##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548729_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033617
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33540663514629	31.0	31.0	34.0	30.0	34.0
2	31.55034892034477	31.0	31.0	34.0	30.0	34.0
3	31.480991508460097	31.0	31.0	34.0	28.0	34.0
4	35.49751600447748	37.0	35.0	37.0	33.0	37.0
5	35.202796587130436	37.0	35.0	37.0	33.0	37.0
6	35.261937448784224	37.0	35.0	37.0	32.0	37.0
7	35.20738822987625	37.0	35.0	37.0	32.0	37.0
8	35.562470431504124	37.0	35.0	37.0	33.0	37.0
9	36.00965057656753	39.0	37.0	39.0	32.0	39.0
10	35.87024787711503	38.0	35.0	39.0	30.0	39.0
11	36.25989897611978	38.0	35.0	39.0	32.0	39.0
12	36.10239576167962	37.0	35.0	39.0	32.0	39.0
13	35.94455683294682	37.0	35.0	39.0	31.0	39.0
14	37.01990485837597	39.0	35.0	40.0	32.0	41.0
15	37.03668089824374	39.0	35.0	40.0	32.0	41.0
16	37.03458340952209	39.0	35.0	40.0	32.0	41.0
17	36.98713643448202	38.0	35.0	40.0	32.0	41.0
18	36.86495287906449	38.0	35.0	40.0	31.0	41.0
19	36.90433497127079	39.0	35.0	40.0	31.0	41.0
20	36.860441536855525	39.0	35.0	40.0	31.0	41.0
21	36.8957882852159	39.0	35.0	40.0	31.0	41.0
22	36.86338846981038	39.0	35.0	40.0	31.0	41.0
23	36.80973223157127	39.0	35.0	40.0	31.0	41.0
24	36.43312464868515	38.0	35.0	40.0	30.0	41.0
25	36.5193354985454	38.0	35.0	40.0	31.0	41.0
26	36.45146413033067	38.0	35.0	40.0	31.0	41.0
27	36.35940004856731	38.0	35.0	40.0	30.0	41.0
28	36.2343624379243	38.0	35.0	40.0	30.0	41.0
29	35.97883838984846	38.0	35.0	40.0	30.0	41.0
30	36.028221285060134	38.0	35.0	40.0	30.0	41.0
31	35.94939808459033	38.0	35.0	40.0	30.0	41.0
32	35.92912558520226	38.0	35.0	40.0	30.0	41.0
33	35.88668626773747	38.0	35.0	40.0	30.0	41.0
34	35.860630194743315	38.0	35.0	40.0	29.0	41.0
35	35.69797226632302	38.0	34.0	40.0	29.0	41.0
36	35.59467384921107	38.0	34.0	40.0	29.0	41.0
37	35.43660949848928	38.0	34.0	40.0	28.0	41.0
38	35.38638586633153	38.0	34.0	40.0	28.0	41.0
39	35.25872542731012	38.0	34.0	40.0	27.0	41.0
40	35.043138802864114	37.0	34.0	40.0	26.0	41.0
41	35.052350145169825	37.0	34.0	40.0	27.0	41.0
42	34.99894641825744	37.0	34.0	40.0	27.0	41.0
43	34.92588937681946	37.0	34.0	40.0	26.0	41.0
44	34.8711921340303	37.0	34.0	40.0	26.0	41.0
45	34.65516530784614	37.0	33.0	40.0	26.0	41.0
46	34.465730536552705	36.0	33.0	40.0	25.0	41.0
47	34.292116905971945	36.0	33.0	40.0	24.0	41.0
48	34.1828965661362	36.0	33.0	40.0	24.0	41.0
49	33.97209507970554	36.0	33.0	39.0	24.0	41.0
50	33.836825439210074	36.0	33.0	39.0	24.0	41.0
51	31.96146444959787	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	18.0
10	22.0
11	38.0
12	30.0
13	34.0
14	66.0
15	96.0
16	215.0
17	425.0
18	698.0
19	1427.0
20	2216.0
21	3372.0
22	4533.0
23	5946.0
24	7235.0
25	8795.0
26	10508.0
27	13136.0
28	16231.0
29	20989.0
30	26942.0
31	34770.0
32	45000.0
33	62228.0
34	98069.0
35	105355.0
36	108825.0
37	144443.0
38	182585.0
39	129353.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.6103256815629	32.99287840660515	20.05133429500482	25.345461616827127
2	24.88503962299382	30.94927811752322	20.317487038235633	23.84819522124733
3	21.289800767595736	30.402653981116796	21.75331868574143	26.554226565546042
4	22.201260234690412	32.19383969110415	18.813932046396296	26.790968027809143
5	18.8643375641074	34.94901883386206	17.721651249931067	28.464992352099472
6	21.674856353949288	37.824358538994616	19.359395211185575	21.14138989587052
7	83.08599800506377	7.220566225207209	5.2914183880489585	4.402017381680062
8	84.96667527720616	5.166323696301435	4.177756364301284	5.689244662191121
9	76.93604110613505	10.741889887646972	7.466595460407481	4.855473545810489
10	42.95488561043404	28.848209733392544	12.187299550994227	16.00960510517919
11	33.23378001716303	27.084210108773366	20.707476753962055	18.974533120101547
12	31.567495503653674	23.95548834819861	26.298812809773832	18.178203338373887
13	25.683497852686248	29.785307323699207	23.796822227188603	20.73437259642595
14	21.163545104231062	30.83405168452144	27.98183466409705	20.020568547150443
15	20.149049406114646	26.68319116268405	31.390737574943135	21.77702185625817
16	21.207565278047866	25.105043744443055	30.389012564615324	23.29837841289375
17	20.94866860742422	24.712635337847576	27.306245930552613	27.03245012417559
18	24.42722981529909	24.950828014632112	26.2279935411279	24.393948628940894
19	25.15312731891987	28.76790919653992	24.62430474730969	21.454658737230524
20	27.186569106351772	27.90743573296492	23.003975360312378	21.90201980037093
21	25.01487494884469	27.731838775871527	24.012279209804017	23.241007065479767
22	22.642622944475566	27.04405984034705	24.544971686804686	25.768345528372695
23	23.533668660635417	28.333705811727167	22.23637962610909	25.89624590152832
24	21.656764546248755	28.771488859026118	26.110445164891832	23.461301429833295
25	22.450385394203074	26.919545634408102	25.057830898679107	25.57223807270972
26	24.701606107484686	28.717310183559285	22.875687996617703	23.705395712338323
27	22.45822195261881	27.02896720932415	25.60484202562458	24.907968812432458
28	22.697769096290017	27.131132711633033	28.174459204908587	21.99663898716836
29	23.119105045679394	27.915756029554466	25.603003820564098	23.36213510420204
30	24.73208161243478	26.562546862135587	26.20274240845497	22.502629116974664
31	28.154238949243286	27.27228751075108	22.226898357902396	22.346575182103233
32	26.224607373911223	25.479747333877057	24.118604860407675	24.17704043180404
33	25.999669123089113	25.584138031785468	25.680692171278142	22.735500673847277
34	24.528524588895113	26.258082055538946	24.87904126963856	24.334352085927378
35	23.645605674055282	25.4015752449892	28.048397036813444	22.904422044142077
36	27.273061491829175	26.05607299415548	25.99338052682957	20.677484987185775
37	23.49197043005291	28.9993295388911	24.328837470745935	23.179862560310056
38	24.745529533666726	27.722164012395307	25.089660870515868	22.4426455834221
39	25.314889364242266	25.14161435038317	24.576608163371926	24.966888122002636
40	25.88347521373971	25.553082041026805	24.456544348632036	24.106898396601448
41	23.77873041948807	23.887571508595542	26.881717309216086	25.451980762700305
42	25.54282679174201	25.697816502631056	24.524074197696052	24.23528250793089
43	23.425504804971283	24.79670903245593	26.780712778524347	24.997073384048445
44	24.007635323335432	24.027855579000732	25.248810729699684	26.715698367964148
45	24.49359869274596	26.519590912301172	25.081534069195843	23.905276325757026
46	23.46991196932713	24.269434423001943	28.73027436661742	23.530379241053502
47	24.618790132128247	26.102995597015145	25.44307998030218	23.835134290554432
48	23.944749360740005	23.28609146327895	28.60469593669609	24.16446323928496
49	23.88447558428315	24.152466532574447	27.54908249380573	24.41397538933667
50	22.97485432224896	24.90409890704197	26.133761344869523	25.987285425839552
51	23.129844033138	25.31130970175607	27.262225756735813	24.296620508370122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3326.0
1	2774.5
2	2223.0
3	1577.5
4	932.0
5	939.0
6	946.0
7	1054.5
8	1163.0
9	1277.5
10	1392.0
11	1575.0
12	1758.0
13	1779.0
14	1800.0
15	1936.5
16	2073.0
17	1875.0
18	1677.0
19	1674.5
20	1672.0
21	1963.5
22	2255.0
23	3117.5
24	3980.0
25	4368.0
26	5415.5
27	6075.0
28	7303.0
29	8531.0
30	9769.0
31	11007.0
32	13686.5
33	16366.0
34	19066.0
35	21766.0
36	23437.5
37	25109.0
38	27136.0
39	29163.0
40	33141.0
41	37119.0
42	46226.0
43	55333.0
44	68675.5
45	82018.0
46	85836.5
47	89655.0
48	90791.0
49	91927.0
50	90768.0
51	89609.0
52	81774.5
53	73940.0
54	67712.0
55	61484.0
56	59060.5
57	56637.0
58	53703.5
59	50770.0
60	48684.5
61	46599.0
62	42701.0
63	38803.0
64	34129.0
65	29455.0
66	26183.5
67	22912.0
68	21045.5
69	19179.0
70	16121.5
71	13064.0
72	11331.0
73	9598.0
74	8194.5
75	5572.5
76	4354.0
77	3443.0
78	2532.0
79	2119.5
80	1707.0
81	1292.5
82	878.0
83	840.5
84	803.0
85	515.5
86	228.0
87	173.0
88	118.0
89	94.0
90	70.0
91	48.5
92	27.0
93	22.5
94	18.0
95	15.0
96	12.0
97	6.5
98	1.0
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1033617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.375049897561336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83873534583701	19.75161793363685
2	9.374322022858204	4.7574777817167
3	3.484525564775973	2.6526003022655527
4	1.5978106970189982	1.6217810465485736
5	0.9455911715423638	1.1997211580290479
6	0.6014192533707757	0.915662613818251
7	0.4246196538816734	0.7542321433312879
8	0.3351989959032227	0.6804552997325386
9	0.24611514492388284	0.562066567469016
>10	3.214354931818031	20.317057375451252
>50	1.413627661615178	25.949098230624035
>100	0.5144652534085579	17.158493749447207
>500	0.005758939403827141	1.026830513096798
>1k	0.0030714343487078088	2.134229226794265
>5k	3.839292935884761E-4	0.5186760580386482
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	5324	0.5150844074739482	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT	4830	0.46729107590142194	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	4810	0.4653561232061779	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	4180	0.4044051133059924	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2736	0.2647015287093769	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1612	0.15595718723666502	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1532	0.14821737645568908	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	1185	0.11464594719320599	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.934952695243983E-4	0.0	0.0	0.5440119502678459	0.0
2	1.934952695243983E-4	0.0	0.0	0.7055805003207184	0.0
3	1.934952695243983E-4	0.0	0.0	0.9752161584029675	0.0
4	1.934952695243983E-4	0.0	0.0	2.3698333135000684	0.0
5	1.934952695243983E-4	0.0	0.0	2.5853870437502477	0.0
6	1.934952695243983E-4	0.0	0.0	3.572019423055155	0.0
7	1.934952695243983E-4	0.0	0.0	4.32278106880982	0.0
8	1.934952695243983E-4	0.0	0.0	4.877145015997222	0.0
9	1.934952695243983E-4	0.0	0.0	6.046146686828874	0.0
10	1.934952695243983E-4	0.0	0.0	7.0767992399505815	0.0
11	1.934952695243983E-4	0.0	0.0	8.608314298236193	0.0
12	1.934952695243983E-4	0.0	0.0	9.132783226282076	0.0
13	1.934952695243983E-4	0.0	0.0	9.419059477543422	0.0
14	1.934952695243983E-4	0.0	0.0	9.734650262137716	0.0
15	1.934952695243983E-4	0.0	0.0	9.940335733642153	0.0
16	1.934952695243983E-4	0.0	0.0	10.29220688127227	0.0
17	1.934952695243983E-4	0.0	0.0	10.69516078005683	0.0
18	1.934952695243983E-4	0.0	0.0	11.236560544186096	0.0
19	1.934952695243983E-4	0.0	0.0	11.469335353423947	0.0
20	1.934952695243983E-4	0.0	0.0	11.727458042969495	0.0
21	1.934952695243983E-4	0.0	0.0	12.002414820963665	0.0
22	1.934952695243983E-4	0.0	0.0	12.30097802183981	0.0
23	1.934952695243983E-4	0.0	0.0	12.622857402693647	0.0
24	1.934952695243983E-4	0.0	0.0	12.85834114570484	0.0
25	1.934952695243983E-4	0.0	0.0	13.069444484755959	0.0
26	1.934952695243983E-4	0.0	0.0	13.257425139098912	0.0
27	1.934952695243983E-4	0.0	0.0	13.46543255383764	0.0
28	1.934952695243983E-4	0.0	0.0	13.65854083282299	0.0
29	1.934952695243983E-4	0.0	0.0	13.88202786912367	0.0
30	1.934952695243983E-4	0.0	0.0	14.193361757788427	0.0
31	1.934952695243983E-4	0.0	0.0	14.408721992769081	0.0
32	1.934952695243983E-4	0.0	0.0	14.625630189905932	0.0
33	1.934952695243983E-4	0.0	0.0	14.873400882531925	0.0
34	1.934952695243983E-4	0.0	0.0	15.087406650625908	0.0
35	1.934952695243983E-4	0.0	0.0	15.333242390556657	0.0
36	2.9024290428659744E-4	0.0	0.0	15.542894515086342	0.0
37	2.9024290428659744E-4	0.0	0.0	15.745677557547912	0.0
38	2.9024290428659744E-4	0.0	0.0	15.947880114200908	0.0
39	2.9024290428659744E-4	0.0	0.0	16.178139484934942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	25	3.8905313E-5	45.000004	10
GTCGATC	20	7.032955E-4	45.000004	17
TCCGCAA	20	7.032955E-4	45.000004	31
ACGTTAC	25	3.8905313E-5	45.000004	22
ATTAACG	25	3.8905313E-5	45.000004	17
CGGATTA	25	3.8905313E-5	45.000004	16
GCGATAC	20	7.032955E-4	45.000004	41
ATTGGAC	20	7.032955E-4	45.000004	12
CGCAATA	20	7.032955E-4	45.000004	31
CGATACG	20	7.032955E-4	45.000004	42
TCGTTTA	70	0.0	45.0	38
TAAGACG	35	1.2117744E-7	45.0	1
GCGTAAG	55	1.8189894E-12	45.0	1
AGGCGAT	30	2.1652886E-6	44.999996	44
TACGAAT	210	0.0	43.92857	11
CGAATAT	210	0.0	43.92857	13
ACTACGG	175	0.0	43.714287	2
GCGATGA	110	0.0	42.954544	9
CGGGTCA	150	0.0	42.0	6
TAGTAGG	125	0.0	41.4	2
>>END_MODULE