##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466161
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40537282183623	31.0	31.0	34.0	30.0	34.0
2	31.57986403839017	31.0	31.0	34.0	30.0	34.0
3	31.734398630516065	31.0	31.0	34.0	30.0	34.0
4	35.53004219572208	37.0	35.0	37.0	33.0	37.0
5	35.127876420378364	37.0	35.0	37.0	32.0	37.0
6	35.12183987935499	37.0	35.0	37.0	32.0	37.0
7	35.01640205851626	37.0	35.0	37.0	32.0	37.0
8	35.360553542660156	37.0	35.0	37.0	33.0	37.0
9	36.28339994122202	39.0	37.0	39.0	32.0	39.0
10	36.04778606532936	38.0	35.0	39.0	31.0	39.0
11	36.295417248547174	38.0	35.0	39.0	32.0	39.0
12	35.88512552530135	37.0	35.0	39.0	31.0	39.0
13	35.74294503401185	37.0	35.0	39.0	31.0	39.0
14	36.68126462745704	38.0	35.0	40.0	31.0	41.0
15	36.79293420084477	38.0	35.0	40.0	31.0	41.0
16	36.724299115541626	38.0	35.0	40.0	31.0	41.0
17	36.67969864488878	38.0	35.0	40.0	31.0	41.0
18	36.43743041567184	38.0	35.0	40.0	31.0	41.0
19	36.41178262445808	38.0	35.0	40.0	30.0	41.0
20	36.49439571306909	38.0	35.0	40.0	31.0	41.0
21	36.62064608579439	38.0	35.0	40.0	31.0	41.0
22	36.57700022095371	38.0	35.0	40.0	31.0	41.0
23	36.53774554284893	38.0	35.0	40.0	31.0	41.0
24	36.18111124697261	38.0	35.0	40.0	30.0	41.0
25	36.19956838946201	38.0	35.0	40.0	30.0	41.0
26	36.130888255345255	38.0	35.0	40.0	30.0	41.0
27	36.10431803604334	38.0	35.0	40.0	30.0	41.0
28	35.84005096951483	38.0	34.0	40.0	30.0	41.0
29	35.70603503939626	38.0	34.0	40.0	29.0	41.0
30	35.647244192457116	38.0	34.0	40.0	29.0	41.0
31	35.208644652813085	37.0	34.0	40.0	27.0	41.0
32	35.40737856663256	37.0	34.0	40.0	29.0	41.0
33	35.07288683523504	37.0	34.0	40.0	27.0	41.0
34	35.20671827973597	37.0	34.0	40.0	27.0	41.0
35	35.102614761852664	37.0	34.0	40.0	27.0	41.0
36	34.802763422937566	37.0	33.0	40.0	25.0	41.0
37	34.92394044117805	37.0	34.0	40.0	26.0	41.0
38	34.458161450657606	37.0	33.0	40.0	24.0	41.0
39	34.66207597804192	37.0	33.0	40.0	26.0	41.0
40	34.43026336394507	37.0	33.0	40.0	25.0	41.0
41	34.69144136896909	37.0	33.0	40.0	26.0	41.0
42	34.72223759602369	37.0	33.0	40.0	26.0	41.0
43	34.670182619309635	37.0	33.0	40.0	26.0	41.0
44	34.53935013868599	37.0	33.0	40.0	26.0	41.0
45	34.165792505164525	36.0	33.0	40.0	24.0	41.0
46	34.10103161783161	36.0	33.0	39.0	24.0	41.0
47	33.940477646135136	36.0	33.0	39.0	23.0	41.0
48	33.96073245080562	36.0	33.0	39.0	23.0	40.0
49	33.83483388786278	36.0	33.0	39.0	23.0	40.0
50	33.54727872988088	35.0	33.0	39.0	23.0	40.0
51	31.404008486338412	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	6.0
11	8.0
12	8.0
13	14.0
14	24.0
15	44.0
16	87.0
17	177.0
18	391.0
19	679.0
20	1110.0
21	1601.0
22	2298.0
23	2850.0
24	3669.0
25	4271.0
26	5125.0
27	6234.0
28	8107.0
29	10420.0
30	13612.0
31	17606.0
32	23284.0
33	31428.0
34	48686.0
35	53161.0
36	49444.0
37	61617.0
38	71453.0
39	48738.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.81410070769541	40.74193250829649	17.79599752017007	22.64796926383803
2	21.590609253026315	38.94319773640438	18.08452444541693	21.381668565152385
3	18.405443612829046	29.124916069769885	27.27963085715021	25.190009460250856
4	24.955755629492813	30.069868564723347	17.619878110781467	27.354497695002372
5	16.80792687504961	37.98172734312823	18.64377328862775	26.566572493194414
6	20.49656663684864	33.94299394415234	24.88453560036125	20.675903818637767
7	72.96706502688986	12.667297350057169	8.50929185410191	5.85634576895107
8	75.4458223660924	5.591415841308046	5.7452253620530245	13.217536430546527
9	71.61817483659078	10.069911468355354	11.285800399432814	7.026113295621041
10	41.61094557459762	27.983035903904447	13.82741155952557	16.578606961972365
11	33.49808328024009	24.57777463151143	22.88801508491701	19.03612700333147
12	30.635338434575175	21.89629763107596	30.69497448306486	16.773389451284
13	25.04242096614689	31.960846145430445	21.819500129783485	21.17723275863918
14	17.82002355409397	31.13151893873576	33.112165110337415	17.936292396832854
15	15.618852713976503	28.410999633173944	30.08552839040589	25.884619262443664
16	16.99391411979981	25.040490302706576	34.771892114526956	23.19370346296666
17	17.28952014432782	26.434214788452913	27.3823850558069	28.893880011412364
18	21.594256061746908	25.870890100201432	25.870246545721326	26.664607292330334
19	21.810061330741952	29.58226878696416	28.638388882810872	19.96928099948301
20	25.067734109030997	30.44956570798501	23.346011356591394	21.1366888263926
21	20.802898569378392	27.781174315311663	25.65079446800569	25.765132647304256
22	20.13467450086987	30.604876855850232	24.80344773586808	24.457000907411818
23	22.6526886633588	27.70201711425881	20.474042230044983	29.17125199233741
24	17.689811030952825	33.499155871040266	25.94125205669286	22.869781041314052
25	19.917153086594546	26.761569500666077	25.637279823923492	27.683997588815878
26	24.409377875884083	30.992940207353254	20.747123847769334	23.85055806899333
27	19.95233406484026	27.75714828138776	26.510154217105246	25.780363436666732
28	21.956791752205785	29.46063699022441	26.39753218308696	22.18503907448285
29	24.37419689763837	28.630666229049623	25.874965945242096	21.120170928069914
30	27.868054170125774	26.778730953468866	22.57245887150577	22.780756004899594
31	27.86783965196574	29.048333086637452	22.654833844959146	20.42899341643767
32	30.135940158014073	24.05263417574615	19.87703819066803	25.934387475571747
33	25.951548928374528	30.609596255370995	22.529126203178727	20.90972861307574
34	24.000935299177755	25.40624376556597	25.980508879979237	24.612312055277037
35	25.884833780603696	27.404694944450526	22.408566997239152	24.30190427770663
36	27.976600359103397	24.92894085948846	28.904391401254077	18.19006738015407
37	27.99032952134563	26.282121412988218	21.800193495380352	23.9273555702858
38	25.441424743811687	28.651689008733033	26.237716153861008	19.669170093594275
39	25.87968534476286	24.088458708471965	27.24573698786471	22.786118958900467
40	23.457775317969542	29.212653997224137	22.111673863750937	25.217896821055387
41	20.364423450267182	21.16908106855786	33.12546523625958	25.341030244915387
42	25.890625770924636	23.717985846091803	21.492574453890395	28.89881392909317
43	23.865359822035735	22.589620324308555	27.29893749155335	26.246082362102364
44	22.913113709641088	22.332198532266748	25.215751639455036	29.53893611863712
45	27.358144503722965	26.288985994109332	23.673366068804555	22.679503433363152
46	21.11630960118929	23.333783821469407	33.428579396388805	22.121327180952505
47	24.19378712504907	26.306361965072156	23.62574303727682	25.874107872601954
48	22.06877023174397	20.632356632150696	34.170812230109334	23.128060905995998
49	24.059069720547193	20.402178646433313	27.884572068448456	27.65417956457104
50	21.146985698074268	23.449194591568148	25.267021479703367	30.136798230654215
51	20.64587127623289	21.91989462867979	30.89790866245782	26.5363254326295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2633.0
1	2122.5
2	1612.0
3	1134.5
4	657.0
5	601.0
6	545.0
7	648.5
8	752.0
9	783.0
10	814.0
11	879.0
12	944.0
13	1018.5
14	1093.0
15	1097.0
16	1101.0
17	1111.5
18	1122.0
19	1155.0
20	1188.0
21	1157.0
22	1126.0
23	1397.5
24	1669.0
25	1603.5
26	1829.5
27	2121.0
28	2919.0
29	3717.0
30	4011.0
31	4305.0
32	4959.0
33	5613.0
34	6830.5
35	8048.0
36	8273.0
37	8498.0
38	9217.5
39	9937.0
40	12458.5
41	14980.0
42	20733.0
43	26486.0
44	41628.5
45	56771.0
46	55731.5
47	54692.0
48	50558.5
49	46425.0
50	42023.0
51	37621.0
52	34706.5
53	31792.0
54	28143.5
55	24495.0
56	23403.0
57	22311.0
58	20493.5
59	18676.0
60	18008.0
61	17340.0
62	16384.5
63	15429.0
64	12932.0
65	10435.0
66	9577.0
67	8719.0
68	7450.5
69	6182.0
70	5358.5
71	4535.0
72	4312.0
73	4089.0
74	3275.5
75	1895.0
76	1328.0
77	1194.5
78	1061.0
79	798.0
80	535.0
81	436.0
82	337.0
83	246.0
84	155.0
85	139.5
86	124.0
87	104.5
88	85.0
89	56.5
90	28.0
91	19.5
92	11.0
93	17.0
94	23.0
95	12.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.696336540849547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42600324452151	21.998093159548763
2	8.620483543661505	4.775116267402088
3	2.9429626094273074	2.445278485735064
4	1.3809538990833958	1.5298945574564846
5	0.7333280096289754	1.0155249674757731
6	0.5319420291759499	0.8839707276167711
7	0.4226323164876666	0.8193756809336877
8	0.30107991916728566	0.6671048613559138
9	0.27475785142646847	0.6848807328313389
>10	4.052448643586429	27.613412555039346
>50	1.1841568289718516	22.10890572705451
>100	0.11764172418543885	5.200758878706578
>500	0.003869793558731541	0.7163851361238721
>1k	0.005417710982224157	3.025666126986372
>5k	7.739587117463081E-4	1.9888154676712404
>10k+	0.0015479174234926162	4.526816668062217
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	10976	2.3545513245423795	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	10142	2.1756431790733246	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	9278	1.9902994888032246	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	4197	0.900332717666214	Illumina PCR Primer Index 5 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	2402	0.5152726204036803	RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2252	0.48309489639845465	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	1550	0.3325031480539985	No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1520	0.32606760325295336	Illumina PCR Primer Index 5 (95% over 24bp)
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1147	0.24605232955995893	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	1047	0.22460051355647515	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	779	0.16710964666713862	RNA PCR Primer, Index 33 (95% over 23bp)
AGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	735	0.15767084762560574	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	679	0.1456578306636548	RNA PCR Primer, Index 33 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	581	0.12463505098024073	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	568	0.12184631489978784	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	497	0.10661552553731438	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	486	0.10425582577693114	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	473	0.10146708969647825	RNA PCR Primer, Index 33 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2158889310774603	0.0
2	0.0	0.0	0.0	1.4555057158363742	0.0
3	0.0	0.0	0.0	2.14217834610789	0.0
4	0.0	0.0	0.0	6.090385081549078	0.0
5	0.0	0.0	0.0	6.516203629218231	0.0
6	0.0	0.0	0.0	8.352479079116442	0.0
7	0.0	0.0	0.0	9.646881656766654	0.0
8	0.0	0.0	0.0	10.330121996477612	0.0
9	0.0	0.0	0.0	13.478390513148891	0.0
10	0.0	0.0	0.0	14.866323008574291	0.0
11	0.0	0.0	0.0	18.98035228172241	0.0
12	0.0	0.0	0.0	19.808392379456883	0.0
13	2.1451816003483775E-4	0.0	0.0	20.305216438097567	0.0
14	2.1451816003483775E-4	0.0	0.0	20.926461029558457	0.0
15	2.1451816003483775E-4	0.0	0.0	21.268188458493952	0.0
16	2.1451816003483775E-4	0.0	0.0	21.84266809106725	0.0
17	2.1451816003483775E-4	0.0	0.0	22.402560488758176	0.0
18	2.1451816003483775E-4	0.0	0.0	23.58391199607003	0.0
19	2.1451816003483775E-4	0.0	0.0	23.92027647100465	0.0
20	2.1451816003483775E-4	0.0	0.0	24.325930311630533	0.0
21	2.1451816003483775E-4	0.0	0.0	24.686106302329023	0.0
22	2.1451816003483775E-4	0.0	0.0	25.038774157426296	0.0
23	2.1451816003483775E-4	0.0	0.0	25.408174429006287	0.0
24	2.1451816003483775E-4	0.0	0.0	25.68318671017095	0.0
25	2.1451816003483775E-4	0.0	0.0	25.905642042127077	0.0
26	2.1451816003483775E-4	0.0	0.0	26.116084357121252	0.0
27	2.1451816003483775E-4	0.0	0.0	26.40074995548748	0.0
28	2.1451816003483775E-4	0.0	0.0	26.623634323763678	0.0
29	2.1451816003483775E-4	0.0	0.0	26.8645382174828	0.0
30	2.1451816003483775E-4	0.0	0.0	27.2875680290715	0.0
31	2.1451816003483775E-4	0.0	0.0	27.528686440950658	0.0
32	2.1451816003483775E-4	0.0	0.0	27.757362799547796	0.0
33	2.1451816003483775E-4	0.0	0.0	27.990544039505664	0.0
34	2.1451816003483775E-4	0.0	0.0	28.197554063939283	0.0
35	2.1451816003483775E-4	0.0	0.0	28.44575157509959	0.0
36	2.1451816003483775E-4	0.0	0.0	28.662843953054846	0.0
37	2.1451816003483775E-4	0.0	0.0	28.896025193012715	0.0
38	2.1451816003483775E-4	0.0	0.0	29.11654986152853	0.0
39	2.1451816003483775E-4	0.0	0.0	29.330638985243297	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTAC	30	2.1627147E-6	45.000004	13
GTCGCTA	30	2.1627147E-6	45.000004	11
ACTGATC	30	2.1627147E-6	45.000004	7
CATTGCG	35	1.2098462E-7	45.000004	1
TCTTAGG	35	1.2098462E-7	45.000004	2
CCGCTAT	35	1.2098462E-7	45.000004	24
ACCCTAC	35	1.2098462E-7	45.000004	11
TACATCC	30	2.1627147E-6	45.000004	23
GATTGCG	30	2.1627147E-6	45.000004	10
CAGTACG	35	1.2098462E-7	45.000004	1
TAGTGCG	35	1.2098462E-7	45.000004	1
TATGTCG	30	2.1627147E-6	45.000004	13
CACCCAT	60	0.0	45.000004	31
GTACCGC	25	3.8872255E-5	45.0	26
GTCGATG	20	7.028968E-4	45.0	8
GATGTAG	20	7.028968E-4	45.0	1
CGTATAT	20	7.028968E-4	45.0	10
GGTCGAT	20	7.028968E-4	45.0	7
TTTACGT	25	3.8872255E-5	45.0	13
ACCATTC	20	7.028968E-4	45.0	37
>>END_MODULE