##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548728_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419098 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.117826856725635 31.0 31.0 34.0 28.0 34.0 2 31.284783034039773 31.0 31.0 34.0 28.0 34.0 3 31.47620127034727 31.0 31.0 34.0 30.0 34.0 4 35.304489642040764 37.0 35.0 37.0 33.0 37.0 5 34.8439672821154 35.0 35.0 37.0 32.0 37.0 6 34.8213615908451 35.0 35.0 37.0 32.0 37.0 7 34.70011071396189 35.0 35.0 37.0 32.0 37.0 8 35.081257844227366 36.0 35.0 37.0 32.0 37.0 9 35.9602073977924 39.0 35.0 39.0 32.0 39.0 10 35.772229884179836 37.0 35.0 39.0 30.0 39.0 11 36.04785038344254 37.0 35.0 39.0 31.0 39.0 12 35.460154904103575 37.0 35.0 39.0 30.0 39.0 13 35.17479682556347 37.0 34.0 39.0 30.0 39.0 14 36.032548473149475 38.0 34.0 40.0 30.0 41.0 15 36.25763425260917 38.0 35.0 40.0 31.0 41.0 16 36.24012522130862 38.0 34.0 40.0 31.0 41.0 17 36.16359180907568 38.0 35.0 40.0 31.0 41.0 18 35.88693575249703 37.0 34.0 40.0 30.0 41.0 19 35.79672057609437 37.0 34.0 40.0 29.0 41.0 20 35.85278622183833 37.0 34.0 40.0 30.0 41.0 21 35.94364325289073 37.0 34.0 40.0 30.0 41.0 22 35.896828426764145 37.0 34.0 40.0 30.0 41.0 23 35.82288867997461 37.0 34.0 40.0 30.0 41.0 24 35.4724980792082 37.0 34.0 40.0 29.0 41.0 25 35.537494810283036 37.0 34.0 40.0 29.0 41.0 26 35.44909782437521 37.0 34.0 40.0 29.0 41.0 27 35.26913991476934 37.0 34.0 40.0 28.0 41.0 28 35.21075977456347 37.0 34.0 40.0 27.0 41.0 29 34.89775422454891 36.0 33.0 40.0 27.0 41.0 30 34.666159227674676 36.0 33.0 40.0 25.0 41.0 31 34.37198459548841 36.0 33.0 39.0 25.0 41.0 32 34.336109931328714 36.0 33.0 39.0 25.0 41.0 33 34.25514318846666 36.0 33.0 39.0 25.0 41.0 34 34.14331015657436 36.0 33.0 39.0 24.0 41.0 35 33.96578604526865 36.0 33.0 39.0 23.0 41.0 36 33.59454351965412 35.0 32.0 39.0 22.0 40.0 37 33.274716176168816 35.0 32.0 39.0 21.0 40.0 38 33.3335711456509 35.0 32.0 39.0 22.0 40.0 39 33.31478556328114 35.0 32.0 39.0 22.0 40.0 40 33.18107220745506 35.0 32.0 39.0 22.0 40.0 41 33.16126061207641 35.0 32.0 39.0 22.0 40.0 42 33.18959765973591 35.0 32.0 39.0 21.0 40.0 43 32.98604145092556 35.0 32.0 39.0 21.0 40.0 44 32.823561553622305 35.0 31.0 39.0 20.0 40.0 45 32.52174431755819 35.0 31.0 39.0 20.0 40.0 46 32.35871800867577 35.0 31.0 39.0 19.0 40.0 47 32.332802828932614 35.0 31.0 39.0 18.0 40.0 48 32.325083870598284 35.0 31.0 39.0 18.0 40.0 49 32.16417401180631 35.0 31.0 38.0 18.0 40.0 50 31.819710425723816 35.0 30.0 38.0 15.0 40.0 51 29.623128719297156 34.0 26.0 36.0 10.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 6.0 10 11.0 11 21.0 12 11.0 13 19.0 14 23.0 15 59.0 16 115.0 17 281.0 18 520.0 19 1041.0 20 1730.0 21 2518.0 22 3453.0 23 4468.0 24 5239.0 25 5808.0 26 6749.0 27 7999.0 28 9875.0 29 11895.0 30 14945.0 31 18956.0 32 23975.0 33 31524.0 34 49495.0 35 52982.0 36 41791.0 37 47823.0 38 48411.0 39 27351.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.56773356112413 40.658986680919504 18.15923721897981 22.614042538976562 2 18.00724412905812 41.51582684718132 18.645758271335104 21.831170752425447 3 17.281876792540167 31.80210833742943 28.031629833595005 22.884385036435393 4 22.987702160353905 32.66610673398584 17.64670792988752 26.69948317577273 5 15.825176927592114 41.55185660633074 18.718056397310416 23.904910068766732 6 20.109616366577747 35.426320335577834 24.56012674839775 19.90393654944667 7 66.59086896143623 15.951877603806269 10.27516237252385 7.1820910622336545 8 69.00939637030002 8.832540360488478 7.402325947630387 14.755737321581112 9 65.90797379133282 12.287340908331702 14.16804661439568 7.636638685939805 10 42.05364854998115 25.574686588816935 13.915361084996828 18.45630377620509 11 34.38193453559788 22.063574629322975 25.009902218574176 18.54458861650497 12 29.824766522388558 22.908961627113467 31.594519658886465 15.67175219161151 13 23.11559587495049 36.47881879655832 22.234894941040043 18.170690387451145 14 12.71898219509518 36.044075610000526 35.45590768746212 15.781034507442174 15 11.619716629523404 27.762003159165637 36.45615106729213 24.16212914401882 16 13.132966513798683 25.22560355811767 37.86345914320756 23.77797078487609 17 13.007458875966957 26.37951028160478 28.643181308429057 31.969849533999206 18 19.303838243083955 26.044982319171172 27.879875351349803 26.77130408639507 19 20.77008241509146 31.899937484788758 27.761525943812664 19.568454156307116 20 22.356584856047988 32.110389455449564 23.662246061780298 21.87077962672215 21 15.953070642188699 32.944084677092235 25.3217624517416 25.78108222897747 22 16.294518227240406 33.845305871180486 24.161413320989364 25.69876258058974 23 20.835222310772185 30.559200950612986 19.337481925468506 29.268094813146327 24 14.590143594099708 34.40245479577569 26.526731217996748 24.480670392127855 25 18.017981474499997 26.868656018401428 26.875575641019523 28.237786866079055 26 22.518599468382096 33.217767682021865 20.550563352724183 23.713069496871853 27 16.058058019842612 31.299839178426048 25.184324430085564 27.457778371645773 28 24.5109735670416 28.374509064705627 26.01324749819851 21.10126987005426 29 22.699702694835096 30.420808498251006 25.424602360307137 21.45488644660676 30 24.340607686030474 30.7849238125689 22.431269058788157 22.443199442612467 31 29.494294890455215 35.25881774668454 15.185708354609185 20.061179008251052 32 28.336331836467842 26.290032402922463 17.055676715231282 28.31795904537841 33 28.149024810426198 28.123732396718665 23.19576805424984 20.53147473860529 34 23.069067378035687 30.22324134212046 19.504030083655852 27.203661196188005 35 28.87892569279739 27.381901130523172 23.201733246161997 20.537439930517447 36 27.332032126137562 30.27931414609471 25.463972626927355 16.924681100840377 37 21.818047330218707 34.29770602579826 19.02419004624217 24.86005659774086 38 24.463252031744364 32.37667562240812 23.406458632587128 19.753613713260382 39 21.098883793289396 26.96409908899589 26.15426463500184 25.782752482712873 40 25.976263308343157 27.223465633336357 22.935924294556404 23.864346763764082 41 20.84977737903784 23.915170198855638 29.154517559138917 26.080534862967614 42 30.9953757832297 22.234656333363557 21.46347632296026 25.30649156044648 43 23.510014364182126 23.34585228275964 27.22084094889501 25.923292404163227 44 22.229884179833835 24.128246853957787 23.94237147397506 29.699497492233316 45 26.044982319171172 29.236598599850154 22.904189473583745 21.81422960739493 46 19.511426921626924 23.810421428878207 33.35329684226601 23.32485480722886 47 22.558446950355286 25.740996139327795 24.80469961679607 26.895857293520848 48 22.240860132952196 19.73714978358284 35.16146581467819 22.860524268786776 49 24.95120473015858 17.726641501510386 29.516962619721404 27.80519114860963 50 20.38783291736062 22.033032846732745 24.858147736328974 32.72098649957766 51 18.83855327393593 21.630501696500577 32.91855365570821 26.61239137385528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4761.0 1 3830.5 2 2900.0 3 2085.5 4 1271.0 5 1238.5 6 1206.0 7 1285.0 8 1364.0 9 1490.5 10 1617.0 11 1679.0 12 1741.0 13 1840.0 14 1939.0 15 1965.0 16 1991.0 17 1890.0 18 1789.0 19 1692.0 20 1595.0 21 1522.5 22 1450.0 23 1441.0 24 1432.0 25 1309.5 26 1540.5 27 1894.0 28 1759.5 29 1625.0 30 1898.5 31 2172.0 32 2918.5 33 3665.0 34 4123.5 35 4582.0 36 4787.5 37 4993.0 38 5869.5 39 6746.0 40 9098.0 41 11450.0 42 18259.0 43 25068.0 44 42497.0 45 59926.0 46 57600.5 47 55275.0 48 51146.5 49 47018.0 50 43075.0 51 39132.0 52 34295.0 53 29458.0 54 25758.5 55 22059.0 56 19387.5 57 16716.0 58 15079.0 59 13442.0 60 12570.0 61 11698.0 62 10475.0 63 9252.0 64 8553.5 65 7855.0 66 6788.5 67 5722.0 68 4823.0 69 3924.0 70 3392.0 71 2860.0 72 2445.5 73 2031.0 74 1765.5 75 1353.5 76 1207.0 77 905.5 78 604.0 79 533.0 80 462.0 81 337.5 82 213.0 83 188.5 84 164.0 85 115.5 86 67.0 87 53.0 88 39.0 89 25.5 90 12.0 91 11.0 92 10.0 93 9.5 94 9.0 95 6.5 96 4.0 97 2.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 419098.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.963625411505102 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4765798446125 25.723232525522754 2 8.908045486935723 5.69466858186124 3 3.2662311859795823 3.132017704080823 4 1.6247805699151143 2.0773551005063395 5 0.9280820601973238 1.483243366164259 6 0.584241037229438 1.1204676998418577 7 0.4114344917729576 0.9205656583482655 8 0.32177679637628787 0.8228122388388653 9 0.23366843765074086 0.6722001370403924 >10 2.1835969106678106 16.522597021377397 >50 0.7816150830236583 17.81732973647329 >100 0.26725711530321844 11.693211726501454 >500 0.0044791695302215375 1.1632602882763567 >1k 0.005225697785258461 3.418672851309818 >5k 0.002986113020147692 7.738365363856873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC 9629 2.2975533168853106 No Hit AATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT 9303 2.21976721435082 No Hit AATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGT 8129 1.9396418021560589 No Hit CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT 5369 1.2810846150542354 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4109 0.980438942681664 No Hit AATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT 2082 0.4967811824442016 No Hit AAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT 2067 0.4932020672969091 No Hit AATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT 1786 0.42615331020429587 No Hit AACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG 1662 0.39656595832001107 No Hit AAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT 1582 0.3774773442011176 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1039 0.24791337586912848 No Hit ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA 980 0.23383552295644455 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTAT 896 0.21379247813160646 No Hit AAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT 853 0.20353234804270123 No Hit AATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC 834 0.19899880218946403 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 752 0.17943297271759828 No Hit GAAAAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT 560 0.13362029883225401 No Hit CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC 479 0.11429307703687444 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 448 0.10689623906580323 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3860767648616792E-4 0.0 0.0 1.7365866694663301 0.0 2 2.3860767648616792E-4 0.0 0.0 2.1310051586979655 0.0 3 2.3860767648616792E-4 0.0 0.0 3.0350896449040556 0.0 4 2.3860767648616792E-4 0.0 0.0 7.958520441519645 0.0 5 2.3860767648616792E-4 0.0 0.0 8.62948522779875 0.0 6 2.3860767648616792E-4 0.0 0.0 11.39828870574424 0.0 7 2.3860767648616792E-4 0.0 0.0 13.68534328486416 0.0 8 2.3860767648616792E-4 0.0 0.0 15.263971672496648 0.0 9 2.3860767648616792E-4 0.0 0.0 19.17904642828169 0.0 10 2.3860767648616792E-4 0.0 0.0 21.92446635393154 0.0 11 2.3860767648616792E-4 0.0 0.0 26.78919966213153 0.0 12 2.3860767648616792E-4 0.0 0.0 28.228719774372582 0.0 13 2.3860767648616792E-4 0.0 0.0 29.00157003851128 0.0 14 2.3860767648616792E-4 0.0 0.0 29.776567771738353 0.0 15 2.3860767648616792E-4 0.0 0.0 30.192460951853743 0.0 16 2.3860767648616792E-4 0.0 0.0 30.939541586931934 0.0 17 2.3860767648616792E-4 0.0 0.0 31.739115910837082 0.0 18 2.3860767648616792E-4 0.0 0.0 33.10729232780877 0.0 19 2.3860767648616792E-4 0.0 0.0 33.510539301070395 0.0 20 2.3860767648616792E-4 0.0 0.0 33.99849199948461 0.0 21 4.7721535297233584E-4 0.0 0.0 34.4022161880992 0.0 22 4.7721535297233584E-4 0.0 0.0 34.76227517191683 0.0 23 4.7721535297233584E-4 0.0 0.0 35.098950603438816 0.0 24 4.7721535297233584E-4 0.0 0.0 35.36070322454414 0.0 25 4.7721535297233584E-4 0.0 0.0 35.588334947911946 0.0 26 4.7721535297233584E-4 0.0 0.0 35.80833122563219 0.0 27 4.7721535297233584E-4 0.0 0.0 36.06578890856077 0.0 28 4.7721535297233584E-4 0.0 0.0 36.28912569375182 0.0 29 4.7721535297233584E-4 0.0 0.0 36.521768178325836 0.0 30 4.7721535297233584E-4 0.0 0.0 37.063407603949436 0.0 31 4.7721535297233584E-4 0.0 0.0 37.30082224205317 0.0 32 4.7721535297233584E-4 0.0 0.0 37.502207121007494 0.0 33 4.7721535297233584E-4 0.0 0.0 37.73938315143475 0.0 34 4.7721535297233584E-4 0.0 0.0 37.950073729772036 0.0 35 4.7721535297233584E-4 0.0 0.0 38.1972712826117 0.0 36 4.7721535297233584E-4 0.0 0.0 38.37789729371173 0.0 37 4.7721535297233584E-4 0.0 0.0 38.57307837307742 0.0 38 4.7721535297233584E-4 0.0 0.0 38.794983512209555 0.0 39 4.7721535297233584E-4 0.0 0.0 39.01736586669466 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCCC 20 7.0281513E-4 45.0 3 GTTGAGC 25 3.886549E-5 45.0 9 TTGATGT 20 7.0281513E-4 45.0 20 CTAGAGT 20 7.0281513E-4 45.0 27 TGTCAGA 20 7.0281513E-4 45.0 29 AGCCGTT 35 1.2094642E-7 45.0 45 TTGGCGA 20 7.0281513E-4 45.0 6 TCGTTAG 20 7.0281513E-4 45.0 16 GCCCAAC 25 3.886549E-5 45.0 1 ACACGAC 40 6.7975634E-9 45.0 26 CTCGTTA 20 7.0281513E-4 45.0 15 GGTAATT 45 3.8380676E-10 45.0 8 CAAGTGC 20 7.0281513E-4 45.0 36 ATCTACG 35 1.2094642E-7 45.0 1 TGCCATT 25 3.886549E-5 45.0 19 TTGTGCA 25 3.886549E-5 45.0 15 GGTAACA 20 7.0281513E-4 45.0 8 CAAGTCA 20 7.0281513E-4 45.0 35 TGCTTAC 25 3.886549E-5 45.0 20 TCATTAT 20 7.0281513E-4 45.0 42 >>END_MODULE