SRR3548728_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419098
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	9629	2.2975533168853106	No Hit
AATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	9303	2.21976721435082	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGT	8129	1.9396418021560589	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	5369	1.2810846150542354	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4109	0.980438942681664	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	2082	0.4967811824442016	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	2067	0.4932020672969091	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	1786	0.42615331020429587	No Hit
AACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1662	0.39656595832001107	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	1582	0.3774773442011176	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1039	0.24791337586912848	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	980	0.23383552295644455	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTAT	896	0.21379247813160646	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	853	0.20353234804270123	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	834	0.19899880218946403	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	752	0.17943297271759828	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	560	0.13362029883225401	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	479	0.11429307703687444	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	448	0.10689623906580323	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCC	20	7.0281513E-4	45.0	3
GTTGAGC	25	3.886549E-5	45.0	9
TTGATGT	20	7.0281513E-4	45.0	20
CTAGAGT	20	7.0281513E-4	45.0	27
TGTCAGA	20	7.0281513E-4	45.0	29
AGCCGTT	35	1.2094642E-7	45.0	45
TTGGCGA	20	7.0281513E-4	45.0	6
TCGTTAG	20	7.0281513E-4	45.0	16
GCCCAAC	25	3.886549E-5	45.0	1
ACACGAC	40	6.7975634E-9	45.0	26
CTCGTTA	20	7.0281513E-4	45.0	15
GGTAATT	45	3.8380676E-10	45.0	8
CAAGTGC	20	7.0281513E-4	45.0	36
ATCTACG	35	1.2094642E-7	45.0	1
TGCCATT	25	3.886549E-5	45.0	19
TTGTGCA	25	3.886549E-5	45.0	15
GGTAACA	20	7.0281513E-4	45.0	8
CAAGTCA	20	7.0281513E-4	45.0	35
TGCTTAC	25	3.886549E-5	45.0	20
TCATTAT	20	7.0281513E-4	45.0	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content