SRR3548734_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466161
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	10976	2.3545513245423795	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	10142	2.1756431790733246	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	9278	1.9902994888032246	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	4197	0.900332717666214	Illumina PCR Primer Index 5 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	2402	0.5152726204036803	RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2252	0.48309489639845465	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	1550	0.3325031480539985	No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1520	0.32606760325295336	Illumina PCR Primer Index 5 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1147	0.24605232955995893	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	1047	0.22460051355647515	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	779	0.16710964666713862	RNA PCR Primer, Index 33 (95% over 23bp)
AGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	735	0.15767084762560574	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	679	0.1456578306636548	RNA PCR Primer, Index 33 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	581	0.12463505098024073	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	568	0.12184631489978784	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	497	0.10661552553731438	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	486	0.10425582577693114	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	473	0.10146708969647825	RNA PCR Primer, Index 33 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTAC	30	2.1627147E-6	45.000004	13
GTCGCTA	30	2.1627147E-6	45.000004	11
ACTGATC	30	2.1627147E-6	45.000004	7
CATTGCG	35	1.2098462E-7	45.000004	1
TCTTAGG	35	1.2098462E-7	45.000004	2
CCGCTAT	35	1.2098462E-7	45.000004	24
ACCCTAC	35	1.2098462E-7	45.000004	11
TACATCC	30	2.1627147E-6	45.000004	23
GATTGCG	30	2.1627147E-6	45.000004	10
CAGTACG	35	1.2098462E-7	45.000004	1
TAGTGCG	35	1.2098462E-7	45.000004	1
TATGTCG	30	2.1627147E-6	45.000004	13
CACCCAT	60	0.0	45.000004	31
GTACCGC	25	3.8872255E-5	45.0	26
GTCGATG	20	7.028968E-4	45.0	8
GATGTAG	20	7.028968E-4	45.0	1
CGTATAT	20	7.028968E-4	45.0	10
GGTCGAT	20	7.028968E-4	45.0	7
TTTACGT	25	3.8872255E-5	45.0	13
ACCATTC	20	7.028968E-4	45.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content