Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548729_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1033617 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC | 5324 | 0.5150844074739482 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT | 4830 | 0.46729107590142194 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT | 4810 | 0.4653561232061779 | No Hit |
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT | 4180 | 0.4044051133059924 | Illumina Single End Adapter 1 (95% over 21bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2736 | 0.2647015287093769 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1612 | 0.15595718723666502 | No Hit |
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA | 1532 | 0.14821737645568908 | No Hit |
AAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT | 1185 | 0.11464594719320599 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTACCGT | 25 | 3.8905313E-5 | 45.000004 | 10 |
GTCGATC | 20 | 7.032955E-4 | 45.000004 | 17 |
TCCGCAA | 20 | 7.032955E-4 | 45.000004 | 31 |
ACGTTAC | 25 | 3.8905313E-5 | 45.000004 | 22 |
ATTAACG | 25 | 3.8905313E-5 | 45.000004 | 17 |
CGGATTA | 25 | 3.8905313E-5 | 45.000004 | 16 |
GCGATAC | 20 | 7.032955E-4 | 45.000004 | 41 |
ATTGGAC | 20 | 7.032955E-4 | 45.000004 | 12 |
CGCAATA | 20 | 7.032955E-4 | 45.000004 | 31 |
CGATACG | 20 | 7.032955E-4 | 45.000004 | 42 |
TCGTTTA | 70 | 0.0 | 45.0 | 38 |
TAAGACG | 35 | 1.2117744E-7 | 45.0 | 1 |
GCGTAAG | 55 | 1.8189894E-12 | 45.0 | 1 |
AGGCGAT | 30 | 2.1652886E-6 | 44.999996 | 44 |
TACGAAT | 210 | 0.0 | 43.92857 | 11 |
CGAATAT | 210 | 0.0 | 43.92857 | 13 |
ACTACGG | 175 | 0.0 | 43.714287 | 2 |
GCGATGA | 110 | 0.0 | 42.954544 | 9 |
CGGGTCA | 150 | 0.0 | 42.0 | 6 |
TAGTAGG | 125 | 0.0 | 41.4 | 2 |