SRR3548736_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638768
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	12131	1.8991245647872155	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	11404	1.7853117250707613	RNA PCR Primer, Index 27 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	10131	1.5860218420459384	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	5289	0.828000150289307	RNA PCR Primer, Index 38 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2962	0.4637051323798312	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	2455	0.38433359216491747	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	2123	0.3323585401898655	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	1827	0.2860193372241565	RNA PCR Primer, Index 38 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1461	0.2287215389625028	RNA PCR Primer, Index 27 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	1186	0.18566991458557724	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	933	0.1460624201588057	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	912	0.1427748415700223	No Hit
AATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	816	0.127745910878441	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.11146456929589459	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTCG	20	7.0309296E-4	45.000004	6
AACGTAA	20	7.0309296E-4	45.000004	12
CGAACAC	20	7.0309296E-4	45.000004	33
ATAGCGA	20	7.0309296E-4	45.000004	40
ATTAACG	35	1.2107921E-7	45.000004	1
ATAGACG	35	1.2107921E-7	45.000004	1
TAAGTAC	20	7.0309296E-4	45.000004	32
AGGGTAC	80	0.0	45.000004	6
GCGCATT	20	7.0309296E-4	45.000004	26
ACTTACG	20	7.0309296E-4	45.000004	28
CTCGGTA	20	7.0309296E-4	45.000004	38
GCTTATT	20	7.0309296E-4	45.000004	38
TACGAAC	20	7.0309296E-4	45.000004	31
TGCGAGA	35	1.2107921E-7	45.000004	12
ACTACAT	20	7.0309296E-4	45.000004	32
GTACTTG	35	1.2107921E-7	45.000004	35
AATCTAG	40	6.8066583E-9	45.000004	1
AGTCTAG	20	7.0309296E-4	45.000004	31
TACTCGT	20	7.0309296E-4	45.000004	43
TCATCGG	20	7.0309296E-4	45.000004	2