FastQCFastQC Report
Sat 18 Jun 2016
SRR3548730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009623
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC59220.5865555756950862No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT52630.5212836870792366No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT50650.5016724064328962No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT43740.4332310179146077Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27420.27158652289022733No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16460.16303115123169737No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14670.14530176115243018No Hit
AAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT13530.13401041774999184No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT10690.1058811061158472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.032879E-445.041
TCGTTAC207.032879E-445.038
CGTATAC207.032879E-445.023
CGCATAT253.8904676E-545.043
CGCATAA207.032879E-445.042
ATTCGAT253.8904676E-545.033
TACGCTA207.032879E-445.043
CTAGTCA207.032879E-445.040
TGCGAAT207.032879E-445.044
TACTCGA207.032879E-445.016
TTACGTA207.032879E-445.026
ATGCGAA207.032879E-445.017
CAATGCG351.211738E-745.01
CGGTATG351.211738E-745.01
ATACGGA207.032879E-445.028
GTGCGTT207.032879E-445.023
ATCATAC253.8904676E-545.042
CGATAGA207.032879E-445.044
ATCGCAC207.032879E-445.032
GTTGCGA253.8904676E-545.016