##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	638768
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35909438168474	31.0	31.0	34.0	30.0	34.0
2	31.538560165819202	31.0	31.0	34.0	30.0	34.0
3	31.709468852541143	31.0	31.0	34.0	30.0	34.0
4	35.50064186058162	37.0	35.0	37.0	33.0	37.0
5	35.085309533351705	37.0	35.0	37.0	32.0	37.0
6	35.09322946672344	37.0	35.0	37.0	32.0	37.0
7	35.03379004583824	37.0	35.0	37.0	32.0	37.0
8	35.38862153395286	37.0	35.0	37.0	33.0	37.0
9	36.28678487338126	39.0	37.0	39.0	32.0	39.0
10	35.99452696440648	38.0	35.0	39.0	30.0	39.0
11	36.23900383237733	38.0	35.0	39.0	32.0	39.0
12	35.84551198557222	37.0	35.0	39.0	31.0	39.0
13	35.669748641134184	37.0	35.0	39.0	30.0	39.0
14	36.65657640958846	38.0	35.0	40.0	31.0	41.0
15	36.7455570723643	38.0	35.0	40.0	31.0	41.0
16	36.720317861884126	38.0	35.0	40.0	31.0	41.0
17	36.68597675525386	38.0	35.0	40.0	31.0	41.0
18	36.46248246624753	38.0	35.0	40.0	31.0	41.0
19	36.47278980788017	38.0	35.0	40.0	31.0	41.0
20	36.542121709290385	38.0	35.0	40.0	31.0	41.0
21	36.65712590486687	38.0	35.0	40.0	31.0	41.0
22	36.60209653583148	38.0	35.0	40.0	31.0	41.0
23	36.547779475490316	38.0	35.0	40.0	31.0	41.0
24	36.172743468677204	38.0	35.0	40.0	30.0	41.0
25	36.23776394559527	38.0	35.0	40.0	30.0	41.0
26	36.103890614432785	38.0	35.0	40.0	30.0	41.0
27	36.063177867394735	38.0	35.0	40.0	30.0	41.0
28	36.00923809583448	38.0	35.0	40.0	30.0	41.0
29	35.66523369987225	38.0	34.0	40.0	29.0	41.0
30	35.77759687398242	38.0	34.0	40.0	29.0	41.0
31	35.57868114871127	38.0	34.0	40.0	29.0	41.0
32	35.535380607669765	38.0	34.0	40.0	29.0	41.0
33	35.52661529694662	38.0	34.0	40.0	29.0	41.0
34	35.357469378553716	38.0	34.0	40.0	28.0	41.0
35	35.36924204092879	38.0	34.0	40.0	28.0	41.0
36	34.98795337274253	37.0	34.0	40.0	26.0	41.0
37	35.0326315657641	37.0	34.0	40.0	27.0	41.0
38	34.94855252611277	37.0	33.0	40.0	27.0	41.0
39	34.873998071287225	37.0	33.0	40.0	26.0	41.0
40	34.66887195351051	37.0	33.0	40.0	25.0	41.0
41	34.83336986198432	37.0	34.0	40.0	26.0	41.0
42	34.7568412944919	37.0	33.0	40.0	26.0	41.0
43	34.60811280464895	37.0	33.0	40.0	26.0	41.0
44	34.46733712396363	37.0	33.0	40.0	25.0	41.0
45	34.163995378603815	36.0	33.0	40.0	24.0	41.0
46	33.99229923853418	36.0	33.0	39.0	23.0	41.0
47	33.890592515592516	36.0	33.0	39.0	23.0	41.0
48	33.870760589134086	36.0	33.0	39.0	23.0	41.0
49	33.75259562157152	36.0	33.0	39.0	23.0	40.0
50	33.456610224682514	35.0	32.0	39.0	22.0	40.0
51	31.374348746336697	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	4.0
10	6.0
11	16.0
12	14.0
13	15.0
14	44.0
15	67.0
16	146.0
17	287.0
18	510.0
19	915.0
20	1586.0
21	2379.0
22	3159.0
23	4179.0
24	5060.0
25	6041.0
26	7110.0
27	8722.0
28	11197.0
29	14281.0
30	18174.0
31	23607.0
32	30640.0
33	40922.0
34	64904.0
35	72331.0
36	65595.0
37	84279.0
38	101031.0
39	71543.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.911404453573127	38.25395135636099	18.388836009317938	24.445808180747942
2	21.690660771986074	37.76942489291887	18.01874859103775	22.52116574405731
3	19.908480074142727	29.563159081231372	25.22261603586905	25.30574480875686
4	24.425143401047013	30.758741828018938	17.61234125691957	27.20377351401448
5	16.890013275555443	38.19101770909	18.4295393632743	26.489429652080254
6	20.452026400821584	34.308387395736794	24.366436640533028	20.8731495629086
7	75.11850938055757	11.390363950604915	7.889092753550584	5.602033915286928
8	77.69910202139117	5.369242040928788	5.50935550935551	11.422300428324524
9	72.8715276908048	10.277440322621045	10.314386443904516	6.536645542669639
10	41.31985321744358	28.038661924204096	13.248472058713023	17.39301279963931
11	32.81504395962227	23.77983868947724	23.66054655211282	19.744570798787667
12	29.678850537284273	22.58989179169902	31.064799739498532	16.66645793151817
13	24.33309120056108	32.60792650852892	23.328344563284322	19.73063772762568
14	16.856667835583497	32.83179495528893	32.58303484207099	17.728502367056585
15	15.675957468126143	28.115215539914335	33.06380407284022	23.145022919119302
16	16.125886080705357	24.32823810835859	35.793277058337296	23.752598752598754
17	16.387639956917067	25.3929439170403	28.237951807228917	29.981464318813718
18	19.937128973273552	26.500544798737568	27.556796833905267	26.00552939408361
19	22.45447486411342	29.26649425143401	27.840624452070234	20.438406432382337
20	24.574493399794605	29.653176114019487	23.760739423390024	22.011591062795883
21	20.306277083385517	28.57688550459635	25.153420334143227	25.96341707787491
22	19.778542444205094	29.674153996443152	24.326359492022142	26.22094406732961
23	22.30762968714776	28.77523607945295	20.661335570974128	28.255798662425168
24	17.270433083686097	32.91742854995867	26.47064348871578	23.341494877639455
25	20.360600405781128	25.422845227062098	26.76198557222654	27.45456879493024
26	22.827067104175537	32.11745109335471	21.25826591188037	23.797215890589385
27	20.239429652080254	27.737457104926982	26.48301104626406	25.540102196728704
28	24.393520026050147	26.79548756355985	26.11558500112717	22.69540740926283
29	20.19418630864414	31.76051398942965	26.520896475715755	21.524403226210456
30	27.76610600405781	26.06627132229542	22.622297923502742	23.54532475014403
31	27.26341958269669	28.417359667359666	19.724845327254968	24.594375422688675
32	27.136143325902363	25.40656388547955	23.80128622598502	23.65600656263307
33	29.211388172231544	27.2980174335596	20.763563609949152	22.7270307842597
34	21.74842822433184	26.223448889111538	26.162237306815623	25.865885579741
35	29.556427372692433	25.027866142323973	23.684342359041153	21.73136412594244
36	25.65438469052927	26.90945695463768	25.96592189965684	21.470236455176213
37	25.440848633619716	27.264985096310397	23.447010495203266	23.847155774866618
38	24.85534654209353	28.064806001552988	23.572251584299778	23.507595872053702
39	21.705376599954914	25.813440873681838	29.840568093580146	22.640614432783106
40	25.75113343185632	27.732447461363126	23.314568043483703	23.201851063296846
41	21.521115647621674	20.742429176164116	32.220305337775216	25.516149838438995
42	29.339603737194096	23.58211432006613	22.191468577010745	24.88681336572903
43	23.43041605089798	23.526851689502294	26.969416126042635	26.073316133557096
44	23.843711644916464	22.50911128923177	26.238634371164494	27.40854269468727
45	27.766732209503292	25.9709315432207	23.40802920622198	22.854307041054028
46	20.881446785061243	24.93393532550159	32.76588683215189	21.418731057285274
47	23.27135986774541	26.029012098289208	24.424830298324274	26.27479773564111
48	22.87653733436866	20.869392330235705	32.66866843674073	23.58540189865491
49	24.488859805124864	20.475352553665807	29.20966610725647	25.82612153395286
50	21.112829697167047	24.80133632242066	25.304179295143154	28.781654685269142
51	20.11309270345415	23.701563008791922	30.40634471357363	25.778999574180293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3470.0
1	2809.0
2	2148.0
3	1531.0
4	914.0
5	839.5
6	765.0
7	870.0
8	975.0
9	1055.0
10	1135.0
11	1215.0
12	1295.0
13	1311.0
14	1327.0
15	1352.5
16	1378.0
17	1392.5
18	1407.0
19	1430.0
20	1453.0
21	1456.0
22	1459.0
23	1613.0
24	1767.0
25	1966.0
26	2885.5
27	3606.0
28	3983.0
29	4360.0
30	4878.5
31	5397.0
32	6536.5
33	7676.0
34	8727.5
35	9779.0
36	10881.0
37	11983.0
38	14113.5
39	16244.0
40	19435.0
41	22626.0
42	30391.5
43	38157.0
44	56364.0
45	74571.0
46	73896.5
47	73222.0
48	68812.0
49	64402.0
50	59666.0
51	54930.0
52	49770.5
53	44611.0
54	39995.5
55	35380.0
56	32245.5
57	29111.0
58	28028.5
59	26946.0
60	24745.5
61	22545.0
62	21482.5
63	20420.0
64	17954.5
65	15489.0
66	12805.5
67	10122.0
68	9270.5
69	8419.0
70	7185.0
71	5951.0
72	5135.0
73	4319.0
74	3603.0
75	2088.0
76	1289.0
77	1216.5
78	1144.0
79	896.5
80	649.0
81	515.0
82	381.0
83	322.0
84	263.0
85	167.0
86	71.0
87	90.0
88	109.0
89	68.5
90	28.0
91	21.0
92	14.0
93	10.5
94	7.0
95	3.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	638768.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.533491563420764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66592892134265	20.872817613631028
2	9.415923446414997	4.996746506545362
3	3.362185964483011	2.6763159876978504
4	1.5799086230435369	1.676819684820056
5	0.8640416385578094	1.1463020763558944
6	0.5886077320938881	0.9370690976206445
7	0.39677250185521545	0.7369431881400896
8	0.29037167859367763	0.6163659587377337
9	0.21977502899968646	0.5248258990032362
>10	2.9875374413094233	19.741887467556488
>50	1.220680777667074	23.178449928883282
>100	0.39885376735338607	14.23099040672771
>500	0.003529679357109612	0.7086540286554256
>1k	0.003529679357109612	1.8752796256093136
>5k	5.882798928516019E-4	0.8255663342639968
>10k+	0.001764839678554806	5.2549661957518845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	12131	1.8991245647872155	No Hit
AATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	11404	1.7853117250707613	RNA PCR Primer, Index 27 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGT	10131	1.5860218420459384	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	5289	0.828000150289307	RNA PCR Primer, Index 38 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2962	0.4637051323798312	No Hit
AATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	2455	0.38433359216491747	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	2123	0.3323585401898655	No Hit
AACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTG	1827	0.2860193372241565	RNA PCR Primer, Index 38 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1461	0.2287215389625028	RNA PCR Primer, Index 27 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTAT	1186	0.18566991458557724	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	933	0.1460624201588057	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	912	0.1427748415700223	No Hit
AATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	816	0.127745910878441	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	712	0.11146456929589459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.565513613706385E-4	0.0	0.0	1.112453973899757	0.0
2	1.565513613706385E-4	0.0	0.0	1.3648147684292262	0.0
3	1.565513613706385E-4	0.0	0.0	1.990237457104927	0.0
4	1.565513613706385E-4	0.0	0.0	5.478045237081382	0.0
5	1.565513613706385E-4	0.0	0.0	5.865353305112341	0.0
6	1.565513613706385E-4	0.0	0.0	7.544366655812439	0.0
7	3.13102722741277E-4	0.0	0.0	8.8908649149613	0.0
8	3.13102722741277E-4	0.0	0.0	9.74673120757458	0.0
9	3.13102722741277E-4	0.0	0.0	12.557924003707136	0.0
10	3.13102722741277E-4	0.0	0.0	14.124063822859004	0.0
11	3.13102722741277E-4	0.0	0.0	17.675274904190566	0.0
12	3.13102722741277E-4	0.0	0.0	18.529575683190142	0.0
13	3.13102722741277E-4	0.0	0.0	18.988897377451593	0.0
14	3.13102722741277E-4	0.0	0.0	19.543089196703654	0.0
15	3.13102722741277E-4	0.0	0.0	19.842728502367056	0.0
16	3.13102722741277E-4	0.0	0.0	20.356999724469603	0.0
17	3.13102722741277E-4	0.0	0.0	20.935613556095483	0.0
18	3.13102722741277E-4	0.0	0.0	21.830617689051422	0.0
19	3.13102722741277E-4	0.0	0.0	22.132761816496757	0.0
20	3.13102722741277E-4	0.0	0.0	22.473730681562007	0.0
21	3.13102722741277E-4	0.0	0.0	22.801549232272123	0.0
22	3.13102722741277E-4	0.0	0.0	23.164591839290633	0.0
23	3.13102722741277E-4	0.0	0.0	23.510100693835632	1.565513613706385E-4
24	3.13102722741277E-4	0.0	0.0	23.753068406682864	1.565513613706385E-4
25	4.696540841119154E-4	0.0	0.0	23.94359141347093	1.565513613706385E-4
26	4.696540841119154E-4	0.0	0.0	24.138810961100116	1.565513613706385E-4
27	4.696540841119154E-4	0.0	0.0	24.385692457981616	1.565513613706385E-4
28	4.696540841119154E-4	0.0	0.0	24.59030508729304	1.565513613706385E-4
29	4.696540841119154E-4	0.0	0.0	24.827636951130927	1.565513613706385E-4
30	4.696540841119154E-4	0.0	0.0	25.216823635498333	1.565513613706385E-4
31	4.696540841119154E-4	0.0	0.0	25.45681687247952	1.565513613706385E-4
32	4.696540841119154E-4	0.0	0.0	25.68663427097162	1.565513613706385E-4
33	4.696540841119154E-4	0.0	0.0	25.90940685820204	1.565513613706385E-4
34	4.696540841119154E-4	0.0	0.0	26.119185682438694	1.565513613706385E-4
35	4.696540841119154E-4	0.0	0.0	26.360118227588107	1.565513613706385E-4
36	4.696540841119154E-4	0.0	0.0	26.563321894647196	1.565513613706385E-4
37	4.696540841119154E-4	0.0	0.0	26.761515918142425	1.565513613706385E-4
38	4.696540841119154E-4	0.0	0.0	26.96878992059715	1.565513613706385E-4
39	4.696540841119154E-4	0.0	0.0	27.178099090749694	1.565513613706385E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTCG	20	7.0309296E-4	45.000004	6
AACGTAA	20	7.0309296E-4	45.000004	12
CGAACAC	20	7.0309296E-4	45.000004	33
ATAGCGA	20	7.0309296E-4	45.000004	40
ATTAACG	35	1.2107921E-7	45.000004	1
ATAGACG	35	1.2107921E-7	45.000004	1
TAAGTAC	20	7.0309296E-4	45.000004	32
AGGGTAC	80	0.0	45.000004	6
GCGCATT	20	7.0309296E-4	45.000004	26
ACTTACG	20	7.0309296E-4	45.000004	28
CTCGGTA	20	7.0309296E-4	45.000004	38
GCTTATT	20	7.0309296E-4	45.000004	38
TACGAAC	20	7.0309296E-4	45.000004	31
TGCGAGA	35	1.2107921E-7	45.000004	12
ACTACAT	20	7.0309296E-4	45.000004	32
GTACTTG	35	1.2107921E-7	45.000004	35
AATCTAG	40	6.8066583E-9	45.000004	1
AGTCTAG	20	7.0309296E-4	45.000004	31
TACTCGT	20	7.0309296E-4	45.000004	43
TCATCGG	20	7.0309296E-4	45.000004	2
>>END_MODULE