4	0.0	0.0	8.626596366439932	0.0
11	3.1087954039568747E-4	0.0	0.0	10.588971651930976	0.0
12	3.1087954039568747E-4	0.0	0.0	11.219124480313035	0.0
13	3.1087954039568747E-4	0.0	0.0	11.518397851200616	0.0
14	3.1087954039568747E-4	0.0	0.0	11.855805779043402	0.0
15	3.1087954039568747E-4	0.0	0.0	12.08305872307265	0.0
16	3.1087954039568747E-4	0.0	0.0	12.49227982474684	0.0
17	3.1087954039568747E-4	0.0	0.0	13.008236235290216	0.0
18	3.1087954039568747E-4	0.0	0.0	13.727818744792767	0.0
19	3.1087954039568747E-4	0.0	0.0	13.988957558725145	0.0
20	3.1087954039568747E-4	0.0	0.0	14.277142892671948	0.0
21	3.1087954039568747E-4	0.0	0.0	14.588436939121497	0.0
22	3.1087954039568747E-4	0.0	0.0	14.89527504549204	0.0
23	3.1087954039568747E-4	0.0	0.0	15.230817696092451	0.0
24	3.1087954039568747E-4	0.0	0.0	15.466153508171987	0.0
25	3.1087954039568747E-4	0.0	0.0	15.654546509651773	0.0
26	3.1087954039568747E-4	0.0	0.0	15.842006872510373	0.0
27	3.1087954039568747E-4	0.0	0.0	16.043560441200245	0.0
28	3.1087954039568747E-4	0.0	0.0	16.248533684834467	0.0
29	3.1087954039568747E-4	0.0	0.0	16.45350692846869	0.0
30	3.1087954039568747E-4	0.0	0.0	16.80801323103324	0.0
31	3.1087954039568747E-4	0.0	0.0	17.033297271306648	0.0
32	3.1087954039568747E-4	0.0	0.0	17.259928456255103	0.0
33	4.1450605386091663E-4	0.0	0.0	17.49049744871524	0.0
34	4.1450605386091663E-4	0.0	0.0	17.695159812809067	0.0
35	4.1450605386091663E-4	0.0	0.0	17.9427235534775	0.0
36	4.1450605386091663E-4	0.0	0.0	18.165831436968137	0.0
37	4.1450605386091663E-4	0.0	0.0	18.37235907830434	0.0
38	4.1450605386091663E-4	0.0	0.0	18.63671031415414	0.0
39	4.1450605386091663E-4	0.0	0.0	18.869248210370113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	35	1.2116652E-7	45.000004	1
TACGGGT	70	0.0	45.000004	4
TAAGGTT	35	1.2116652E-7	45.000004	21
GAACCGT	35	1.2116652E-7	45.000004	10
TCATACG	35	1.2116652E-7	45.000004	1
CACGTCA	35	1.2116652E-7	45.000004	31
TCGCCGA	20	7.0327247E-4	45.0	42
AACGTCG	20	7.0327247E-4	45.0	1
AAACTCG	45	3.8380676E-10	45.0	1
ATCTCCG	25	3.890339E-5	45.0	18
GTATTCG	20	7.0327247E-4	45.0	22
CGTATAT	20	7.0327247E-4	45.0	30
CGTTGTA	20	7.0327247E-4	45.0	15
CCAATCG	40	6.8139343E-9	45.0	1
CGCGCGA	20	7.0327247E-4	45.0	14
ATTGTCG	20	7.0327247E-4	45.0	18
ATTCGTT	20	7.0327247E-4	45.0	27
ATTAACG	40	6.8139343E-9	45.0	1
TAACGTT	20	7.0327247E-4	45.0	10
CGATTGT	20	7.0327247E-4	45.0	16
>>END_MODULE