##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009623
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.548005542662953	31.0	31.0	34.0	30.0	34.0
2	31.730454833140687	31.0	31.0	34.0	30.0	34.0
3	31.889672679802263	33.0	31.0	34.0	30.0	34.0
4	35.71045627922502	37.0	35.0	37.0	35.0	37.0
5	35.34398582441169	37.0	35.0	37.0	33.0	37.0
6	35.36283642508144	37.0	35.0	37.0	33.0	37.0
7	35.34891142535382	37.0	35.0	37.0	33.0	37.0
8	35.64231401226002	37.0	35.0	37.0	35.0	37.0
9	36.4976827984307	39.0	37.0	39.0	33.0	39.0
10	36.19864147310432	38.0	35.0	39.0	31.0	39.0
11	36.436626344685095	38.0	35.0	39.0	32.0	39.0
12	36.220124739630535	38.0	35.0	39.0	32.0	39.0
13	36.061587344979266	38.0	35.0	39.0	32.0	39.0
14	37.14623181128005	39.0	35.0	40.0	32.0	41.0
15	37.13141241829871	39.0	35.0	40.0	32.0	41.0
16	37.12091741174676	39.0	35.0	40.0	32.0	41.0
17	37.06889601366054	39.0	35.0	40.0	32.0	41.0
18	36.9290656017147	38.0	35.0	40.0	32.0	41.0
19	36.97714790570342	39.0	35.0	40.0	32.0	41.0
20	36.9381521617475	39.0	35.0	40.0	31.0	41.0
21	36.99752580913866	39.0	35.0	40.0	32.0	41.0
22	36.94873928189037	39.0	35.0	40.0	31.0	41.0
23	36.89322846250531	39.0	35.0	40.0	31.0	41.0
24	36.55276474486021	38.0	35.0	40.0	31.0	41.0
25	36.599409878736914	38.0	35.0	40.0	31.0	41.0
26	36.53258295423143	38.0	35.0	40.0	31.0	41.0
27	36.438320046195464	38.0	35.0	40.0	30.0	41.0
28	36.346012323411806	38.0	35.0	40.0	30.0	41.0
29	36.07543013580317	38.0	35.0	40.0	30.0	41.0
30	36.09330413431548	38.0	35.0	40.0	30.0	41.0
31	35.964949292953904	38.0	35.0	40.0	30.0	41.0
32	35.95232874052988	38.0	35.0	40.0	30.0	41.0
33	35.95843498018567	38.0	35.0	40.0	30.0	41.0
34	35.864590049949335	38.0	35.0	40.0	29.0	41.0
35	35.73777835885276	38.0	34.0	40.0	29.0	41.0
36	35.59264497738265	38.0	34.0	40.0	29.0	41.0
37	35.44104383517412	38.0	34.0	40.0	28.0	41.0
38	35.38752187697784	38.0	34.0	40.0	28.0	41.0
39	35.29477735748888	38.0	34.0	40.0	27.0	41.0
40	35.12335396479676	37.0	34.0	40.0	27.0	41.0
41	35.12083619331176	37.0	34.0	40.0	27.0	41.0
42	35.01184501541665	37.0	34.0	40.0	26.0	41.0
43	34.915182201673296	37.0	34.0	40.0	26.0	41.0
44	34.76984973599056	37.0	33.0	40.0	26.0	41.0
45	34.533164359369785	37.0	33.0	40.0	25.0	41.0
46	34.436763029368386	36.0	33.0	40.0	25.0	41.0
47	34.28839576752907	36.0	33.0	40.0	24.0	41.0
48	34.21627775912395	36.0	33.0	40.0	24.0	41.0
49	34.00414808299732	36.0	33.0	39.0	24.0	41.0
50	33.76440215803325	36.0	33.0	39.0	23.0	41.0
51	31.752447200588733	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	10.0
10	16.0
11	23.0
12	27.0
13	45.0
14	72.0
15	113.0
16	210.0
17	328.0
18	748.0
19	1232.0
20	2090.0
21	3105.0
22	4451.0
23	5929.0
24	7439.0
25	8617.0
26	10256.0
27	12554.0
28	15634.0
29	19979.0
30	25532.0
31	32702.0
32	42725.0
33	58065.0
34	93587.0
35	101229.0
36	102813.0
37	139060.0
38	182796.0
39	138221.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.503075900608444	33.32451816172968	19.96735415100488	25.205051786657002
2	24.81252903311434	31.210758867418832	20.281927016321934	23.694785083144897
3	20.81539346865117	30.467907327784726	22.01851582224256	26.698183381321545
4	22.107955147614504	32.29641163087608	18.82465038930373	26.770982832205686
5	18.730159673462275	35.08240204511981	17.78406395258428	28.403374328833635
6	21.698198238352333	37.794206352272084	19.451914229370765	21.055681180004814
7	82.7738670771169	7.308272493792237	5.457482644511862	4.460377784578997
8	84.58662292756802	5.143999294786272	4.297544727091202	5.971833050554514
9	77.44900819414772	9.760772090176234	7.848771274030009	4.94144844164604
10	42.67176956150959	28.937831249882386	12.211389795993158	16.17900939261487
11	32.932490642546774	27.125174446303223	21.003186337870673	18.939148573279333
12	31.478878749790763	23.74886467523026	26.529407511516673	18.2428490634623
13	25.743272488839892	29.785276286296963	23.71875442615709	20.75269679870605
14	21.107779834651154	30.725429194857885	28.237074630827546	19.92971633966342
15	20.06867910101097	26.782769409967877	31.285440208870043	21.863111280151106
16	21.018142415535305	25.2296154109009	30.316761801187177	23.43548037237662
17	20.733481705547515	24.86294389093751	27.320494877791017	27.083079525723956
18	24.36850190615705	24.87611712490702	26.206613755827668	24.548767213108256
19	24.962882184736284	28.815310269278733	24.78717303389483	21.434634512090156
20	27.087635681833717	27.927949343467812	23.0747516647303	21.909663309968177
21	24.829168907602146	27.911408515851953	23.94477938794976	23.31464318859614
22	22.589620085913257	27.17846166341298	24.52677880753509	25.705139443138673
23	23.54641286896198	28.345729049358027	22.047437508852312	26.06042057282768
24	21.543883211852343	28.88533640774824	26.059132963492317	23.511647416907103
25	22.44699259030351	26.71977559940691	25.039544463626523	25.793687346663063
26	24.90731688957165	28.633460212376306	22.77771009574861	23.681512802303434
27	22.319222125486444	26.900536140717872	25.591631727882586	25.188610005913098
28	22.748986502882758	26.979773638278843	28.15318193028487	22.11805792855353
29	23.270567330577848	27.743325974150746	25.53141122973625	23.454695465535156
30	24.812826173730194	26.372121078858147	26.19106339693133	22.62398935048033
31	27.997876435065365	27.26096770774834	22.360227530474248	22.38092832671205
32	26.145204695217917	25.365606766089915	23.93903466937659	24.550153869315576
33	26.136686664230112	25.38383139052894	25.768628488059402	22.710853457181543
34	24.600172539650938	25.978607856595975	24.99428004314482	24.426939560608265
35	23.626046554010756	25.273196034559437	28.189135944803155	22.911621466626652
36	27.393492422419065	25.83578226724233	26.18393202215084	20.586793288187767
37	23.554831853077832	28.80708937890678	24.34136306324242	23.296715704772968
38	24.899393139815555	27.518093387333685	25.217036458163093	22.36547701468766
39	25.32202614243138	24.90097788976677	24.757756112925318	25.019239854876524
40	26.039422635974024	25.502489543126494	24.249843753559496	24.208244067339987
41	23.98093149621195	23.62158944477295	26.959469029528844	25.438010029486254
42	25.658686460193557	25.5398302138521	24.53807015093753	24.26341317501681
43	23.325538344510775	24.61146388305338	26.888749562955677	25.174248209480172
44	23.88941218652903	23.729550535199774	25.294986346388704	27.086050931882493
45	24.710015520644834	26.18690342830938	25.021220792315546	24.081860258730238
46	23.41428434177906	24.029959697827803	28.933869375004335	23.621886585388804
47	24.53430636980338	25.983164012705736	25.385218046736256	24.097311570754627
48	23.875644671327812	23.042660478218107	28.93832648424214	24.14336836621194
49	23.862570484230254	23.868513296547324	27.689246382065384	24.579669837157038
50	22.940543153236405	24.549361494339966	26.15332653871792	26.35676881370571
51	23.153394881059562	24.94198329475458	27.3926010005715	24.512020823614357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3302.0
1	2685.5
2	2069.0
3	1495.0
4	921.0
5	919.5
6	918.0
7	1054.5
8	1191.0
9	1266.5
10	1342.0
11	1547.0
12	1752.0
13	1740.5
14	1729.0
15	1853.5
16	1978.0
17	1819.5
18	1661.0
19	1650.0
20	1639.0
21	1896.0
22	2153.0
23	3040.5
24	3928.0
25	4212.5
26	5136.5
27	5776.0
28	6876.0
29	7976.0
30	9255.5
31	10535.0
32	13198.0
33	15861.0
34	18306.5
35	20752.0
36	22331.5
37	23911.0
38	25777.5
39	27644.0
40	31515.0
41	35386.0
42	44791.0
43	54196.0
44	67543.5
45	80891.0
46	84794.0
47	88697.0
48	89701.5
49	90706.0
50	89700.0
51	88694.0
52	80692.5
53	72691.0
54	66543.0
55	60395.0
56	57586.5
57	54778.0
58	51934.5
59	49091.0
60	47319.0
61	45547.0
62	41817.5
63	38088.0
64	33420.0
65	28752.0
66	25487.0
67	22222.0
68	20348.5
69	18475.0
70	15721.5
71	12968.0
72	11074.5
73	9181.0
74	7954.0
75	5476.5
76	4226.0
77	3381.0
78	2536.0
79	2088.5
80	1641.0
81	1252.5
82	864.0
83	844.5
84	825.0
85	545.0
86	265.0
87	193.0
88	121.0
89	96.5
90	72.0
91	47.5
92	23.0
93	16.5
94	10.0
95	10.5
96	11.0
97	7.5
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1009623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.15072384516069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77086139812309	20.599150434677448
2	9.22425416170968	4.824418465208881
3	3.252617254750239	2.551748868089345
4	1.497557496703116	1.566488501541333
5	0.8170865202117917	1.0683701973830937
6	0.5261032371896884	0.8254788281875569
7	0.3816096126964917	0.6985557318799276
8	0.2962861572527337	0.6198477981968074
9	0.23353327267505294	0.5496357710143728
>10	3.141430459986278	20.467954764277454
>50	1.3692642011505085	25.96503963865192
>100	0.4796904289882479	16.316899102745435
>500	0.006346099060544912	1.1250778490609477
>1k	0.002239799668427616	1.2349986870689844
>5k	0.001119899834213808	1.5863353620165204
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	5922	0.5865555756950862	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	5263	0.5212836870792366	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT	5065	0.5016724064328962	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	4374	0.4332310179146077	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2742	0.27158652289022733	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1646	0.16303115123169737	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1467	0.14530176115243018	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	1353	0.13401041774999184	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT	1069	0.1058811061158472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6034925907987437	0.0
2	0.0	0.0	0.0	0.7859369289328789	0.0
3	0.0	0.0	0.0	1.075549982518227	0.0
4	0.0	0.0	0.0	2.651583809005936	0.0
5	0.0	0.0	0.0	2.880382083213239	0.0
6	0.0	0.0	0.0	3.9342408007741505	0.0
7	0.0	0.0	0.0	4.776238259231416	0.0
8	0.0	0.0	0.0	5.3831974905484525	0.0
9	0.0	0.0	0.0	6.621382436810572	0.0
10	0.0	0.0	0.0	7.723774121627578	0.0
11	0.0	0.0	0.0	9.394100570212842	0.0
12	0.0	0.0	0.0	9.957082990383539	0.0
13	0.0	0.0	0.0	10.272646324420105	0.0
14	0.0	0.0	0.0	10.59960004873106	0.0
15	0.0	0.0	0.0	10.814333667121291	0.0
16	0.0	0.0	0.0	11.201111702090781	0.0
17	0.0	0.0	0.0	11.63701698554807	0.0
18	0.0	0.0	0.0	12.212479311584621	0.0
19	0.0	0.0	0.0	12.459205069615093	0.0
20	0.0	0.0	0.0	12.727721139474834	0.0
21	0.0	0.0	0.0	13.01634372434067	0.0
22	0.0	0.0	0.0	13.312592918346748	0.0
23	0.0	0.0	0.0	13.637862845834535	0.0
24	0.0	0.0	0.0	13.88072577585891	0.0
25	0.0	0.0	0.0	14.083870910230848	0.0
26	0.0	0.0	0.0	14.285926529011324	0.0
27	0.0	0.0	0.0	14.498976350578385	0.0
28	0.0	0.0	0.0	14.703310047413737	0.0
29	0.0	0.0	0.0	14.93121689977348	0.0
30	0.0	0.0	0.0	15.247671655657607	0.0
31	0.0	0.0	0.0	15.47240900811491	0.0
32	0.0	0.0	0.0	15.705763438431969	0.0
33	0.0	0.0	0.0	15.970119539669758	0.0
34	0.0	0.0	0.0	16.19624354833438	0.0
35	0.0	0.0	0.0	16.452081618584362	0.0
36	0.0	0.0	0.0	16.661367659017277	0.0
37	0.0	0.0	0.0	16.8673851526758	0.0
38	0.0	0.0	0.0	17.092221552005057	0.0
39	0.0	0.0	0.0	17.322505529291625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.032879E-4	45.0	41
TCGTTAC	20	7.032879E-4	45.0	38
CGTATAC	20	7.032879E-4	45.0	23
CGCATAT	25	3.8904676E-5	45.0	43
CGCATAA	20	7.032879E-4	45.0	42
ATTCGAT	25	3.8904676E-5	45.0	33
TACGCTA	20	7.032879E-4	45.0	43
CTAGTCA	20	7.032879E-4	45.0	40
TGCGAAT	20	7.032879E-4	45.0	44
TACTCGA	20	7.032879E-4	45.0	16
TTACGTA	20	7.032879E-4	45.0	26
ATGCGAA	20	7.032879E-4	45.0	17
CAATGCG	35	1.211738E-7	45.0	1
CGGTATG	35	1.211738E-7	45.0	1
ATACGGA	20	7.032879E-4	45.0	28
GTGCGTT	20	7.032879E-4	45.0	23
ATCATAC	25	3.8904676E-5	45.0	42
CGATAGA	20	7.032879E-4	45.0	44
ATCGCAC	20	7.032879E-4	45.0	32
GTTGCGA	25	3.8904676E-5	45.0	16
>>END_MODULE