FastQCFastQC Report
Sat 18 Jun 2016
SRR3548729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033617
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC53240.5150844074739482No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT48300.46729107590142194No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT48100.4653561232061779No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT41800.4044051133059924Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27360.2647015287093769No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16120.15595718723666502No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA15320.14821737645568908No Hit
AAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT11850.11464594719320599No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT253.8905313E-545.00000410
GTCGATC207.032955E-445.00000417
TCCGCAA207.032955E-445.00000431
ACGTTAC253.8905313E-545.00000422
ATTAACG253.8905313E-545.00000417
CGGATTA253.8905313E-545.00000416
GCGATAC207.032955E-445.00000441
ATTGGAC207.032955E-445.00000412
CGCAATA207.032955E-445.00000431
CGATACG207.032955E-445.00000442
TCGTTTA700.045.038
TAAGACG351.2117744E-745.01
GCGTAAG551.8189894E-1245.01
AGGCGAT302.1652886E-644.99999644
TACGAAT2100.043.9285711
CGAATAT2100.043.9285713
ACTACGG1750.043.7142872
GCGATGA1100.042.9545449
CGGGTCA1500.042.06
TAGTAGG1250.041.42