##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470899
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20071607712057	31.0	31.0	34.0	28.0	34.0
2	31.41429266148367	31.0	31.0	34.0	30.0	34.0
3	31.340879891441688	31.0	31.0	34.0	28.0	34.0
4	35.325262954476436	37.0	35.0	37.0	33.0	37.0
5	34.994453162992485	35.0	35.0	37.0	32.0	37.0
6	35.04153756962746	36.0	35.0	37.0	32.0	37.0
7	34.88720723552184	36.0	35.0	37.0	32.0	37.0
8	35.27796194088329	37.0	35.0	37.0	33.0	37.0
9	35.830137672834304	39.0	35.0	39.0	32.0	39.0
10	35.73321242984164	38.0	35.0	39.0	30.0	39.0
11	36.14044412920818	38.0	35.0	39.0	32.0	39.0
12	35.76343547130064	37.0	35.0	39.0	31.0	39.0
13	35.6094958791588	37.0	35.0	39.0	30.0	39.0
14	36.538002841373626	38.0	35.0	40.0	31.0	41.0
15	36.67709848608725	38.0	35.0	40.0	31.0	41.0
16	36.631298856017956	38.0	35.0	40.0	31.0	41.0
17	36.595660640604464	38.0	35.0	40.0	31.0	41.0
18	36.387422780681206	38.0	35.0	40.0	31.0	41.0
19	36.358677763172146	38.0	35.0	40.0	30.0	41.0
20	36.418427306067755	38.0	35.0	40.0	31.0	41.0
21	36.50480676323373	38.0	35.0	40.0	31.0	41.0
22	36.49080588406431	38.0	35.0	40.0	31.0	41.0
23	36.44239210531345	38.0	35.0	40.0	31.0	41.0
24	36.06907850728076	38.0	34.0	40.0	30.0	41.0
25	36.124929124929125	38.0	34.0	40.0	30.0	41.0
26	36.06164803917613	38.0	35.0	40.0	30.0	41.0
27	36.01135487652341	38.0	34.0	40.0	30.0	41.0
28	35.71082546363445	38.0	34.0	40.0	29.0	41.0
29	35.60105457858267	38.0	34.0	40.0	29.0	41.0
30	35.57453509138902	38.0	34.0	40.0	29.0	41.0
31	35.239361306777035	37.0	34.0	40.0	27.0	41.0
32	35.38640557741682	37.0	34.0	40.0	29.0	41.0
33	35.00657890545531	37.0	34.0	40.0	27.0	41.0
34	35.20875601774478	37.0	34.0	40.0	28.0	41.0
35	35.08148668822826	37.0	34.0	40.0	27.0	41.0
36	34.816429850137716	37.0	33.0	40.0	25.0	41.0
37	34.91777005260151	37.0	34.0	40.0	27.0	41.0
38	34.499644297397104	37.0	33.0	40.0	25.0	41.0
39	34.63285332948254	37.0	33.0	40.0	26.0	41.0
40	34.365562466686065	36.0	33.0	40.0	24.0	41.0
41	34.61629988596281	37.0	33.0	40.0	26.0	41.0
42	34.678958757610445	37.0	33.0	40.0	26.0	41.0
43	34.64708780439118	37.0	33.0	40.0	26.0	41.0
44	34.605424942503596	37.0	33.0	40.0	26.0	41.0
45	34.28501653220754	36.0	33.0	40.0	24.0	41.0
46	34.1170335889437	36.0	33.0	39.0	24.0	41.0
47	33.94098734548173	35.0	33.0	39.0	23.0	40.0
48	33.89826056118191	35.0	33.0	39.0	23.0	40.0
49	33.78841110301784	35.0	33.0	39.0	24.0	40.0
50	33.638480863199966	35.0	33.0	39.0	23.0	40.0
51	31.644654161508093	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	8.0
11	6.0
12	14.0
13	16.0
14	18.0
15	32.0
16	106.0
17	207.0
18	364.0
19	664.0
20	1122.0
21	1749.0
22	2297.0
23	2908.0
24	3701.0
25	4384.0
26	5251.0
27	6341.0
28	8305.0
29	10686.0
30	14074.0
31	18604.0
32	24009.0
33	33160.0
34	50456.0
35	53938.0
36	50774.0
37	62535.0
38	70075.0
39	45084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.130429242788793	39.96143546705345	18.090291123998988	22.817844166158775
2	21.52202489281141	38.47045757158117	18.22089237819575	21.786625157411674
3	18.892586308316645	29.32815741804506	26.675996338917685	25.103259934720608
4	24.966287887636202	30.196071769105476	17.7082559105031	27.12938443275522
5	16.997275424241717	37.85503897863449	18.3336554123071	26.8140301848167
6	20.269314651337123	34.110923998564445	24.74968092945621	20.870080420642218
7	73.64381746404219	12.549400189849628	7.998742830203505	5.808039515904684
8	76.24671107817174	5.653441608497788	5.569559502143772	12.530287811186689
9	71.55079964068727	11.027417768990802	10.561075729615055	6.860706860706861
10	42.30673668875916	28.01152688793138	13.654095676567588	16.02764074674187
11	34.168473494316196	24.657092072822408	22.381444853354964	18.792989579506433
12	31.27698296237622	22.189683987436794	29.83463545261298	16.698697597574004
13	24.97860475388565	32.10285008037817	21.763690303016144	21.154854862720033
14	18.072028184387733	31.478512377388785	32.43073355432906	18.018725883894422
15	15.77450790933937	28.185874253289985	30.30034041269996	25.739277424670682
16	17.191584607314944	25.074166647200357	34.830398875342695	22.903849870142004
17	17.516707404347855	26.219847568162173	27.41840607009146	28.845038957398504
18	21.93570171098261	25.903431521409047	25.716130210512233	26.44473655709611
19	22.26103686777844	29.256804537703413	28.417134035111562	20.065024559406584
20	25.435178244167005	30.26445161276622	23.01448930662414	21.285880836442633
21	21.023191809708663	27.87497956037282	25.567053656941297	25.53477497297722
22	20.438140662859762	30.511638376806914	24.607399888298765	24.442821072034555
23	22.294377350557127	28.081605609695497	20.690211701447655	28.933805338299724
24	17.928685344415683	32.934450911979	26.329637565592623	22.807226178012698
25	20.021490808007663	26.919148267462873	25.91362479002928	27.145736134500183
26	24.178857886723055	31.052731052731055	20.872204018271432	23.896207042274458
27	20.08795941380211	28.05591007838199	26.49124334517593	25.36488716263997
28	21.851819604628595	29.40651817056311	26.683641290382866	22.05802093442543
29	23.790239520576602	28.92637274659747	25.943567516601224	21.339820216224712
30	27.242359826629492	26.916812310070735	23.034238764575843	22.806589098723933
31	28.032975223986462	29.10263134982236	22.4655393194719	20.398854106719273
32	29.993055835752465	24.365522118331107	20.045912180743642	25.59550986517279
33	25.892388813737128	30.539669865512565	22.615677671857448	20.952263648892863
34	24.104107250174668	25.822947171261777	25.67917961176388	24.393765966799673
35	25.876674191280934	27.485299395411754	22.738846334351955	23.89918007895536
36	27.970116734161678	25.278244379367976	28.485089159246463	18.266549727223886
37	27.774108672985076	26.740341347082918	21.96288376063657	23.52266621929543
38	25.26146795809717	29.071839184198733	25.87051575815621	19.796177099547883
39	25.785359493224664	24.500158208023375	26.999420257847223	22.715062040904737
40	23.751802403487794	28.812335553908586	22.200301975582875	25.23556006702074
41	20.41308221083502	21.60548227963958	32.493167324628	25.4882681848974
42	25.701265027107723	23.871573309775556	21.670889086619425	28.756272576497295
43	24.004722881127375	22.959063408501613	27.047413564267497	25.988800146103518
44	22.808500336590225	22.816570007581245	25.332183759150052	29.04274589667848
45	26.799377361175114	26.78663577539982	23.63542925340678	22.778557610018286
46	21.327715709738182	23.71655068284282	32.79493054773954	22.160803059679466
47	24.36722099643448	26.476802881297264	23.63627869245847	25.51969742980979
48	22.305207698466127	20.97774682044345	33.60933023854372	23.107715242546703
49	23.975841953370043	20.88133548807706	27.66559283413216	27.477229724420738
50	21.23682573120775	23.94844754395316	25.31137250238374	29.503354222455346
51	20.835890498811846	22.402893189410044	30.599980038182284	26.161236273595822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2714.0
1	2201.5
2	1689.0
3	1191.5
4	694.0
5	654.5
6	615.0
7	651.0
8	687.0
9	761.5
10	836.0
11	932.0
12	1028.0
13	1054.5
14	1081.0
15	1062.0
16	1043.0
17	1070.0
18	1097.0
19	1124.0
20	1151.0
21	1221.0
22	1291.0
23	1495.0
24	1699.0
25	1669.0
26	1880.5
27	2122.0
28	3016.5
29	3911.0
30	4186.0
31	4461.0
32	5150.5
33	5840.0
34	7121.5
35	8403.0
36	8614.5
37	8826.0
38	9593.0
39	10360.0
40	13020.5
41	15681.0
42	21403.0
43	27125.0
44	41201.5
45	55278.0
46	54760.0
47	54242.0
48	50475.0
49	46708.0
50	41966.0
51	37224.0
52	34575.0
53	31926.0
54	28509.5
55	25093.0
56	24016.5
57	22940.0
58	21085.0
59	19230.0
60	18508.5
61	17787.0
62	16667.5
63	15548.0
64	13125.5
65	10703.0
66	9737.5
67	8772.0
68	7706.0
69	6640.0
70	5645.0
71	4650.0
72	4354.5
73	4059.0
74	3263.0
75	1928.0
76	1389.0
77	1218.0
78	1047.0
79	786.5
80	526.0
81	403.0
82	280.0
83	210.0
84	140.0
85	133.5
86	127.0
87	103.5
88	80.0
89	50.0
90	20.0
91	15.5
92	11.0
93	12.5
94	14.0
95	9.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.369579785382786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3750294647358	22.51836231366932
2	8.800484805579881	4.993321116838947
3	2.977027815590356	2.533710844130833
4	1.3800987113334096	1.5661128201150853
5	0.7918529887967081	1.1232268271981016
6	0.5091948220065141	0.8667385877530555
7	0.4137264121570521	0.8216071121306756
8	0.30799450980416276	0.6990139855479246
9	0.24278617170011524	0.6198967501950657
>10	3.9294533846514588	27.51549351899637
>50	1.1397206947310567	21.964814358010244
>100	0.12139036985301195	5.240943945631595
>500	0.0037466163534880226	0.6981122705221935
>1k	0.005245262894883232	2.9034157706431545
>5k	0.002247969812092814	5.935229778617431
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	9961	2.115315598461666	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	9076	1.9273772082760847	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	8883	1.8863917740322236	No Hit
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	4177	0.8870267297233589	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2330	0.4947982476072364	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	2133	0.4529633743116889	RNA PCR Primer, Index 33 (95% over 23bp)
AATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	1526	0.3240609982183016	No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	1432	0.3040991805036749	Illumina PCR Primer Index 5 (95% over 24bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	1057	0.22446426940808964	No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1003	0.21299684221032536	RNA PCR Primer, Index 33 (96% over 25bp)
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	762	0.16181813934622924	RNA PCR Primer, Index 33 (95% over 23bp)
AATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	658	0.13973272400238693	RNA PCR Primer, Index 33 (95% over 24bp)
AGCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	655	0.13909564471362224	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	632	0.13421137016642634	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	577	0.12253158320574051	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	484	0.10278212525403536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1235976292156066E-4	0.0	0.0	1.1454685611988982	0.0
2	2.1235976292156066E-4	0.0	0.0	1.3629249584305765	0.0
3	2.1235976292156066E-4	0.0	0.0	1.9940581738334546	0.0
4	2.1235976292156066E-4	0.0	0.0	5.497569542513363	0.0
5	2.1235976292156066E-4	0.0	0.0	5.887249707474426	0.0
6	2.1235976292156066E-4	0.0	0.0	7.5899502865795	0.0
7	2.1235976292156066E-4	0.0	0.0	8.800825654758238	0.0
8	2.1235976292156066E-4	0.0	0.0	9.426437516325157	0.0
9	2.1235976292156066E-4	0.0	0.0	12.423470850437143	0.0
10	2.1235976292156066E-4	0.0	0.0	13.717803605444056	0.0
11	2.1235976292156066E-4	0.0	0.0	17.558966997169243	0.0
12	2.1235976292156066E-4	0.0	0.0	18.383984676119507	0.0
13	2.1235976292156066E-4	0.0	0.0	18.847566038577273	0.0
14	2.1235976292156066E-4	0.0	0.0	19.462772271761036	0.0
15	2.1235976292156066E-4	0.0	0.0	19.776852361122025	0.0
16	2.1235976292156066E-4	0.0	0.0	20.314547280839417	0.0
17	2.1235976292156066E-4	0.0	0.0	20.863284908228728	0.0
18	2.1235976292156066E-4	0.0	0.0	22.001957957014138	0.0
19	2.1235976292156066E-4	0.0	0.0	22.326656034521204	0.0
20	4.247195258431213E-4	0.0	0.0	22.73162610241262	0.0
21	4.247195258431213E-4	0.0	0.0	23.09646017511186	0.0
22	4.247195258431213E-4	0.0	0.0	23.431776240765004	0.0
23	4.247195258431213E-4	0.0	0.0	23.784505806977716	0.0
24	4.247195258431213E-4	0.0	0.0	24.046557754422924	0.0
25	6.37079288764682E-4	0.0	0.0	24.26507595046921	0.0
26	6.37079288764682E-4	0.0	0.0	24.472551438843574	0.0
27	6.37079288764682E-4	0.0	0.0	24.744796654909013	0.0
28	6.37079288764682E-4	0.0	0.0	24.97669351601936	0.0
29	6.37079288764682E-4	0.0	0.0	25.212625212625213	0.0
30	6.37079288764682E-4	0.0	0.0	25.62163011601214	0.0
31	6.37079288764682E-4	0.0	0.0	25.85288989783372	0.0
32	6.37079288764682E-4	0.0	0.0	26.083937319892375	0.0
33	6.37079288764682E-4	0.0	0.0	26.313710583373506	0.0
34	6.37079288764682E-4	0.0	0.0	26.523097309614162	0.0
35	6.37079288764682E-4	0.0	0.0	26.777504305594192	0.0
36	6.37079288764682E-4	0.0	0.0	27.00303037381689	0.0
37	6.37079288764682E-4	0.0	0.0	27.212204740294627	0.0
38	6.37079288764682E-4	0.0	0.0	27.430935296103836	0.0
39	6.37079288764682E-4	0.0	0.0	27.639472583292807	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	20	7.0290384E-4	45.000004	31
GTCGAAA	20	7.0290384E-4	45.000004	23
ACGTTAC	20	7.0290384E-4	45.000004	28
TTTGTCG	40	6.8012014E-9	45.000004	27
CGCATAG	20	7.0290384E-4	45.000004	1
TAGAACG	20	7.0290384E-4	45.000004	1
ATAGATC	20	7.0290384E-4	45.000004	36
ATTCGCG	20	7.0290384E-4	45.000004	1
CGTCTAG	20	7.0290384E-4	45.000004	16
AGGGTAC	60	0.0	45.000004	6
TAACGGT	20	7.0290384E-4	45.000004	20
CCTCGTA	20	7.0290384E-4	45.000004	20
TAACGAG	20	7.0290384E-4	45.000004	1
ATCGTAC	20	7.0290384E-4	45.000004	19
ATGTAGC	20	7.0290384E-4	45.000004	45
TAGTTCG	40	6.8012014E-9	45.000004	1
CGTTAAG	20	7.0290384E-4	45.000004	1
ACGATAG	45	3.8380676E-10	45.000004	1
TGCGCTA	20	7.0290384E-4	45.000004	19
TCGCTGA	20	7.0290384E-4	45.000004	14
>>END_MODULE