SRR3548733_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470899
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	9961	2.115315598461666	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	9076	1.9273772082760847	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	8883	1.8863917740322236	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	4177	0.8870267297233589	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2330	0.4947982476072364	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	2133	0.4529633743116889	RNA PCR Primer, Index 33 (95% over 23bp)
AATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	1526	0.3240609982183016	No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1432	0.3040991805036749	Illumina PCR Primer Index 5 (95% over 24bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	1057	0.22446426940808964	No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1003	0.21299684221032536	RNA PCR Primer, Index 33 (96% over 25bp)
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	762	0.16181813934622924	RNA PCR Primer, Index 33 (95% over 23bp)
AATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	658	0.13973272400238693	RNA PCR Primer, Index 33 (95% over 24bp)
AGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	655	0.13909564471362224	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	632	0.13421137016642634	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	577	0.12253158320574051	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	484	0.10278212525403536	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	20	7.0290384E-4	45.000004	31
GTCGAAA	20	7.0290384E-4	45.000004	23
ACGTTAC	20	7.0290384E-4	45.000004	28
TTTGTCG	40	6.8012014E-9	45.000004	27
CGCATAG	20	7.0290384E-4	45.000004	1
TAGAACG	20	7.0290384E-4	45.000004	1
ATAGATC	20	7.0290384E-4	45.000004	36
ATTCGCG	20	7.0290384E-4	45.000004	1
CGTCTAG	20	7.0290384E-4	45.000004	16
AGGGTAC	60	0.0	45.000004	6
TAACGGT	20	7.0290384E-4	45.000004	20
CCTCGTA	20	7.0290384E-4	45.000004	20
TAACGAG	20	7.0290384E-4	45.000004	1
ATCGTAC	20	7.0290384E-4	45.000004	19
ATGTAGC	20	7.0290384E-4	45.000004	45
TAGTTCG	40	6.8012014E-9	45.000004	1
CGTTAAG	20	7.0290384E-4	45.000004	1
ACGATAG	45	3.8380676E-10	45.000004	1
TGCGCTA	20	7.0290384E-4	45.000004	19
TCGCTGA	20	7.0290384E-4	45.000004	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content